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Effects of Ovarian Morphology and Culture Vessel on In vitro Development and Cell Number in Embryos of Korean Native Cows
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 Title & Authors
Effects of Ovarian Morphology and Culture Vessel on In vitro Development and Cell Number in Embryos of Korean Native Cows
Park, Yong-Soo; Kim, Jae-Myeoung;
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The main purpose of this study was to improve the efficiency and quality of in vitro embryo production in Korean Native Cows (KNC). We examined the effects of ovarian morphologies (Experiment 1) and the culture vessel (Experiment 2) on in vitro maturation (IVM). We measured the subsequent development rates and cell numbers of blastocysts. In Experiment 1, the ovaries of KNC were divided into six groups, based on follicle and corpus luteum (CL) morphology. The development rates to the 2- and 8-cell stages were similar among the six groups. The development rates to blastocyst stages were significantly higher in the group without a CL or follicle (WOCL/F) than in the groups with follicular cysts (FCs), regressive CLs (RCLs) or cystic CLs (CCLs) (p<0.05). The cell number of the inner cell mass (ICM) of blastocysts in the FCs and RCLs groups, and the number of cells in the trophectoderm (TE) in the WOCL/F group, FCs, growing CLs (GCLs) and RCLs were significantly higher than in other groups (p<0.05). The total cell number (TCN) in the WOCL/F, FC and RCL groups was also significantly higher than in other groups (p<0.05). The ICM cell number/TCN ratio was significantly higher in the FC and RCL groups than in the GCL and DF groups (p<0.05). In Experiment 2, oocyte IVM was carried out in culture dishes, in 0.25- or 0.5-ml straws used for freezing sperm. The development rate to the 2-cell stage was significantly higher in the 0.5-ml straw group than in the 0.25-ml straw group. The development rates to the blastocyst stage were similar in the dish and the two straw groups. There were no differences in the cell numbers of ICM, TE or TCN or ICM cell number/TCN ratios between groups.
Bovine;In vitro Maturation (IVM);Ovarian Morphology;Straw;Cell Uumber;
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Asian-Australasian Journal of Animal Sciences, 2007. vol.20. 12, pp.1820-1826 crossref(new window)
Bavister, B. D., M. L. Leibfried and G. Lieberman. 1983. Development of preimplantation embryos of golden hamster in a defined culture medium. Biol. Reprod. 28:235-247. crossref(new window)

Boone, W. R. and S. S. Shapiro. 1990. Quality control in the in vitro fertilization laboratory. Theriogenol. 33:23-50. crossref(new window)

Enright, B. P., P. Lonergan, A. Dinnyes, T. Fair, F. A. Ward, X. Yang and M. P. Boland. 2000. Culture of in vitro produced bovine zygotes in vitro vs. in vivo: Implications for early embryo development and quality. Theriogenol. 54:659-673. crossref(new window)

Fukui, Y. and H. Ono. 1989. Effects sera, hormonse and granulosa cells added to culture medium for in vitro maturation, fertilization, cleavage and development of bovine oocytes. J. Reprod. Fertil. 86:501-506. crossref(new window)

Gardner, D. K. and H. J. Leese. 2000. Assessment of embryo metabolism and viability. In; Handbook of in vitro fertilization (Ed. A. O. Trounson, D. K. Gardner). CRC press, New York, pp. 347-372.

Hammitt, D. G., D. L. Walker, C. H. Syrop, T. M. Miler and M. R. Bennett. 1991. Treatment of sever mala-factor infertility with high concentrations of motile sperm by microinsemination in embryo cryopreservation straws. J. In vitro Fertil. Embryo Transfer 8:101-110. crossref(new window)

Hernandez-Ledezma, J. J., C. Villanueva, J. D. Sikes and H. M. Kubisch. 1996. Increasing the rate of blastocyst formation and hatching from in vitro-produced bovine zygotes. Theriogenol. 46:961-969. crossref(new window)

Iwasaki, S., N. Yoshiba, H. Ushijima, S. Watanabe and T. Nakahara. 1990. Morphology and proportion of inner cell mass of bovine blastocysts fertilized in vitro and in vivo. J. Reprod. Fertil. 90:279-284. crossref(new window)

Kamishita, H., M. Takagi, Y. H. Choi, M. P. B. Wijayagunawardane, K. Miyazawa and K. Sato. 1999. Development of in vitro matured and fertilized bovine embryos cocultured with bovine oviductal epithelial cells obtained from oviducts ipsilateral to cystic follicles. Anim. Reprod. Sci. 56:201-209. crossref(new window)

Kang, H. J., C. S. Lee, I. H. Kim, I. P. Mo, K. C. Lee and G. H. Suh. 2006. Evaluation of functional status of ovarian cysts in slaughtered Korean native cows. Kor. J. Emb. Trans. 21:59-67.

Ko, J., J. Kastelic, M. Del Campo and O. Ginther. 1991. Effect of a dominant follicle on ovarian follicular dynamics during oestrus cycle in heifers. J. Reprod. Fertil. 91:511-519. crossref(new window)

Leibfried, L. and N. L. First. 1979. Characterization of bovine follicular oocytes and their ability to mature in vitro. J. Anim. Sci. 48:76-86.

Lequarre, A. S., C. Vigneron, F. Ribaucour, P. Holm, I. Donnay, R. Dalbies-Tran, H. Callesen and P. Mermillod. 2005. Influence of antral follicle size on oocyte characteristics and embryo development in the bovine. Theriogenol. 63:841-859. crossref(new window)

Li, Y. H., W. Ma, M. Li, L. Hou, L. H. Jiao and W. H. Wang. 2003. Reduced polyspermic penetration in porcine oocytes inseminated in a new in vitro fertilization (IVF) system: Straw IVF. Biol. Reprod. 69:1580-1585. crossref(new window)

Linder, G. M. and W. W. Wright. 1983. Bovine embryo morphology and evaluation. Theriogenol. 20:407-416. crossref(new window)

Lonergan, P., P. Monaghan, D. Rizos, M. P. Boland and I. Gordon. 1994. Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization, and culture in vitro. Mol. Reprod. Dev. 37:48-53. crossref(new window)

Lonergan, P., D. Rizos, A. Gutierrez-Adan, T. Fair and M. P. Boland. 2003. Oocyte and embryo quality: Effect of origin, culture conditions and gene expression patterns. Reprod. Dom. Anim. 38:259-267. crossref(new window)

Machatkova, M., E. Jokesova and V. Dvoracek. 1996. Developmental competence of bovine embryos derived from oocytes collected at various stages of the estrus cycle. Theriogenol. 45:801-810. crossref(new window)

Papaioannou, V. E. and K. M. Ebert. 1988. The preimplantation pig embryo: Cell number and allocation to trophectoderm and inner cell mass of the blastocyst in vivo and in vitro. Development 102:793-803.

Park, Y. S., S. S. Kim, S. H. Choi, N. C. Park, M. D. Byun and H. D. Park. 2004. Effects of amino acid in in-vitro maturation medium on nuclear maturation and embryo development of Korean native cow. Reprod. Dev. Biol. 28:29-36.

Park, Y. S., S. S. Kim, J. M. Kim, H. D. Park and M. D. Byun. 2005. The effects of duration of in vitro maturation of bovine oocytes on subsequent development, quality and transfer of embryos. Theriogenol. 64:123-134. crossref(new window)

Ranoux, C. and M. M. Seibel. 1990. New techniques in fertilization: intravaginal culture and microvolume straw. J. In vitro Fertil. Embryo. Transfer. 7:6-8. crossref(new window)

Rieger, D. and N. M. Loskutoff. 1994. Changes in the metabolism of glucose, pyruvate, glutamine and glycine during maturation of cattle oocytes in vitro. J. Reprod. Fertil. 100:257-262. crossref(new window)

Roberts, R., S. Franks and K. Hardy. 2002. Culture environment modulates maturation and metabolism of human oocytes. Hum. Reprod. 17:2950-2956. crossref(new window)

Rosenkrans, C. F. Jr, G. Q. Zeng, G. T. McNamara, P. K. Schoff. and N. L. First. 1993. Development of bovine embryos in vitro as affected by energy substrates. Biol. Reprod. 49:459-462. crossref(new window)

Son, C. H., B. K. Kang, H. S. Choi, W. H. Lim, H. G. Kang, K. S. Oh, J. B. Shin and G. H. Suh. 1999. Development of differential diagnosis and treatment method of reproductive disorders using ultrasonography in cows III. Differential diagnosis between developing and regressing corpus luteum. Kor. J. Vet. Clin. Med. 16:118-127.

van der Ven, H. H., K. Hoebbel, S. Al-Hasani, K. Diedrich and D. Kerbs. 1989. Fertilization of oocytes in capillary tubes with very small numbers of spermatozoa. Hum. Reprod. 4:72-76.

van Soom, A., M. Boerjan, M. T. Ysebaert and A. de Kruif. 1996. Cell allocation to the inner cell mass and the trophectoderm in bovine embryos cultured in two different media. Mol. Reprod. Dev. 45:171-182. crossref(new window)

Varisanga, M. D., C. Sumantri, M. Murakami, M. Fahrudin and T. Suzuki. 1998. Morphological classification of the ovaries in relation to the subsequent oocyte quality for IVF-produced bovine embryos. Theriogenol. 50:1015-1023. crossref(new window)

Watson, A. J., P. Sousa, A. Caveney, L. C. Barcroft, D. Natale, J. Urquhart and M. E. Westhusin. 2000. Impact of bovine oocyte maturation media on oocyte transcript levels, blastocyst development, cell number, and apoptosis. Biol. Reprod. 62:355-364. crossref(new window)