Advanced SearchSearch Tips
Antioxidative and Probiotic Properties of Lactobacillus gasseri NLRI-312 Isolated from Korean Infant Feces
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Antioxidative and Probiotic Properties of Lactobacillus gasseri NLRI-312 Isolated from Korean Infant Feces
Kim, H.S.; Jeong, S.G.; Ham, J.S.; Chae, H.S.; Lee, J.M.; Ahn, C.N.;
  PDF(new window)
We selected a Lactobacillus spp. from Korean healthy infant feces based upon their antioxidant activity. This strain was identified as Lactobacillus gasseri by 16S rDNA sequencing, and named Lactobacillus gasseri NLRI-312. In the present study, we investigate the protective effect of this strain on the induced damage to cellular membrane lipid and DNA in Jurkat cells. To estimate the extent of cellular lipid peroxidation inhibition, MDA (malondialdehyde) was measured, and DNA damage was tested by the comet assay. We also examined probiotic properties including tolerance to acid and bile, antibiotic resistance. From the results obtained, the supplementation of Jurkat cells with NLRI-312 decreased in DNA damage, while no effect was shown on MDA decrease. In probiotic properties, this strain was resistance to both acid and bile, showed considerably higher survival when incubated in pH 2 or 1% bile salts (w/v). We concluded that the NLRI-312 could be used as potential probiotic bacteria, with the effect of reducing DNA damage induced by .
Lactobacillus gasseri;Antioxidant;Probiotic;DNA Damage;Jurkat Cell;
 Cited by
Annuk, H., J. Shchepetova, T. Kullisaar, E. Songisepp, M. Zilmer.and M. Mikelsaar. 2003. Characterization of intestinal lactobacilli as putative probiotic candidates. J. Appl. Microbiol. 94:403-412 crossref(new window)

Bei, W. J., W. Peng, Y. Ma and A. Xu. 2004. NaoXinQing, an antistroke herbal medicine, reduces hydrogen peroxide induced injury in NG 108-15 cells. Neurosci. Lett. 363:262-265 crossref(new window)

Charteris, W. P., P. M. Kelly, L. Morelli and J. K. Collins. 1998. Ingredient selection criteria for probiotic microorganisms in functional dairy foods. Intl. J. Dairy Technol. 51:123-136 crossref(new window)

Clark, P. A., L. N. Cotton and J. H. Martin. 1993. Selection of bifidobacteria for use as dietary adjuncts in cultured dairy foods: II-Tolerance to stimulated pH of human stomachs. Cult. Dairy Prod. J. 28:11-14

Collins, J. K., G. Thornton and G. O. Sullivan. 1998. Selection of probiotic strains for human application. Intl. Dairy J. 8:487-490 crossref(new window)

Conway, P. L., S. L. Gorbach and B. R. Goldin. 1987. Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. J. Dairy Sci. 70:1-12

Dunne, C., L. O'Mahony, L. Murphy, G. Thornton, D. Morrissey, S. O'Halloran, M. Feeney, S. Flynn, G. Fitzgerald, C. Daly, B. Kiely, G. O'Sullivan, F. Shanahan and J. K. Collins. 2001. In vitro selection criteria for probiotic bacteria of human origin: Correlation with in vivo findings. Am. J. Clin, Nutr. 73:386S-392S

Duthie, S. J., M. A. Ross and A. R. Collins. 1996. Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes. Cancer Res. 56:1291-1295

Erba, D., P. Riso, A. Colombo and G. Testilin. 1999. Supplementation of Jurkat T cells with green tea extract decreases oxidative damage due to iron treatment. J. Nutr. 129:2130-2134

Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66:365-378

Gilliland, S. E., T. E. Stanley and L. J. Bush. 1984. Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J. Dairy Sci. 67:3045-3051

Gilliland, S. E. and D. K. Walker. 1990. Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjuncts to produce a hypocholesterolemic effect in humans. J. Dairy Sci. 73:905-911

Halliwell, B. and J. M. C. Gutteridge. 1990. The antioxidants of human extracellular fluids. Arch. Biochem. Biophys. 280:1-8 crossref(new window)

Havenaar, R., B. Ten Brink and J. H. J. Huis In'tVwld. 1992. Selection of strains for probiotic use. In: (Ed. R. Fuller), Probiotics-the Scientific Basis. Chapman & Hall, London, pp. 209-221

Hyronimus, B., C. Le Marrec, A. Hadj Sassi and A. Deschamps. 2000. Acid and bile tolerance of spore-forming lactic acid bacteria. Intl. J. Food Microbiol. 61:193-197 crossref(new window)

Jacobesen, C. N., V. Posenfeldt Nielsen, A. E. Hayford, P. L. Moller, K. F. Michaelsen, A. Pærregaard, B. Sandström, M. Tvede and M. Jakobsen.1999. Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in-vitro techniques and evaluation of the colonization ability of five selected strains in humans. Appl. Environ. Microbiol. 65:4949-4956

Kim, H. S., H. S. Chae, S. G. Jeong, J. S. Ham, S. K. Im, C. N. Ahn and J. M. Lee. 2005. Antioxidant activity of some yogurt starter cultures. Asian-Aust. J. Anim. Sci. 18:255-258

Kim, H. S., H. S. Chae, S. G. Jeong, J. S. Ham, S. K. Im, C. N. Ahn and J. M. Lee. 2006. In vitro Antioxidative properties of Lactobacilli. Asian-Aust. J. Anim. Sci. 19:262-265

Kimoto, H., S. Ohmomo, M. Nomura, M. Kobayashi and T. Okamoto. 2000. In vitro studies on probiotic properties of lactococci. Milchwissenschaft. 55:245-249

Kirjavainen, P. V., A. C. Ouwehand, E. Isolauri and S. J. Salminen. 1998. The ability of probiotic bacteria to bind to human intestinal mucus. FEMS Microbiol. Lett. 167:185-189 crossref(new window)

Kullisaar, T., M. Zilmer, M. Mikelsaar, T. Vihalemm, H. Annuk, C. Kairane and A. Kilk. 2002. Two antioxidative lactobacilli strains as promising probiotics. Intl. Food Microbiol. 72:215-224 crossref(new window)

Lankaputhra, W. E. V. and N. P. Shah. 1995. Survival of Lactobacillus acidophilus and Bifidobacterium spp. in the presence of acid and bile salts. Cult. Dairy Prod. J. 30:2-7

Lee, Y. K. and S. Salminen. 1995. The coming of age of probiotics. Trends in Food Sci. Tech. 6:241-245 crossref(new window)

Lin, M. Y. and F. J. Chang. 2000. Antioxidative effect of intestinal bacteria Bifidobacterium longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356. Digestive Diease Sci. 45:1617-1622 crossref(new window)

Pennacchia, D., D. Ercolini, G. Blaiotta, O. Pepe, G. Mauriello and F. Villani. 2004. Selection of Lactobacillus strains from fermented sausages for their potential use as probiotics. Meat Sci. 67:309-317 crossref(new window)

Riso, P., A. Santangelo and M. Porrini. 1999. The comet assay for the evaluation of cell resistance to oxidative stress. Nutr. Res. 19:325-333 crossref(new window)

Riso, P., D. Erba, F. Criscuoli and G. Testolin. 2002. Effect of green tea extract on DNA repair and oxidative damage due to $H_2O_2$ in Jurkat cells. Nutr. Res. 22:1143-1150 crossref(new window)

Saavedra, J. M. 2001. Clinical application of probiotic agents. Am. J. Clin. Nutr. 73:1147S-1151S

Singh, N. P., M. T. McCoy, R. R. Tice and E. L. Schneider. 1998. A simple technique for quantitation of low levels of DNA damage in individuals cells. Exp. Cell. Res. 175:184-191

Tompson, J. D., D. G. Higgins and T. J. Gibson. 1994. CLUSTAL W; improving the sensitivity of progressive multiple sequences alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680 crossref(new window)

Tanaka, K. I., N. Fujita and M. Yoshioka. 2001. Immunosuppressive and non-immunosuppressive immunophilin improve $H_2O_2$-induced cell damage by increasing glutathione levels in NG108-15 cells. Brain Res. 889:225-228 crossref(new window)

Weisburg, W. A., S. M. Barns, D. A. Pelletier and D. J. Lane. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173:697-703

Xanthopoulous, V., E. Litoloulou-Tzanetaki and N. Tzanetakis. 2000. Characterization of Lactobacillus isolates from infant feces as dietary adjuncts. Food Microbiol. 17:205-215 crossref(new window)

Xiao, Q. X., N. T. K. Lee, P. R. Carlier, Y. P. Pang and Y. F. Han. 2000. Bis (7)-tacrine, a promising anti-Alzheimer's agent, reduces hydrogen peroxide-induced injury in rat pheochromocytoma cells: comparison with tacrine. Neurosci. Lett. 290:197-200 crossref(new window)

Zavaglia, A. G., G. Kociubinsky, P. Pérez and G. de Antoni. 1998. Isolation and characterization of Bifidobacterium strains for probiotic formulation. J. Food Protec. 61:865-873