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Association of Polymorphisms in Epidermal Growth Factor, Prostaglandin-endoperoxide Synthase 2 and Prolactin Receptor Genes with Semen Quality in Duroc Boars
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 Title & Authors
Association of Polymorphisms in Epidermal Growth Factor, Prostaglandin-endoperoxide Synthase 2 and Prolactin Receptor Genes with Semen Quality in Duroc Boars
Huang, S.Y.; Song, H.L.; Lin, E.-C.; Lee, W.C.; Chiang, J.C.; Tsou, H.L.;
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 Abstract
The quality characteristics of semen are important indicators of the fertility of a boar. Development of genetic markers for the semen quality in boars will be beneficial to the improvement of porcine fertility. We investigated the relationship between the polymorphisms of epidermal growth factor (EGF), prostaglandin-endoperoxide synthase 2 (PTGS2) and prolactin receptor (PRLR) genes, and semen quality traits in boars. The genomic DNA of 233 boars (157 Duroc and 86 Landrace) from a central testing station was subjected to genotyping for surveying gene frequency. The EGF, PTGS2 and PRLR genotypes were determined using the restriction fragment length polymorphism method. Thirty-seven normal, mature Duroc boars from an AI center were also genotyped and their semen quality traits were collected. The effect of genotype on semen quality traits was analyzed by the least-squares means method using data corrected for season. The frequencies of the AA genotype of EGF, PTGS2 and PRLR in Duroc boars were 0.14, 0.01 and 0.66, respectively. In Landrace, the frequencies of the AA genotype were 0.03, 0.09 and 0.62, respectively. Boars with the BB genotype in EGF, with the AB genotype in PTGS2 and with the AA genotype in PRLR had significantly better semen quality with a higher percentage of normal sperm and a lower percentage of immature sperm than those with other genotypes. These findings imply that polymorphisms of EGF, PTGS2 and PRLR genes might be used as markers for improving the semen quality of boars.
 Keywords
Boar;Epidermal Growth Factor Gene;Prolactin Receptor Gene;Prostaglandin-endoperoxide Synthase 2 Gene;Restriction Fragment Length Polymorphism;Semen Quality;
 Language
English
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 References
1.
Boonstra, J., P. Rijken, B. Humbel, F. Cremers, A. Verkleij and P. van Bergen en Henegouwen. 1995. The epidermal growth factor. Cell Biol. Int. 19:413-430 crossref(new window)

2.
Chen, M. Y., C.-F. Tu, S. Y. Huang, J. H. Lin, B. S. Tzang, H. L. Tsou, T. H. Hseu and W. C. Lee. 2005. Augmenting thermotolerance in primary skin fibroblasts from a transgenic pig with overexpressed porcine HSP70.2. Asian-Aust. J. Anim. Sci. 18:107-112

3.
Colenbrander, B., H. Feitsma and H. J. Grooten.1993. Optimizing semen production for artificial insemination in swine. J. Reprod. Fertil. (Supp.) 48:207-215

4.
Den Daas, N. 1992. Laboratory assessment of semen characteristics. Anim. Reprod. Sci. 28:87-94 crossref(new window)

5.
Diamandis, E. P., W. P. Arnett, G. Foussias, H. Pappas, S. Ghandi, D. N. Melegos, B. Mullen, H. Yu, J. Srigley and K. Jarvi. 1999. Seminal plasma biochemical markers and their association with semen analysis findings. Urol. 53:596-603 crossref(new window)

6.
Drogemuller, C., H. Hamann and O. Distl. 2001. Candidate gene markers for litter size in different German pig lines. J. Anim. Sci. 79:2565-2570

7.
Evans, G. J., E. Giuffra, A. Sanchez, S. Kerje, G. Davalos, O. Vidal, S. Illan, J. L. Noguera, L. Varona, I. Velander, O. I. Southwood, D. J. de Koning, C. S. Haley, G. S. Plastow and L. Andersson. 2003. Identification of quantitative trait loci for production traits in commercial pig populations. Genet. 164:621-627

8.
Georges, M. 2001. Recent progress in livestock genomics and potential impact on breeding programs. 55: 15-21 crossref(new window)

9.
Gladney, C. D., V. G. Martinez, R. K. Brumbaugh, B. J. DeGroot, R. C. Linville, A. M. Oomen, R. M. Huebinger, L. A. Messer, M. F. Allan and D. Pomp. 1999. Rapid Communication: Mapping of the prostaglandin-endoperoxide synthase 2 (PTGS2) gene to porcine chromosome 9 and bovine chromosome 16 by linkage analysis using novel PCR-RFLP. J. Anim. Sci. 77:787-788

10.
Hadley, M. E. 1996. Endocrinology. 4th Edn. Prentice Hall, New Jersey

11.
Henricks, D. M., A. J. Kouba, B. R. Lackey, W. R. Boone and S. L. Gray. 1998. Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biol. Reprod. 59:330-337 crossref(new window)

12.
Herrick, J. L. and H. L. Self. 1962. Evaluation of fertility in the bull and boar. Iowa State University Press, Ames, Iowa

13.
Huang, S. Y., M. Y. Chen, E. C. Lin, H. L. Tsou, Y. H. Kuo, C. C. Ju and W. C. Lee. 2002. Effects of single nucleotide polymorphisms in the 5'-flanking region of heat shock protein 70.2 gene on semen quality in boars. Anim. Reprod. Sci. 70:99-109 crossref(new window)

14.
Huang, S. Y., Y. H. Kuo, Y. P. Lee, H. L. Tsou, E. C. Lin, C. C. Ju and W. C. Lee. 2000. Association of heat shock protein 70 with semen quality in boars. Anim. Reprod. Sci. 63:231-240 crossref(new window)

15.
Li, F. G., Y. Z. Xiong, C. Y. Deng, S. W. Jiang and R. Zheng. 2002. Frequencies, inheritance of porcine FSH- retroposon and its association with reproductive traits. Asian-Aust. J. Anim. Sci. 15:179-183

16.
Li, F. G., M. G. Lei, R. Zheng, B. Zuo, S. W. Jiang, C. Y. Deng and Y. Z. Xiong. 2004. The effects of estrogen receptor locus on reproductive tracts components and performance traits in large white meishan f2 offspring. Asian-Aust. J. Anim. Sci. 17:1223-1226

17.
Lim, H., B. C. Paria, S. K. Das, J. E. Dinchuk, R. Langenbach, J. M. Trzaskos and S. K. Dey. 1997. Multiple female reproductive failures in cyclooxygenase 2-deficient mice. Cell 91:197-208 crossref(new window)

18.
Linville, R. C., D. Pomp, R. K. Johnson and M. F. Rothschild. 2001. Candidate gene analysis for loci affecting litter size and ovulation rate in swine. J. Anim. Sci. 79:60-67

19.
Malmgren, L. and K. Larsson. 1984. Semen quality and fertility after heat stress in boars. Acta. Vet. Scand. 25:425-435

20.
Mendez, E. A., L. A. Messer, N. J. Larsen, A. Robic and M. F. Rothschild. 1999. Rapid communication: Epidermal growth factor maps to pig chromosome 8. J. Anim. Sci. 77:494-495

21.
Niwa, T. 1961. Researches and practices in artificial insemination of pig. Proceedings of the 4th International Congress on Animal Reproduction. 1:83-88

22.
Popwell, J. M. and W. L. Flowers. 2004. Variability in relationships between semen quality and estimates of in vivo and in vitro fertility in boars. Anim. Reprod. Sci. 81:97-113 crossref(new window)

23.
Rothschild, M. F., C. Jacobson, D. A. Vaske, C. Tuggle, L. Wang, T. Short, G. Eckardt, S. Sasaki, A. Vincent, D. G. McLaren, O. I. Southwood, H. van der Steen, A. Mileham and G. Plastow. 1996. The estrogen receptor locus is associated with a major gene influencing litter size in pigs. Proc. Natl. Acad. Sci. USA 93:201-205

24.
Rothschild, M. F. 2004. Porcine genomics delivers new tools and results: this little piggy did more than just go to market. Genet. Res. 83:1-6 crossref(new window)

25.
SAS Institute. 1989. SAS User's Guide: Statistics, Version 6.03. SAS Institute Inc., Cary, NC

26.
van Rens, B. T. T. M. and T. van der Lende. 2002. Liter size and piglet traits of gilts with different prolactin receptor genotypes. Theriogenol. 57:883-893 crossref(new window)

27.
Vignal, A., D. Milan, M. SanCristobal and A. Eggen. 2002. A review on SNP and other types of molecular markers and their use in animal genetics. Genet. Sel. Evol. 34:275-305

28.
Vincent, L., G. Evans, T. H. Short, O. I. Southwood, G. S. Plastow, C. K. Tuggle and M. F. Rothschild. 1998. The prolactin receptor gene is associated with increased litter size in pigs. In: Proceedings of the 6th World Congress on Genetics Applied in Livestock Production. 27:15-18

29.
Yun, J. S., W. J. Kang, D. S. Seo, S. S. Park, K. C. Hong, C. Y. Le, and Y. Ko. 2001. Association of endocrine factors (insulin-like growth factor-II and binding protein-3) with litter size in pigs. Asian-Aust. J. Anim. Sci. 14:307-315

30.
Zhang, S. J., Y. Z. Xiong, C. Y. Den, S. M. Xiao, J. X. Xu, Y. Xia, X. H. Liu, C. F. Wang and S. L. Sun. 2004. FSHR gene mutation and its effect on litter size in pigs. Asian-Aust. J. Anim. Sci. 17:179-182