JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effect of Dietary Vitamin E Supplementation on Serum α-Tocopherol and Immune Status of Crossbred Calves
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Dietary Vitamin E Supplementation on Serum α-Tocopherol and Immune Status of Crossbred Calves
Samanta, A.K.; Dass, R.S.; Rawat, Mayank; Mishra, S.C.; Mehra, U.R.;
  PDF(new window)
 Abstract
An experiment was conducted with twenty crossbred male calves (7-15 days old) divided into 4 different experimental groups on the basis of body weights to study the effect of vitamin E supplementation on the serum -tocopherol concentration and immune response of the calves. All the calves were fed on milk and calf starter up to 13 weeks and afterwards, they were fed on concentrate mixture and oat hay up to 32 weeks of age. In addition, the calves in groups I, II, III and IV were supplemented with 0, 125, 250 and 500 IU feed grade DL--tocopheryl acetate, respectively. Blood samples were collected at 0 day and subsequently at 1, 2, 3, 4, 6 and 8 months of age to monitor the serum -tocopherol concentration in crossbred calves. After 24 weeks of experimental feeding, 4 animals from each group were intramuscularly inoculated with single dose (3 ml) of Haemorrhagic septiceaemia (Pasteurella multocida P52 strain) oil adjuvant vaccine. The cumulative group mean serum -tocopherol concentration () was 88.12, 210.11, 235.21 and 294.02 in-groups I, II, III and IV, respectively and differed significantly (p<0.001) among the four groups. Lymphocyte stimulation indices (LSI) did not differ among the groups significantly. The pooled mean ELISA antibody titer against Pasteurella multocida (P52 strain) was 788.02, 926.85, 1,214.00 and 1,109.51 for group I, II, III and IV, respectively, which indicated higher antibody titer in groups supplemented with vitamin E as compared to the control group. It may be concluded that vitamin E supplementation increased the -tocopherol concentration in serum and dietary supplementation of vitamin E at higher level has a humoral immune enhancing effect against killed bacterial antigen.
 Keywords
Vitamin E;Serum -Tocopherol;Crossbred Calves;Immune Response;ELISA;LTT;
 Language
English
 Cited by
 References
1.
Afzal, M., R. P. Tengerdy, R. P. Ellis, C. V. Kimberling and C. J. Morris. 1984. Protection of rams against epididymitis by a B. ovis-vitamin E adjuvant vaccine. Vet. Immunol. Immunopathol. 7:293-304 crossref(new window)

2.
Alderson, N. E., G. E. Mitchell, C. O. Little, R. E. Warner and R. E. Tucker. 1971. Preintestinal disappearance of vitamin E in ruminants. J. Nutr. 101:655-660

3.
Almedia, J. D., P. S. Atanasiu, J. E. Gerdner, A. W. Schuurs, A. Vollar and R. H. Yolken. 1979. Manual for laboratory viral diagnosis. WHO offset publication No. 47, WHO, Geneva

4.
Bain, R. V. S., M. C. L. DeAlwis, G. R. Carter and B. K. Gupta. 1982. Haemorrhagic septicaemia. FAO, United Nations, No. 33, Rome

5.
Beisel, W. R. 1982. Single nutrient and immunity. Am. J. Clin. Nutr. 35:417

6.
Chatterjee, P. N., H. Kaur and N. Panda. 2003. Effect of vitamin E supplementation on plasma antioxidant vitamin and immunity status of crossbred cows. Asian-Aust. J. Anim. Sci. 16:1614- 1618

7.
Cipriano, J. E., J. L. Morrill and N. V. Anderson. 1982. Effect of dietary vitamin E on immune responses of calves. J. Dairy Sci. 65:2357-2365

8.
Coelho, M. B. 1991. Vitamin Stability in Premixes and Feeds. A practical approach. BASF Technical symposium, Bloomington, Minnesota, p. 56

9.
Corwin, L. M. and R. K. Gordon. 1982. Vitamin E and immune regulation. Ann. New York Acad. Sci. 393:437 crossref(new window)

10.
Cuesta, P. A., L. R. McDowell, W. E. Kunkle, M. J. Lawman, F. Bullock, A. Drew, N. S. Wilkinson and F. G. Martin. 1996.Mineral status in beef cattle, live weight changes and immune response as related to selenium and vitamin E supplementation. Livestock Research for Rural Development 8:1-7

11.
Drugs and Cosmetics Acts and Rules (VI Amendment). 1994. Government of India

12.
Franchini, A., M. Canti, G. Manfreda and S. Bertuzzi. 1991. Vitamin E as adjuvant in emulsified vaccines for chicks. Poult. Sci. 70:709-713

13.
Frigg, M. and H. E. Gallo-Torres. 1977. Phagocytosis of $\alpha$- tocopheryl nicotinate by the reticulo-endothelial system. A micro-auto radiographic study. Int. J. Vit. Nutr. Res. 47:145

14.
Heinzerling, R. H. 1974. The effect of vitamin E on immunity, Ph.D. Dissertation, Colorado State Univ., Fort Collins, USA

15.
Hill, G. A. 1987. Vitamin E and selenium supplementation of cattle. In: Proc. Georgia Nutrition Conference for the Feed Industry. Agriculture Experimental Station, Univ. of Georgia, Athens, pp. 57-65

16.
Hill, G. M., R. L. Stuart, P. R. Utley and J. O. Reagan. 1990. Vitamin E effects on finishing steer performance. J. Anim. Sci. 68 (Suppl. 1):557 (Abstr)

17.
Hill, G. M., S. E. Williams, S. N. Williams, S. W. Neel and P. R. Utley. 1993. Vitamin E effects on steer performance and color retention of steaks and ground beef. J. Anim. Sci. 71 (Suppl 1):22 (Abstract)

18.
Kahlon, R. S. and Raj Vir Singh. 2004. Effect of alpha tocopherol supplementation on plasma levels of antioxidant vitamins in anestrus buffalo (Bubalus bubalis) Asian-Aust. J. Anim. Sci. 17:1088-1092

19.
Larsen, H. J. and S. Tollersrud. 1981. Effect of dietary vitamin E and selenium on the phytohemagglutinin response of pig lymphocytes. Res. Vet. Sci. 31:301

20.
Lowry, O. H., N. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with the folin phenol reagents. J. Biol. Chem. 193:265-275

21.
McMurray, C. H. and W. J. Blanchflower. 1979. Application of a high performance liquid chromatographic fluorescence method for the rapid determination of $\alpha$-tocopherol in the plasma of cattle and pigs and its comparison with direct fluorescence and High Performance Liquid Chromatography-Ultraviolet detection methods. J. Chromatography 178:525-531 crossref(new window)

22.
Mondal, B. C. 1996. Performance of crossbred (Bos indicusx Bos taurus) calves on grain less diet during milk and post milk feeding period. Ph.D. thesis, Deemed Univ. IVRI, Izatnagar, India

23.
Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival, application to proliferation and cytotoxicity assays. J. Immunological Methods 65:55-63 crossref(new window)

24.
Muneer, R., S. Akhtar and H. Afzal. 1994. Evaluation of three oil adjuvant vaccines against P. multocida in buffalo calves. Revue Scientifique at Technique OIE, 13:837-843

25.
NRC. 1989. Nutrient requirements of domestic animals: Nutrient requirements of beef cattle, 6th ed. National Academy of Sciences - National Research Council, Washington, DC

26.
NRC. 2001. Nutrient requirements of dairy cattle. Seventh revised ed. National Research Council, Washington, DC

27.
Orth, C. K. 1992. The glucocorticoids suppress immunity. New Scientists 205:311-313

28.
Panganmala, R. V. and D. G. Cornwell. 1982. The effects of vitamin E on arachidonic acid metabolism. Ann N.Y. Acad. Sci. 393:376-90 crossref(new window)

29.
Pati, U. S. 1994. Characterisation of protective antigen of Pasteurella multocida serotype 6B. M.V.Sc. Thesis, Deemed Univ., IVRI, Izatnagar, India

30.
Paulson, G. D., G. A. Broderick, C. A. Baumann and A. L. Pope. 1968. Effect of feeding sheep with selenium fortified trace mineralized salt: effect of tocopherol. J. Anim. Sci. 27:195

31.
Pehrson, B., J. Hakkarainen, K. E. Tornquist and C. Fossum. 1991. Effect of vitamin E supplementation on weight gain, immune competence, and disease incidence in barley fed cattle. J. Dairy Sci. 74:1054-1059

32.
Rana, R. and N. C. Srivastava. 1999. Immune response in rabbits (Lepus cuniculus) immunized with Mycoplasma mycoides sub sp. Capri in different adjuvants. Ind. J. Vet. Res. 8:45-53

33.
Rawat, M. and T. N. Jaiswal. 2004. Immunomodulation of water in oil in water (w/o/w), emulsion vaccines of killed pasteurella multocida (P52) whole cell grown under iron-regulated condition. Ind. J. Anim. Sci. 74(7):701-705

34.
Reddy, P. G., J. L. Morrill, H. C. Minocha and J. S. Stevenson. 1987. Vitamin E is immunostimulatory in calves. J. Dairy Sci. 70:993

35.
Reddy, P. G., J. L. Morrill, H. C. Minocha, R. A. Frey, M. B. Morill and A. D. Dayton. 1985. Effect of serum from vitamin E supplemented calves on infectious bovine rhinotrachatis virus replication. J. Dairy Sci. 68(Suppl 1):146

36.
Reddy, P. G., J. L. Morrill, H. C. Minocha, M. B. Morrill, A. D. Dayton and R. A. Frey. 1986. Effect of supplemental vitamin E on the immune system of calves. J. Dairy Sci. 69:164

37.
Rivera, J. D., G. C. Duff, M. L. Galyean, D. A. Walker and G. A. Nunnery. 2002. Effect of supplemental vitamin E on performance, health and humoral immune response of beef cattle. J. Anim. Sci. 80:933-941

38.
Segagni, E. 1955. Vitamin E effect on vaccination. Minerva Pediat. 7:985-987

39.
Sheffy, B. E. and Alma J. Williams. 1982. Nutrition and the immune response. J. Am. Vet. Med. Assoc. 180:1073

40.
Siddons, R. C. and C. F. Mills. 1981. Glutathione peroxidase activity and erythrocyte stability in calves differing in selenium and vitamin E status. Br. J. Nutr. 46:345 crossref(new window)

41.
Snedecor, G. W. and W. G. Cochran. 1980. Statistical methods, 7th Ed. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi

42.
Steel, R. G. D. and J. H. Torrie. 1980. Principles and Procedures of Statistics .A Biometrical Approach, 2nd ed., McGraw Hill Book Company, New York, USA

43.
Spratt, M. G. and C. C. Kratzing. 1971. The effect of dietary lipid and $\alpha$-tocopherol on RES activity in choline deficiency. J. Reticuloendothel. Soc. 10:319

44.
Tizzard, I. 1995. Immunology, an introduction, 5th edn. W.B. Saunders Co., Philadelphia

45.
Tengerdy, R. P., E. Ameghino and H. Riemann. 1991. Serological response of rams to a Brucella ovis vitamin E adjuvant vaccine. Vaccine, 9:273-276 crossref(new window)

46.
Tengerdy, R. P., D. L. Meyer, L. H. Lauerman, D. C. Lueker and C. F. Nockels. 1983. Vitamin E enhanced humoral antibody response to Clostridia perfringens type D in sheep. Br. Vet. J. 139:147-152

47.
Walsh, M. D., D. G. Kennedy, E. A. Goodall and S. Kennedy. 1993. Antioxidant enzyme activity in the muscles of calves depleted of vitamin E or selenium or both. Br. J. Nutr. 70:621-630 crossref(new window)