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Milk Protein Production and Plasma 3-Methylhistidine Concentration in Lactating Holstein Cows Exposed to High Ambient Temperatures
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 Title & Authors
Milk Protein Production and Plasma 3-Methylhistidine Concentration in Lactating Holstein Cows Exposed to High Ambient Temperatures
Kamiya, Mitsuru; Kamiya, Yuko; Tanaka, Masahito; Shioya, Shigeru;
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This experiment was performed to examine the influences of high ambient temperature on milk production, nutrient digestibility, energy and protein sufficiency ratio, and plasma metabolites concentration in lactating cows. In a crossover design, four multiparous lactating Holstein cows were maintained in a chamber under treatment of constant moderate () ambient temperature (MT) or high () ambient temperatures (HT). The DMI and milk protein yield were significantly lower in HT (p<0.05). The milk yield, milk lactose yield, and milk SNF yield tended to be lower in HT (p<0.10). No statistical differences for 4% fat-corrected milk and milk fat yield were observed. Rectal temperatures were significantly higher in HT than MT (p<0.05). The apparent DM, OM, ether extract, CF, and ash digestibility did not differ between treatments. On the other hand, the apparent CP digestibility was increased significantly (p<0.05) and nitrogen free extract tended to increase (p<0.10) in HT. The sufficiency ratio of ME and DCP intake for each requirement tended to be lower in HT than in MT (p<0.10). Concentrations of total protein (TP), albumin, and urea nitrogen in plasma did not differ between treatments. Plasma 3-methylhistidine (3MH) concentration as a marker of myofibrillar protein degradation tended to be higher in HT (p<0.15). In conclusion, high ambient temperature was associated with a lower energy and protein sufficiency ratio, and decreased milk protein production, even though the body protein mobilization tended to be higher.
Dairy Cows;Heat Stress;Milk Production;Energy;Protein;Protein Degradation;
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AOAC. 1990. Official Methods of Analysis. 15th edn. Association of Official Analytical Chemists, Arlington, Virginia

Baracos, V. E., E. J. Wilson and A. L. Goldberg. 1984. Effects of temperature on protein turnover in isolated rat skeletal muscle. Am. J. Physiol. 246:C125-C130 crossref(new window)

Bernabucci, U., P. Bani, B. Ronchi, N. Lacetera and A. Nardone. 1998. Influence of short- and long-term exposure to a hot environment on rumen passage rate and diet digestibility by Friesian heifers. J. Dairy Sci. 82:967-973 crossref(new window)

Bernabucci, U., N. Lacetera, B. Ronchi and A. Nardone. 2002. Effects of the hot season on milk protein fractions in Holstein cows. Anim. Res. 51:25-33 crossref(new window)

Blum, J. W., T. Reding, F. Jans, M. Wanner, M. Zemp and K. Bachmann. 1985. Variations of 3-methylhistidine in blood of dairy cows. J. Dairy Sci. 68:2580-2587 crossref(new window)

Broderick, G. A. and M. K. Clayton. 1997. A statistical evaluation of animal and nutritional factors influencing concentrations of milk urea nitrogen. J. Dairy Sci. 80:2964-2971 crossref(new window)

Bunting, L. D., J. M. Fernandez, R. J. Fornea, T. W. White, M. A. Froetschel, J. D. Stone and K. Ingawa. 1996. Seasonal effects of supplemental fat or undegradable protein on the growth and metabolism of Holstein calves. J. Dairy Sci. 79:1611-1620 crossref(new window)

Emery, R. S. 1978. Feeding for increased milk protein. J. Dairy Sci. 61:825-828 crossref(new window)

Hirayama, T. and K. Katoh. 2004. Effects of heat exposure and restricted feeding on behavior, digestibility and growth hormone secretion in goats. Asian-Aust. J. Anim. Sci. 17:655-658 crossref(new window)

Hirayama, T., K. Katoh and Y. Obara. 2004. Effects of heat exposure on nutrient digestibility, rumen contraction and hormone secretion in goats. Anim. Sci. J. 75:237-243 crossref(new window)

Itoh, M. and R. Tano. 1977. Determination of the heat of combustion in fresh feces and urine with polyethylene film. Bulletin of the National Institute of Animal Industry 32:39-43

Kamiya, M., Y. Iwama, M. Tanaka and S. Shioya. 2005. Effects of high ambient temperature and restricted feed intake on nitrogen utilization for milk production in lactating dairy cows. Anim. Sci. J. 76:217-223 crossref(new window)

Komaragiri, M. V. and R. A. Erdman. 1997. Factors affecting body tissue mobilization in early lactation dairy cows: 1. Effect of dietary protein on mobilization of body fat and protein. J. Dairy Sci. 80:929-937 crossref(new window)

Kurihara, M., A. Mukai and M. Shibata. 1989. Energy metabolism of dairy cattle under high environmental temperature: 2. Performance tests of open circuit respiration apparatus. Bulletin of the Kyushu National Agricultural Experiment Station 26:71-88

Lee, J. H., C. K. Kim, Y. C. Chung, C. H. Kim and J. T. Yoon. 2004. Effects of milk production, season, parity and lactation period on variations of milk urea nitrogen concentration and milk components of Holstein dairy cows. Asian-Aust. J. Anim. Sci. 17:479-484 crossref(new window)

MAFF. 1999. Japanese Feeding Standard for Dairy Cattle. Japan Livestock Industry Association, Tokyo

Mazumder, M. A. R. and H. Kumagai. 2006. Analyses of factors affecting dry matter intake of lactating dairy cows. Anim. Sci. J. 77:53-62 crossref(new window)

Mukai, A., M. Shibata and M. Kurihara. 1989. Energy metabolism of dairy cattle under high environmental temperature: 1. Description of the energy metabolism laboratory at the Kyushu National Agricultural Experiment Station. Bulletin of the Kyushu National Agricultural Experiment Station 26:27-69

Muroya, S., F. Terada and S. Shioya. 1997. Influence of heat stress on distribution of nitrogen in milk. Anim. Sci. Technol. (Jpn.) 68:297-300

Nagasawa, T., J. Hirano, F. Yoshizawa and N. Nishizawa. 1998. Myofibrillar protein catabolism is rapidly suppressed following protein feeding. Biosci. Biotechnol. Biochem. 62:1932-1937 crossref(new window)

Nagasawa, T., F. Yoshizawa and N. Nishizawa. 1996. Plasma $N^\tau$-methylhistidine concentration is a sensitive index of myofibrillar protein degradation during starvation in rats. Biosci. Biotechnol. Biochem. 60:501-502 crossref(new window)

Nakashima, K., I. Nonaka, S. Masaki, M. Yamazaki and H. Abe. 2004. Myofibrillar proteolysis in chick muscle cell cultures during heat stress. Anim. Sci. J. 75:353-360 crossref(new window)

National Agricultural Research Organization. 2001. Standard Tables of Feed Composition in Japan. Japan Livestock Industry Association, Tokyo

Ndibualonji, B. B., D. Dehareng, F. Beckers, C. Van Eenaeme and J.-M. Godeau. 1997. Continuous profiles and within-day variations of metabolites and hormones in cows fed diets varying in alimentary supplies before short-term feed deprivation. J. Anim. Sci. 75:3262-3277

Oltner, R. and H. Wiktorsson. 1983. Urea concentrations in milk and blood as influenced by feeding various amounts of protein and energy to dairy cows. Livest. Prod. Sci. 10:457-467 crossref(new window)

SAS Institute Inc. 1999. SAS/STAT User's Guide: Version 8. SAS Institute Inc., Cary, North Carolina

Shibata, M. 1983. Thermal balance and lactation of dairy cattle in a hot environment. Jpn. J. Zootech. Sci. 54:635-647

Terada, F. and S. Shioya. 1998. Effects of fish meal supplementation and environmental conditions on nitrogen excretion in lactating cows. Anim. Sci. Technol. (Jpn.) 69:620-624

Thompson, M. G., R. M. Palmer, A. Thom, S. C. Mackie, K. S. Morrison and C. I. Harris. 1996. Measurement of protein degradation by release of labeled 3-methylhistidine from skeletal muscle and non-muscle cells. J. Cell. Physiol. 166:506-511 crossref(new window)

Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy. Sci. 74:3583-3597 crossref(new window)

Wassner, S. J., J. L. Schlitzer and J. B. Li. 1980. A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography. Anal. Biochem. 104:284-289 crossref(new window)

Yoshizawa, F., T. Nagasawa, N. Nishizawa and R. Funabiki. 1997. Protein synthesis and degradation change rapidly in response to food intake in muscle of food-deprived mice. J. Nutr. 127:1156-1159

Young, V. R., S. D. Alexis, B. S. Baliga, H. N. Munro and W. Muecke. 1972. Metabolism of administered 3-methylhistidine. Lack of muscle transfer ribonucleic acid charging and quantitative excretion as 3-methylhistidine and its N-acetyl derivative. J. Biol. Chem. 247:3592-3600