Advanced SearchSearch Tips
Influence of Transgenic Corn on the In vitro Rumen Microbial Fermentation
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Influence of Transgenic Corn on the In vitro Rumen Microbial Fermentation
Sung, Ha Guyn; Min, Dong Myung; Kim, Dong Kyun; Li, De Yun; Kim, Hyun Jin; Upadhaya, Santi Devi; Ha, J.K.;
  PDF(new window)
In this study, the comparative effects of transgenic corn (Mon 810 and Event 176) and isogenic corn (DK729) were investigated for their influence on in vitro rumen fermentation. This study consisted of three treatments with 0.25 g rice straw, 0.25 g of corn (Mon810/Event176/DK 729) mixed with 30 ml rumen fluid-basal medium in a serum bottle. They were prepared in oxygen free conditions and incubated at in a shaking incubator. The influence of transgenic corn on the number of bacterial population, F. succinogenes (cellulolytic) and S. bovis (amylolytic), was quantified using RT-PCR. Fermentative parameters were measured at 0, 2, 4, 8, 12 and 24 h and substrate digestibility was measured at 12 and 24 h. No significant differences were observed in digestibility of dry matter, NDF, ADF at 12 and 24 h for both transgenic and isogenic form of corns (p>0.05) as well as in fermentative parameters. Fluid pH remained unaffected by hybrid trait and decreased with VFA accumulation as incubation time progressed. No influence of corn trait itself was seen on concentration of total VFA, acetic, propionic, butyric and valeric acids. There were no significant differences (p<0.05) in total gas production, composition of gas (methane and hydrogen) at all times of sampling, as well as in NH3-N production. Bacterial quantification using RT-PCR showed that the population number was not affected by transgenic corn. From this study it is concluded that transgenic corn (Mon810 and Event 176) had no adverse effects on rumen fermentation and digestibility compared to isogenic corn. However, regular monitoring of these transgenic feeds is needed by present day researchers to enable consumers with the option to select their preferred food source for animal or human consumption.
Transgenic Corns;GMO;Mon810;Event176;Rumen;
 Cited by
Low Ruminal pH Reduces Dietary Fiber Digestion via Reduced Microbial Attachment,Sung, Ha Guyn;Kobayashi, Yasuo;Chang, Jongsoo;Ha, Ahnul;Hwang, Il Hwan;Ha, J.K.;

Asian-Australasian Journal of Animal Sciences, 2007. vol.20. 2, pp.200-207 crossref(new window)
Beever, D. E., K. Glenn and R. H. Phipps. 2003. A safety evaluation of genetically modified feedstuffs for livestock production; the fate of transgenic DNA and proteins. Asian- Aust. J. Anim. Sci. 16:764-772

Brake, J. and D. Vlachos. 1998. Evaluation of transgenic event 176 "Bt" corn in broiler chickens. Poult. Sci. 77:648-653

Chiueh, L. C., Y. L. Chen, J. H. Yu and D. Y. C. Shin. 2001. Detection of four types of genetically modified maize by polymerase chain reaction and immuno-Kit methods. J. Food and Drug Anal. 9:50-57

Clark, J. H. and I. R. Ipharraguerre. 2001. Livestock performance: Feeding biotech crops. J. Dairy Sci. 84(E. Suppl):E9-E18

Donkin, S. S., J. C. Velez, A. K. Totten, E. P. Stanisiewski and G. F. Hartnell. 2003. Effects of feeding silage and grain from glyphosate-tolerant or insert-protected corn hybrids on feed intake, ruminal digestion, and milk production in dairy cattle. J. Dairy Sci. 86:1780-1788

Einspanier, R., B. Lutz, R. Stefanie, O. Berezina, V. Zverlov, W. Schwarz and J. Mayer. 2004. Tracing residual recombinant feed molecules during digestion and rumen bacterial diversity in cattle fed transgenic maize. Eur. Food Res. Technol. 218:269-273 crossref(new window)

Faust, M. A. 1999. Research update on Bt on silage. In: Four-state Applied Nutrition and Magement Conf., Midwest Plan service, Ames, IA, USA. pp. 157-164

Folmer, J. D., R. J. Grant, C. T. Milton and J. Beck. 2002. Utilization of Bt corn residues by grazing beef steers and Bt corn silage and grain by growing beef cattle and lactating dairy cows. J. Anim. Sci. 80:1352-1361

James, C. 2005. Preview: Global Status of Commercialized Biotech/GM Crops: 2004. ISAAA Briefs No. 32. Ithaca, NY

Kim, C. H., J. K. Park, G. Y. Lee and I. J. Seo. 2005. In vitro fermentation of rumen mictoorganisms culture in medium supplemented with bactreio-mineral water (BMW) producrd from bio-reacted swine manure. Asian-Aust. J. Anim. Sci. 18(10):1435-1439

Koziel, M. G., G. L. Beland, C. Bowman, N. B. Carozzi, R. Crensgaw, L. Crossland, J. Dawson, N. Desai, M. Hill, S. Kadwell, K. Launis, K. Lewis, D. Maddox, K. McOherson, M. R. Meghji, E. Merlin, R. Rhodes, G. W. Warren, M. Wright and S. V. Evola. 1993. Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Biotechnol. 11:194-200 crossref(new window)

Kumar, R. and R. S. Dass. 2005. Effect of niacin supplementation on rumen metabolites in Murrah buffaloes (Bubalus bubalis). Asian-Aust. J. Anim. Sci. 18:38-41

Kuribara, H., Y. Shindo, T. Matsuoka, T. Takubo, K. Futo, S. Aoki, N. Hirao, T. Akiyama, H. Goda, Y. Toyoda and A. Hino. 2002. Novel reference molecules for quantitation of genetically modified maize and soybean. J. AOAC Int. 85:1077-1089

Masoero, F., M. Moschini, F. Rossi, A. Prandoni and A. Pietri. 1999. Nutritive value, mycotoxin contamination and in vitro rumen fermentation of normal and generically modified corn (cry1A(B)) grown in northern Italy. Maydica. 44:205-209

LeBrun, M., A. Sailland and G. Freyssienet. 1997. Mutated 5- enolpyruvyl-shikimate-3-phosphate synthase, genee cording of said protein and transformed plants containing said gene. Int. Pat. Appl. WO 97/04103

Lee, S. H., Y. H. Park, J. K. Kim, K. W. Park and Y. M. Kim. 2004. Qualitative PCR method for detection of genetically modified maize lines NK603 and TC1507. Agric. Chem. Biotechnol. 47:185-188

Lee, S. H., D. M. Min and J. K. Kim. 2006. Qualitative and quantitative polymerase chain reaction analysis for genetically modified maize MON863. J. Agric. Food Chem. 54:1124-1129 crossref(new window)

McDougall, E. I. 1948. Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochem. J. 43:99- 109

Ministry of Agriculture and Forestry of Korea. 2000. Notification No. 2000-31(April 22, 2000)

Ministry of Agriculture, Forestry and Fisheries in Japan. 2000. Food and Marketing Burea, Notification No. 1775 (Jane 10, 2000)

Phipps, R. H., D. E. Beever and D. J. Humphries. 2002. Detection of transgenic DNA in milk from cows receiving herbicide tolerant (CP4EPSPS) soyabean meal. Livestock Prod. Sci. 74:269-273 crossref(new window)

Phipps, R. H., A. K. Joes, A. P. Tingey and S. Abeyasekera. 2005. Effect of corn silage from an herbicide-tolerant genetically modified variety on milk production and absence of transgenic DNA in milk. J. Dairy Sci. 88:2870-2878

Purdy, K. J., T. M. Embley, S. Takii and D. B. Nedwell. 1996. Rapid extraction of DNA and RNA from sediments by novel hydroxyapatite spin-colum method. Appl. Environ. Microbial. 62:3905-3970

Saxena, D. and G. Stotzky. 2001. Bt corn has higher lignin content than non-Bt corn. Am. J. Botany. 88:1704-1706 crossref(new window)

Sidhu, R. S., B. G. Hammond, R. L. Fuchs, J. Mutz, L. R. Holden, B. George and T. Olson. 2000. Glyphosate-tolerant corn: The composition and feeding value of grain from glyphosatetolerant corn is equivalent to that of conventional corn (Zea mays L.) J. Agric. Food Chem. 48:2305-2312 crossref(new window)

Steel, R. G. D. and J. H. Torrie. 1986. Principles and Procedure of Statistics: A Biometrical Approach 2nd ed MCGraw Hill Book Co., Inc., New York

Steinrucken, H. C. and N. Amrhein. 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimate-3- phosphate sythase. Biochem. Biophys. Res. Commun. 94:1207-1212 crossref(new window)

Tajima, K., R. I. Aminov, T. Nagamine, H. Matsui, M. Nakamura and Y. Benno. 2001. Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Appl. Environ. Microbiol. 67:2766-2774 crossref(new window)

The European Parliament and the Council of the European Union, 2003. Regulation (EC) No.1829/2003. Office J. Euro Uni. Sec2. Article 24

Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods of dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597

Wang, J. H., S. H. Choi, C. G. Yan and M. K. Song. 2005. Effect of monensin and fish oil supplementation on biohydration and CLA production by rumen bacteria in vitro when incubated with safflower oil. Asian-Aust. J. Anim. Sci. 18:221-225

Yonemochi, C., T. Ikeda, C. Harada, T. Kusama and M. Hanazum. 2003. Influence of transgenic (CBH 351, named Starlink) on health condition of dairy cow and transfer of cry9c protein and cry9c gene to milk, blood, liver and muscle. Anim. Sci. J. 74:81-88 crossref(new window)