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Individual Identification and Breed Allocation with Microsatellite Markers: An Evaluation in Indian Horses
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 Title & Authors
Individual Identification and Breed Allocation with Microsatellite Markers: An Evaluation in Indian Horses
Behl, Rahul; Behl, Jyotsna; Gupta, Neelam; Gupta, S.C.; Ahlawat, S.P.S.;
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The capability of microsatellite markers for individual identification and their potential for breed assignment of individuals was evaluated in two Indian horse breeds. The strength of these individual assignment methods was also evaluated by increasing the number of loci in increments of five. The probability of identity of two random horses from the two breeds at all twenty five studied loci was as low as showing their suitability to distinguish between individual horses and their products. In the phylogenetic approach for individual assignment using Nei's genetic distances, 10.81% of horses associated with breed other than the major cluster of the source breed horses when all twenty five microsatellite loci were implemented. Similar results were obtained when the maximum likelihood approach for individual assignment was used. Based on these results it is proposed that, although microsatellite markers may prove very useful for individual identification, their utility for breed assignment of horses needs further evaluation.
Individual Identification;Breed Assignment;Microsatellite Markers;Indian Horses;
 Cited by
Aberle, K. S., H. Hamann, C. Drogemuller and O. Distl. 2004. Genetic diversity in Germen drought horse breeds compared with a group of primitive, riding and wild horses by means of microsatellite DNA markers. Anim. Genet. 35:270-277. crossref(new window)

Banks, M. A. and W. Eichert. 2001. WHICHRUN, version 4.1, a computer programme for population assignment based on multilocus genotype data. Bodega Marine Laboratory, University of California at Davies. Bodega Bay, CA, USA.

Behl, R., R. Kaul, N. Sheoran, J. Behl, M. S. Tantia and R. K. Vijh. 2002. Genetic identity of two Indian pig types using microsatellite markers. Anim. Genet. 33:158-159. crossref(new window)

Bhat, P. N., P. P. Bhat, B. U. Khan, O. B. Goswami and B. Singh. 1981. Animal genetic resources of India. National Dairy Research Institute Press, Karnal. pp. 87-88.

Bjornstad, G. and K. H. Roed. 2001. Breed demarcation and potential for breed allocation of horses assessed by microsatellite markers. Anim. Genet. 32:59-65. crossref(new window)

Bjornstad, G. and K. H. Roed. 2002. Evaluation of factors affecting breed assignment precision using microsatellite data from horse breeds and simulated breed crosses. Anim. Genet. 33:264-270. crossref(new window)

Bjornstad, G., E. Gunby and K. H. Roed. 2000. Genetic structure of Norwegian horse breeds. J. Anim. Breed. Genet. 117:307-317. crossref(new window)

Bjornstad, G., N. O. Nilsen and K. H. Roed. 2003. Genetic relationship between Mongolian and Norwegian horses? Anim. Genet. 34:55-58. crossref(new window)

Blott, S. C., J. L. Williams and C. S. Haley. 1999. discriminating among cattle breeds using genetic markers. Heredity. 82:613-619. crossref(new window)

Bokonyi, S. 1984. Horse. In: Evolution of Domesticated Animals (Ed. I. L. Mason). Longman, London. pp. 162-173.

Bowling, A. T., M. L. Eggleston-Stott, G. Byrns, R. S. Clark, S. Dileanis and E. Wictum. 1997. Validation of microsatellite markers for routine horse parentage testing. Anim. Genet. 28:247-252. crossref(new window)

Buchanan, F. C., L. J. Adams, R. P. Littlejohn, J. F. Maddox and A. M. Crawford. 1994. Determination of evolutionary relationships among sheep breeds using microsatellites. Genomics. 22:397-403. crossref(new window)

Canon, J., M. L. Checa, C. Carleos, J. L. Vega-Pla, M. Vallejo and S. Dunner. 2000. The genetic structure of Spanish celtic horse breeds inferred from microsatellite data. Anim. Genet. 31:39-48. crossref(new window)

Cho, G. J. and B. W. Cho. 2004. Microsatellite DNA typing using 16 markers for parentage verification of the Korean native horse. Asian-Aust. J. Anim. Sci. 17:750-754. crossref(new window)

Cornuet, J.-M. S. Piry, G. Luikart, A. Estoup and M. Solignac. 1999. New methods employing multilocus genotypes to select or exclude populations as origins of individuals. Genet. 153:1989-2009.

Felsenstein, J. 2004. PHYLIP, version 3.6b, phylogeny inference package. Department of Genome science, University of Washington, Seattle.

Jin, L. and R. Chakraborty. 1994. Estimation of genetic distance and coefficient of gene diversity from single-probe multilocus DNA fingerprinting data. Mol. Biol. Evol. 11:120-127.

Katoch, S. P. K. Dogra, Y. P. Thakur and K. Gupta. 2004. Phenotypic characterization of Spiti horse in its breeding tract-body measurements. Centaur. 20:45-48

MacHugh, D. E., R. T. Loftus, P. Cunningham and D. G. Bradley. 1998. Genetic structure of seven cattle breeds assessed using 20 microsatellite markers. Anim. Genet. 29:333-340. crossref(new window)

Marklund, S., H. Ellegren, S. Erikson, K. Sandberg and L. Anderson. 1994. Parentage testing and linkage analysis in the horse using a set of highly polymorphic microsatellites. Anim. Genet. 25:19-23. crossref(new window)

Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genet. 89:583-590.

Page, R. D. M. 1996. TREEVIEW: An application to display phylogenetic trees on personal computers. Computer Appl. Biosci. 12:357-358.

Pundir, R. K. 2001. Physical and morphological characteristics of Spiti horses. Indian J. Anim. Sci. 71:381-382.

Singh, M. K., M. P. Yadav and N. T. .Mehta. 2002. Breed characteristics of Marwari and Kathiawari horses. Indian J. Anim. Sci. 72:319-323.

Tozaki, T., H. Kokoi, S. Mashima, K. I. Hirota, T. Hasegawa, N. Ishida, N. Miura, N. H. Choi-Miura and M. Tomita. 2001. Population study and validation of paternity testing for Thoroughbred horses by 15 microsatellite loci. J. Vet. Med. Sci. 63:1191-1197. crossref(new window)

Tozaki, T., N. Takezaki, T. Hasegawa, N. Ishida, M. Kurusawa, N. Saitou and H. Mukoyama. 2003. Microsatellite variation in Japanese and Asian horses and their phylogenetic relationship using a European horse outgroup. J. Hered. 94:374-380. crossref(new window)

Yang, L., S. H. Zhao, K. Li, Z. Z. Peng and G. W. Montgomery. 1999. Detemination of relationship among five indigenous Chinese goat breeds with six microsatellite markers. Anim. Genet. 30:452-455. crossref(new window)

Yeh, F. C., R. Yang and T. Boyle. 1999. POPGENE, version 1.31, a microsoft windows based freeware for population genetics analysis. University of Alberta, Alberta, Canada.