Advanced SearchSearch Tips
Role of Selenium in Alteration of Erythrocyte Parameters in Bovine Fluorosis
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Role of Selenium in Alteration of Erythrocyte Parameters in Bovine Fluorosis
Han, Bo; Yoon, Soon-Seek; Wu, Pei-Fu; Han, Hong-Ryul; Liang, Li-Cheng;
  PDF(new window)
Signs of dental discolouration, difficulty in mastication, bony exostosis and debility were observed in cattle from Qingtongxia Ningxia, China where fluoride concentration in drinking water, soil, fodder, serum, bone, teeth, haircoat and urine were significantly higher than the corresponding health site. The problem of fluorosis in beef cattle is attributable to water containing toxic levels of fluoride. The objective of this paper was therefore to evaluate the influence of fluoride on erythrocyte parameters in cattle under high fluoride and low selenium conditions, as well as the protective efficacy of selenium exposure in feedstuff for bovine endemic fluorosis. Sixteen 6 to 7 year-old high fluoride beef cattle were randomly allotted into two groups each with eight cows: high fluoride control group, and supplemented with 0.25 mg/kg selenium per day for 83 days respectively. In addition, eight 6 to 7 year-old normal control beef cattle were selected from a non-high fluoride site. Blood samples were collected on day 0, 30 and 83 for erythrocyte parameters analysis and scanning electronic microscopy. The results indicated that erythrocytes, hemoglobin, packed cell volume values and ATPase activity from affected cattle on the high fluoride site were significantly reduced during the period as compared with the corresponding samples of normal control cattle, a great number of echinocytes were present in peripheral blood, and subsequent anaemia. However, affected cattle exposed to selenium revealed increasable erythrocyte parameters, the extent of elevation in these values being dependent on the duration of supplementation with selenium. These findings suggest that fluoride exposure can cause erythrocyte damage, whereas selenium supplementation can antagonize fluoride-induced generation of free radicals and cumulative effects of lipid peroxidation in erythrocytes. Selenium supplementation may help to alleviate the possible hazards associated with bovine endemic fluorosis.
Cattle;Erythrocyte Parameters;Endemic Fluorosis;Selenium;
 Cited by
Bildik, A., F. Belge, F. Yur, M. Alkan and D. Kilicalp. 2002. The effect of hyperthyroidism on the levels of $Na^+-K^+$ ATPase, G6PD and glutathione. Israel J. Vet. Med. 57:19-22

Bober, J., D. Chlubek, E. Kwiatkowska, K. Kędzierska, E. Stachowska, P. Wieczorek, E. Byra, Z. Machoy and E. Herdzik. 2001. Influence of fluoride ions on $Na^+-H^+$ exchanger activity in human red blood cells. 34(3):174-180

Cernik, A. A., J. A. Cooke and R. J. Hall. 1970. Specific ion electrode in the determination of urinary fluoride. Nat. 227:

Cronin, S. J. and D. S. Sharp. 2002. Environmental impacts on health from continuous volcanic activity at Yasur (Tanna) and Ambrym, Vanuatu. Int. J. Environ. Health Res. 12(2):109-123 crossref(new window)

Dwivedi, S. K., S. Dey and D. Swarup. 1997. Hydrofluorosis in water buffalo (Bubalus bubalis) in India. The Science of the Total Environment. 207:105-109 crossref(new window)

Guan, Z. Z., K. Q. Xiao, X. Y. Zeng, Y. G. Long, Y. H. Cheng, S. F. Jiang and Y. N. Wang. 2000. Changed cellular membrane lipid composition and lipid peroxidation of kidney in rats with chronic fluorosis. Archiv. Toxicol. 74(10):602-608 crossref(new window)

Guven, A. and N. Kaya. 2005. Effect of fluoride intoxication on lipid peroxidation and reduced glutathione in Tuj sheep. Fluoride. 38(2):139-142

Han, B., D. B. Zhong, P. F. Wu, W. J. Qu and M. Wang. 2004. Effect of excessive fluoride environment on productivity and reproductive performance in dairy cows, XXIII World Buiatrics Congress, Quebec, Canada, 7

Han, B., M. Y. Li and Y. Shi. 2002. Studies on the toxicology of endemic fluorosis in cattle. XXII World Buiatrics Congress. Hannover. German. 8

Han, B., S. S. Yoon, J. L. Su, H. R. Han, M. Wang, W. J. Qu and D. B. Zhong. 2004. Effects of selenium, copper and magnesium on antioxidant enzymes and lipid peroxidation in bovine fluorosis, Asian-Aust. J. Anim. Sci. 17(12):1695-1699

Inkielewicz, I. and J. Krechniak. 2004. Fluoride effects on glutathione peroxidase and lipid peroxidation in rats. Fluoride, 37(1):7-12

ISI- method for determination of quality of milled rice IS: 10404- 1983. Indian Standards Institution, Manak Bhavan, 9, Bahadur Shah Zafar Marg, New Delhi

Jubb, T. F., T. E. Annand, D. C. Main and G. M. Murphy. 1993. Phosphorus supplements and fluorosis in cattle: a Northern Australian Experience. Aust. Vet. J. 70(10):379-383 crossref(new window)

Loganathan, P., M. J. Hedley, G. C. Wallaceand and A. H. C. Roberts. 2001. Fluoride accumulation in pasture forages and soils following long-term applications of phosphorus fertilizers. Environmental Pollution. 115:275-282 crossref(new window)

McDowell, L. R. 1992. Minerals in animal and human nutrition. London: Academic Press. 333-351

Mehdi, A. W., M. T. Ridha, A. A. Al-Kafawi and M. H. Injidi. 1978. Effect of high fluoride intake on haematological aspects of the mouse. Q. J. Exp. Physiol. Cogn. Med. Sci. 63(1):83-88

Patra, R. C., S. K. Dwivedi, B. Bhardwaj and D. Swarup. 2000. Industrial fluorosis in cattle and buffalo around Udaipur, India. Sci. Total. Environ. 253(1-3):145-150 crossref(new window)

Radostitis, O. M., C. C. Gay, D. C. Blood and K. W. Hinchcliff. 2000. Veterinary Medicine (9th Edition). WB Saunders, Philadelphia. 1489-1493

Sahoo, N. and S. K. Ray. 1998. Monitoring of fluoride content in the environment around an aluminium smelter. Toxicol. Lett. 95:231-232

SAS. 2000. SAS/STAT User's Guide: Statistics. Version 8th Edition. SAS Institute Inc., Cary. North Carolina

Serpersu, E. and G. Ciliv. 1978. Some properties of Na-K dependent adenosine triphosphate from human erythrocyte. Biochem. Med. 20:31-39 crossref(new window)

Shivashankara, A. R., Y. M. Shivarajashankara, P. G. Bhat and R. S. Hanumantha. 2002. Lipid peroxidation and antioxidant defense systems in liver of rats in chronic fluoride toxicity. Bull. Environ. Contam. Toxicol. 68(4):612-616 crossref(new window)

Shupe, J. L., A. E. Olson and R. P. Sharma. 1972. Fluoride toxicity in domestic and wild animals. Clin. Toxicol. 5(2):195-213 crossref(new window)

Susheela, A. K. and S. K. Jain. 1983. Fluoride-induced haematological changes in rabbits. Bull. Environ. Contamin. Toxicol. 30(4):388-393 crossref(new window)

Thompson, D. J. 1978. In: (Ed. J. H. Conrad and L. R. McDowell). Proceedings of the Latin american symposium on mineral nutrition research with grazing ruminants. Univ. Fluorida, Gainsville, Florida. 47-73

Wang, A. G., T. Xia, R. Ru, J. Yuan, X. M. Chen and K. D. Yang. 2004. Antagonistic effect of selenium on oxidative stress, DNA damage, and apoptosis induced by fluoride in human hepatocytes. Fluoride. 37(2):107-116

Wei, Z. D. and Y. Wei. 2000. Fluoridation in China: A clouded future. Fluoride. 35(1):1-4

Yur, F., F. Belge, N. Mert and I. Yoruk. 2003. Changes in erthyrocytes parameters of fluorotic sheep. Fluoride. 36(3):152-156