Advanced SearchSearch Tips
Effect of Supplementing 2-Hydroxy-4-(Methylthio) Butanoic Acid and DL-methionine in Corn-soybean-cottonseed Meal Diets on Growth Performance and Carcass Quality of Broilers
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Supplementing 2-Hydroxy-4-(Methylthio) Butanoic Acid and DL-methionine in Corn-soybean-cottonseed Meal Diets on Growth Performance and Carcass Quality of Broilers
Liu, Y.L.; Song, G.L.; Yi, G.F.; Hou, Y.Q.; Huang, J.W.; Vazquez-Anon, M.; Knight, C.D.;
  PDF(new window)
This experiment was conducted to compare the effects of feeding DL-2-hydroxy-4-(methylthio)butanoic acid (HMTBA) and DL-methionine (DLM) supplemented corn-soybean-cottonseed meal diets on growth performance, carcass composition, and muscle color of broilers. The trial was designed as a factorial experiment, including two methionine (Met) sources (HMTBA and DLM), three equimolar graded levels of Met supplementation (i.e., 0.08, 0.16, and 0.24% in the starter diet and 0.07, 0.14, and 0.21% in the grower and finisher diets, respectively), and two sexes (male and female). Additionally, one basal diet for each sex was formulated to be limiting in Met to test the dosage response of increasing supplemental Met levels. Four hundred and twenty 10-d-old broilers were randomly allotted to 14 treatments (seven each for males and females), with five replicate pens per treatment and six chicks per pen. There was no difference (p>0.05) between the two Met sources in growth performance and muscle deposition of broilers throughout the whole experimental period (d 10 to 49). With the increasing Met supplementation levels, average daily gain was increased (quadratic; p<0.01) during the starter, grower, and overall phases, average daily feed intake was increased (quadratic; p<0.01) during the starter phase, and feed:gain ratio was decreased (quadratic; p<0.05) during the grower and overall phases. At the end of finisher phase, Met supplementation increased breast muscle content (quadratic; p<0.01) and thigh muscle content (linear; p<0.05), and decreased abdominal fat content (quadratic; p<0.02). Compared to the broiler fed DLM, broilers fed HMTBA had superior breast and thigh muscle coloration (p<0.01). Male broilers had higher weight gain and feed intake and better feed conversion than female broilers (p<0.01). The fat content of thigh muscle in female broilers was higher than that of male broilers (p<0.03). The best fit comparison of HMTBA vs. DLM was determined by Schwarz Bayesian Criteria index, which indicated that the average relative bioefficacy of HMTBA vs. DLM was 120% with 95% confidence limit 67 to 172%. These results indicated that Met supplementation improved growth performance and carcass quality of broilers fed corn-soybean-cottonseed meal diets irrespective of Met sources. Compared to DLM, HMTBA has the same molar bioefficacy on improving the growth performance and carcass quality of broilers; however, HMTBA fed birds had superior meat color to DLM fed birds.
Broilers;Carcass Quality;Growth Performance;DL-methionine;2-Hydroxy-4-(Methylthio) Butanoic Acid;
 Cited by
Effects of DL-2-hydroxy-4-(methylthio) butanoic acid on broilers at different dietary inclusion rates, British Poultry Science, 2015, 56, 3, 337  crossref(new windwow)
The level and source of free-methionine affect body composition and breast muscle traits in growing broilers, Poultry Science, 2016, 95, 10, 2322  crossref(new windwow)
Impact of feeding 2-hydroxy-4-(methylthio)butanoic acid andDL-methionine supplemented maize–soybean–rapeseed meal diets on growth performance and carcase quality of broilers, British Poultry Science, 2007, 48, 2, 190  crossref(new windwow)
Effect of methionine supplementation in chicken feed on the quality and shelf life of fresh poultry meat, Poultry Science, 2017  crossref(new windwow)
Bioavailability of liquid methionine hydroxy analogue-free acid relative to DL-methionine in broilers, Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2013, 61, 5, 1513  crossref(new windwow)
Dietary methionine requirement of Jing Brown layer hens from 9 to 17 weeks of age, Journal of Animal Physiology and Animal Nutrition, 2016  crossref(new windwow)
Andersen, S. B. 1991. Determination of tryptophan with HPLC after alkaline hydrolysis in autoclave using $\alpha$-methyltryptophan as internal standard. Acta Agric. Scand. 41:305-309

AOAC. 1989. Official Methods and Recommended Pages 8-78 in: Practices of the American Oil Chemistry Society. 4th ed (Ed. D. Firestone). American Oil Chemistry Society, Champaign, IL

AOAC. 1990. Official Methods of Analysis (15th Ed.). Association of Official Analytical Chemists, Washington, DC

Daenner, E. and W. Bessei. 2003. Influnce of supplementation with liquid DL-methionine hydroxy analogu-free acid (Alimet) or DL-methionine on performance of broilers. J. Appl. Poult. Res. 12:101-105

Dibner, J. J. 1983. Utilization of supplemental methionine sources by primary cultures of chick hepatocytes. J. Nutr. 113:2116-2123

Dibner, J. J. 2003. Review of the metabolism of 2-hydroxy-4-(methylthio)butanoic acid. World. Poult. Sci. J. 59:99-110 crossref(new window)

Dibner, J. J., R. C. Durley, J. G. Kostelc and F. J. Ivey. 1990. 2-Hydroxy-4-(methylthio) butanoic acid is a naturally occurring methionine precursor in the chick. J. Nutr. 120:553-560

Dibner, J. J. and C. D. Knight. 1984. Conversion of 2-hydroxy-4-(methylthio)butanoic acid to L-methionine in the chick: A stereospecific pathway. J. Nutr. 114:1716-1723

Dibner, J. J., C. D. Knight, R. A. Swick and F. J. Ivey. 1988. Absorption of 14C-2-hydroxy-4-(methylthio)butanoic acid (Alimet) from the hindgut of the broiler chick. Poult. Sci. 67:1314-1321

Drew, M. D., A. G. Van Kessel and D. D. Maenz. 2003. Absorption of methionine and 2-hydroxy-4-methylthiobutanoic acid in conventional and grem-free chickens. Poult. Sci. 82:1149-1153

Esteve-Garcia, E. and L. Llaurado. 1997. Performance, breast meat yield and abdominal fat deposition of male broiler chickens fed diets supplemented with DL-methionine or DLmethionine hydroxy analog free acid. Br. Poult. Sci. 38:397-404 crossref(new window)

Fletcher, D. L. 1999. Broiler breast meat color variation, pH and texture. Poult. Sci. 78:1323-1327

Froning, G. W. 1995. Color of poultry meat. Poult. Avian Biol. Rev. 6:83-93

Gaines, A. M., G. F. Yi, B. W. Ratliff, P. Scichana, G. L. Allee, C. D. Knight and K. R. Perryman. 2005a. Comparison of the relative bioefficacy of Alimet feed supplement and DLmethionine in nursery pigs. J. Anim. Sci. 83(Suppl. 2):161. (Abstr.)

Gaines, A. M., G. F. Yi, B. W. Ratliff, P. Srichana, D. C. Kendall, G. L. Allee, C. D. Knight and K. R. Perryman. 2005b. Estimation of the ideal ratio of tru ideal digestibile sulfur amino acids:lysine in 8- to 26-kg nursery pigs. J. Anim. Sci. 83:2527-2534

Garlich, J. D. 1985. Response of broiler to DL-Met hydroxy analogu free acid, DL-Met, and L-Met. Poult. Sci. 64:1541-1584

Gonzalez-Esqurra, R., M. Vazquz-Anon, T. Hampton, T. W. York, S. D. Peak, C. W. Wulling and C. D. Knight. 2004. Comparison of statistical models to calculate the relative bioefficacy of 2-hydroxy-4(methylthio) butanoic acid (HMB) and DL-methionine (DLM) for turkeys. Poult. Sci. 83 (Suppl.1):32 (Abstr.)

Guo, Y. M., Y. P. Zhou and J. L. Zhou. 1998. Effect of lysine and Met level on growth performance and carcass quality of broilers. Pages 24-30 in Nutrition and Feed Research in Broilers (Ed. Y. M. Guo). China Agricultural University Press, Beijing, P. R. China

Han, Y. M., F. Castanon, C. M. Parsons and D. H. Baker. 1990. Absorption and bioavailability of DL-methionine hydroxy analog compared to DL-methionine. Poult. Sci. 69:281-287

Jansman, A. J. M. and J. de Jong. 1999. Biological efficacy of Alimet$\circledR$ in weaned piglets (live weight range of 9-21 kg). TNO Nutr. Food. Res. Inst. V99.020. TNO Nutrition and Food Research Institute, Utrechtseweg, The Netherlands

Johri, T. S., A. B. Mandal and A. V. Elangovan. 2004. Comparing bio-efficacy of liquid DL-methionine hydroxy analogu free acid with DL-methionine in broiler chickens. Asian-Aust. J. Anim. Sci. 17:102-108

Knight, C. D., C. A. Atwell, C. W. Wulling, F. J. Ivey, and J. J. Dibner. 1998. The relative effectiveness of 2-hydroxy-4-(methylthio) butanoic acid and DL-methionine in young swine. J. Anim. Sci. 76:781-787

Knight, C. D. and J. J. Dibner. 1984. Comparative absorption of 2-hydroxy-4-(methylthio)-butanoic acid and L-methionine in the broiler chick. J. Nutr. 114:2179-2186

Leeson, S. and A. K. Zubair. 1997. Nutrition of the broiler chicken around the period of compensatory growth. Poult. Sci. 76:992-999

Lemme, A., D. Hoehler, J. J. Brennan and P. F. Mannion. 2002. Relative effectiveness of methionine hydroxy analog compared to DL-methionine in broiler chickens. Poult. Sci. 81:838-845

Liu, S. J. and Y. M. Guo. 2001. Effects of lysine and Met levels on carcass quality of female broiler chicks. Chinese J. Anim. Sci. 37:5-8

Liu, Y. L. and D. Y. Feng. 1998. Feeding and nutritional methods to improve carcass quality in pigs. Foreign Anim. Husbandry-Pigs Poult. 6:19-23

Liu, Y. L., G. F. Yi, G. L. Song, Y. Q. Hou, J. W. Huang, M. Vazquz-Anon and C. D. Knight. 2006. Impact of feeding 2-hydroxy-4-(methylthio) butanoic acid and DL-methionine supplemented corn-soybean-rapeseed meal diets on growth performance and carcass quality of broilers (Manuscript in submission to British Poultry Science)

Liu, Z., A. Bateman, M. Bryant, A. Abebe and D. Roland. 2004. Estimation of bioavailability of DL-methionine hydroxy analogu relative to DL-methionine in layers with exponential and slope-ratio models. Poult. Sci. 83:1580-1586

Luo, L., D. L. Wang, W. H. Zhang and J. C. Huang. 1994. Effects of dietary lysine and methionine levels on growth performance in male and female broilers. Chinese J. Anim. Sci. 30:8-10

Meirelles, H. T., R. Albuqurqu, L. M. O. Borgatti, L. W. O. Souza, N. C. Meister and F. R. Lima. 2003. Performance of broilers fed with different levels of methionine hydroxy analogu and DL-methionine. Braz. J. Poult. Sci. 69-74

Motl, M. A., C. A. Fritts and P. W. Waldroup. 2005a. Influnce of dietary sodium level on utilization of methionine from DLmethionine and liquid methionine-hydroxy analog. J. Appl. Poult. Res. 14:147-155

Motl, M. A., C. A. Fritts and P. W. Waldroup. 2005b. Effects of intestinal modification by antibiotics and antibacterials on utilization of methionine sources by broiler chickens. J. Appl. Poult. Res. 14:167-173

NRC. 1994. Nutrient requirements of poultry. 9th rev. ed. National Academy Press, Washington, DC

Ontiveros, R. R., W. D. Shermer and R. A. Berner. 1987. A HPLC method for 2-hydroxy-4-(methylthio)butanoic acid analysis. J. Agric. Food. Chem. 35:692-694 crossref(new window)

Richards, J. D., C. A. Atwell, M. Vazquz-Anon and J. J. Dibner. 2005. Comparative in vitro and in vivo absorption of 2-hydroxy-4-(methylthio) butanoic acid and methionine in the broiler chicken. Poult. Sci. 84:1397-1405

Romer, A. and H. J. Abel. 1999. Effects of DL-methionine hydroxyanalogu (MHA) or DL-methionine (DL-Met) on Nretention in broiler chickens and pigs. Anim. Feed Sci. Technol. 81:193-203 crossref(new window)

SAS Institute. 2003. $SAS^\circledR$ User's Guide: Statistics. Version 9.0. SAS Institute, Inc., Cary, NC

Schutte, J. B. and M. Pack. 1995. Sulfur amino acid requirement of broiler chick from fourteen to thirty eight days of age. 1. Performance and carcass yield. Poult. Sci. 74:480-487

Schwarz, G. 1978. Estimating the dimension of a model. Ann. Stat. 6:461-464 crossref(new window)

Van Weerden, E. J., J. B. Schutte and H. L. Bertram. 1992. Utilization of the polymers of Met hydroxy analog free acid (MHA-FA-FA) in broiler chicks. Arch. Geflugelkd. 56:63-68

Wallis, I. R. 1999. Dietary supplements of methionine increase breast meat yield and decrease abdominal fat in growing broiler chickens. Aust. J. Exp. Agric. 39:131-141 crossref(new window)

Yang, F. 1993. Animal Nutrition. Agriculture Press, Beijing, China

Yang, L. 1994. Modern Broiler Production. Beijing Agricultural University Press, Beijing, China

Yu, J. S. and B. F. Zeng. 1996. The factors to influnce broiler meat color and the methods to improve broiler meat color. Guangdong Feed 6:10-12

Zhang, Y. C. 2001. Ileal amino acids digestibility of Chinese cottonseed meals from different cultivars and different processing conditions in pigs. Ph.D. Dissertation, China Agricultural University, Beijing, P. R. China

Zimmermann, B., R. Mosenthin, M. Rademacher, P. B. Lynch and E. Esteve-Garcia. 2005. Comparative studies on the relative efficacy of DL-methionine and liquid methionine hydroxy analogu in growing pigs. Asian-Aust. J. Anim. Sci. 18:1003-1010