Advanced SearchSearch Tips
Molecular Cloning and Characterization of Bovine HMGA1 Gene
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Molecular Cloning and Characterization of Bovine HMGA1 Gene
Yu, S.L.; Chung, H.J.; Sang, B.C.; Bhuiyan, M.S.A.; Yoon, D.; Kim, K.S.; Jeon, J.T.; Lee, J.H.;
  PDF(new window)
The high mobility group AT-hook1 (HMGA1) proteins are known to be related to the regulation of gene transcription, replication and promotion of metastatic progression in cancer cells. The loss of expression by disrupting the HMGA1 gene affects insulin signaling and causes diabetes in the mouse. Previously identified single nucleotide polymorphism (SNP) of HMGA1 was significantly associated with fat deposition traits in the pig. In this study, we identified 3,935 bp nucleotide sequences from exon 5 to exon 8 of the bovine HMGA1 gene and its mRNA expression was observed by quantitative real-time PCR. Six single nucleotide polymorphisms in the bovine HMGA1 gene were detected and the allele frequencies of these SNPs were investigated using the PCR-RFLP method in nine cattle breeds including Limousin, Simmental, Brown Swiss, Hereford, Angus, Charolais, Hanwoo, Brahman and Red Chittagong cattle. The map location showed that the bovine HMGA1 gene was also closely located with a previously identified meat quality QTL region indicating this gene is the most likely positional candidate for meat quality traits in cattle.
HMGA1;SNP;Meat Quality Traits;Cattle;
 Cited by
Aldrich, T. L., A. E. Morris and J. N. Thomas. 1999. HMG-I(Y) proteins implicated in amplification of CHO cell DNA. Sequence at NCBI AF193762 and AF193763. Unpublished manuscript.

Altschul, S. F., W. Gish, W. Miller, E. W. Myers and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215(3):403-410.

Banks, G. C., B. Mohr and R. Reeves. 1999. The HMG-I(Y) A.Thook peptide motif confers DNA-binding specificity to a structured chimeric protein. J. Biol. Chem. 274(23):16536-16544. crossref(new window)

Bidanel, J. P. and M. Rothschild. 2002. Current status of quantitative trait locus mapping in pigs. Pig News and Information 23(2):39N-53N.

Bussemakers, M. J., W. J. van de Ven, F. M. Debruyne and J. A. Schalken. 1991. Identification of high mobility group protein I(Y) as potential progression marker for prostate cancer by differential hybridization analysis. Cancer Res. 51(2):606-611.

Bustin, M. 1999. Regulation of DNA-dependent activities by the functional motifs of the high-mobility-group chromosomal proteins. Mol. Cell. Biol. 19(8):5237-5246.

Casas, E., S. D. Shackelford, J. W. Keele, M. Koohmaraie, T. P. Smith and R. T. Stone. 2003. Detection of quantitative trait loci for growth and carcass composition in cattle. J. Anim. Sci. 81:2976-2983.

Cheong, H. S., D. H. Yoon, A. H. Kim, B. L. Park, E. R. Chung, H. J. Lee, I. C. Cheong, S. J. Oh and H. D. Shin. 2006. Leptin polymorphisms associated with carcass traits of meat in Korea cattle. Asian-Aust. J. Anim. Sci. 19(11):1529-1535.

Chiappetta, G., V. Avantaggiato, R. Visconti, M. Fedele, S. Battista, F. Trapasso, B. M. Merciai, V. Fidanza, V. Giancotti, M. Santoro, A. Simeone and A. Fusco. 1996. High level expression of the HMGI (Y) gene during embryonic development. Oncogene. 13(11):2439-2446.

Chung, E. R. and W. T. Kim. 2005. Associated of SNP marker in IGF-1 and MYF5 candidate genes with growth traits in Korean cattle. Asian-Aust. J. Anim. Sci. 18(8):1061-1065.

Everts-van der Wind, A., S. R. Kata, M. R. Band, M. Rebeiz, D. M. Larkin, R. E. Everts, C. A. Green, L. Liu, S. Natarajan, T. Goldammer, J. H. Lee, S. McKay, J. E. Womack and H. A. Lewin. 2004. A 1463 gene cattle-human comparative map with anchor points defined by human genome sequence coordinates. Genome Res. 14(7):1424-1437. crossref(new window)

Foti, D., E. Chiefari, M. Fedele, R. Iuliano, L. Brunetti, F. Paonessa, G. Manfioletti, F. Barbetti, A. Brunetti, C. M. Croce, A. Fusco and A. Brunetti. 2005. Lack of the architectural factor HMGA1 causes insulin resistance and diabetes in humans and mice. Nat. Med. 11:765-773. crossref(new window)

Johnson, K. R., D. A. Lehn and R. Reeves. 1989. Alternative processing of mRNAs encoding mammalian chromosomal high-mobility-group proteins HMG-I and HMG-Y. Mol. Cell. Biol. 9(5):2114-2123.

Johnson, K. R., D. A. Lehn, T. S. Elton, P. J. Barr and R. Reeves. 1988. Complete murine cDNA sequence, genomic structure, and tissue expression of the high mobility group protein HMGI(Y). J. Biol. Chem. 263(34):18338-18342.

Kim, J. J., F. Farnir, J. Savell and J. F. Taylor. 2003. Detection of quantitative trait loci for growth and beef carcass fatness traits in a cross between Bos taurus (Angus) and Bos indicus (Brahman) cattle. J. Anim. Sci. 81:1933-1942.

Kim, K. S., H. Thomsen, J. Bastiaansen, N. T. Nguyen, J. C. M. Dekkers, G. S. Plastow and M. F. Rothschild. 2004. Investigation of obesity candidate genes on porcine fat deposition quantitative trait loci regions. Obes. Res. 12:1981-1994. crossref(new window)

Kim, K. S., J. J. Lee, H. Y. Shin, B. H. Choi, C. K. Lee, J. J. Kim, B. W. Cho and T. H. Kim. 2006. Association of melanocortin 4 receptor (MC4R) and high mobility group AT-hook1 (HMGA1) polymorphisms with pig growth and fat deposition traits. Anim. Genetics. 37:419-421. crossref(new window)

Kong, H. S., J. D. Oh, J. H. Lee, D. H. Yoon, Y. H. Choi, B. W. Cho, H. K. Lee and G. J. Jeon. 2007. Association of sequence variations in DGAT1 gene with economic traits in Hanwoo (Korean cattle). Asian-Aust. J. Anim. Sci. 20(6):817-820.

Kong, H. S., J. D. Oh, S. G. Lee, Y. S. Hong, W. I. Song, S. J. Lee, H. C. Kim, B. H. Yoo, H. K. Lee and G. J. Jeon. 2006. Association of polymorphisms in the bovine Leptin gene with ultrasound measurements for improving in Korean cattle. Asian-Aust. J. Anim. Sci. 19(12):1691-1695.

Kumar, S., K. Tamura and M. Nei. 2004. MEGA3.1: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief. Bioinform. 5(2):150-163. crossref(new window)

Melillo, R. M., G. M. Pierantoni, S. Scala, S. Battista, M. Fedele, A. Stella, M. C. De Biasio, G. Chiappetta, V. Fidanza, G. Condorelli, M. Santoro, C. M. Croce, G. Viglietto and A. Fusco. 2001. Critical role of the HMGI(Y) proteins in adipocytic cell growth and differentiation. Mol. Cell. Biol. 21(7):2485-2495. crossref(new window)

Moore, S., L. Alexander, M. Brownstein, L. Guan, S. Lobo, Y. Meng, M. Tanaguchi, Z. Wang, J. Yu, C. Prange, K. Schreiber, C. Shenmen, L. Wagner, M. Bala, S. Barbazuk, S. Barber, R. Babakaiff, J. Beland, E. Chun, L. Del Rio, S. Gibson, R. Hanson, R. Kirkpatrick, J. Liu, C. Matsuo, M. Mayo, R. R. Santos, J. Stott, M. Tsai, D. Wong, A. Siddiqui, R. Holt, S. J. Jones and M. A. Marra. 2006. Sequence at NCBI BC120383. Unpublished manuscript.

Murua Escobar, H., J. T. Soller, A. Richter, B. Meyer, S. Winkler, J. Bullerdiek and I. Nolte. 2005. 'Best Friends' sharing the HMGA1 gene: comparison of the human and canine hmga1 to orthologous other species. J. Hered. 96:777-781. crossref(new window)

Murua Escobar, H., J. T. Soller, A. Richter, B. Meyer, S. Winkler, A. M. Flohr, I. Nolte and J. Bullerdiek. 2004. The canine HMGA1. Gene. 330:93-99. crossref(new window)

Reeves, R. 2001. Molecular biology of HMGA proteins: hubs of nuclear function. Gene. 277:63-81. crossref(new window)

Reeves, R. and L. Beckerbauer. 2001. HMGI/Y proteins: flexible regulators of transcription and chromatin structure. Biochim. Biophys. Acta. 1519(1-2):13-29.

Sgarra, R., F. Diana, C. Bellarosa, A. Rustighi, M. Toller, G. Manfioletti and V. Giancotti. 2002. Increase of HMGA1a protein methylation is a distinctive characteristic of tumor cells induced to apoptosis. Sequence at NCBI AF511040, Unpublished manuscript.

Shin, S. C. and E. R. Chung. 2007. Association of SNP marker in the Leptin gene with carcass and meat quality traits in Korean cattle. Asian-Aust. J. Anim. Sci. 20(1):1-6.

Shin, S. C. and E. R. Chung. 2007. Association of SNP marker in the Thyroglobulin gene with carcass and meat quality traits in Korean cattle. Asian-Aust. J. Anim. Sci. 20(2):172-177.

Shin, S. C. and E. R. Chung. 2007. SNP Detection of Carboxypeptidase E gene and its association with meat quality and carcass traits in Korean cattle. Asian-Aust. J. Anim. Sci. 20(3):328-333.

Shin, S. C., M. J. Kang and E. R. Chung. 2007. Identification of a novel SNP associated with meat quality in C/EBP ${\beta}$ gene of Korean cattle. Asian-Aust. J. Anim. Sci. 20(4):466-470.

Slonim, D., L. Kruglyak, L. Stein and E. Lander. 1997. Building human genome maps with radiation hybrids. J. Comput. Biol. 4:487-504. crossref(new window)

Strausberg, R. L., E. A. Feingold, L. H. Grouse, J. G. Derge, R. D. Klausner, F. S. Collins, L. Wagner, C. M. Shenmen, G. D. Schuler, S. F. Altschul, B. Zeeberg, K. H. Buetow, C. F. Schaefer, N. K. Bhat, R. F. Hopkins, H. Jordan, T. Moore, S. I. Max, J. Wang, F. Hsieh, L. Diatchenko, K. Marusina, A. A. Farmer, G. M. Rubin, L. Hong, M. Stapleton, M. B. Soares, M. F. Bonaldo, T. L. Casavant, T. E. Scheetz, M. J. Brownstein, T. B. Usdin, S. Toshiyuki, P. Carninci, C. Prange, S. S. Raha, N. A. Loquellano, G. J. Peters, R. D. Abramson, S. J. Mullahy, S. A. Bosak, P. J. McEwan, K. J. McKernan, J. A. Malek, P. H. Gunaratne, S. Richards, K. C. Worley, S. Hale, A. M. Garcia, L. J. Gay, S. W. Hulyk, D. K. Villalon, D. M. Muzny, E. J. Sodergren, X. Lu, R. A. Gibbs, J. Fahey, E. Helton, M. Ketteman, A. Madan, S. Rodrigues, A. Sanchez, M. Whiting, A. Madan, A. C. Young, Y. Shevchenko, G.. G. Bouffard, R. W. Blakesley, J. W. Touchman, E. D. Green, M. C. Dickson, A. C. Rodriguez, J. Grimwood, J. Schmutz, R. M. Myers, Y. S. Butterfield, M. I. Krzywinski, U. Skalska, D. E. Smailus, A. Schnerch, J. E. Schein, S. J. Jones and M. A. Marra. 2002. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA. 99(26):16899-16903.

Thompson, J. D., D. G. Higgins and T. J. Gibson. 1994: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680. crossref(new window)

Vandenplas, M., M. M. Cordonnier-Pratt, Y. Suzuki, S. Sugano, J. N. Moore, C. Liang, F. Sun, R. Sullivan, M. Shah and L. H. Pratt. 2003. An EST database from equine (Equus caballus) unstimulated peripheral blood leukocytes. Sequence at NCBI CD535395. Unpublished manuscript.

Womack, J. E., J. S. Johnson, E. K. Owens, C. E. 3rd. Rexroad, J. Schlapfer and Y. P. Yang. 1997. A whole-genome radiation hybrid panel for bovine gene mapping. Mamm. Genome. 8:854-864. crossref(new window)

Zuo, B., Y. Z. Xiong, Y. H. Su, C. Y. Deng, M. G. Lei, R. Zheng, S. W. Jiang and F. E. Li. 2004. Mapping of quantitative trait loci on porcine chromosome 7 using combined data analysis. Asian-Aust. J. Anim. Sci. 17(10):1350-1354.