Advanced SearchSearch Tips
Ginsenosides Promote Proliferation of Cultured Ovarian Germ Cells Involving Protein Kinase C-mediated System in Embryonic Chickens
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Ginsenosides Promote Proliferation of Cultured Ovarian Germ Cells Involving Protein Kinase C-mediated System in Embryonic Chickens
Liu, Hongyun; Zhang, Caiqiao;
  PDF(new window)
The effect of ginsenosides (GS) on germ cell proliferation was evaluated with a chicken ovarian germ-somatic cell coculture model and the mechanism involving protein kinase C (PKC) pathway was investigated. Ovarian cells were cultured in serum-free McCoy's 5A medium and challenged with GS alone or in combinations with PKC activator (phorbol 12-myristate 13-acetate, PMA) or inhibitor () for 48 h. The number of germ cells was counted and the proliferating cells were identified by immunocytochemistry of proliferating cell nuclear antigen (PCNA). Results showed that GS significantly increased germ cell proliferation and this stimulating effect was further increased by PMA, but inhibited by H7, in a dose-dependent manner. Moreover, GS-elevated PCNA expression and the PCNA -labeling index of germ cells displayed similar changes with the increased numbers of germ cells. These results indicated that GS stimulated proliferation of ovarian germ cells with involvement of the PKC-mediated system.
Ginsenosides;Protein Kinase C;Germ Cell;Proliferating Cell Nuclear Antigen;Chicken;Ovary;
 Cited by
Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C, Journal of Zhejiang University SCIENCE B, 2009, 10, 2, 87  crossref(new windwow)
Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation, Journal of Zhejiang University SCIENCE B, 2010, 11, 12, 952  crossref(new windwow)
and Rd Regulate Proliferation of Mature Keratinocytes Through Induction of p63 Expression in Hair Follicles, Phytotherapy Research, 2012, 27, 7, 1095  crossref(new windwow)
Chan, L., P. Y. Chiu and T. K. Lau. 2003. An in-vitro study of ginsenoside Rb1-induced teratogenicity using a whole rat embryo culture model. Hum. Reprod. 18:2166-2168 crossref(new window)

Choi, S., H. J. Kim, Y. S. Ko, S. W. Jeong, Y. I. Kim, W. F. Simonds, J. W. Oh and S. Y. Nah. 2001. $G_{\alpha}_{q/11}$ coupled to mammalian phospholipase C $\beta$3-like enzyme mediates the ginsenoside effect on $Ca^{2+}$-activated $Cl^{-}$ current in the Xenopus oocyte. J. Biol. Chem. 276:48797-48802 crossref(new window)

Choi, S., J. H. Lee, Y. I. Kim, M. J. Kang, H. Rhim, S. M. Lee and S. Y. Nah. 2003. Effects of ginsenoside on G protein-coupled inwardly rectifying $K^{+}$ channel activity expressed in Xenopus oocytes. Eur. J. Pharmacol. 468:83-92 crossref(new window)

Denli, M., S. Cankaya, S. Silici, F. Okan and A. N.Uluocak. 2005. Effect of dietary of turkish propolis on the growth performance, carcass characteristics and serum variables of quail (Coturnix coturnix japonica). Asian-Aust. J. Anim. Sci. 18:848-854

Kim, D. H., Y. S. Moon, T. H. Lee, J. S. Jung, H. W. Suh and D. K. Song. 2003. The inhibitory effect of ginseng saponins on the stress-induced plasma interleukin-6 level in mice. Neurosci. Lett. 353:13-16 crossref(new window)

Lee, E., E. Ko, J. Lee, S. Rho, S. Ko, M. K Shin, B. Min, M. C. Hong, S. Kim and H. Bae. 2004. Ginsenoside Rg1 enhances $CD4^{+}$ T-cell activities and modulates Th1/Th2 differentiation. Int. Immunopharmacol. 4:235-244 crossref(new window)

Li, Z., X. Chen, Y. Niwa, S. Sakamoto and Y. Nakaya. 2001. Involvement of $Ca_{2+}$-activated $K_{+}$ channels in ginsenosidesinduced aortic relaxation in rats. J. Cardiovasc. Pharmacol. 37:41-47 crossref(new window)

Li, Z., Y. Niwa, S. Sakamoto, M. Shono, X. Chen and Y. Nakaya. 2000. Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells. Life Sci. 67:2983-2989 crossref(new window)

Liu, H., C. Zhang, X. Tang, W. Zeng and Y. Mi. 2005. Stimulating effects of androgen on proliferation of cultured ovarian germ cells through androgenic and estrogenic actions in embryonic chickens. Domest. Anim. Endocrinol. 28:451-462 crossref(new window)

Liu, W., S. Xu and C. Che. 2000. Anti-proliferative effect of ginseng saponins on human prostate cancer cell line. Life Sci. 67:1297-1306 crossref(new window)

Mizumaki, Y., M. Kurimoto, Y. Hirashima, M. Nishijima, H. Kamiyama, S. Nagai, A. Takaku, K. Sugihara, M. Shimizu and S. Endo. 2002. L ipophilic fraction of Panax ginseng induces neuronal differentiation of PC12 cells and promotes neuronal survival of rat cortical neurons by protein kinase C dependent manner. Brain Res. 950:254-260 crossref(new window)

Nah, S. Y., H. J. Park and E. W. McCleskey. 1995. A trace component of ginseng that inhibits $Ca_{2+}$ channels through a pertussis toxin-sensitive G protein. Proc. Natl. Acad. Sci. USA. 92:8739-8743

Odani, T., H. Tanizawa and Y. Takino. 1983. Studies on the absorption, distribution, excretion and metabolism of ginseng saponins. II. The absorption, distribution and excretion of ginsenoside Rg1 in the rat. Chem. Pharm. Bull. 31:292-298

Ohtani, K., H. Sakamoto, A. Kikuchi, Y. Nakayama, T. Idei, N. Igarashi, T. Matukawa and K. Satoh. 2001. Follicle-stimulating hormone promotes the growth of human epithelial ovarian cancer cells through the protein kinase C-mediated system. Cancer Lett. 166:207-213 crossref(new window)

Popovich, D. G. and D. D. Kitts. 2002. Structure-function relationship exists for ginsenosides in reducing cell proliferation and inducing apoptosis in the human leukemia (THP-1) cell line. Arch. Biochem. Biophys. 406:1-8 crossref(new window)

Radad, K., G. Gille, R. Moldzio, H. Saito and W. D. Rausch. 2004. Ginsenosides Rb1 and Rg1 effects on mesencephalic dopaminergic cells stressed with glutamate. Brain Res. 1021:41-53 crossref(new window)

Santos, A. A. and E. G. Araujo. 2000. The effect of PKC activation on the survival of rat retinal ganglion cells in culture. Brain Res. 853:338-343 crossref(new window)

Takino, Y., T. Odani, H. Tanizawa and T. Hayashi. 1982. Studies on the absorption, distribution, excretion and metabolism of ginseng saponins. I. Quantitative analysis of ginsenoside Rg1 in rats. Chem. Pharm. Bull. 30:2196-2201

Tian, J., F. Fu, M. Geng, Y. Jiang, J. Yang, W. Jiang, C. Wang and K. Liu. 2005. Neuroprotective effect of 20(S)-ginsenoside Rg3 on cerebral ischemia in rats. Neurosci. Lett. 374:92-97 crossref(new window)

Wang, B., J. Liu and L. Zhu. 2005. Effect of compatibility on the pharmacokinetic characteristics of ginsenosides. Colloids Surf. B: Biointerfaces. 41:219-222 crossref(new window)

Wang, M., L. J. Guilbert, J. Li, Y. Wu, P. Pang, T. K. Basu and J. J. Shan. 2004. A proprietary extract from North American ginseng (Panax quinquefolium) enhances IL-2 and IFN-$\gamma$ productions in murine spleen cells induced by Con-A. Int. Immunopharmacol. 4:311-315 crossref(new window)

Xu, Y., J. Shi and Z. Jiang. 2005. Inhibitory influence of ginsenoside Rb3 on activation of strychnine-sensitive glycine receptors in hippocampal neurons of rat. Brain Res. 1037:99-106 crossref(new window)

Yu, W. J., B. J. Lee, S. Y. Nam, D. C. Yang and Y. W. Yun. 2003. Modulating effects of Korean ginseng saponins on ovarian function in immature rats. Biol. Pharmacol. Bull. 26:1574-1580 crossref(new window)