Advanced SearchSearch Tips
Genetic Analysis of Haimen Chicken Populations Using Decamer Random Markers
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Genetic Analysis of Haimen Chicken Populations Using Decamer Random Markers
Olowofeso, O.; Wang, J.Y.; Zhang, P.; Dai, G.J.; Sheng, H.W.; Wu, R.; Wu, X.;
  PDF(new window)
Through a screening and selection approach method, decamer random markers were used in a technique called random amplified polymorphic DNA (RAPD) assay with 252 genomic DNAs isolated from four major Haimen chicken populations: Rugao (62), Jiangchun (62), Wan-Nan (63) and Cshiqishi (65). A total of 3-score decamer random primers (S241-S260, S1081-S1100 and S1341-S1360) were employed in the preliminary RAPD-polymerase chain reaction (RAPD-PCR) assay with 50 random template DNA samples from all the populations. Four (6.67%) of the primers that produced obvious polymorphic patterns, interpretable and reproducible bands were selected and used with both the individual DNAs from each population and with pooled DNA samples of the four populations in subsequent analyses. The selected primers produced a total of 131 fragments with molecular size ranging from 835 to 4,972 base pairs (bp) when used with the individual DNAs; 105 (80.15%) of these fragments were polymorphic. With the pooled DNAs, 47 stable and characteristic bands with molecular size ranging from 840 to 4,983 bp, of which 23 (48.94%) polymorphic, were also generated. The band-sharing coefficient (BSC) calculated for the individuals in the population and among populations of bulked samples was between 0.8247 (Rugao) and 0.9500 (Cshiqishi); for pairwise populations, it was between 0.7273 (Rugao vs. Wan-Nan) and 0.9367 (Jiangchun vs. Cshiqishi) chicken populations. Using the BSC for individual and pairwise populations, the Nei's standard genetic distances between the chicken populations were determined and ranged from 0.0043 (Jiangchun vs. Cshiqishi) to 0.1375 (Rugao vs. Cshiqishi). The reconstructed dendrogram linked the Jiangchun and Cshiqishi chickens as closely related populations, followed by Wan-Nan, while the Rugao was the most genetically distant among the populations.
Genetic Analysis;Haimen Chickens;Random Markers;
 Cited by
Bartfai, R., S. Egedi, G. H. Yue, B. Kovacs, B. Urbanyi, G. Tamas, L. Horvath and L. Orban. 2003. Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers. J. Aquac. 219:157-167 crossref(new window)

Chen, H., F. Leibenguth, W. B. Sun and C. Z. Lei. 2002. Studies on RAPD in six animal species. In: Proceedings of the 8th National Symposium on Animal Genetic Markers. 8(1):133-139, Yangling, China

Jeffreys, A. J., V. Wilson and S. L. Thein. 1985. Individual specific fingerprints of human DNA. Nature, 316:76-79 crossref(new window)

Levin, I., L. B. Critterden and J. B. Dodgson. 1993. Genetic map of the chicken Z chromosome using random amplified polymorphic DNA (RAPD) markers. Genom. 16:224-230 crossref(new window)

Lynch, M. and B. G. Milligan. 1994. Analysis of population genetic structure with RAPD markers. Mol. Ecol. 3:91-99 crossref(new window)

Miller, S. A., D. D. Dykes and H. F. Polesky. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16:1215 crossref(new window)

Murayama, S., H. Yamagishi and T. Terachi. 1999. Identification of RAPD and SCAR markers linked to a restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) by bulked segregant analysis. J. Breed. Sci. 49:115-121 crossref(new window)

Mohd-Azmi, M. L., A. S. Ali and W. K. Kheng. 2000. DNA fingerprinting of red jungle fowl, village chickens and broilers. Asian-Aust. J. Anim. Sci. 13(8):1040-1043 crossref(new window)

Nei, M. 1975. Molecular Population Genetics and Evolution. North-Holland Publishing Company, Amsterdam-Oxford

Sandra, L. B. 2003. Phylogeny for the faint of heart: a tutorial, J. Trends in Genetics, 19(6):345-351

Smith, E. J., C. P. Jones, J. Bartlett and K. E. Nestor. 1996. Use of randomly amplified polymorphic DNA markers for the genetic analysis of relatedness and diversity in chickens and turkeys. Poult. Sci. 75:579-584 crossref(new window)

Wang, J. Y. and G. J. Dai. 2001. Study on relation between randomly amplified polymorphic DNA and growth performance in New Yangzhou chickens. Chinese J. Anim. Sci. 37(6):17-19

Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18(22):6531-6535 crossref(new window)

Welsh, J., C. Peterson and M. McClelland. 1990. Fingerprinting genomes using PCR arbitrary primers. Nucleic Acids Res. 16:7213-7218

Zhang, X., F. C. Leung, D. K. O. Chan, G. Yang and C. Wu. 2002. Genetic diversity of Chinese native chicken breeds based on protein polymorphism, randomly amplified polymorphic DNA and microsatellite polymorphism. Poult. Sci. 81:1463-1472 crossref(new window)

Zhang, J. Q., S. M. Geng, Z. J. Sun, X. F. Zhang, Y. L. Gu and L. J. Zhang. 2002. Analysis on RAPD markers of two chicken populations. In: Proceedings of the 8th National Symposium on Animal Genetic Markers, 8(1):209-212, Yangling, China