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Effect of Forage to Concentrate Ratio and Monensin Supplementation on cis-9, trans-11 Conjugated Linoleic Acid and trans-11 Octadecenoic Acid Concentrations of Ruminal Contents and Plasma in Sheep
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 Title & Authors
Effect of Forage to Concentrate Ratio and Monensin Supplementation on cis-9, trans-11 Conjugated Linoleic Acid and trans-11 Octadecenoic Acid Concentrations of Ruminal Contents and Plasma in Sheep
Zhang, Yuzhi; Kong, Xianghao; Zhu, Xiaoping; Wang, Runlian; Yan, Yichai; Jia, Zhihai;
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 Abstract
Twenty-four cannulated Small-tailed HanPoll Dorset wethers (BW ) were used to determine the effects of forage to concentrate ratio (40:60 vs. 70:30), monensin supplementation (0, 15 or 30 ppm, DM basis) and interactions of these two factors on cis-9, trans-11 conjugated linoleic acid (cis-9, trans-11 CLA) and trans-11 octadecenoic acid (trans11-) concentrations in ruminal contents and plasma in sheep. The experiment was designed as a factorial. The diet contained Chinese wild rye grass hay (Aneurolepidium Chinese), cracked corn, soybean meal, NaCl, limestone and trace mineral premix. Dietary crude fat and linoleic acid () were adjusted with soybean oil to about 7.0% and 24.0 mg/g (DM basis), respectively. High forage diets increased (p<0.001) the concentrations of trans11- and cis-9, trans-11 CLA in ruminal contents and plasma. Monensin supplementation increased (p<0.001) the concentration of trans11- in ruminal contents, but had no effect on that of cis-9, trans-11 CLA. Concentrations of trans11- (p<0.019) and cis-9, trans-11 CLA (p<0.022) in plasma increased with dietary monensin levels. Interactions of forage: concentrate ratio and monensin level tended to affect the concentrations of trans11- (p<0.091) and (p<0.083) in ruminal contents. Increasing forage levels increased the concentrations of trans11- and cis-9, trans-11 CLA in the rumen. Supplementing with monensin increased the ruminal production of trans11- and concentrations of trans11- and cis-9, trans-11 CLA in plasma.
 Keywords
cis-9;trans-11 CLA;trans11-;Forage to Concentrate Ratio;Monensin;Rumen;Plasma;
 Language
English
 Cited by
 References
1.
An, B. K., C. W. Kang, Y. Izumi, Y. Kobayashi and K. Tanaka. 2003. Effects of dietary fat sources on occurrences of conjugated linoleic acid and trans fatty acids in ruminal contents. Asian-Aust. J. Anim. Sci. 16(2):222-256

2.
Beaulieu, A. D., J. K. Drackley and N. R. Merchen. 2002. Concentrations of conjugated linoleic acid(cis-9, trans-11- octadecadienoic acid)are not increased in tissue lipids of cattle fed a high-concentrate diet supplemented with soybean oil. J. Anim. Sci. 80:847-861

3.
Choi, S. H. and M. K. Song. 2005. Effect of C18-polyunsaturated fatty acids on their direct incorporation into the rumen bacterial lipids and CLA production in vitro. Asian-Aust. J. Anim. Sci. 18(4):512-515

4.
Fellner, V., F. D. Sauer and J. K. G. Kramer. 1997. Effect of nigericin, monensin, and tetronasin on biohydrogenation in continuous flow-through ruminal fermenters. J. Dairy Sci. 80:921-928

5.
Folch, J., M. Lees and G. H. Sloane-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509

6.
Griinari, J. M. and D. E. Bauman. 1999. Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In: Advances in conjugated linoleic acid research, Volume 1, (Ed. M. P. Yurawecz, M. M. Mossoba, J. K. G. Kramer, G. J. Nelson and G. J. Nelson). AOCS press, Illinois, Chapter 13, pp. 180-200

7.
Griinari, J. M., B. A. Corl, S. H. Lacy, P. Y. Chouinard, K. V. V. Nurmela and D. E. Bauman. 2000. Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by ${\Delta}^9$-desaturase. J. Nut. 130:2285-2291

8.
Harfoot, C. G. 1981. Lipid metabolism in the rumen. In: Lipid metabolism in ruminant animals, (Ed. W. W. Christie). Pergamon press, New York, pp. 21-55

9.
Jenkins, T. C., V. Feller and R. K. McGuffey. 2003. Monensin by fat interactions on trans fatty acids in cultures of mixed ruminal microorganisms grown in continuous fermentors fed corn or barley. J. Dairy Sci. 86:324-330

10.
Kalscheur, K. F., B. B. Teter, L. S. Piperova and R. A. Erdman. 1997. Effect of dietary forage concentration and buffer addition on duodenal flow of trans-$C_{18:1}$ fatty acids and milk fat production in dairy cows. J. Dairy Sci. 80:2104-2114

11.
Kepler, C. R., W. P. Tucker and S. B. Tove. 1970. Biohydrogenation of unsaturated fatty acids. IV. Substrate specificity and inhibition of linoleate delta 12-cis, delta 11- trans isomerase from Butyrivibrio fibrisolvens. J. Biol. Chem. 245:3612-3620

12.
Khanal, R. C. 2004. Potential health benefits of conjugated linoleic acid (CLA): a review. Asian-Aust. J. Anim. Sci. 17(9):1315-1328

13.
Kim, T. W., N. J. Choi, J. Hwangbo, Jih Tay Hsu, S. S. Lee, M. K. Song, I. J. Seo and Y. J. Kim. 2005. Production of trans-10, cis-12 conjugated linoleic acid by Megasphaera elsdenii YJ-4: physiological roles in the rumen. Asian-Aust. J. Anim. Sci. 18(10):1425-1429

14.
Kim, Y. J. 2003. Partial inhibition of biohydrogenation of linoleic acid can increase the conjugated linoleic acid production of Butyrivibrio fibrisolvens A38. J. Agric. Food. Chem. 51:4258- 4262 crossref(new window)

15.
Kucuk, O., B. W. Hess, P. A. Ludden and D. C. Rule. 2001. Effect of forage:concentrate ratio on ruminal digestion and duodenal flow of fatty acids in ewes. J. Anim. Sci. 79:2233-2240

16.
Martin, S. A. and T. C. Jenkins. 2002. Factors affecting conjugated linoleic acid and trans-C18:1 fatty acid production by mixed ruminal bacteria. J. Anim. Sci. 80:3347-3352

17.
NRC. 1985. Nutrient requirements of sheep. (6th ed.). National Academy of Science. Washington, DC

18.
Qiu, X., M. L. Eastridge, K. E. Griswold and J. L. Firkins. 2004. Effects of substrate, passage rate and pH in continuous culture on flows of conjugated linoleic acid and trans C18:1. J. Dairy Sci. 87:3473-3479

19.
Russell, J. B. and R. L. Baldwin. 1979. Comparison of substrate affinities among several rumen bacteria: a possible determinant of rumen bacterial competition. Appl. Environ. Microbiol. 37:531-536

20.
Sackmann, J. R., S. K. Duckett, M. H. Gillis, C. E. Realini, A. H. Parks and R. B. Eggelston. 2003. Effects of forage and sunflower oil levels on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets. J. Anim. Sci. 81:3174-3181

21.
Van Nevel, C. and D. I. Demeyer. 1995. Lipolysis and biohydrogenation of soybean oil in the rumen in vitro: Inhibition by antimicrobials. J. Dairy Sci. 78:2797-2806

22.
Wang, J. H. and M. K. Song. 2001. Effect of sources and levels of carbohydrates on fermentation characteristics and biohydrogenation of linoleic acid by rumen bacteria in vitro. Asian-Aust. J. Anim. Sci. 14(1):48-53

23.
Wang, J. H. and M. K. Song. 2003. pH affects the in vitro formation of cis-9, trans-11 CLA and trans-11 octadecenoic acid by ruminal bacteria when incubated with oilseeds. Asian-Aust. J. Anim. Sci. 16(12):1743-1748

24.
Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002a. Effect of concentrate level on the formation of conjugated linoelic acid and trans-octadecenoic acid by ruminal bacteria when incubated with oilseeds in vitro. Asian-Aust. J. Anim. Sci. 15(5):687-694

25.
Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002b. Addition of seed-associated or free linseed oil on the formation of cis-9, trans-11 conjugated linoleic acid and octadecenoic acid by ruminal bacteria in vitro. Asian-Aust. J. Anim. Sci. 15(8):1115-1120

26.
Wang, J. H., S. H. Choi and M. K. Song. 2003. Effects of concentrate to roughage ratio on the formation of cis-9, trans- 11 CLA and trans-11-octadecenoic acid in rumen fluid and plasma of sheep when fed high oleic or high linoleic acid oils. Asian-Aust. J. Anim. Sci. 16(12):1604-1609

27.
Wang, J. H., S. H. Choi, C. G. Yan and M. K. Song. 2005. Effect of monensin and fish oil supplementation on biohydrogenation and CLA production by rumen bacteria in vitro when incubated with safflower oil. Asian-Aust. J. Anim. Sci. 18(2):221-225

28.
Wu, Z., O. A. Ohajuruka and D. L. Palmquist. 1991. Ruminal synthesis, biohydrogenation, and digestibility of fatty acids by dairy cows. J. Dairy Sci. 74:3025-3034