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Influence of Osmolality of Complete Semen Extender on Motion Characteristics of Frozen-thawed Ram Spermatozoa
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 Title & Authors
Influence of Osmolality of Complete Semen Extender on Motion Characteristics of Frozen-thawed Ram Spermatozoa
Joshi, Anil; Mathur, A.K.; Naqvi, S.M.K.; Mittal, J.P.;
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 Abstract
The present study was conducted to observe the effect of osmolality of glycerolated TEST-yolk glycerol extenders on post-thawing sperm kinematics of ram spermatozoa of the native Malpura breed maintained in a semi-arid tropical environment. Good quality semen obtained from adult rams was pooled, split and diluted to 1,000 million spermatozoa per ml in complete TEST-yolk-glycerol extenders of 900, 1,200, 1,500 and 1,800 mOsm/kg osmolality. Diluted semen samples were loaded in 0.25 ml straws and cooled down to freezing temperature at the rate of per minute under controlled conditions before plunging into liquid nitrogen for storage. The thawing of straws was performed at in a water bath for 10 seconds and sperm kinematics of the frozen-thawed spermatozoa were assessed by a computer-assisted sperm analysis technique. Osmolality of diluent had no significant effect on post-thawing % motility, % rapid, % medium and % slow moving frozen-thawed spermatozoa but significantly (p< 0.05) affected the % linearity and % straightness. The post-thawing % motility and % rapid motile spermatozoa were highest in samples extended in diluent of 1,500 mOsm/kg osmolality and lowest in 900 mOsm/kg. The curvilinear velocity of spermatozoa was significantly (p<0.05) higher for samples extended in 1,800 mOsm/kg, compared to those in 900 and 1,200 mOsm/kg, but the effect was not significantly different to those extended in diluent of 1,500 mOsm/kg osmolality. The study indicated that ram spermatozoa could tolerate a wide osmolality range for dilution in the complete TEST-yolk-glycerol extender for their cryosurvival. The highest recovery of motile spermatozoa following thawing was achieved in samples extended in the TEST-yolk-glycerol diluent of 1,500 mOsm/kg osmolality.
 Keywords
Sheep;Extender;Osmolality;Spermatozoa;Cryopreservation;Computer-aided Semen Analysis;
 Language
English
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 References
1.
Aitken, R. J. 1990. Motility parameters and fertility (Ed. C. Gagnon). Controls of sperm motility: Biological and Clinical Aspects, CRC Press, Boca Raton, pp. 285-302

2.
Bag, S., A. Joshi, A. K. Mathur, P. S. Rawat and J. P. Mittal. 1998. Effect of thawing temperature on motion characteristics of frozen-thawed ram spermatozoa. Indian J. Anim. Sci. 69:16-18

3.
Bag, S., A. Joshi, A. K. Mathur, P. S. Rawat and J. P. Mittal. 1999. Effect of thawing temperature on motion characteristics of frozen-thawed ram spermatozoa. Indian J. Anim. Sci. 69:16-18

4.
Bag, S., A. Joshi, P. S. Rawat and J. P. Mittal. 2002a. Effect of initial freezing temperature on the semen characteristics of frozen-thawed ram spermatozoa in a semi-arid tropical environment. Small Rumin. Res. 43:23-29 crossref(new window)

5.
Bag, S., A. Joshi, S. M. K. Naqvi, P. S. Rawat and J. P. Mittal. 2002b. Effect of freezing temperature, at which straws were plunged into liquid nitrogen, on the post-thaw motility and acrosomal status of ram spermatozoa. Anim. Reprod. Sci. 72:175-183 crossref(new window)

6.
Bag, S., A. Joshi, S. M. K. Naqvi and J. P. Mittal. 2004. Effect of post-thaw incubation on sperm kinematics and acrosomal integrity of ram spermatozoa cryopreserved in medium-sized French straws. Theriogenol. 62:415-424 crossref(new window)

7.
Budworth, P. R., R. P. Amann and P. I. Chapman. 1988. Relationship between computerized measurements of motion of frozen-thawed bull spermatozoa and fertility. J. Androl. 9:41-54

8.
Davis, R. O. and D. F. Katz. 1992. Standardization and comparability of CASA instruments. J. Androl. 13:81-85

9.
Davis, R. O. and D. F. Katz. 1993. Operational standards for CASA instruments. J. Androl. 14:385-394

10.
Davis, R. O. and R. J.Siemers. 1995. Derivation and reliability of kinematic measures of sperm motion. Reprod. Fertil. Dev. 7:857-869 crossref(new window)

11.
Edward, A. Y., D. P. Windsor, I. W. Purvis, L. G. Sanchez-Partida and W. M. C. Maxwell. 1995. Distribution of variance associated with measurement of post-thaw function in ram sperm. Reprod. Fertil. Dev. 7:129-134 crossref(new window)

12.
Evans, G. and W. M. C. Maxwell. 1987. Handling and examination of semen. In: Salamon's artificial insemination of sheep and goats. Butterworths Pty Ltd., Australia, pp. 93-104

13.
Fiser, P. S., L. Ainsworth and G. A. Langford. 1981. Effect of osmolality of skim-milk diluents and thawing rate on cryosurvival of ram spermatozoa. Cryobiol. 18:399-403 crossref(new window)

14.
Fiser, P. S., L. Ainsworth and R. W. Fairfull. 1982. Cryosurvival of ram spermatozoa in hypertonic and isotonic diluents. Can. J. Anim. Sci. 62:425-428

15.
Fiser, P. S. and R. W. Fairfull. 1984. The effect of glycerol concentration and cooling velocity on cryosurvival of ram spermatozoa frozen in straws. Cryobiol. 21:542-551 crossref(new window)

16.
Fiser, P. S. and R. W. Fairfull. 1986. Combined effects of glycerol concentration, cooling velocity and osmolality of skim milk diluents on cryopreservation of ram spermatozoa. Theriogenol. 25:473-484 crossref(new window)

17.
Fiser, P. S. and R. W. Fairfull. 1989. The effect of glycerol-related osmotic changes on post-thaw motility and acrosomal integrity of ram spermatozoa. Cryobiol. 26:64-69 crossref(new window)

18.
Gillan,, L., W. M. C. Maxwell and G. Evans. 2004. Preservation and evaluation of semen for artificial insemination. Reprod. Fertil. Dev. 16:447-454 crossref(new window)

19.
Harvey, W. A. 1990. Mixed mode least squares and maximum likelihood computer programme. In: User's Guide for LSMLMW, Ohio State University, Columbus, USA

20.
Holt, W. V., M. F. Head and R. D. North. 1992. Freeze-induced membrane damage in ram spermatozoa is manifested after thawing: Observation with experimental cryomicroscopy. Biol. Reprod. 46:1086-1094 crossref(new window)

21.
Holt, W. V. and R. D. North. 1994. Effects of temperature and restoration of osmotic equilibrium during thawing on the induction of plasma membrane damage in cryopreserved ram spermatozoa. Biol. Reprod. 51:414-424 crossref(new window)

22.
Joshi, A., S. Bag and J. P. Mittal. 2000. Freezability of ram spermatozoa packaged in mini and medium size straws. Indian J. Anim. Prod. 32:25-26

23.
Joshi, A. and A. K. Mathur. 1996. Assessment of sperm kinematics of frozen-thawed ram spermatozoa. Indian J. Anim. Reprod. 17:42-44

24.
Joshi, A., S. Bag, S. M. K. Naqvi, R. C. Sharma, P. S. Rawat and J. P. Mittal. 2001. Effect of short-term and long-term preservation on motion characteristics of Garole ram spermatozoa: A prolific microsheep breed of India. Asian-Aust. J. Anim. Sci. 14:1527-1533

25.
Joshi, A., S. Bag, S. M. K. Naqvi, R. C. Sharma and J. P. Mittal. 2005. Effect of post-thawing incubation on sperm motility and acrosomal integrity of cryopreserved Garole ram semen. Small Rumin. Res. 56:231-238 crossref(new window)

26.
Kramer, C. Y. 1956. Extension of multiple range test to group means with unequal numbers of replicates. Biomet. 12:307- 310 crossref(new window)

27.
Maxwell, W. M. C. and P. F. Watson. 1996. Recent progress in the preservation of ram semen. Anim. Reprod. Sci. 42:55-65 crossref(new window)

28.
Pontbriand, D., J. G. Howard, M. C. Schiewe, L. D. Stuart and D. E. Wildt. 1989. Effect of cryoprotective diluent and method of freeze-thawing on survival and acrosomal integrity of ram spermatozoa. Cryobiol. 26:341-354 crossref(new window)

29.
Salamon, S. and W. M. C. Maxwell. 1995a. Frozen storage of ram semen. I. Processing, freezing, thawing and fertility after cervical insemination. Anim. Reprod. Sci. 37:189-249

30.
Salamon, S. and W. M. C. Maxwell. 1995b. Frozen storage of ram semen. II. Causes of low fertility after cervical insemination and methods of improvement. Anim. Reprod. Sci. 38:1-36 crossref(new window)

31.
Salamon, S. and W. M. C. Maxwell. 2000. Storage of ram semen. Anim. Reprod. Sci. 62:77-111 crossref(new window)

32.
Sanchez-Partida, L. G., B. P. Setchell and W. M. C. Maxwell. 1998. Effect of compatible solutes and diluent composition on the post-thaw motility of ram sperm. Reprod. Fertil. Dev. 10:347- 357 crossref(new window)

33.
Schmehl, M. K., S. P. Anderson, I. A. Vazques and E. F. Graham. 1986. The effect of dialysis of extended ram semen prior to freezing on post-thaw survival and fertility. Cryobiol. 23:406- 414 crossref(new window)

34.
Watson, P. F. 1995. Recent developments and concepts in the conservation of spermatozoa and the assessment of their postthawing function. Reprod. Fertil. Dev. 7:871-891 crossref(new window)

35.
Watson, P. F. 2000. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 60-61:481-492

36.
Yoshida, M. 2000. Conservation of sperms: current status and new trends. Anim. Reprod. Sci. 60-61:349-355

37.
Zhou, J. B., K. Z. Yue, M. J. Lou, Z. L. Chang, H. Liang, Z. Y. Wang and J. H. Tan. 2004. Effect of extenders and temperatures on sperm viability and fertilizing capacity of Harbin White boar semen during long-term liquid storage. Asian-Aust. J. Anim. Sci. 17:1501-1508