Advanced SearchSearch Tips
ASCL2 Gene Expression Analysis and Its Association with Carcass Traits in Pigs
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
ASCL2 Gene Expression Analysis and Its Association with Carcass Traits in Pigs
Cheng, H.C.; Zhang, F.W.; Deng, C.Y.; Jiang, C.D.; Xiong, Y.Z.; Li, F.E.; Lei, M.G.;
  PDF(new window)
Achaete-scute like 2 (ASCL2) gene encodes a member of the basic helix-loop-helix transcription factor which is essential for the maintenance of proliferating trophoblasts during placental development. ASCL2 gene preferentially expresses the maternal allele in the mouse. However, it escapes genomic imprinting in the human. In this study, the complete open reading frame consisting of 193 amino acids of ASCL2 gene was obtained. Sequence analysis indicated that a C-G mutation existed in the 3' region between Meishan and Large White pigs. The polymorphism was used to determine the monoallelic or biallelic expression with RT-PCR-RFLP in pigs of Large hybrids. Imprinting analysis indicated that the ASCL2 gene expression was biallelic in all the tested tissues (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, ovary and pituitary). PCR-RFLP was used to detect the polymorphism in 270 pigs of the "" resource population. The statistical results showed highly significant associations of the genotypes and fat meat percentage (FMP), lean meat percentage (LMP) and ratio of lean to fat (RLF) (p<0.01), and significant associations of the genotypes and loin eye area (LEA) and internal fat rate (IFR) (p<0.05).
ASCL2;Imprinting;Porcine;Carcass Traits;Polymorphism;
 Cited by
Comparative Analysis of Repetitive Elements of Imprinting Genes Reveals Eleven Candidate Imprinting Genes in Cattle,;;

아세아태평양축산학회지, 2009. vol.22. 6, pp.893-899 crossref(new window)
Imprinted Gene mRNA Expression during Porcine Peri-implantation Development,;;;;;;;;;;;

아세아태평양축산학회지, 2010. vol.23. 6, pp.693-699 crossref(new window)
Assessment of genomic imprinting of PPP1R9A, NAP1L5 and PEG3 in pigs, Russian Journal of Genetics, 2011, 47, 4, 471  crossref(new windwow)
Alexander, L. J., D. L. Troyer, G. A. Rohrer, T. P. Smith, L. B. Schook and C. W. Beattie. 1996. Physical assignments of 68 porcine cosmid and lambda clones containing polymorphic microsatellites. Mamm. Genome. 7:368-372. crossref(new window)

Andersson, L., C. S. Haley, H. Ellegren, S. A. Knott, M. Johansson, K. Andersson, L. Andersson-Eklund, I. Edfors-Lilja, M. Fredholm and I. Hansson. 1994. Genetic mapping of quantitative trait loci for growth and fatness in pigs. Sci. 263:1771-1774. crossref(new window)

Dai, L. H., Y. Z. Xiong, C. Y. Deng, S. W. Jiang, B. Zuo, R. Zheng, F. E. Li and M. G. Lei. 2006. Association of the A-G polymorphism in porcine adiponectin gene with fat deposition and carcass traits. Asian-Aust. J. Anim. Sci. 19:779-783. crossref(new window)

Estelle, J., A. Mercade, J. L. Noguera, M. Perez-Enciso, C. Ovilo, A. Sanchez and J. M. Folch. 2005. Effect of the porcine IGF2- intron3-G3072A substitution in an outbred Large White population and in an Iberian${\times}$Landrace cross. J. Anim. Sci. 83:2723-2728.

Guillemot, F., T. Caspary, S. M. Tilghman, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, D. J. Anderson, A. L. Joyner, J. Rossant and A. Nagy. 1995. Genomic imprinting of Mash2, a mouse gene required for trophoblast development. Nat. Genet. 9:235-242. crossref(new window)

Jeon, J. T., O. Carlborg, A. Tornsten, E. Giuffra, V. Amarger, P. Chardon, L. Andersson-Eklund, K. Andersson, I. Hansson, K. Lundstrom and L. Andersson. 1999. A paternally expressed QTL affecting skeletal and cardiac muscle mass in pigs maps to the IGF2 locus. Nat. Genet. 21:157-158. crossref(new window)

Jubb, A. M., S. Chalasani, G. D. Frantz, R. Smits, H. I. Grabsch, V. Kavi, N. J. Maughan, K. J. Hillan, P. Quirke and H. Koeppen. 2006. Achaete-scute like 2 (ascl2) is a target of Wnt signalling and is upregulated in intestinal neoplasia. Oncogene. 25:3445-3457. crossref(new window)

Jungerius, B. J., A. S. van Laere, M. F. Te Pas, B. A van Oost, L. Andersson and M. A. Groenen. 2004. The IGF2-intron3- G3072A substitution explains a major imprinted QTL effect on backfat thickness in a Meishan${\times}$European white pig intercross. Genet. Res. 84:95-101. crossref(new window)

Koning, D. J., A. P. Rattink, B. Harlizius, J. A. Arendonk, E. W. Brascamp and M. A. Groenen. 2000. Genome-wide scan for body composition in pigs reveals important role of imprinting. Proc. Natl. Acad. Sci. USA. 97:7947-7950 crossref(new window)

Lee, M., P. S. Brandenburg, G. M. Landes, M. Adams, G. Miller and A. P. Feinberg. 1999. Two novel genes in the center of the 11p15 imprinted domain escape genomic imprinting. Hum. Mol. Genet. 8:683-690. crossref(new window)

Liu, B. H. 1998. Statistical genomics: linkage, mapping, and QTL analysis. CRC Press, LLC

Miyamoto, T., S. Hasuike, Y. Jinno, H. Soejima, K. Yun, K. Miura, M. Ishikawa and N. Niikawa. 2002. The human ASCL2 gene escaping genomic imprinting and its expression pattern. Assist. Reprod. Genet. 19:240-244. crossref(new window)

Nezer, C., L. Moreau, B. Brouwers, W. Coppieters, J. Detilleux, R. Hanset, L. Karim, A. Kvasz, P. Leroy and M. Georges. 1999. An imprinted QTL with major effect on muscle mass and fat deposition maps to the IGF2 locus in pigs. Nat. Genet. 21:155-156. crossref(new window)

Nolan, C. M., J. K. Killian, J. N. Petitte and R. L. Jirtle. 2001. Imprint status of M6P/IGF2R and IGF2 in chickens. Dev. Genes. Evol. 211:179-183. crossref(new window)

Reik, W., F. Santos and W. Dean. 2003. Mammalian epigenomics: reprogramming the genome for development and therapy. Theriogenol. 59:21-32. crossref(new window)

Smith, R. J., W. Dean, G. Konfortova and G. Kelsey. 2003. Identification of novel imprinted genes in a genome-wide screen for maternal methylation. Genome Res. 13:558-569. crossref(new window)

Stepan, H., W. Marqwardt, Y. Kuhn, M. Hockel, H. P. Schultheiss and T. Walther. 2003. Structure and regulation of the murine Mash2 gene. Biol. Reprod. J. 68:40-44. crossref(new window)

Wang, G., B. Yan, X. Deng, C. Li, X. Hu and N. Li. 2005. Insulinlike growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken. Sci. China. C. Life. Sci. 48:187-194. crossref(new window)

Xiong, Y. Z. and C. Y. Deng. 1999. Technical testing rule of lean pig. Chinese Agricultural Press, Beijing.

Zhang, F. W., H. C. Cheng, C. Y. Deng, Y. Z. Xiong, F. E. Li and M. G. Lei. 2006. cDNA cloning, tissue expression and association of porcine pleiomorphic adenoma gene-like 1 (PLAGL1) gene with carcass traits. Asian-Aust. J. Anim. Sci. 19:1257-1261. crossref(new window)