JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Genetic Variation of Growth Hormone Gene and Its Relationship with Milk Production Traits in China Holstein Cows
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Genetic Variation of Growth Hormone Gene and Its Relationship with Milk Production Traits in China Holstein Cows
Zhou, Guo-li; Zhu, Qi; Jin, Hai-guo; Guo, Shan-li;
  PDF(new window)
 Abstract
Associations were analysed between polymorphisms localized in intron 3 of the growth hormone gene (GH-MspI) and milk production traits of 543 China Holstein cows. A PCR-RFLP method was used for identification of genotypes. The following frequencies of genotypes and alleles were found: 0.77, 0.21 and 0.02 for +/+, +/- and -/-, respectively, and 0.87 and 0.13 for and , respectively. Significant differences between herds were observed in the frequencies of both genotypes and alleles. The results of least squares analysis showed that in all three lactation phases the GH +/+ cows yielded most milk (p<0.01 for lactation I and p<0.05 for lactations II and III), whereas +/- cows showed higher milk fat content than +/+ individuals (p<0.05 for lactation I and II, and p<0.01 for lactation III). The +/+ cows yielded more fat than +/- individuals (p<0.01 only in lactation I). The +/+ cows yielded more milk protein than +/- individuals (p<0.01 for lactation I, II, and III). The +/+ cows produced milk of higher protein content than that of +/- individuals (p<0.05 only in lactation II). Based on these results, we conclude that the +/+ of GH locus should be the favored genotype in China Holstein cow breeds for use in marker-assisted selection programmes.
 Keywords
China Holstein Cows;Growth Hormone Gene;PCR-RFLP;Genetic Polymorphism;Milk Production Traits;
 Language
English
 Cited by
 References
1.
Aruna Pal, A., K. Chakravarty, T. K. Bhattacharya, B. K. Joshi and Arjava Sharma. 2004. Detection of Polymorphism of Growth Hormone Gene for the Analysis of Relationship between Allele Type and Growth Traits in Karan Fries Cattle. Asian-Aust. J. Anim. Sci. 17:1334-1337

2.
Biswas, T. K., T. K. Bhattacharya, A. D. Narayan, S. Badola, Pushpendra Kumar and Arjava Sharma. 2003. Growth Hormone Gene Polymorphism and Its Effect on Birth Weight in Cattle and Buffalo. Asian-Aust. J. Anim. Sci. 16:494-497

3.
Cowan, C. M., M. R. Dentine, R. L. Ax and L. A. Schuler. 1989. Restriction fragment length polymorphism associated with growth hormone and prolactin genes in Holstein bulls: evidence for a novel growth hormone allele. Anim. Genet. 20:157-165

4.
Chrenek, P., J. Huba, D. Vasicek, D. Peskovicova and J. Bulla. 2003. The Relation between Genetic Polymorphism Markers and Milk Yield in Brown Swiss Cattle Imported to Slovakia. Asian-Aust. J. Anim. Sci. 16:1397-1401

5.
Falaki, M., N. Gengler, M. Sneyers, A. Prandi, S. Massart, A. Formigoni, A. Burny, D. Portetelle and R. Renaville. 1996. Relationships of polymorphism for growth hormone and growth hormone receptor genes with milk production traits for Italian Holstein-Friesian bulls. J. Dairy Sci. 79:1446-1453

6.
Gordon, D. F., D. P. Quick, C. R. Ewin, J. E. Donelson and R. A. Maurer. 1983. Nucleotede sequence of the bovine growth hormone chromosomal gene. Mol. Cell. Endocrinol. 33:81-95 crossref(new window)

7.
Hediger, R., S. E. Johnson, W. Barendse, R. D. Drinkwater, S. S. Moore and J. Hetzel. 1990. Assignment of the growth hormone gene locus to 19q26-qter in cattle and to 11q25-qter in sheep by in situ hybridization. Genomics. 8:171-174 crossref(new window)

8.
Hilbert, P., A. Marcotte, A. Schwers, R. Hanset, G. Vassart and M. Georgens. 1989. Aanalysis of genetic variation in the Belgian Blue Cattle bredd using DNA sequence polymorphism at the growth hormone, low density lipoprotein receptor, ${\alpha}$-subunit of glycoprotein hormones and thyroglobin loci. Anim. Genet. 20:383-394

9.
Hoj, S., M. Fredholm, N. J. Larsen and V. H. Nielsen. 1993. Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle. Anim. Genet. 24:91- 96

10.
Lagziel, A., S. Denise, O. Hanotte, S. Dhara, V. Glazko, A. Broadhead, R. Davoli, V. Russo and M. Soller. 2000. Geographic and breed distribution of an Msp I PCR-RFLP in the bovine growth homone (bGH) gene. Anim. Genet. 31:210- 213 crossref(new window)

11.
Lagziel, A., E. Lipkin, E. Ezra, M. Soller and J. I. Weller. 1999. An Msp I polymorphism at the bovine growth hormone (bGH) gene is linked to a locus affecting milk protein percentage. Anim. Genet. 30:296-299 crossref(new window)

12.
Lagziel, A., E. Lipkin and M. Soller. 1996. Association between SSCP haplotypes at the bovine growth hormone gene and milk protein percentage. Genet. 142:945-951

13.
Lee, B. K., G. F. Lin, B. A. Crooker, M. P. Murtaugh, L. B. Hansen and H. Chester-Jones. 1993. Association of somatotropin (bST) gene polymorphism with selection for milk yield in Holstein cows. J. Dairy Sci. 76:149

14.
Lingappa, V. R., A. Ddevillers-Thiery and G. Blobel. 1977. Nascent prehormones are intermediates in the biosynthesis of authentic bovine pituitary growth hormone and prolactin. Proc. Natl. Acad. Sci. USA. 74:2432-2436

15.
Mttra, A., P. Schlee, C. R. Balakrishnan and F. Pirchner. 1995. Polymorphisms at growth hormone and prolactin loci in Indian cattle and buffalo. J. Anim. Breed. Genet. 112:71-74

16.
Sabour, M. P., C. Y. Lin and C. Smtth. 1997. Association of genetic variants of bovine growth hormone with milk production traits in Holstein cattle. J. Anim. Breed. Genet. 114:435-442

17.
Sambrook, J. 1989. Molecular cloning: a laboratory manual, 2nd edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

18.
Seavey, B. K., R. N. P. Singh, U. J. Lewis and I. I. Geschwind. 1971. Bovine growth hormone: evidence for two allelic forms. Biochem. Bioph. Res. Co. 43:189-195 crossref(new window)

19.
Vukasinovic, N., S. K. Denise and A. E. Freeman. 1999. Association of growth hormone loci with milk yield traits in Holstein bulls. J. Dairy Sci. 82:788-794

20.
Wallis, M. 1973. The primary structure of bovine growth hormone. FEBS Lett. 35:11-14 crossref(new window)

21.
Wang, W. J., L. S. Huang, J. Gao, N. S. Ding, K. F. Chen, J. Ren and M. Luo. 2003. Polymorphism of Growth Hormone Gene in 12 Pig Breeds and Its Relationship with Pig Growth and Carcass Traits. Asian-Aust. J. Anim. Sci. 16:161-164

22.
Woychick, R. P., S. A. Camper, R. H. Lyons, S. Horowtts, E. C. Goodwin and F. M. Rottman. 1982. Cloning and nucleotide sequencing of the bovine growth hormone gene. Nucl. Acids Res. 10:7197-7210 crossref(new window)

23.
Yao, J., S. E. Aggerey, D. Zadworny, J. E. Hayes and U. Kuhnlein. 1996. Sequence variations in the bovine growth hormone gene characterized by single strand conformation polymorphism (SSCP) analysis and their association with milk production traits in Holstein. Genet. 144:1809-1816

24.
Zhang, H. M., K. C. Maddock, D. R. Brown, S. K. Denise and R. L. AX. 1993. A novel allele of the bovine somatotropin gene detected by PCR-RFLP analysis. J. Anim. Sci. 71:2276