JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effect of Tannins in Acacia nilotica, Albizia procera and Sesbania acculeata Foliage Determined In vitro, In sacco, and In vivo
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Tannins in Acacia nilotica, Albizia procera and Sesbania acculeata Foliage Determined In vitro, In sacco, and In vivo
Alam, M.R.; Amin, M.R.; Kabir, A.K.M.A.; Moniruzzaman, M.; McNeill, D.M.;
  PDF(new window)
 Abstract
The nutritive value and the effect of tannins on the utilization of foliage from three commonly used legumes, Acacia nilotica, Albizia procera, and Sesbania acculeata, were determined. Three mature rumen-fistulated bullocks were used to study in sacco degradability and twelve adult sheep were randomly allocated on the basis of live weight to 4 groups of 3 in each to study the in vivo digestibility of the foliages. In all foliages, the contents of crude protein (17 to 24% of DM) were high. Fibre was especially high in Albizia (NDF 58.8% of DM vs. 21% in Sesbania and 15.4% in Acacia). Contents of both hydrolysable (4.4 to 0.05%) and condensed tannins (1.2 to 0.04%) varied from medium to low in the foliages. Acacia contained the highest level of total phenolics (20.1%), protein precipitable phenolics (13.2%) and had the highest capacity to precipitate protein (14.7%). Drying in shade reduced the tannin content in Acacia and Albizia by 48.6 and 69.3% respectively. The foliages ranked similarly for each of the different methods used to estimate tannin content and activity. Acacia and Sesbania foliage was highly degradable (85-87% potential degradability of DM in sacco), compared to Albizia (52%), indicating a minimal effect of tannins in Acacia and Sesbania. Yet, in vitro, the tannins in the Acacia inhibited microbial activity more than those in Albizia and Sesbania. Following the addition of polyethylene glycol to neutralise the tannins, gas production and microbial growth increased by 59% and 0.09 mg RNA equiv./dg microbial yield respectively in the Acacia, compared to 16-17% and 0.06 mg RNA equiv./dg microbial yield in the other foliages. There was a trend for low in vivo apparent digestibility of N in the Acacia (43.2%) and Albizia (44.2%) compared to the Sesbania (54.5%) supplemented groups. This was likely to be due to presence of tannins. Consistent with this was the low N retention (0.22 and 0.19 g N/g NI) in sheep supplemented with Acacia and Albizia compared to that for the Sesbania (0.32). Similarly, a trend for poor microbial N yield was observed in sheep fed these foliages. Across the foliages tested, an increase in tannin content was associated with a reduction in ruminal fermentation, N digestibility and N retention. For overall nutritive value, Sesbania proved to be the superior forage of the three tested.
 Keywords
Acacia;Albizia;Sesbania;Polyphenolics;Digestibility;Purine Derivative;
 Language
English
 Cited by
1.
In vitro Nutrient Digestibility, Gas Production and Tannin Metabolites of Acacia nilotica Pods in Goats,;;

Asian-Australasian Journal of Animal Sciences, 2008. vol.21. 1, pp.59-65 crossref(new window)
 References
1.
AOAC. 1990. Official Methods of Analysis. 15th ed., Association of Official Analytical Chemists, Washington, DC.

2.
Abdulrazak, S. A., R. W. Muinga, W. Thorpe and E. R. Orskov. 1997. Supplementation with Gliricidia Sepium and Leucaena leucocephala on voluntary feed intake, digestibility, rumen fermentation and live-weight of crossbred steers offered Zea mays stover. Livestock Prod. Sci. 49:53-62. crossref(new window)

3.
Ahn, J. H., B. M. Robertson, R. Elliott, R. C. Gutteridge and C. W. Ford. 1989. Quality assessment of tropical browse legumes tannin content and protein degradation. Anim. Feed Sci. Technol. 27:147-156. crossref(new window)

4.
Alam, M. R. 1998. Potential use legume tree leaves as forage in Bangladesh. In: Nitrogen fixing trees for fodder production. (Ed. J. N. Daniel and J. M. Roshetko). Winrock International, USA, pp. 205-211.

5.
Barry, T. N. and S. J. Duncan. 1984. The role of condensed tannins in the nutritional value of L. pedunculatus for sheep. 1. Voluntary intake, Br. J. Nutr. 51:485-491. crossref(new window)

6.
Barry, T. N. and T. R. Manley. 1984. The role of condensed tannins in the nutritional value of L. pedunculatus for sheep. 2. Quantitative digestion of carbohydrates and proteins. Br. J. Nutr. 51:493-504. crossref(new window)

7.
Barry, T. N., M. D. McNeill and W. C. McNabb. 2001. Plant secondary compounds; their impact on nutritive value and upon animal production. In: Proceedings of the XIX International Grassland Congress. Brazilian Society of Animal Husbandry, Piracicaba., pp. 445-452.

8.
Bhargava, P. K. and E. R. Orskov. 1987. Manual for the use of nylon bag technique in the evaluation of foodstuffs. Rowett Research Institute, UK.

9.
Chen, X. B., J. D. F. Mathieson, Dev. Hovell and P. J. Reeds. 1990. Measurement of purine derivatives in urine of ruminants using automated methods. J. Sci. Food Agric. 53:23-33. crossref(new window)

10.
Dawra, R. K., H. P. S. Makkar and B. Singh. 1988. Protein binding capacity of microquantities of tannins. Anal. Biochem. 170:50- 53. crossref(new window)

11.
Evaitayani, L., A. Warly, A. Fariani, T. Ichinohe and T. Fujihara 2004. Study on nutritive value of tropical forages in North Sumatra, Indonesia. Asian-Aust. J. Anim. Sci. 17:1518-1523.

12.
Faichney, G. J. and G. A. White. 1983. Methods for the analysis of feeds eaten by ruminants. CSIRO, Australia.

13.
Hagerman, A. E. 1987. Radial diffusion method for determining tannins in plant extracts. J. Chem. Ecol. 13:437-449. crossref(new window)

14.
Hagerman, A. E., M. E. Rice and N. T. Ritchard. 1998. Mechanisms of protein precipitation for two tannins, pentagalloyl glucose and epicatechin(16) (4>8) catechin (procyanidin). J. Agric. Food Chem. 46:2590-2595. crossref(new window)

15.
Inoue, H. and A. E. Hagerman. 1988. Determination of gallotannins with rhodanine. Anal. Biochem. 169:363-369. crossref(new window)

16.
IAEA. 1997. Estimation of rumen microbial protein production from purine derivatives in urine. A Laboratory Manual. International Atomic Energy Agency Technical Document, IAEA-TECDOC-945, pp. 15-37.

17.
Kumar, R. 1992. Anti-nutritional factors, the potential risks of toxicity and methods to alleviate them. In: Legume trees and other fodder trees as protein sources for livestock (Ed. A. Speedy and P. L. Pugliese) FAO Animal Production and Health Paper 102. Food and Agriculture Organisation of the United Nations, Rome.

18.
Lawry, J. B. 1990. Toxic factors and problems: Methods of alleviating them in animals. In: Shrubs and Tree fodders for Farm (Ed. C. Devendra). IDRC, Canada, pp. 76-88.

19.
Makkar, H. P. S. 2003. Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Rum. Res. 49:241-256.

20.
Makkar, H. P. S., R. K. Dawra and B. Singh. 1988. Determination of both tannin and protein in a tannin-protein complex. J. Agric. Food Chem. 36:523-525. crossref(new window)

21.
Makkar, H. P. S., K. D. Rajinder and B. Singh. 1991. Tannin levels in leaves of some Oak species at different stages of maturity. J. Sci. Food Agric. 54:513-519. crossref(new window)

22.
Makkar, H. P. S., M. Bluemmel, N. K. Borowy and K. Becker. 1993. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. J. Sci. Food Agric. 61:161-165. crossref(new window)

23.
Makkar, H. P. S., M. Blummel and K. Becker. 1995. Formation of complexes between polyvinylpyrrolidone and polyethylene glycol with tannins and their implications in gas production and true digestibility in in vitro techniques. Br. J. Nutr. 73:897- 913. crossref(new window)

24.
Makkar, H. P. S. and K. Becker. 1996. A bioassay for phenols (Tannins). Polyphenols Communications, Bordeaux (France), 96:15-18.

25.
Makkar, H. P. S. and K. Becker. 1999. Purine quantification in digesta from ruminants by spectrophotometric and HPLC methods. Br. J. Nutr. 81:107-113.

26.
Mangan, J. L. 1988. Nutritional effects of tannins in animal feeds. Nutr. Res. Rev. 1:209-231. crossref(new window)

27.
Manke, K. H., L. Raab, A. Salewski, H. Steingass, D. Fritz and W. Schneider. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. J. Agric. Sci. (Camb) 93:217-222. crossref(new window)

28.
McNeill, D. M. N. Osborne, M. K. Komolong and D. Nankervis. 1998. Condensed tannins in the Genus Leucaena and their nutritional significance for ruminants. In: Leucaena- Adaptation, Quality and Farming Systems (Ed. H. M. Shelton, R. C. Gutteridge, B. F. Multen and R. A. Bray). ACIAR Proceedings No. 86, Canberra, Australia, pp. 205-214.

29.
Norton, B. W. 1999. The significance of tannins in tropical animal production. In: Tannins in livestock and human nutrition (Ed. J. D. Brooker). ACIAR Proceedings no. 92, Canberra, Australia, pp. 14-23.

30.
Norton, B. W. and H. H. Ahn. 1997. A comparison of fresh and dried Calliandra calothyrsus supplements for sheep given a basal diet of barley straw. J. Agric. Sci. 129:485-494. crossref(new window)

31.
Osuga, I. M., S. A. Abdulrazzak, T. Ichinohe and T. Fujihara. 2005. Chemical composition, degradation characteristics and efeect of tannins on digestibility of some browse species from Kenya harvested during the wet season. Asian-Aust. J. Anim. Sci. 18:54-60.

32.
Perez-Maldonado, R. A. and B. W. Norton. 1996. The effects of condensed tannins from Desmodium intortum and Calliandra calothyrsus on protein and carbohydrate digestion in sheep and goats. Br. J. Nutr. 76:515-533. crossref(new window)

33.
Pritchard, D. A., P. R. Martin and P. K. O'Rourke. 1992. The role of condensed tannins in the nutritional value of mulga (Acacia aneura) for sheep. Aust. J. Agric. Res. 43:1739-1746. crossref(new window)

34.
Porter, L. J., L. N. Hrstich and B. G. Chen. 1986. The conversion of procyanidins and prodelphinidins to cyaniding and delphinidin. Phytochemistry 25:223-230. crossref(new window)

35.
Reed, J. D., H. Soller and A. Woodward. 1990. Fodder tree and straw diets for sheep: intake, growth, digestibility and the effect of phenolics on nitrogen utilization. Anim. Feed Sci. Technol. 30:39-50. crossref(new window)

36.
Statistical Analysis Systems Institute, 1988. Procedures Guide for Personal Computers. Version 6.03, SAS Institute Inc., Cary, NC.

37.
Stewart, J. L., A. L. Dansdon, M. Kass, S. Ortiz Lopez, A. Larbi, S. Premaratne, B. Tangendjaja, E. Wina and J. E. Vargas. 1998. Acceptability, intake, digestibility and live weight gain in small ruminants. Anim. Feed Sci. Technol. 75:111-124. crossref(new window)

38.
Van Soest, P. J. 1994. Nutritional Ecology of the Ruminant, Second Edition. Comstock Pub., Ithaca.

39.
Waghorn, G. C. 1990. Effects of condensed tannin on protein digestion and nutritive value of fresh herbage. Proceedings of the Australian Society of Animal Production, 18:412-415

40.
Waghorn, G. C., I. D. Shelton, W. C. McNabb and S. H. McCutcheon. 1994. Effects of condensed tannins in L. pedunculatus on its nutritive value for sheep. 2. Nitrogenous aspects. J. Agric. Sci. 123:109-119. crossref(new window)

41.
Wang, Y., G. B. Douglas, G. C. Waghorn, T. N. Barry, A. G. Foote and R. W. Purchas. 1996. Effect of condensed tannins upon the performance of lambs grazing L. corniculatus and lucerne. J. Agric. Sci. 126:87-98. crossref(new window)

42.
Wina, E., B. Tangedjaja and B. Palmer. 1999. The effect of drying conditions on condensed tannin estimates in calliandra calothyrsus. In: Tannins in livestock and human nutrition. (Ed. J. D. Brooker). ACIAR Proceedings no. 92, Canberra, Australia, pp. 106-110.