JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Candidate Genes with Ovulation by Differential Display PCR in Small Tail Han Sheep
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Candidate Genes with Ovulation by Differential Display PCR in Small Tail Han Sheep
Liu, Shufang; Li, Hongbin; Song, Xuemei; Wang, Aihua; Wei, Caihong; Du, Lixin;
  PDF(new window)
 Abstract
To find the candidate genes concerned with ovulation rate of sheep, Differential Display Reverse Transcription Polymerase Chain Reaction was employed to find the differently expressed cDNA controlling ovulation in the Small Tail Han sheep of polyembryony and in Tan sheep of single birth. Twenty-four primer pairs of three anchored primers and eight arbitrary primers were assembled to amplify the specialized bands from these sheep. Positive cross tests were applied to optimize the ascertainable PCR conditions in which different special bands can be identified by silver strain in one PCR tube. After eliminating the false positive PCR products by Northern hybridization, 24 differential display bands were acquired from the ovary in the Small Tail Han sheep. These EST bands were sequenced and 18 different ESTs were found in which five ESTs had several copies and 13 ESTs had only one copy. Comparing these ESTs with homologous sequences by BLAST in the GenBank, there were six ESTs with known open reading frame (ORF) and function, three ESTs with known ORF and no function, and 9 ESTs without homologous sequence. These ESTs partly represent several genes such as NOS2, tensin, TCRA, CDKN1A, ESR1 and ACTB which express especially in Small Tail Han sheep.
 Keywords
Ovary;Little Size;Small Tail Han Sheep;DD-PCR;
 Language
English
 Cited by
 References
1.
Bayir, H. H., V. E. Kagan, G. G. Borisenko, Y. Y. TYurina, K. Janesko, V. A. Vagni, T. R. Billiar, D. L. Williams and P. M. Kochanek. 2005. Enhanced oxidative stress in iNOS-deficient mice after traumatic brain injury: support for a neuroprotective role of iNOS. J. Cereb Blood Flow Metab. 25:673-684 crossref(new window)

2.
Bhatia, S. and R. Arora 2005. Biodiversity and Conservation of Indian sheep genetic resources. - An Overview - Asian-Aust. J. Anim. Sci. 18(10):1387-1402

3.
Chu, M. X., J. Z. Wang, A. G. Wang, N. Li and J. L. Fu. 2003. Association analysis between five microsatellite loci and litter size in small Tail Han sheep. Asian-Aust. J. Anim. Sci. 16:1555-1559

4.
Dahlen, A., C. D. Fletcher, F. Mertens, J. A. Fletcher, A. R. Perez- Atayde, M. J. Hicks, Debiec-RYchterM, R. Sciot, Wejde, R. Welin, Mandahln, Panagopoulos l. 2004. Activation of the GLI oncogene through fusion with the beta-actin gene (ACTB) in a group of distinctive pericytic neoplasms: pericytoma with t(7;12). Am. J. Pathol. May 164:1645-1653

5.
Doss, R. P. 1996.Differential display without radioactivity a modified procedure. Bio. Techniq. 21: 410-412

6.
Du, Lixin and Cao Dingguo. 2003. Genetic Analysis on Littletailed Han Sheep by Microsatellite and RAPD Markers, ACTA Genetica Sinica. 30:1041-1044

7.
Guo, W. M., X. Chu, X. M. Deng, J. D. Feng, Ning Li and Changxin Wu. 2004. Association of a single codon deletion in bone morphogenetic protein 15 gene with prolificacy in small tail Han sheep. Asian-Aust. J. Anim. Sci. 17(11):1491-1501

8.
Jia, Cunling, Ning Li, Xingbo Zhao, Xiaoping Zhu and Zhihai Jia. 2005. Association of single nucleotide polymorphisms in exon 6 region of BMPRIB gene with litter size traits in sheep. Asian-Aust. J. Anim. Sci. 18(10):1375-1378

9.
Kato, R. and H. Ogawa. 1994. An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae. Nucleic Acids Res. 22:3104-3112 crossref(new window)

10.
Ramiro, A. R., M. N. Navarro, A. Carreira, Y. R. Carrasco, V. G. de Yebenes, G. Carrillo, J. L. San Millan, B. Rubin and M. L. Toribio. 2001 .Differential developmental regulation and functional effects on pre-TCR surface expression of human pTalpha(a) and pTalpha(b) spliced isoforms. J. Immunol. 167:5106-5114

11.
Rishi, A. K., C. K. Hsu, X. S. Li, A. Hussain, T. M. Gerald and J. A. Fontana. 1997. Transcriptional repression of the cyclindependent kinase inhibitor p21WAF1/CIP1 gene mediated by cis elements present in the 3'-untranslated region. Cancer Res. 57:5129-5136

12.
Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, Second Edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY)

13.
Sanchez, T., S. Thangada, M. T. Wu, C. D. Kontos, D. Wu, H. Wu and T. Hla. 2005. PTEN as an effector in the signaling of antimigratory G protein-coupled receptor. Proc. Natl. Acad. Sci. USA. 102:4312-4317

14.
Sharma, R. C., A. L. Arora, A. K. Mishra, S. Kumar and V. K. Singh. 2004. Breeding prolific garole with malpura sheep for increased reproductive efficiency in semi arid tropics of India. Asian-Aust. J. Anim. Sci. 17(6):737-742

15.
Weaver, K. R., G. Caetano-Anolles, P. M. Gresshoff and L. M. Callahan. 1994. Isolation and cloning of DNA amplification products from silver stained polyacrylamide gels. Bio. Techniq. 16: 226-230