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Effect of Dietary Protein Levels on the Performance, Nutrient Balances, Metabolic Profile and Thyroid Hormones of Crossbred Calves
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Effect of Dietary Protein Levels on the Performance, Nutrient Balances, Metabolic Profile and Thyroid Hormones of Crossbred Calves
Lohakare, J.D.; Pattanaik, A.K.; Khan, S.A.;
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An experiment was conducted to study the effect of different dietary protein levels on the performance, nutrient balances, blood biochemical parameters and thyroid hormones of crossbred calves. Thirty crossbred (Bos taurusBos indicus) calves aged 3-5 months were divided into 3 equal groups of 10 each and fed graded levels of crude protein, namely 100 (NP), 75 (LP) and 125 (HP) percent of the Kearl recommendations for 105 d. The calves had access to ad libitum oat hay as the basal roughage. A metabolism trial of 6 d duration was conducted at 90 d of the study. Blood collection and its analysis for various hematological and biochemical parameters as well as thyroid hormones was done both during the pre- and post-experimental periods. The fortnightly body weight changes and the net gain did not differ significantly due to dietary variation. The average daily gain was , and in calves fed NP, LP and HP diets, respectively. Averaged across the feeding trial, oat hay intake was higher (p<0.05) in NP animals than HP or LP fed groups. The dry matter (DM) intake showed no significant difference between the 3 groups but the DM digestibility was higher (p<0.05) in the HP fed animals. The digestibility of crude protein, organic matter, crude fiber and nitrogen-free extract was significantly higher (p<0.05) on HP diets compared to LP or NP diets. The calves on all 3 diets were in positive nitrogen (N) balance, however the N retention was higher (p<0.05) in HP than in LP fed calves. The intake and retention of calcium and phosphorus were similar between the treatments. The blood biochemical profile revealed no significant influence of the dietary treatments on hemoglobin, packed cell volume as well as serum levels of glucose, total protein, albumin, globulin, Ca, P, and alkaline phosphatase. Serum levels of the circulating thyroid hormones ( and ) tended to be lower (p>0.05) on feeding of the LP diet besides showing an increasing trend with the advancement of age. Considering the similar performance and metabolic profile, it could be concluded that crossbred calves can be satisfactorily reared on 25% lower protein level as recommended by Kearl for developing countries, which would not only economize the cost of production but also help to reduce environmental pollution attributable to livestock production.
Protein;Calves;Growth;Nutrient Balances;Blood;Thyroid Hormones;
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Al Jassim, R. A. M., S. A. Hassan, Al Ani and T. K. Dana. 1991. Effect of undegradable protein supplementation on digestion and nitrogen balance in sheep and goats. Small Ruminant Res. 5:269-275

Allen, M. S. 2000. Effects of diet on short-term regulation of feed intake by lactating dairy cattle. J. Dairy Sci. 83:1598-1624

Andersen, H. R., J. Foldager and S. Mastrup. 1994. Effect of protein level and source on growth, feed conversion, carcass and meat quality in young cattle given mainly concentrates. Forskningsprapport-fra-statens-Husdyrbrugsforsag. 18:17-25

AOAC. 1990. Official Method of Analysis, 15th edn. Association of Official Analytical Chemists. Arlington, VA

Baginski, E. S., S. S. Marie, W. L. Clark and B. Zak. 1973. Direct microdetermination of serum calcium. Clin. Chem. Acta. 46:46-54 crossref(new window)

Benjamin, M. M. 1985. Outline of Veterinary Clinical Pathology, 3rd edn. Kalyani Publishers, New Delhi, India, pp. 233-254

Brosh, A., Y. Aharoni, D. Levy and Z. Holzer. 2000. Effect of dietary protein concentration and source on the growth rate and on body composition of Holstein-Friesian male calves. Anim. Sci. 70:527-536

Bunting, L. D., J. A. Boling, C. T. Mackown and G. M. Davenport. 1989. Effect of dietary protein level on nitrogen metabolism in the growing bovine. 2. Diffusion into and utilization of endogenous urea nitrogen in the rumen. J. Anim. Sci. 67:820-826

Dabiri, N. and M. L. Thonney. 2004. Source and level of supplemental protein for growing lambs. J. Anim. Sci. 82:3237-3244

Daly, J. A. and G. Ertingshausen. 1972. Direct method for determining inorganic phosphate in serum with 'CentrifiChem'. Clin. Chem. 18:263-265

Duncan, J. R. and K. W. Prasse. 1986. Effect of lasalocid on feedlot performance, energy partitioning and hormonal status of cattle. J. Anim. Sci. 53:417-423

Eicher, R., A. Liesegang, E. Bouchard and A. Tremblay. 1999. Effect of cow specific factors and feeding frequency of concentrate on diurnal variations of blood metabolites in dairy cows. Am. J. Vet. Res. 60:1493-1499

Gonzalez, F., A. Elias and V. Urquiza. 1990. Effect of different protein levels on the feed of grazing calves. Cuban J. Agric. Sci. 24:159-164

Hallford, D. M., R. E. Hudgens, D. G. Morrical, H. M. Schoenemann, H. E. Kierling and G. S. Smith. 1982. Influence of short-term consumption of sewage sludge on productivity of tall lambing ewes and performance of their offsprings. J. Anim. Sci. 49:922-932

Hultman, E. 1959. Raped specific method for determination of aldohexoses (aldosaccharides) in body fluids. Nature. 103:108-109 crossref(new window)

ICAR (Indian Council of Agriculture Research). 1997. Nutrient Requirement of Livestock and Poultry, 2nd edn. ICAR, New Delhi, India

Islam, M. R., M. Ishida, S. Ando and T. Nishida. 2002. In situ dry matter, nitrogen and phosphorus disappearance of different feeds for ruminants. Asian-Aust. J. Anim. Sci. 15:793-799

Jain, N. C. 1986. Veterinary Hematology, 4th edn. Lea and Febiger. Pheladelphia, USA

Kakkar, V. K., N. S. Malik and G. S. Makkar. 1991. Protein requirement of male buffalo calves. Indian J. Anim. Nutr. 8:191-194

Kaneko, J. J., J. W. Harvey and M. L. Bruss. 1997. Clinical Biochemistry of Domestic Animals, Academic Press, California, USA

Kearl, L. C. 1982. Nutrient requirement of ruminants in developing countries. International Feedstuffs Institute, Utah Agricultural Experiment Station, Utah State University, Loga, Utah (USA), p. 79

Kertz, A. F., L. R. Prewitt and J. M. Ballam. 1987. Increased weight gain and effects on growth parameters of Holstein heifers calves from 3 to 12 months of age. J. Dairy Sci. 70:1612-1622

Kind, P. R. M. and E. J. King. 1954. Estimation of serum alkaline phosphatase activity by colorimetric method. J. Clin. Path. 7:322-327 crossref(new window)

Khang, D. N. and H. Wiktorsson. 2004. Effects of ensiled cassava tops on rumen environment parameters, thyroid gland hormones and liver enzymes of cows fed urea-treated fresh rice straw. Asian-Aust. J. Anim. Sci. 17:936-941

Kishan, J., M. Y. Khan and M. Lal. 1987. Influence of dietary energy levels on urinary nitrogen and carbon excretion in buffaloes. Indian J. Anim. Nutr. 3:197-200

Kolbikava, V. V. 1978. Supply of energy materials to the blood of cattle. Vesisi Akademii Navuk BSSR, Selskagaspadarchykh Navuk. No. 1:104-106 (cited in Nutr. Abst. Rev. Ser. B. 48. Abstr. No. 4725)

McDonald, P., R. A. Edwards, J. F. D. Greenhalgh and C. A. Morgan. 1995. Animal Nutrition. 5th edn. Longman Scientific and Rechnical Publications, Essex, England

Melvin, J. S. 1982. Duke's Physiology of Domestic Animals. 9th edn. Comstock Publishing Associates, A division of Cornell University Press, Ithaca and London

Moss, R. J. and R. M. Murray. 1992. Rearing dairy calves on irrigated tropical pastures. 1. Effect of protein level on live weight gain and blood components. Aust. J. Exptl. Agric. 32:569-579 crossref(new window)

Murphy, J. J. 1999. Effect of dry period protein feeding on postpartum milk production and composition. Livest. Prod. Sci. 57: 169-179 crossref(new window)

Neville, W. E. Jr., R. E. Hellwig, R. J. Ritter and W. C. McCormick. 1977. Effect of diet on protein level on weight gains of early weaned beef calves. J. Anim. Sci. 44:687-693

Oldham, J. D. 1984. Protein-energy interrelationships in dairy cows. J. Dairy Sci. 67:1090-1114

Patil, N. V. and H. Kaur. 1994. Influence of varying levels of dietary protein and proportions of rumen degradable protein on nutrient utilization in pregnant crossbred goats. Indian J. Anim. Nutr. 11:139-142

Pattanaik, A. K., A. Sahoo and T. K. Goswami. 2002. Immunomodulatory response of farm animals to dietary intervention Annual Report 2001-02, Indian Veterinary Research Institute, Izatnagar, India, pp. 48-49

Pattanaik, A. K., V. R. B. Sastry, R. C. Katiyar and Murari Lal. 2003. Influence of grain processing and dietary protein degradability on nitrogen metabolism, energy balance and methane production in young calves. Asian-Aust. J. Anim. Sci. 16:1443-1450

Promkot, C. and M. Wanapat. 2005. Effect of level of crude protein and use of cottonseed meal in diets containing cassava chips and rice straw for lactating dairy cows. Asian-Aust. J. Anim. Sci. 18:502-511

Radostits, O. M., D. C. Blood and C. C. Gay. 1994. Veterinary Medicine, ELBS, Bailliere Tindall, London

Rahamatulla, M. and T. R. C. Boyde. 1980. Improvement in the determination of urea using diacetyl monoxime method with or without deprotenization. Clin. Chemica. Acta. 107:3-9 crossref(new window)

Ranjhan, S. K. 1991. Chemical Composition of Indian Feeds and Feeding of Farm Animals. Indian Counc. Agric. Res. New Delhi, India

Roffler, R. E., J. E. Wray and L. D. Satter. 1986. Production responses in early lactation to additions of soybean meal to diets containing predominantly corn silage. J. Dairy Sci. 69:1055-1062

Sampath, K. T., C. S. Prasad and M. T. Shivaramaiah. 1991.Effect of different levels of rumen degradable protein on growth and nutrient utilization in crossbred calves. Indian J. Anim. Nutr. 8:89-92

Sen, K. C., S. N. Ray and S. K. Ranjhan. 1978. Chemical Composition of Indian Feeds Feeding of farm Animals. Indian Counc. Agric. Res. New Delhi, India

Sengar, S. S., D. C. Joshi and S. B. Johri. 1985. Effect of feeding different levels of protein on nutrient utilization and growth in male buffalo calves. Indian J. Anim. Nutr. 2:27-30

Shetaewi, M. M. and T. T. Ross. 1991. Effects of concentrate supplementation and lasalocid on serum chemistry and hormone profiles in Rambouillet ewes. Small Ruminant Res. 4:365-377 crossref(new window)

Shukla, D. C., V. P. Varshney, P. S. P. Gupta and B. B. Mahapatra. 1994. Plasma concentration of L-thyroxine and tr-iodo thyronine and certain blood biochemical constituents in growing crossbred (B. taurusXB. indicus) calves. Asian-Aust. J. Anim. Sci. 7:69-74

Singh, H. P., A. Kumar and P. C. Chaudhary. 1988. Veterinary Clinical Guide, 3rd edn. Kalyani Publishers, Ludhiana, India

Singh, J. L. and D. Swarup. 1994. Fluorosis in buffaloes. Veterinary Record. 135:260-261

Singh, J., B. Singh, M. Wadhwa and M. P. S. Bakshi. 2003. Effect of level of feeding on the performance of crossbred cows during pre and post-partum periods. Asian-Aust. J. Anim. Sci. 16:1749-1754

Singh, R. K., D. N. Verma and H. Q. Husain. 1994. Estimating the requirement of protein for maintenance and growth of female buffalo calves. Indian J. Anim. Nutr. 11:97-100

Skyes, A. R. and A. C. Field. 1973. Effects of dietary deficiencies of energy, protein and calcium on the pregnant ewe. N serum total protein, albumin, globulin transfer and plasma urea levels. J. Agric. Sci. 100:29-36

Snedecor, G. W. and W. G. Cochran. 1989. Statistical Methods. 9th edn. The Iowa State University Press, Ames, IA

Sun, S. and R. J. Christopherson. 2005. Urea kinetics in wethers exposed to different ambient temperatures at three dietary levels of crude protein. Asian-Aust. J. Anim. Sci. 18:795-801

Verma, D. N. 1998. Studies on protein requirement of buffalo calves. Buffalo Bulletin. 17:10-13

Webster, D. 1977. The immediate reaction between bromocresol green and serum as a measure of albumin content. Clin. Chem. 23:663-665