Advanced SearchSearch Tips
Characterization of Lactic Bacterial Strains Isolated from Raw Milk
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Characterization of Lactic Bacterial Strains Isolated from Raw Milk
Kim, Hyun-jue; Shin, Han-seung; Ha, Woel-kyu; Yang, Hee-jin; Lee, Soo-won;
  PDF(new window)
During lactic acid bacteria (LAB) transit through the gastrointestinal tract, ingested microorganisms were exposed to successive stress factors, including low pH in the human stomach and in bile acid. These stress factors can be used as criteria for the selection of a viable probiotic strain. Four such strains (Lactobacillus helveticus SGU 0011, Lactobacillus pentosus SGU 0010, Streptococcus thermophilus SGU 0021 and Lactobacillus casei SGU 0020) were isolated from raw milk. When the identified LAB were exposed to synthetic gastric juice, whereas L. casei SGU 0020 and S. thermophilus SGU 0021 exhibited a 0% survival rate, L. helveticus SGU 0011 and L. pentosus SGU 0010 exhibited 60% and 95% survival rates. L. casei SGU 0020 and S. thermophilus SGU 0021 could not be examined with regard to their tolerances to artificial bile juice, as they uniformly died upon exposure. However, L. helveticus SGU 0011 and L. pentosus SGU 0010 individually survived at rates of 39% and 93%. Also, all four of these strains were confirmed to be tolerant of ten different antibiotics.
Lactobacillus;Probiotics;Artificial Gastric Juice;rDNA;Antibiotic;
 Cited by
β-Galactosidase from Lactobacillus pentosus: Purification, characterization and formation of galacto-oligosaccharides, Biotechnology Journal, 2010, 5, 8, 838  crossref(new windwow)
Brandt, L. J. and L. H. 1976. Bernstein. Bile salts: their role in cholesterol synthesis, secretion and lithogenesis. Am. J. Gastroenterol. 65:17-30

Dunne, C., L. O'Mahony, L. Murphy, G. Thornton, D. Morrissey, S. O'Halloran, M. Feeney, S. Flynn, G. Fitzgerald, C. Daly, B. Kiely, G. C. O'Sullivan, F. Shanahan and J. K. Collins. 2001. In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am. J. Clin. Nutr. 73:386S- 392S crossref(new window)

Floch, M. H., H. J. Binder, B. Filburn and W. Gershengoren. 1972. The effect of bile acids on intestinal microflora. Am. J. Clin. Nutr. 25:1418-1426 crossref(new window)

Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66:365-378 crossref(new window)

Gilliland, S. E. 1979. Beneficial interrelationships between certain microoganisms and humans: candidate microoganism for use as dietary adjuncts. J. Food Prot. 42:164-167 crossref(new window)

Havenaar, R., B. T. Brink and J. H. J. Huis isn't Veld. 1992. Selection of strains for probiotic use. pp. 209-224 in probiotics. (Ed. R. Fuller). Chapman & Hall. London

Henriksson, A., A. K. D. Khaled and P. L. Conway. 1999. Lactobacillus colonization of the gastrointestinal tract of mice after removal of the nonsecreting stomach region. Microb. Ecol. Health Dis. 11:96-99 crossref(new window)

Holt, J. G., N. R. Krieg, P. H. A. Sneath, J. T. Staley and S. T. Williams. 1994. In: Bergey's Manual of Determinative Bacteriology, 9th Ed. (Ed. W. R. Hensyl), pp. 527-566. Williams and Wilkins, Baltimore, MD

Hood, S. K. and E. A. Zottola. 1988. Effects of low pH on the ability of Lactobacillus acidophillus to survive and adhere to human intestinal cells. J. Food Sci. 53:1514-1516 crossref(new window)

Ingrid, S. Surono. 2003. In vitro probiotic properties of indigenous dadih lactic acid bacteria. Asian-Aust. J. Anim. Sci. 16(5):726- 731 crossref(new window)

Kobayashi, Y., K. Toyama and T. Terashima. 1974. [Biological characteristics of Lactobacillus. II. Tolerance of a multiple antibiotic resistant strain, Lactobacillus casei PSR 3002, to artificial digestive fluids] Nippon Saikingaku Zasshi. 29:691- 697 crossref(new window)

Lilly, D. M. and R. H. Stillwell. 1965. Probiotics: growthpromoting factors produced by microorganisms. Sci. 147:747- 748 crossref(new window)

Marteau, P., P. pochart, Y. Bouhnik and J. C. Rambaud. 1993. Fate and effects of some transiting microoganism in the human gastrointestinal tract. World Reu. Nutr. Diet. 74:1

Oh, S., S. H. Kim and R. W. Worobo. 2000. Characterization and purification of a bacteriocin produced by a potential probiotic culture, Lactobacillus acidophilus 30SC. J. Dairy Sci. 83:2747- 2752 crossref(new window)

Pedersen, C., H. Jonsson, J. E. Lindberg and S. Roos. 2004. Microbiological characterization of wet wheat distillers' grain, with focus on isolation of lactobacilli with potential as probiotics. Appl. Environ. Microbiol. 70:1522-1527 crossref(new window)

Renner, E. 1991. Cultured dairy products in human nutrition. Bull. Int. Dairy Fed. 255:2-24

Robins-Browne, R. M. and M. M. Levine. 1981. The fate of ingested Lactobacilli in the proximal small intestine. Am. J. Clin. Nutr. 34:514-519 crossref(new window)

Ronka, E., E. Malinen, M. Sarela, M. Rinta-Koski, J. Aarnikunnas and A. Palva. 2003. Probiotic and milk technological properties of Lactobacillus brevis. Int. J. Food Microbiol. 83:63-74 crossref(new window)

Simon, G. L. and S. L. Gorbach. 1987. Intestinal flora and gastrointestinal function. pp. 1729 in Physiology of the gastrointestinal tract. Vol. 2. 2nd (Ed. L. R. Johnson), ed Raven Press, New York

Stewart, L., C. A. Pellegrini and L. W. Way. 1986. Antibacterial activity of bile acids against common biliary tract organism. Surg Forum. 37:157-159

Usman Pato. 2003. Bile and acid tolerance of lactic acid bacteria isolated from dadih and their antimutagenicity against mutagenic heated tauco. Asian-Aust. J. Anim. Sci. 16(11):1680-1685 crossref(new window)