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Effects of Urea Level and Sodium DL-malate in Concentrate Containing High Cassava Chip on Ruminal Fermentation Efficiency, Microbial Protein Synthesis in Lactating Dairy Cows Raised under Tropical Condition
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 Title & Authors
Effects of Urea Level and Sodium DL-malate in Concentrate Containing High Cassava Chip on Ruminal Fermentation Efficiency, Microbial Protein Synthesis in Lactating Dairy Cows Raised under Tropical Condition
Khampa, S.; Wanapat, Metha; Wachirapakorn, C.; Nontaso, N.; Wattiaux, M.;
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Four, lactating dairy cows were randomly assigned according to a Factorial arrangement in a Latin square design to study supplementation of urea level (U) at 2 and 4% and sodium dl-malate (M) at 10 and 20 g/hd/d in concentrate. The treatments were as follows U2M10, U2M20, U4M10 and U4M20, respectively. The cows were offered the treatment concentrate at a ratio to milk yield at 1:2.5 and urea-treated rice straw was fed ad libitum. The results have revealed that rumen fermentation and blood metabolites were similar for all treatments. The populations of protozoa and fungal zoospores were significantly different as affected by urea level and sodium dl-malate. In addition, the viable bacteria were similar for amylolytic and proteolytic bacteria. Cellulolytic bacteria were significantly affected by level of sodium dl-malate especially Selenomonas ruminantium and Megasphaera elsdenii while Butyrivibrio fibrisolvens was significantly affected by level of urea supplementation. In conclusion, the combined use of concentrate containing high level of cassava chip at 75% DM with urea at 4% in concentrate and sodium dl-malate at 20 g/hd/d with UTS as a roughage could improv rumen ecology and microbial protein synthesis efficiency in lactating dairy cows.
Urea;Sodium DL-malate;Rumen Fermentation;Cassava Chip;Urea-treated Rice Straw;Tropical Dairying;
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AOAC. 1985. Official Methods of Analysis. Association of Official Analysis Chemists, DC, USA

Asanuma, N., M. Iwamoto and T. Hino. 1999. Effect of the addition of fumalate on methane production by ruminal microorganisms in vitro. J. Dairy Sci. 82:780-787

Callaway, T. R. and S. A. Martin. 1996. Effects of organic acid and monensin treatment on in vitro mixed ruminal microorganism fermentation of cracked corn. J. Anim. Sci. 74:1982-1989

Chanjula, P., M. Wanapat, C. Wachirapakorn, S. Uriyapongson and P. Rowlinson. 2003. Ruminal degradability of tropical feeds and their potential use in ruminant diets. Asian-Aust. J. Anim. Sci. 16:211-216

Chanjula, P., M. Wanapat, C. Wachirapakorn, S. Uriyapongson and P. Rowlinson. 2004. Effect of synchronizing starch sources and protein (NPN) in the rumen on feed intake, rumen microbial fermentation, nutrient utilization and performance of lactating dairy cows. Asian-Aust. J. Anim. Sci. 17:1400-1410

Chen, X. B. and M. J. Gomes. 1992. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives-an overview of the technical details. International Feed Resources Unit, Rowel Research Institute, Aberdeen, UK

Cotta, M. A. and J. B. Russell. 1982. Effect of peptides and amino acids on efficiency of rumen bacteria protein synthesis in continuous culture. J. Dairy Sci. 65:226-234

Crocker, C. L. 1967. Rapid determination of urea nitrogen in serum or plasma without deproteinzation. Am. J. Medical Techn. 33:361-365

Erdman, R. A. 1988. Dietary buffering requirements of the lactating dairy cows. A review. J. Dairy Sci. 71:3246-3266

Fernandez, J. M., T. Sahulu, C. Lu, D. Ivey and M. J. Potchoiba. 1987. Production and metabolic aspects of non-protein nitrogen incorporation in lactation rations of dairy goats. Small Rum. Res. 26:105-107 crossref(new window)

France, J. and R. C. Siddons. 1993. Volatile fatty acid production. In: Quantilitive Aspects Ruminant Digestion and Metabolisim (Ed. J. M. Forbes and J. France). CAB International, Willingford, UK

Galyean, M. 1989. Laboratory Procedure in Animal Nutrition Research. Department of Animal and Life Science. New Mexico states University, USA

Giesecke, D., L. Ehrentrich and M. Stangassinger. 1994. Mammary and renal excretion of purine metabolites in relation to energy intake and milk yield in dairy cows. J. Dairy Sci. 77:2376-2381

Goering, H. K. and P. J. Van Soest. 1970. Forage Fiber Analysis (apparatus, reagent, procedures and some application). Agric. Handbook No. 379, ARS, USDA, Washington, DC

Gottschalk, G. 1986. Bateria metabolism (2nd Ed.). Sparinger- Verlag. New York

Hoover, W. H. 1986. Chemical factors involved in ruminal fiber digestion. J. Dairy Sci. 69:2755-2766

Hoover, W. H. and S. R. Stokes. 1991. Balancing carbohydrate and proteins for optimum rumen microbial yield. J. Dairy Sci. 74:3640-3644

Hungate, R. E. 1969. A roll tube method for cultivation of stric anaerobes. In: Methods in Microbiology. (Ed. J. R. Norris and D. W. Ribbons). New York

IAEA. 1997. Estimation of the rumen microbial protein production from purine derivatives in rumen. Animal production and healt section. Vienna, Austria

Khampa, S., M. Wanapat, C. Wachirapakorn, N. Nontaso and M. Wattiaux. 2006. Effect of levels of sodium dl-malate supplementation on ruminal fermentation efficiency in concentrates containing high levels of cassava chip in dairy steers. Aisan-Aust. J. Anim. Sci. (In Press)

Kim, Y. J., R. H. Liu, L. L. Rychlik and J. B. Russell. 2002. The enrichment of a ruminal bacterium (Megasphaere elsdenii Y J- 4) that produces the trans-10, cis-12 isomer of conjugated linoleic acid. J. Appl. Environ. Microbiol. 92:976-982 crossref(new window)

Kiyothong, K. and M. Wanapat. 2004. Growth, hay yield and chemical composition of cassava and Stylo 184 grown under intercropping. Aisan-Aust. J. Anim. Sci. 17:799-807

Lopez, S., C. Newbold and R. J. Wallace. 1999. Influence of sodium fumarate addition on rumen fermentation in vitro. Br. J. Nutr. 81:59-64

Martin, S. A., M. N. Streeter, D. J. Nisbet, G. M. Hill and E. E. Williams. 1999. Effect of DL-malate on ruminal metabolism and performance of cattle fed a high concentrate diets. J. Anim. Sci. 77:1008-1015

Newbold, C. J., R. J. Wallace and F. M. McIntosh. 1996. Mode of action of the yeast Saccharomyces cerevisiae as a feed additive for ruminants. Br. J. Nutr. 76:249-261 crossref(new window)

Owens, F. N., D. S. Secrist, W. J. Hill and D. R. Gill. 1998. Acidosis in cattle: a review. J. Anim. Sci. 76:275-286

Promkot, C. and M. Wanapat. 2005. Effect of level of crude protein and use of cottonseed meal in diets containg cassava chips and rice straw for lactating dairy cows. Asian-Aust. J. Anim. Sci. 18:502-511

Sanson, D. W. and O. T. Stallcup. 1984. Growth response and serum constituents of Holstein bulls fed malic acid. Nut. Rep. Int. 30:1261-1267

SAS. 1998. SAS/STAT User's Guide. Version 6.12. SAS Inst. Inc., Cary, NC, USA

Shingfield, K. J. and N. W. Offer. 1998. Determination of allantoin in bovine milk by high-performance liquid chromatography. J. Chromatogr. B. 706:342-346 crossref(new window)

Sommart, K., M. Wanapat, D. S. Parker and P. Rowlinson. 2000. Cassava chip as an energy source for lactating dairy cows fed rice straw. Asian-Aust. J. Anim. Sci. 13:1094-1101

Steel, R. G. D. and J. H. Torrie. 1980. Principles and Procedure of Statistics. McGraw-Hill Publishing Co., New York

Valadares, R. F. D., G. A. Broderick, S. C. Valadares Filho and M. K. Clayton. 1999. Effect of replacing alfalfa silage with high moisture corn on ruminal protein synthesis estimated from excretion of total purine derivatives. J. Dairy Sci. 82:2686-2696

Van Keulen, J. and B. A. Young. 1977. Evaluation of acid insoluble ash as a neutral marker in ruminant digestibility studies. J. Anim. Sci. 44:282-287

Wanapat, M. 1990. Nutritional Aspects of Ruminant Production in Southeast Asia with Special Reference to Thailand. Funny Press, Ltd., Bangkok, Thailand

Wanapat, M. 2000. Rumen manipulation to increase the efficient use of local feed resources and productivity of ruminants in the tropics. Asian-Aust. J. Anim. Sci. 13:59-67

Wanapat, M. 2003. Manipulation of cassava cultivation and utilization to improve protein to energy biomass for livestock feeding in the tropics. Asian-Aust. J. Anim. Sci. 16:463-472

Wanapat, M. and O. Pimpa. 1999. Effect of ruminal NH3-N levels on ruminal fermentation, purine derivatives, digestibility and rice straw intake in swamp buffaloes. Asian-Aust. J. Anim. Sci. 12:904-907

Wohlt, J. E., J. H. Clark and F. S. Blaisdell. 1978. Nutritional value of urea versus preformed protein for ruminants. II. Nitrogen utilization by dairy cows fed corn based diets containing supplemental nitrogen from urea and/or soybean meal. J. Dairy Sci. 61:916-931

Zinn, A. R. and F. N. Owen. 1986. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis. Can. J. Anim. Sci. 66:157-163