JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effect of Tween 80 on Hydrolytic Activity and Substrate Accessibility of Carbohydrolase I (CBH I) from Trichoderma viride
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Tween 80 on Hydrolytic Activity and Substrate Accessibility of Carbohydrolase I (CBH I) from Trichoderma viride
Kim, Wanjae; Gamo, Yuko; Sani, Yahaya Mohammed; Wusiman, Yimiti; Ogawa, Satoru; Karita, Shuichi; Goto, Masakazu;
  PDF(new window)
 Abstract
The present study examined the effects of Tween 80 on the attachment and hydrolytic activity of a cellulase enzyme against ball-milled cellulose (BMC), using the whole component (native CBH I) and the catalysis module (core CBH I) of carbohydrolase I purified from Trichoderma viride (Meicelase, Meiji Seika, Tokyo, Japan). The effects were evaluated as protein concentrations in the supernatant after mixing enzyme and substrate with Tween 80 at room temperature. Tween 80 decreased the adsorption of native CBH I and core CBH I onto BMC (p<0.001) and increased the amount of reducing sugars released from BMC by native CBH I (p<0.001). However, Tween 80 did not enhance the hydrolytic activity of core CBH I. Observations using SEM revealed that Tween 80 caused cellulose filter paper to swell and enhanced surface cracks and filaments caused by native CBH I but not by core CBH I. These results suggested that Tween 80 decreases enzyme adsorption to its substrate but enhances enzymatic activity.
 Keywords
Enzymatic Activity;Cellobiohydrolase;Tween 80;
 Language
English
 Cited by
 References
1.
Aksenova, L. A., M. V. Dunaeva, E. A. Zak, Y. F. Osipov and N. L. Klyachko. 1994. The effect of Tween 80 on seed germination in winter wheat cultivars differing in drought resistance. Russian J. Plant Physiol. 41:557-559

2.
Alkasrawi, M., T. Eriksson, J. Börjesson, A. Wingren, M. Galbe, F. Tjerneld and G. Zacchi. 2003. The effect of Tween-20 on simultaneous saccharification and fermentation of softwood to ethanol. Enzyme Microb. Technol. 33:71-78 crossref(new window)

3.
Bayer, E. A., L. J. W. Shimon, Y. Shoham and R. Lamed. 1998. Cellulosomes-Structure and Ultrastructure. J. Stru. Biol. 124:221-234 crossref(new window)

4.
Bhikhabhai, R and G. Pettersson. 1994. The disulphide bridges in Cellobiohydrolase and and endoglucanase from Trichoderma ressei. Biochem. J. 222:729-736

5.
Bosworth, K., K. Firestone and S. Robertson. 2001. Scanning Electron Microscope Manual. Ver. 3. Rice University, Houston, TX, USA

6.
Castanon, M. and C. R. Wilke. 1981. Effects of the surfactant Tween 80 on enzymatic hydrolysis of newspaper. Biotechnol. Bioeng. 23:1365-1372 crossref(new window)

7.
Eriksson, T., J. Börjesson and F. Tjerneld. 2002. Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose. Enzyme Microb. Technol. 31:53-364 crossref(new window)

8.
Fendler, J. and E. Fendler. 1975. Catalysis in Micellar and Macromolecular Systems. Academic Press, New York

9.
Goto, M., H. Bae, S. S. Lee, M. S. Yahaya, S. Karita, K. Wnajae and K. J. Cheng. 2003a. Effects of surfactant Tween 80 on forage degradability and microbial growth on the in vitro rumen mixed and pure culture. Asian-Aust. J. Anim. Sci. 16(5):672-676

10.
Goto, M., Hee-Dong Bae, M. S. Yahaya, S. Karita, W. Kim, J. Baah, K. Sugawara and K. J. Cheng. 2003b. Effects of surfactant Tween 80 on enzymatic accessibility and degradation of orchardgrass (Dactylis glomerata L.) at different growth stages. Asian-Aust. J. Anim. Sci. 16(1):83-87

11.
Helle, S. S., S. J. Duff, B and D. G. Cooper. 1993. Effect of surfactants on cellulose hydrolysis. Biotechnol. Bioeng. 42:611-617 crossref(new window)

12.
Kamade, G. M., J. Baah, K. J. Cheng, T. A. Mcallister and J. A. Shelford. 2000. Effect of Tween 60 and Tween 80 on protease activity, thiol group reactivity, protein adsorption, and cellulose degradation by rumen microbial enzymes. J. Daily Sci. 83(3):53-542

13.
Karr, W. E. and M. Holtzapple. 1998. Benefit from Tween during enzymic hydrolysis of corn stover. Biotechnol Bioeng. 28:1727-1734 crossref(new window)

14.
Kim, M. H., S. B. Lee and D. Y. Ryu. 1982. Surface deactivation of cellulase and its preventation. Enzyme Microb. Technol. 4:99-103 crossref(new window)

15.
Kim, W., Y. Gamo, M. S. Yahaya, W. Yimiti and M. Goto. 2005. Pretreatment of Surfactant Tween 80 and Fibrolytic Enzyme to Influence Volatile Fatty Acid and Methane Production of a Total Mixed Ration by Mixed Rumen Microorganisms. J. Japan Grassl. Sci. 5(2):145-152

16.
Lee, S. S. and J. K. Ha. 2003. Influence of surfactant Tween 80 on the gas production, cellulose digestion and enzyme activities by mixed rumen microorganism. Asian-Aust. J. Anim. Sci. 16:83-87

17.
Lee, S. S., B. H. Ahn, H. S. Kim, C. H. Kim, K. J. Cheng and J. K. Ha. 2003. Effects of non-ionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performances of lactating cows. Asian-Aust. J. Anim. Sci. 16:104-115

18.
Lowry, O. H., N. J. Rosebrough, A. Lewis Farr and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275

19.
Lemos, M. A., J. A. Teixeira, M. R. M. Domingues, M. Mota and F. M. Gama. 2003. The enhancement of cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from Trichoderma reesei. Enzyme Microb. Technol. 32:35-40 crossref(new window)

20.
Madamwar, D., A. Patel and V. Patel. 1991. Effect of various surfactants on anaerobic digestion of water hyacinth-cattle drug. Biores. Technol. 37:157-160 crossref(new window)

21.
Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31:426-428 crossref(new window)

22.
Ooshima, H., M. Sakata and Y. Harano. 1986. Enhancement of enzymatic hydrolysis of cellulose by surfactant. Biotechnol. Bioeng. 28:1727-1734 crossref(new window)

23.
Panda, T., H. Gruder and C. P. Kubicek. 1987. Stimulation of protein secretion in Trichoderma reesei by Tween surfactants is not correlated with changes in enzyme localization or membrane fatty acid composition. FEMS Microb. Lett. 41:85- 90 crossref(new window)

24.
Park, J. W., Y. Takahata, T. Kajiuchi and T. Akehata. 1991. Effect of nonionic surfactant on enzymatic hydrolysis of used news paper. Biotechnol. Bioeng. 39:117-120 crossref(new window)

25.
Saloheimo, M., T. Nakari-Setälä, M. Tenkanen and M. Penttilä. 1997. cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast. Eur. J. Biochem. 249:584-591 crossref(new window)

26.
SAS Institute Inc. 1989. SAS/STAT User's Guide: Version 6. 4th end. SAS institute Inc., Cary, North, California

27.
Srisodsuk, M. 1994. Mode of action of Trichoderma reesei cellobiohydrolase I on crystalline cellulose. Academic dissertation. VTT Publications 188. Technical Research Centre of Finland, Espoo

28.
Takano, M., R. Moriyama and K. Ohmiya. 1992. Structure of a $\beta$- glucosidase gene Ruminococcus albus and protein of translated product. J. Fermen. Bioeng. 73(2):79-88

29.
Tomme, P., H. Van Tilgeurgh, G. Pettersson, J. Van Damme, J. Vandekerckhove, J. Knowles, T. Terri and M. Claeyssens. 1988. Studies of the cellulolytic system of Trichoderma reesei QM 9414; Analysis of domain function in two cellobiohydrolysis by limited proteolysis. Eur. J. Biochem. 170:575-578 crossref(new window)

30.
Van Tilgeurgh, H., P. Tomme, M. Claeyssens, R. Bhikhabhai and G. Pettersson. 1986. Limited Proteolysis of the cellobiohydrolase I from Trichoderma reesei. FEBS letters. 204(2):223-227 crossref(new window)

31.
Wang, Y., T. A. McAllister, J. Baah, R. Wilde, K. A. Beauchemin, L. M. Rode, J. A. Shelford, G. M. Kamande and K. J. Cheng. 2003. Effect of Tween 80 on in vitro fermentation of silage and interactive effects of Tween 80, Monensin and exogenous fibrolytic enzyme on growth performance by feedlot cattle. Asian-Aust. J. Anim. Sci. 16:968-978