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Changes in Serum Biochemical Markers of Bone Cell Activity in Growing Thoroughbred Horses
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 Title & Authors
Changes in Serum Biochemical Markers of Bone Cell Activity in Growing Thoroughbred Horses
Inoue, Yoshinobu; Asai, Y.; Ohmori, H.; Fujii, H.; Matsui, T.; Yano, H.;
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We studied the changes in biochemical markers of bone metabolism in growing Thoroughbred horses. Serum osteocalcin (OC), as a marker for bone formation, and carboxy-terminal propeptide of type-I collagen (PICP), as a marker for bone formation, carboxy-terminal telopeptide of type-I collagen (ICTP), as a marker for bone resorption, were determined in nine clinically healthy horses from 3 d to 17 mo of age. The BW and withers height (WH) increased during the study. On the other hand, a rapid reduction in body weight gain (BWG) was observed between 1 mo and 9 mo of age and a rapid reduction in withers height gain was observed between 1 mo and 5 mo of age. The serum markers decreased significantly with increasing age. In particular, dramatic changes in serum markers occurred between 3 d to 1 wk and 5 to 7 mo of age in these horses, which suggests that bone turnover rapidly decreased after birth. On the other hand, the ratio of PICP to ICTP decreased through the experiment. This result suggests that the reduction in bone formation exceeded that of bone resorption. There was a significant correlation between markers and growth parameters, except for the correlation between PICP and BWG on single linear regression analysis. Serum OC and ICTP were affected by the WH in multiple linear regression analysis. These results indicated that the age-related variation in serum biochemical markers of bone metabolism reflected bone growth, but neither BW nor BWG. Therefore, we consider that changes in bone modeling are the major factor affecting the levels of serum biochemical markers by 17 mo of age in horses.
Horses;Biochemical Markers Of Bone Metabolism;Body Weight;Withers Height;Growth;
 Cited by
Allen, M. J. 2003. Biochemical markers of bone metabolism in animals: uses and limitations. Vet. Clin. Pathol. 32:101-113 crossref(new window)

Black, A., P. A. Schoknecht, S. L. Ralston and S. A. Shapses. 1999. Diurnal variation and age differences in the biochemical markers of bone turnover in horses. J. Anim. Sci. 77:75-83

Davicco, M. J., Y. Faulconnier, V. Coxam, H. Dubroeucq, W. Martin-Rosset and J. P. Barlet. 1994. Systemic bone growth factors in light breed mares and their foals. Arch. Int. Physiol. Biochim. Biophys. 102:115-119 crossref(new window)

Fraher, L. J. 1993. Biochemical markers of bone turnover. Clin.Biochem. 26:431-432 crossref(new window)

Hammett, F. S. 1925. A biochemical study of bone growth. I. Changes in the ash, organic matter, and water during growth (Mus Norvegicus Alubinus). J. Biol. Chem. 64:409-428

Hassager, C. and C. Christiansen. Influence of soft tissue body composition on bone mass and metabolism. Bone. 10:415-419 crossref(new window)

Hiney, K. 2004. Bone metabolism and activity. In Proceedings of the Texas A&M University conference on equine nutrition research, Collage Station, Texas, pp. 110-122

Kajantie, E., L. Dunkel, J. Risteli, M. Pohjavuori and S. Andersson. 2001. Markers of type I and III collagen turnover as indicators of growth velocity in very low birth weight infants. J. Clin. Endocr. Metab. 86:4299-4306 crossref(new window)

Lepage, O. M., M. Marcoux and A. Tremblay. 1990. Serum osteocalcin or bone Gla-protein, a biochemical marker for bone metabolism in horses: differences in serum levels with age. Can. J. Vet. Res. 54:223-226

Lepage, O. M., D. J. Hartmann, R. Eicher, B. Uebelhart, P. Tschudi and D. Uebelhart. 1998. Biochemical markers of bone metabolism in draught and warmblood horses. Vet. J. 156:169-175 crossref(new window)

Lepage, O. M., B. Carstanjen and D. Uebelhart. 2001. Noninvasive assessment of equine bone: an update. Vet. J. 161:10-22 crossref(new window)

Matsui, A., R. Katsuki, H. Fujikawa, M. Kai, K. Kubo, A. Hiraga and Y. Asai. 2004. Effects of uphill exercise on digestible energy intake and energy expenditure during exercise in yearling horses. Asian-Aust. J. Anim. Sci. 17:973-979 crossref(new window)

Melkko, J., S. Niemi, L. Risteli and J. Risteli. 1990. Radioimmunoassay of the carboxyterminal propeptide of human type I procollagen. Clin. Chem. 36:1328-1332

National Research Council. 1989. Nutrient requirements of horses. 5th Ed. Natinal Academy Press, Washington, DC

Ott, E. A. and J. Kivipelto. 2002. Influence of energy and protein content of the concentrate and restricting concentrate intake on growth and development of weanling horses. The Prof. Anim. Sci. 18:302-311

Parfitt, A. M., L. S. Simon, A. R. Villanueva and S. M. Krane. 1987. Procollagen type I carboxy-terminal extension peptide in serum as a marker of collagen biosynthesis in bone. Correlation with Iliac bone formation rates and comparison with total alkaline phosphatase. J. Bone Miner. Res. 2:427-436 crossref(new window)

Patterson-Allen, P., C. E. Brautigam, R. E. Grindeland, C. W. Asling and P. X. Callahan. 1982. A specific radioimmunoassay for osteocalcin with advantageous species crossreactivity. Anal. Biochem. 120:1-7 crossref(new window)

Price, J. S., B. Jackson, R. Eastell, A. E. Goodship, A. Blumsohn, L. Wright, S. Stoneham, L. E. Lanyon and R. G. Russell. 1995. Age related changes in biochemical markers of bone metabolism in horses. Equine. Vet. J. 27:201-207 crossref(new window)

Price, J. S. 1998. Biochemical markers of bone metabolism in horses: potentials and limitations? Vet. J. 156:163-165 crossref(new window)

Price, J. S., B. F. Jackson, J. A. Gray, P. A. Harris, L. M. Wright, D. U. Pfeiffer, S. P. Robins, R. Eastell and S. W. Ricketts. 2001. Biochemical markers of bone metabolism in growing thoroughbreds: a longitudinal study. Res. Vet. Sci. 71:37-44 crossref(new window)

Ravn, P., G. Cizza, N. H. Bjarnason, D. Thompson, M. Daley, R. D. Wasnich, M. McClung, D. Hosking, A. J. Yates and C. Christiansen. 1999. Low body mass index is an important risk factor for low bone mass and increased bone loss in early postmenopausal women. Early Postmenopausal Intervention Cohort (EPIC) study group. J. Bone Miner. Res. 14:1622-1627 crossref(new window)

Reller, E., J. Kivipelto and A. Ott. 2003. Age-related changes for serum bone metabolism markers in Thoroughbred and Quarter horse foals. J. Equine Vet. Sci. 23:117-120 crossref(new window)

Risteli, J., L. Elomaa, S. Niemi, A. Novamo and L. Risteli. 1993. Radioimmunoassay for the pyridinoline cross-linked carboxyterminal telopeptide of type I collagen: a new serum marker of bone collagen degradation. Clin. Chem. 39:635-640

SAS Institute Inc. 1990. SAS User's Guide. SAS Institute Inc., Cary, North Carolina

SAS Institute Inc. 1996. SAS/STAT software: Changes and enhancements through release 6.11. SAS Institute Inc., Cary, North Carolina

Stein, G. S., J. B. Lian and T. A. Owen. 1990. Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation. FASEB J. 4:3111-3123 crossref(new window)

Stover, S. M., R. R. Pool, R. B. Martin and J. P. Morgan. 1992. Histological features of the dorsal cortex of the third metacarpal bone mid-diaphysis during postnatal growth in thoroughbred horses. J. Anat. 181(Pt 3):455-69