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Influence of Rumen Escape Starch on α-Amylase Activity in Pancreatic Tissue and Small Intestinal Digesta of Lambs
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 Title & Authors
Influence of Rumen Escape Starch on α-Amylase Activity in Pancreatic Tissue and Small Intestinal Digesta of Lambs
Xu, M.; Yao, J.H.; Wang, Y.H.; Wang, F.N.;
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 Abstract
Two slaughter experiments were conducted to determine the effects of rumen escape starch, by altering dietary starch concentration and corn particle size, on -amylase activity in the pancreas and the small intestinal digesta of lambs. In experiment 1, 18 wether lambs (28.51.6 kg) were fed low, medium or high starch diets for 35 d and slaughtered. Dietary starch concentrations linearly increased rumen escape starch (p<0.05). Pancreatic -amylase activity was lower (p<0.05) in lambs fed the low starch diet. When expressed per gram of digesta, -amylase activity was lower in lambs fed the low starch diet. However, expressed as total activity, -amylase in the digesta was greater in lambs fed the medium starch diet. In experiment 2, 12 wether lambs (23.50.3 kg) were fed diets with finely cracked corn, coarsely cracked corn and whole corn. These dietary treatments continued for 35 d before tissue collection. Rumen escape starch increased with increasing corn particle size (p<0.05). -amylase activity in the pancreas and the small intestinal digesta was significantly greater (p<0.05) in lambs fed the coarsely cracked corn. These data suggest that increasing rumen escape starch results in a quadratic increase in total -amylase activity in the pancreas and the small intestinal digesta. Maximum -amylase activity is reached when rumen escape starch is about 100-120 g/d in 25-30 kg lambs.
 Keywords
Starch;a-Amylase;Pancreas;Digesta;Lamb;
 Language
English
 Cited by
1.
Effects of Different Levels of Supplementary Alpha-amylase on Digestive Enzyme Activities and Pancreatic Amylase mRNA Expression of Young Broilers,;;;;;

Asian-Australasian Journal of Animal Sciences, 2008. vol.21. 1, pp.97-102 crossref(new window)
 References
1.
AOAC. 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Arlington, Virginia

2.
Bauer, M. L., D. L. Harmon, D. W. Bohnert, A. F. Branco and G. B. Huntington. 2001. Influence of (alpha)-linked glucose on sodium-glucose cotransport activity along the small intestine in cattle. J. Anim. Sci. 79:1917-1924

3.
Call, J. L., G. E. Mitchell, Jr. and C. O. Little. 1975. Response of ovine pancreatic amylase to elevated blood glucose. J. Anim. Sci. 41:1717-1721

4.
Chittenden, L. W., D. D. Johnson, G. E. Mitchell, Jr. and R. E. Tucker. 1984. Ovine pancreatic amylase response to form of carbohydrate. Nutr. Rep. Int. 29:1051-1060

5.
Clary, J. J., G. E. Mitchell, Jr, C. O. Little and N. W. Bradley. 1969. Pancreatic amylase activity from ruminants fed different rations. Can. J. Physiol. Pharmacol. 47:161-164

6.
Elizalde, J. C., N. R. Merchen and D. B. Faulkner. 1999. Supplemental Cracked Corn for Steers Fed Fresh Alfalfa: I. Effects on Digestion of Organic Matter, Fiber, and Starch. J. Anim. Sci. 77:457-466

7.
Harmon, D. L., R. M. Yamka and N. A. Elam. 2004. Factors affecting intestinal starch digestion in ruminants: a review. Can. J. Anim. Sci. 84:309-318

8.
Herrera-Saldana, R. and J. T. Huber. 1989. Influence of varying protein and starch degradabilities on performance of lactating cows. J. Dairy Sci. 72:1477-1483

9.
Janes, A. N., T. E. C. Weeks and D. G. Armstrong. 1985. Carbohydrase activity in the pancreatic tissue and small intestine mucosa of sheep fed dried-grass or ground maizebased diets. J. Agric. Sci. (Camb.) 104:435-443

10.
Jang, I. S., Y. H. Ko, H. Y. Yang, J. S. Ha, J. Y. Kim, S. Y. Kang, D. H. Yoo, D. S. Nam, D. H. Kim and C. Y. Lee. 2004. Influence of essential oil components on growth performance and the functional activity of the pancreas and small intestine in broiler chickens. Asian-Aust. J. Anim. Sci. 17:394-400

11.
Karr, M. R., C. O. Little and G. E. Mitchell. 1966. Starch disappearance from different segments of the digestive tract of steers. J. Anim. Sci. 25:652-654

12.
Knowlton, K. F., M. S. Allen and P. S. Erickson. 1996. Lasaloid and particle size of corn grain for dairy cows in early lactation: 2. Effect on Ruminal Measurements and Feeding Behavior. J. Dairy Sci. 79:565-574

13.
Kreikemeier, K. K., D. L. Harmon, J. P. Peters, K. L. Gross, C. K. Armendariz and C. R. Krehbiel. 1990. Influence of dietary forage and feed intake on carbohydrase activities and small intestinal morphology of calves. J. Anim. Sci. 68:2916-2929

14.
Kreikemeier, K. K., D. L. Harmon, R. T. Brandt, Jr, T. B. Avery and D. E. Johnson. 1991. Small intestinal starch digestion in steers: effect of various levels of abomasal glucose, corn starch and corn dextrin infusion on small intestinal disappearance and net glucose absorption. J. Anim. Sci. 69:328-338

15.
Li, F. C., Y. L. Feng, Z. H. Wang and H. Lin. 2000. Effects of varying levels of flaked corn starch abomasally infused on nitrogen retention and the efficiency of energy conversion in fattening steers. Asian-Aust. J. Anim. Sci. 13:470-473

16.
Liu, Y. Q., B. S. Wang, Y. J. Zhang, E. Y. Zhen and H. X. Sun. 2004. Effect of diets on digestive enzymes activity of small intestine in lambs. China Herbivores (China). 24(S1):131-135

17.
National Research Council. 2001. Nutrient requirements of dairy cattle. 7th edition Washington, DC: National Academy Press

18.
Owens, F. N., R. A. Zinn and Y. K. Kim. 1986. Limits to starch digestion in the ruminant small intestine. J. Anim. Sci. 63:1634-1648

19.
Poore, M. H., J. A. Moore, T. P. Eck, R. S. Swingle and W. H. Brown. 1991. Wheat straw or alfalfa hay in diets with 30% neutral detergent fiber for lactating Holstein cows. J. Dairy Sci. 74:3152-3159

20.
Rémond, D., J. I. Cabrera-Estrada, L. M. Champion, B. Chauveau, R. Coudure and C. Poncet. 2004. Effect of Corn Particle Size on Site and Extent of Starch Digestion in Lactating Dairy Cows J. Dairy Sci. 87:1389-1399

21.
Richards, C. J., K. C. Swanson, S. J. Paton, D. L. Harmon and G. B. Huntington. 2003. Pancreatic exocrine secretion in steers infused postruminally with casein and cornstarch. J. Anim. Sci. 81:1051-1056

22.
Russell, J. R., A. W. Young and N. A. Jorgensen. 1981. Effect of dietary corn starch intake on pancreatic amylase and intestinal maltase and pH in cattle. J. Anim. Sci. 52:1177-1182

23.
Swanson, K. C., J. C. Matthews, A. D. Matthews, J. A. Howell, C. J. Richards and D. L. Harmon. 2000. Dietary carbohydrate source and energy intake influence the expression of pancreatic alpha-amylase in lambs. J. Nutr. 130:2157-2165

24.
Swanson, K. C., J. C. Matthews, C. A. Woods and D. L. Harmon. 2002a. Postruminal administration of partially hydrolyzed starch and casein influences pancreatic $\alpha$-amylase expression in calves. J. Nutr. 132:376-381

25.
Swanson, K. C., C. J. Richards and D. L. Harmon. 2002b. Influence of abomasal infusion of glucose or partially hydrolyzed starch on pancreatic exocrine secretion in beef steers. J. Anim. Sci. 80:1112-1116

26.
Swanson, K. C., J. A. Benson, J. C. Matthews and D. L. Harmon. 2004. Pancreatic exocrine secretion and plasma concentration of some gastrintestine hormones in response to abomasal infusion of starch hydrolyzed and/or casein. J. Anim. Sci. 82:1781-1787

27.
Swanson, K. C., J. C. Matthews, C. A. Woods and D. L. Harmon. 2003. Influence of substrate and/or neurohormonal mimic on in vitro pancreatic enzyme release from calves postruminally infused with partially hydrolyzed starch and/or casein. J. Anim. Sci. 81:1323-1331

28.
Walker, J. A. and D. L. Harmon. 1995. Influence of ruminal or abomasal starch hydrolysate infusion on pancreatic exocrine secretion and blood glucose and insulin concentrations in steers. J. Anim. Sci. 73:3766-3774

29.
Walker, J. A. and D. L. Harmon. 1996. Technical note: a simple, rapid assay for $\alpha$-amylase in bovine pancreatic juice. J. Anim. Sci. 74:658-662

30.
Wang, X. B., T. Ogawa, S. Suda, K. Taniguchi, H. Uike, H. Kumagai and K. Mitani. 1998. Effects of nutritional level on digestive digestive enzyme in the pancreas and small intestine of calves slaughtered at same body weight. Asian-Aust. J. Anim. Sci. 11:375-3802

31.
Wang, X. B., K. Taniguchi and T. Obitsu. 2000. Response of Pancreatic Exocrine Secretion in Sheep Fed Different Type and Amount of Hay. Asian-Aust. J. Anim. Sci. 13:1044-1048

32.
Wang, X. B. and K. Taniguchi. 1998. Activity of pancreatic digestive enzyme in sheep given abomasal infusion of starch and casein. Anim. Sci. Technol (Japan). 69:870-874

33.
Yu, P., J. T. Huber, F. A. P. Santos, J. M. Simas and C. B. Theurer. 1998. Effects of Ground, Steam-Flaked, and Steam-Rolled Corn Grains on Performance of Lactating Cows. J. Dairy Sci. 81:777-783

34.
Zhang, Y. J., Y. Q. Liu, H. X. Sun, E. Y. Zhen, B. S. Wang and Y. Z. Liu. 2005. Study on the development of digesta pH and main digestive enzymes of small intestine in lamb. Acta Veterinaria Et Zootechnica Sinica (China). 36:149-152