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Metabolic Elasticity and Induction of Heat Shock Protein 70 in Labeo rohita Acclimated to Three Temperatures
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Metabolic Elasticity and Induction of Heat Shock Protein 70 in Labeo rohita Acclimated to Three Temperatures
Das, T.; Pal, A.K.; Chakraborty, S.K.; Manush, S.M.; Chatterjee, N.; Apte, S.K.;
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The metabolic response of Labeo rohita to thermal acclimation was assessed. Advanced fingerlings of L. rohita (average weight ) were acclimated to 31, 33 and compared with ambient temperatures () for 30 days and different enzymes associated with stress response were estimated. Glycolytic enzyme-Lactate dehydrogenase, (LDH, E.C., TCA cycle enzyme-Malate dehydrogenase (MDH, E.C., Protein metabolizing enzymes-Aspartate amino transferase (AST, E.C. and Alanine amino transferase (ALT, E.C. of liver, gill and muscle, Gluconeogenic enzymes-Fructose 1,6 Bi phosphatase (FBPase, E.C. and Glucose 6 phosphatase (G6Pase, E.C. of liver and kidney were significantly (p<0.05) different with increasing acclimation temperatures. Heat Shock Protein-70 (HSP-70) was expressed in increasing intensity at 31, 33 and but was not expressed at . Results suggest that higher acclimation temperatures enhance metabolism and L. rohita maintains homeostasis between via an acclimation episode. Such adaptation appears to be facilitated by resorting to gluconeogenic and glycogenolytic pathways for energy mobilization and induction of HSPs.
Thermal Acclimation;Labeo rohita;Metabolic Activities;Heat Shock Protein 70;
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Baroudy, E. and J. M. Elliott. 1994. Tolerance of parr of Arctic charr, Salvelinus alpinus to reduce dissolved oxygen concentrations. J. Fish. Biol. 44:736-738 crossref(new window)

Beitinger, T. L., W. A. Bennett and R. W. McCauley. 2000. Temperature tolerances of North American freshwater fishes exposed to dynamic changes in temperature. Env. Biol. Fish. 58: 237-275 crossref(new window)

Bennett, W. A. and T. L. Beitinger. 1997. Temperature tolerance of the sheepshead minnow, Cyprinodon variegates. Copeia. 1997:77-87

Blatter, D. P., F. Garner, K. Van Slyke and A. Bradley. 1972. Quantitative electrophoresis in polyacrylamide gels of 2-40%. J. Chromato. 64:147-155 crossref(new window)

Chatterjee, N., A. K. Pal, S. M. Manush, T. Das and S. C. Mukherjee. 2004. Thermal tolerance and metabolic status of Labeo rohita and Cyprinus carpio early fingerlings acclimated to three different temperatures. J. Therm. Biol. 29:265-270 crossref(new window)

Chung, M. K., J. H. Choi, Y. K. Chung and K. M. Chee. 2005. Effects of dietary vitamins C and e on eggshell quality of broiler breeder hens exposed to heat stress. Asian-Aust. J. Anim. Sci. 18(4):545-551 crossref(new window)

Currie, S., C. D. Moyes and B. L. Tufts. 2000. The effects of heat shock and acclimation temperature on HSP70 and HSP30 mRNA expression in rainbow trout: in vivo and in vitro comparisons. J. Fish. Biol. 56:398-408 crossref(new window)

Das, T., A. K. Pal, S. K. Chakraborty, S. M. Manush, N. Chatterjee and S. C. Mukherjee. 2004. Thermal tolerance and oxygen consumption of Indian Major Carps acclimated to four different temperatures. J. Therm. Biol. 29:157-163 crossref(new window)

Das, T., A. K. Pal, S. K. Chakraborty, S. M. Manush, N. P. Sahu and S. C. Mukherjee. 2005. Thermal tolerance, growth and oxygen consumption of Labeo rohita fry (Hamilton, 1822) acclimated to four temperatures Journal of Thermal Biology. 30(5):378-383 crossref(new window)

Dietz, T. J. and G. N. Somero. 1992. The threshold induction temperature of the 90 kDa heat shock protein is subject to acclimatization in eurythermal goby fishes (Gillichhys). In proceedings of National Academy of Science. USA. 89:3389- 3393

Feder, M. E. and G. E. Hofmann. 1999. Heat shock proteins, molecular chaperons, and the stress response: Evolutionary and ecological physiology. Annu. Rev. Physiol. 61:243-282 crossref(new window)

Feige, U., R. Morimoto, I. Yahara and B. S. Polla. 1996. Stressinducible cellular responses Birkhauser-Verlag. Basel, Switzerland, p. 492

Forsyth, R. B., E. P. M. Candido, S. L. Babich and G. K. Iwama. 1997. Stress protein expression in Coho salmon with bacterial kidney disease. J. Aqua. Anim. Health. 9:18-25 crossref(new window)

Freeland, R. A. and A. L. Harper. 1959. The study of metabolic pathway by means of adaptation. J. Biol. Chem. 234:1350-1354

Guderley, H. 1990. Functional significance of metabolic responses to thermal acclimation in fish muscle. Am. J. Physiol. 259:245-252

Hayford, Y. T., K. Sato, K. Takahashi, M. Toyomizu and Y. Akiba. 2002. Effects of Heat Stress and Dietary Tryptophan on Performance and Plasma Amino Acid Concentrations of Broiler Chickens. Asian-Aust. J. Anim. Sci. 15(2):247-253 crossref(new window)

Hutchinson, K. A., K. D. Ditmar, M. J. Czar and W. B. Pratt. 1994. Proof that HSP70 is required for assembly of glucocorticoid receptor into a heterocomplex with HSP90. J. Biol. Chem. 269:5043-5049

Iwama, G. K., P. T. Thomas, R. B. Forsyth and M. M. Vijayan. 1998. Heat shock protein expression in fish. Rev. Fish Biol. Fish. 8:1-22 crossref(new window)

Iwama, G. K., M. M. Vijayan, R. B. Forsyth and P. A. Ackerman. 1999. Heat shock proteins and physiological stress in fish. Am. Zool. 39:901-909 crossref(new window)

Kita, J., S. Tsuchida and T. Setoguma. 1996. Temperature preferance and tolerance, and oxygen consumption of the marbled rock-fish, Sebastiscus marmoratus. Mar. Biol. 125:467-471

Kutty, M. N. 1981. Energy metabolism in mullet. In: Aquaculture of grey mullets. (Ed. O. H. Oven). pp. Cambridge University Press, London, pp. 219-253

Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680-685 crossref(new window)

Lowry, O. H., N. J. Ronebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with Folin Phenol Reagent. J. Biol. Chem. 193:265-276

Manush, S. M., A. K. Pal, N. Chatterjee, T. Das and S. C. Mukherjee. 2004. Thermal tolerance and oxygen consumption of Macrobrachium rosenbergii acclimated to three temperatures. J. Therm. Biol. 29:15-19 crossref(new window)

Marjoic, A. S. 1964. Methods in Enzymology. Vol. II. (S. P. Colowick and N. O. Kaplan). Academic Press Inc, New York, p. 541

Milligan, C. L. and S. S. Girard. 1993. Lactate metabolism in rainbow trout. J. Experi. Biol. 180:175-193

Minier, C., V. Borghi, M. N. Moore and C. Porte. 2000. Seasonal variation of MXR and stress proteins in the common mussels, Mytillus galloprovincialis. Aquat. Toxicol. 50:167-176 crossref(new window)

Ming, Y. C., S. Y. Huang, E. C. Lin, T. H. Hseu and W. C. Lee. 2003. Association of a Single Nucleotide Polymorphism in the 5'-Flanking Region of Porcine HSP70.2 with Backfat Thickness in Duroc Breed. Asian-Aust. J. Anim. Sci. 16(1):100-103 crossref(new window)

Moon, T. W. and G. D. Foster. 1995. Tissue carbohydrate metabolism, gluconeogenesis and hormonal and environmental influences. In: Metabolic and Adaptational Biochemistry. (Ed. P. W. Hochachka and T. P. Mommsen). Amsterdam, Elsevier Science, pp. 65-100

Ochoa, 1955. Malic dehydrogenase and 'malic' enzyme. In: Methods of enzymology (Ed. I. S. P. Coloric and Kaplan). Academic Press, New York, pp. 735-745

Pal, A. K. and S. C. Mukherjee. 2003. Heat shock Protein Expression in Fish and Shellfish: Environmental Perspectives and Health Management. In: Biotechnology in Environemntal Management. (T. Chakrabrti, T. K. Ghosh and G. Tripathi). APH Publishers, New Delhi, pp. 95-116

Palmisano, N. A., J. R. Winton and W. W. Dickhoff. 2000. Tissuesspecific induction of HSP 90 mRNA and plasma cortisol response in Chinook Salmon following heat shock, sea water challenge and handling challenge. Mar. Biotechnol. 2:329-338

Paromita, D., G. Akhil and K. M. Sanjib. 2005. Heat shock protein 70 expression in different tissues of Cirrhinus mrigala (Ham.) following heat stress. Agric. Res. 36(6):525-529

Renukardhyay, K. M. and T. J. Varghese. 1986. Protein requirement of the carps Catla catla and Labeo rohita (Ham.). Proc. Indian Acad. Sci. (Anim. Sci.), 95:103-107

Sanders, B. M. 1993. Stress proteins in aquatic organisms: an environmental perspective. Crit. Rev. Toxicol. 23:49-75 crossref(new window)

Schlesinger, M. J., M. Ashburner and Tissieres. 1982. Heat Shock Proteins from Bacteria to Man, Cold Springe Harbor Lab Press, NY. pp. 243-251

Suarez, R. K. and T. P. Mommsen. 1987. Gluconeogenesis in teleost fishes. Can. J. Zool. 65:1869-1882 crossref(new window)

Sudarman, A. and T. Ito. 2000. Heat Production and Thermoregulatory Responses of Sheep Fed Different Roughage Proportion Diets and Intake Levels When Exposed to a High Ambient Temperature. Asian-Aust. J. Anim. Sci. 13(5):625-629 crossref(new window)

Towbin, H., T. Staehelin and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceed. Nat. Acad. Sci. USA. 76(9):4350-4354

Vijayan, M. M., J. S. Ballantyne and J. F. Leatherland. 1990. High stocking density alters the energy metabolism of brook charr, Salvelinus fontinalis. Aquac. 88:371-381 crossref(new window)

Vijayan, M. M., C. Pereira, E. G. Grau and G. K. Iwama. 1997. Metabolic responses associated with confinement stress in tilapia: the role of cortisol. Comp. Biochem. Physiol. 116C:89-95

Wedemeyer, G. R., F. P. Meyer and L. Smith. 1999. Environmental stress and Fish diseases. Narendra Publ. House, Delhi, India, p. 107

Werner, I., C. S. Koger, J. T. Hamm and D. E. Hinton. 2001. Ontogeny of the Heat Shock Protein, HSP70 and HSP 60, Response and Development effects of Heat- Shock in the Teleost, Medaka (Oryzias latipes). Environ. Sci. 8:13-29

Wotton, I. D. P. 1964. Microanalysis. In Medical Biochemistry. J. A. Churchill (4th Eds).London, pp. 101-107

Wrobleuiski, L. and J. S. Ladue. 1955. LDH activity in blood. Proc. Soc. Exp. Biol. Med. 90:210-213

Zulkifli, I., S. A. Mysahra and L. Z. Jin. 2004. Dietary supplementation of betaine (betafin) and response to high temperature stress in male broiler chickens. Asian-Aust. J. Anim. Sci. 17(2):244-249 crossref(new window)