Advanced SearchSearch Tips
Effects of Arsenic (AsIII) on Lipid Peroxidation, Glutathione Content and Antioxidant Enzymes in Growing Pigs
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Arsenic (AsIII) on Lipid Peroxidation, Glutathione Content and Antioxidant Enzymes in Growing Pigs
Wang, L.; Xu, Z.R.; Jia, X.Y.; Jiang, J.F.; Han, X.Y.;
  PDF(new window)
This experiment was conducted to investigate the effect of arsenic () on lipid peroxidation, glutathione content and antioxidant enzymes in growing pigs. Ninety-six Duroc-Landrace-Yorkshire crossbred growing pigs (48 barrows and 48 gilts, respectively) were randomly assigned to four groups and each group was randomly assigned to three pens (four barrows and four gilts). The four groups received the same corn-soybean basal diet which was supplemented with 0, 10, 20, 30 mg/kg As respectively. Arsenic was added to the diet in the form of . The experiment lasted for seventy-eight days after a seven-day adaptation period. Malondialdehyde (MDA) levels, glutathione (GSH) contents and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) activities were analyzed in serum, livers and kidneys of pigs. The results showed that pigs treated with 30 mg As/kg diet had a decreased average daily gain (ADG) (p<0.05) and an increased feed/gain ratio (F/G) (p<0.05) compared to the controls. The levels of MDA significantly increased (p<0.05), and the contents of GSH and the activities of SOD, CAT, GPx, GR and GST significantly decreased (p<0.05) in the pigs fed 30 mg As/kg diet. The results indicated that the mechanism of arsenic-induced oxidative stress in growing pigs involved lipid peroxidation, depletion of glutathione and decreased activities of some enzymes, such as SOD, CAT, GPx, GR and GST, which are associated with free radical metabolism.
Growing Pigs;Arsenic;Growth Performance;Lipid Peroxidation;Glutathione;Antioxidant Enzymes;
 Cited by
Effects of different selenium sources and levels on serum biochemical parameters and tissue selenium retention in rats, Frontiers of Agriculture in China, 2009, 3, 2, 221  crossref(new windwow)
Arsenic interactions with lipid particles containing iron, Environmental Geochemistry and Health, 2009, 31, S1, 201  crossref(new windwow)
Oxidative stress induced by the chemotherapeutic agent arsenic trioxide, 3 Biotech, 2014, 4, 4, 425  crossref(new windwow)
The effect of arsenic on some antioxidant enzyme activities and lipid peroxidation in various tissues of mirror carp (Cyprinus carpio carpio), Environmental Science and Pollution Research, 2015, 22, 5, 3212  crossref(new windwow)
Histopathological and biochemical effects of cyanobacterial cells containing microcystin-LR on Tilapia fish, Water and Environment Journal, 2016, 30, 1-2, 135  crossref(new windwow)
Arjunolic Acid Improves the Serum Level of Vitamin B12 and Folate in the Process of the Attenuation of Arsenic Induced Uterine Oxidative Stress, Biological Trace Element Research, 2017, 1559-0720  crossref(new windwow)
Betulinic acid, natural pentacyclic triterpenoid prevents arsenic-induced nephrotoxicity in male Wistar rats, Comparative Clinical Pathology, 2017, 1618-565X  crossref(new windwow)
Aebi, H. 1984. Catalase in vitro. Methods Enzymol. 105:121-126 crossref(new window)

Asada, K., M. Takahashi and M. Nagate. 1974. Assay and inhibitors of spinach suoeroxide dismutase. Agric. Biol. Chem. 38:471-473

Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248-254 crossref(new window)

Carlberg, I. and B. Mannervik. 1985. Glutathione reductase. Methods Enzymol. 113:484-485 crossref(new window)

Cunningham, M. L., M. J. Zvelebi and A. H. Fairlamb. 1994. Mechanism of inhibition of trypnothione reductase and glutathione reductase by trivalent arsenicals. Eur. J. Biochem. 221:285-295 crossref(new window)

Czarnecki, G. L. and D. H. Baker. 1985. J. Anim. Sci. 60(2):440- 450

Donoghue, D. J., H. Hairstone, C. V. Cope, M. J. Barthlomew, and D. D. Wagner. 1994. Incurred arsenc residules in chicken eggs. J. Food Prot. 57(3):218-223

Ellman, G. L., 1959. Tissue sulfhydryl groups. Arch. Biochem. 82:70-77 crossref(new window)

Falkner, K. C., G. P. McCallum, M. G. Cherian and J. R. Bend. 1993. Effects of acute sodium arsenite administration on the pulmonary chemical metabolizing enzymes, cytochrome P-450 monooxygenase, NAD(P)H: quinone acceptor oxidoreductase and glutathione-S-transferase in guinea pig: comparison with effects in liver and kidney. Chem. Biol. Interact. 86:51-68 crossref(new window)

Flohe, L. and W. A. Gunzler. 1984. Assays of glutathione peroxidase. Methods Enzymol. 105:114-121 crossref(new window)

Flora, S. J. S., Dubey Rupa, G. M. Kannan, R. S. Chauhan, B. P. Pant and D. K. Jaiswal. 2002. Meso 2,3-dimercaptosuccinic acid (DMSA) and monoisoamyl DMSA effect on gallium arsenide induced pathological liver injury in rats. Toxicol. Letters 132:9-17 crossref(new window)

Habig, W. H., M. J. Pabst and W. B. Jakoby. 1974. Glutathione Stransferases: the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249:7130-7139

Hei, T. K., C. R. Geard and E. J. Hall. 1984. Effects of cellular non-protein sulfhydryl depletion in radiation induced oncogenic transformation and genotoxicity in mouse 10T1/2 cells. Int. J. Radiat. Oncol. Biol. Phys. 10:1255-1259

Holcman, A., S. Malovrh and V. Knex. 2001.The effect of diet containing arsenic(III) oxide on the traits of eggs. Zbornik Biotechniske Fakalter Univerze Ljubljani. Kmetijstvo, Zootehnika 78(2):211-218

Holcman, A. and V. Stibilj. 1997.Asenic residuls in eggs from laying hens fed with a diet containing arsenic (III) oxide. Arch. Environ. Contam. Toxicol. 32(4):407-410 crossref(new window)

Hughes, M. F. 2002. Arsenic toxicity and potential mechanisms of action. Toxicol. Letters 33:1-16 crossref(new window)

IARC, 1987. Arsenic and arsenic compounds (Group 1). In: IARC monographs on the evaluation of the carcinogenic risks to humans. Supplement 7, date accessed: 6 February 2003

Imlay, J. A. and S. Linn. 1988. DNA damage and oxygen radical toxicity. Sci. 240:1302-1309 crossref(new window)

Lee, T. C. and I. C. Ho. 1995. Modulation of cellular antioxidant defense activities by sodium arsenite in human fibroblasts. Arch. Toxicol. 69:498-508 crossref(new window)

Keyse, S. M. and R. M. Tyrell. 1989. Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide and sodium arsenite. Proc. Natl. Acad. Sci. USA 86:99-103

Kirkman, M. N. and G. F. Gaetani. 1984. Catalase: a tetrameric enzyme with four tightly bound molecules of NADPH. Proc. Natl. Acad. Sci. USA. 81:4343-4347

Kono, Y. and I. Fridovich. 1982. Superoxide radicals inhibit catalase. J. Biol. Chem. 257:5751-5754

Liu, L., J. R. Trimarchi, P. Navarro, M. A. Blasco and D. L. Keefe. 2003. Oxidative stress contributes to arsenic induced telomere attrition, chromosomal instability and apoptosis. J. Biol. Chem. 278:31998-32004 crossref(new window)

Liu, S. X., M. Athar, I. Lippai, C. Waldren and T. K. Hei. 2001. Induction of oxygen radicals by arsenic: implications for mechanism of genotoxicity. Proc. Natl. Acad. Sci. USA. 98:1643-1648

Maiti, S. and A. K. Chatterjee. 2000. Differential response of cellular antioxidant mechanism of liver and kidney to arsenic exposure and its relation to dietary protein deficiency. Environ. Toxicol. Pharm. 8:227-235 crossref(new window)

Maiti, S. and A. K. Chatterjee. 2001. Effects on levels of glutathione and some related enzymes in tissues after an acute arsenic exposure in rats and their relationship to dietary protein deficiency. Arch. Toxicol. 75(9):531-537 crossref(new window)

Mandal, B. K. and K. T. Suzuki. 2002. Arsenic round the world: a review. Talanta 58:201-235 crossref(new window)

Morrison, L. L. and E. R. Chaves. 1983. Selenlum-arsenic interaction in the weanling pigs. Can. J. Anim. Sci. 63(1):239- 246

Ng, J. C., L. Qi, J. Wang, X. Xiao, M. Shahin, M. R. Moore and A. S. Prakash. 2001. Mutations in C57BI/6J and metallothionein knock-out mice induced by chronic exposure to sodium arsenate in drinking water. In: (W. R. Chappell, C. O. Abernathy and R. L. Calderon). Arsenic Exposure and Health Effects. Elsevier, pp. 231-242

Ng, J. C., A. A. Seawright, L. Qi, C. M. Garnett, B. Chiswell and M. R. Moore. 1999. Tumors in mice induced by exposure to sodium arsenate in drinking water. In: (Ed. W. R. Chappell, C. O. Abernathy and R. L. Calderon), Arsenic Exposure and Health Effects. Elsevier, pp. 217-223

Ramos, O., L. Carrizales, L. Yanez, L. Mejia, L. Batres, D. Ortiz and F. Diaz-Barriga. 1995. Arsenic increased lipid peroxidation in rat tissues by a mechanism independent of glutathione levels. Environ. Health Perspect. 103(Suppl 1):85- 98

Searle, A. J. and R. Wilson. 1980. Glutathione peroxide effect of hydroxyl and bromine free radicals on enzyme activity. Int. J. Radiat. Biol. 37:213-217 crossref(new window)

Singh, T. S. and K. K. Pant. 2004. Equilibrium, kinetics and thermodynamic studies for adsorption of As(III) on activated alumina. Separation and Purification Technol. 36:139-147 crossref(new window)

Styblo, M., S. V. Serves, W. R. Cullen and D. J. Thomas. 1997. Comparative inhibition of yeast glutathione reductase by arsenicals and arsenothiols. Chem. Res. Toxicol. 10:27-33 crossref(new window)

Vreman, K., N. G. van der Veen, E. J. van der Mollen and W.G. de Ruig. 1986. Transfer of cadmium, lead, mercury and arsenic from feed into milk and various tissues of dairy cows: chemical and pathological data. Netherlands Journal of Agric. Sci. 34(2):129-144

Wang, T. S., Y. F. Shu, Y. C. Liu, K. Y. Jan and H. Huang. 1997. Glutathione peroxidase and catalase modulate the genotoxicity of arsenite. Toxicol. 121:229-37 crossref(new window)

Wang, T. S. and H. Huang. 1994. Active oxygen species are involved in the induction of micronuclei in XRS-5 cells. Mutagenesis 9:253-257 crossref(new window)

Wills, E. D. 1966. Mechanisms of lipid peroxide formation in animal tissues. Biochem. J. 99:667-676

Xu, A., L. J. Wu, R. Santella and T. K. Hei. 1999. Role of reactive oxygen species in the mutagenicity and DNA damage induced by crocidolite fibers in mammalian cells. Cancer Res. 59:5615- 5624

Yu, Shiguang and A. C. Beynen. 2000. High arsenic raises kidnek copper and lows plasma copper concentrstions in rats. Biol. Trace Element Res. 81:63-70 crossref(new window)

Zaman, K., R. S. MacGill, J. E. Johnson, S. Ahmad and R. S. Pardini. 1995. An insect model for assessing oxidative stress related to arsenic toxicity. Arch. Insect Biochem. Physiol. 29:199-210 crossref(new window)