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Functional Amino Acids and Fatty Acids for Enhancing Production Performance of Sows and Piglets
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 Title & Authors
Functional Amino Acids and Fatty Acids for Enhancing Production Performance of Sows and Piglets
Kim, Sung Woo; Mateo, Ronald D.; Yin, Yu-Long; Wu, Guoyao;
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 Abstract
The growth and health of the fetus and neonate are directly influenced by the nutritional and physiological status of sows. Sows are often under catabolic conditions due to restrict feeding program during pregnancy and low voluntary feed intake during lactation. The current restrict feeding program, which aims at controlling energy intake during gestation, results in an inadequate supply of dietary protein for fetal and mammary gland growth. Low voluntary feed intake during lactation also causes massive maternal tissue mobilization. Provision of amino acids and fatty acids with specific functions may enhance the performance of pregnant and lactating sows by modulating key metabolic pathways. These nutrients include arginine, branched-chain amino acids, glutamine, tryptophan, proline, conjugated linoleic acids, docosahexaenoic acid, and eicosapentaenoic acid, which can enhance conception rates, embryogenesis, blood flow, antioxidant activity, appetite, translation initiation for protein synthesis, immune cell proliferation, and intestinal development. The outcome is to improve sow reproductive performance as well as fetal and neonatal growth and health. Dietary supplementation with functional amino acids and fatty acids holds great promise in optimizing nutrition, health, and production performance of sows and piglets. (Supported by funds from Texas Tech, USDA, NLRI-RDA-Korea, and China NSF).
 Keywords
Amino Acids;Fatty Acids;Growth;Health;Neonate;Pigs;Reproduction;Sows;
 Language
English
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 References
1.
Anderson, M. and K. L. Fritsche. 2002. (n-3) Fatty acid and infectious disease resistance. J. Nutr. 132:3566-3576.

2.
Arbuckle, L. D. and S. M. Innis. 1993. Docohexaenoic acid is transferred through maternal diet to milk and to tissues of naturally milk fed piglets. J. Nutr. 123:1668-1675.

3.
Arntzen, K. J., O. L. Brekke, L. Vatten and R. Austgulen. 1998. Reduced production of PGE2 and PGF2 alpha from decidual cell cultures supplemented with N-3 polyunsaturated fatty acids. Prostaglandins Other Lipid Mediat. 56:183-195. crossref(new window)

4.
Baker, D. H. 1997. Ideal amino acid profiles for swine and poultry and their applications in feed formulation. Biokyowa Tech. Rev. 9:1-24.

5.
Ban, H., K. Shigemitsu, T. Yamatsuji, M. Haisa, T. Nakajo, M. Takaoka, T. Nobuhisa, M. Gunduz, N. Tanaka and Y. Naomoto. 2004. Arginine and leucine regulate p70 S6 kinase and 4E-BP1 in intestinal epithelial cells. Int. J. Mol. Med. 13:537-543.

6.
Bazinet, R. P., E. G. McMillan and S. C. Cunnane. 2003. Dietary alpha-linolenic acid increases the n-3 PUFA content of sows milk and the tissues of the suckling piglet. Lipids 38:1045-1049. crossref(new window)

7.
Brazle, A. E., B. J. Johnson, E. C. Titgemeyer, S. K. Webel and D. L. Davis. 2005. Fatty acid composition of the porcine conceptus in response to maternal omega-3 fatty acid supplementation. J. Anim. Sci. 83 (Suppl. 1):410(Abstr.).

8.
Brazle, A. E., B. J. Johnson, E. C. Titgemeyer, S. K. Webel and D. L. Davis. 2006. Fatty acid composition of the pig conceptus and endometrium in response to maternal omega-3 fatty acid supplementation. J. Anim. Sci. 84(Suppl. 2):77(Abstr.).

9.
Bressner, G., S. W. Kim and R. A. Easter. 2000. Effects of spritweaning on the performance of primiparous sows and the performance nursing pigs. J. Anim. Sci. 78 (Suppl. 1): 240(Abstr.)

10.
Bronte, V. and P. Zanovello. 2005. Regulation of immune responses by L-arginine metabolism. Nature Rev. Immunol. 5:641-654. crossref(new window)

11.
Brosnan, J. T. 2001. Amino acids, then and now - a reflection on Sir Hans Kreb's contribution to nitrogen metabolism. IUBMB Life 52:265-270. crossref(new window)

12.
Coeffier, M., S. Claeyssens, B. Hecketsweiler, A. Lavoinne, P. Ducrotte and P. Dechelotte. 2003. Enteral glutamine stimulates protein synthesis and decreases ubiquitin mRNA level in human gut mucosa. Am. J. Physiol. 285:G266-G273.

13.
Cole, G. M., G. P. Lim, F. Yang, B. Tter, A. Begum, Q. Ma, M. E. H. White and S. A. Frautschy. 2005. Prevention of Alzheimers disease: Omega 3 fatty acid and phenolic anti-oxidant interventions. Neurobiol. Aging 26S:S133-S136.

14.
Cori, B., M. Rhoads, R. J. Harrell, A. T. Blikslager, O. T. Phillips, L. A. Gatlin, X. M. Niu and J. Odle. 2005. Rotaviral enteritis stimulates ribosomal p70 s6 kinase and increases intestinal protein synthesis in neonatal pigs. FASEB J. 19:A976.

15.
Crawford, M. A. 2000. Placental delivery of arachidonic and docosahexaenoic acids: Implication for the lipid nutrition of preterm infants. Am. J. Clin. Nutr. 71:275S-284S.

16.
Crawford, M. A., K. Casteloe, K. Ghebremeskel, A. Phylactos, L. Skirvin and F. Stacey. 1997. Are deficits of arachidonic and docosahexaenoic acids responsible for the neural and vascular complications of preterm babies? Am. J. Clin. Nutr. 66:1032S-1041S.

17.
Curthoys, N. P. and M. Watford. 1995. Regulation of glutaminase activity and glutamine metabolism. Annu. Rev. Nutr. 15:133- 159. crossref(new window)

18.
Delion, S., S. Chalon, J. Hérault, D. Guilloteau, J. C. Besnard and G. Durand. 1994. Chronic dietary -linolenic acid deficiency alters dopaminergic and serotoninergic neurotransmission in rats. J. Nutr. 124:2466-2476.

19.
Dourmad, J. Y., M. Etienne, A. Prunier and J. Noblet. 1994. The effect of energy and protein intake of sows on their longevity: a review, Livest. Prod. Sci. 40:87-97. crossref(new window)

20.
Elias, S. L. and S. M. Innis. 2001. Newborn infant plasma trans, conjugated linoleic, n-6, n-3 fatty acids are related to maternal plasma fatty acids, length of gestation and birth weight and length. Am. J. Clin. Nutr. 73:807-814.

21.
Escobar, J., J. W. Frank, A. Suryawan, H. V. Nguyen, S. R. Kimball, L. S. Jefferson and T. A. Davis. 2005. Physiological rise in plasma leucine stimulates muscle protein synthesis in neonatal pigs by enhancing translation initiation factor activation. Am. J. Physiol. 288:E914-E921.

22.
Escobar, J., J. W. Frank, A. Suryawan, H. V. Nguyen, S. R. Kimball, L. S. Jefferson and T. A. Davis. 2006. Regulation of cardiac and skeletal muscle protein synthesis by individual branched-chain amino acids in neonatal pigs. Am. J. Physiol. 290:E612-E621.

23.
Esteban, S., C. Nicolaus, A. Garmundi, R. E. Rial, A. B. Rodriguez, E. Ortega and C. B. Ibars. 2004. Effect of orally administered L-tryptophan on serotonin, melatonin, and the innate immune response in the rat. Mol. Cell. Biochem. 267:39-46. crossref(new window)

24.
Field, C. J., I. R. Johnson and P. D. Schley. 2002. Nutrients and their role in host resistance to infection. J. Leuk. Biol. 71:16- 32.

25.
Flynn, N. E. and G. Wu. 1996. An important role for endogenous synthesis of arginine in maintaining arginine homeostasis in neonatal pigs. Am. J. Physiol. 271:R1149-R1155.

26.
Flynn, N. E., C. J. Meininger, T. E. Haynes and G. Wu. 2002. The metabolic basis of arginine nutrition and pharmacotherapy. Biomed. Pharmacother. 56:427-438. crossref(new window)

27.
Flynn, N. E., D. A. Knabe, B. K. Mallick and G. Wu. 2000. Postnatal changes of plasma amino acids in suckling pigs. J. Anim. Sci. 78:2369-2375

28.
Frank, J. W., J. Escobar, H. V. Nguyen, S. C. Jobgen, T. A. Davis, and G. Wu. 2006. Oral N-carbamylglutamate supplementation increases growth rate in sow-reared piglets. FASEB J. A266.3.

29.
Fritsche, K. L., D. W. Alexander, N. A. Cassity and S. C. Huang. 1993b. Maternally-supplied fish oil alters piglet immune cell fatty acid profile and eicosanoid production. Lipids 28:677-682. crossref(new window)

30.
Fritsche, K. L., S. C. Huang and N. A. Cassity. 1993a. Enrichment of omega-3 fatty acids in suckling pigs by maternal dietary fish oil supplementation. J. Anim. Sci. 71:1841-1847.

31.
Fu, W., T. E. Haynes, R. Kohli, J. Hu, W. Shi, T. E. Spencer, R. J. Carroll, C. J. Meininger and G. Wu. 2005. Dietary supplementation with L-arginine reduces fat mass in Zucker diabetic fatty rats. J. Nutr. 135:714-721.

32.
Fumarola, C., S. La Monica and G. G. Guidotti. 2005. Amino acid signaling through the mammalian target of rapamycin (mTOR) pathway: Role of glutamine and of cell shrinkage. J. Cell. Physiol. 204:155-165. crossref(new window)

33.
Gao, H., T. E. Spencer, G. Wu, G. A. Johnson and F. W. Bazer. 2006. mTOR signaling mechanisms in the ovine uterus. Biol. Reprod. Special Issue p. 123.

34.
Grimble, R. F. 1998. Modification of inflammatory aspects of immune function by nutrients. Nutr. Res. 18:1297-1317 crossref(new window)

35.
Grimble, R. F. 2001. Nutritional modulation of immune function. Proc. Nutr. Soc. 60:389-397 crossref(new window)

36.
Ha, E. M., C. T. Ch, Y. S. Bae and W. J. Lee. 2005. A direct role for dual oxidase in Drosophila gut immunity. Science 310:847-850. crossref(new window)

37.
Harris, W. S., W. E. Connor and S. L. Lindsey. 1984. Will dietary $\omega$-3 fatty acids change composition of human milk? Am. J. Clin. Nutr. 40:780-785.

38.
Hornstra, G. 2000. Essential fatty acids in mothers and their neonates. Am. J. Clin. Nutr. 71:1262S-1269S.

39.
Innis, S. M. 1991. Essential fatty acids in growth and development. Prog. Lipid Res. 30:39-103. crossref(new window)

40.
Innis, S. M. and S. L. Elias. 2003. Intakes of essential n-6 and n-3 polyunsaturated fatty acids among pregnant Canadian women. Am. J. Clin. Nutr. 77:473-478.

41.
Ji, F., G. Wu, J. R. Blanton and S. W. Kim. 2005. Weight and compositional changes in pregnant gilts and its implication to nutrition. J. Anim. Sci. 83:366-375.

42.
Jobgen, W. S., S. K. Fried, W. J. Fu, C. J. Meininger and G. Wu. 2006. Regulatory role for the arginine-nitric oxide pathway in energy-substrate metabolism. J. Nutr. Biochem. 17:571-588. crossref(new window)

43.
Kim, S. W. and R. A. Easter. 2003. Amino acid utilization for reproduction in sows. Page 203-222 in Amino Acids in Animal Nutrition. J. P. F. D'Mello, ed. CABI Publishing, Wallingford, UK.

44.
Kim, S. W., G. Wu and D. H. Baker. 2005. Amino acid nutrition of breeding sows during gestation and lactation. Pig News Info. CABI. 26:89N-99N.

45.
Kim, S. W., L. E. Hulbert, H. A. Rachuonyo and J. J. McGlone. 2004. Relative availability of iron in mined humic substances for weanling pigs. Asian-Aust. J. Anim. Sci. 17:1266-1270

46.
Kim, S. W., R. L. McPherson and G. Wu. 2004. Dietary arginine supplementation enhances the growth of milk-fed young piglets. J. Nutr. 134:625-630.

47.
Kim, S. W., R. D. Mateo, G. Wu, J. A. Carroll and I. Shinzato. 2006. Dietary L-arginine supplementation affects immune status of pregnant gilts. FASEB J. A266.1.

48.
Kim, S. W., R. L. McPherson and G. Wu. 2004a. Dietary arginine supplementation enhances the immune status of milk-fed young pigs. FASEB J. 18:A378.

49.
Kim, S. W., R. L. McPherson and G. Wu. 2004b. Dietary arginine supplementation enhances the growth of milk-fed young pigs. J. Nutr. 134:625-630.

50.
Kwon, H., T. E. Spencer, F. W. Bazer and G. Wu. 2003. Developmental changes of amino acids in ovine fetal fluids. Biol. Reprod. 68:1813-1820. crossref(new window)

51.
Maclennan, P. A., K. Smith, B. Weryk, P. W. Watt and M. J. Rennie. 1988. Inhibition of protein breakdown by glutamine in perfused rat skeletal muscle. FEBS Lett. 237:133-136. crossref(new window)

52.
Maclennan, P. A., R. A. Brown and M. J. Rennie. 1987. A positive relationship between protein synthetic rate and intracellular glutamine concentration in perfused rat skeletal muscle. FEBS Lett. 215:187-191. crossref(new window)

53.
Martin, P. M., A. E. Sutherland and L. J. Van Winkle. 2003. Amino acid transport regulates blastocyst implantation. Biol. Reprod. 69:1101-1108. crossref(new window)

54.
Mateo, R. D., G. Wu, J. A. Carroll, I. Shinzato and S. W. Kim. 2006. Dietary L-arginine supplementation improves pregnancy outcome in gilts. J. Anim. Sci. 84(Suppl. 2):7-8.

55.
Mattos, R., C. R. Staples and W. W. Thatcher. 2000. Effects of dietary fatty acids on reproduction in ruminants. Rev. Reprod. 5:38-45. crossref(new window)

56.
McCowen, K. C. and B. R. Bistrian. 2003. Immunonutrition: Problematic or problem solving? Am. J. Clin. Nutr. 77:764-770.

57.
McEntee W. J., and T. J. Cook. 1991. Serotonin, memory and the aging brain. Psychopharmacol. 103:143-149. crossref(new window)

58.
McPherson, R. L., F. Ji, G. Wu and S. W. Kim. 2004. Fetal growth and compositional changes of fetal tissues in the pigs. J. Anim. Sci. 82:2534-2540.

59.
Meijer, A. J. and P. F. Dubbelhuis. 2004. Amino acid signaling and the integration of metabolism. Biochem. Biophys. Res. Commun. 313:397-403. crossref(new window)

60.
Melchior, D., N. Le Floc'h and B. Seve. 2003. Effect of chronic lung inflammation on tryptophan metabolism in piglets. Adv. Exp. Med. Biol. 527:359-362.

61.
Muskiet, F. A. J., M. R. Fokkema, A. Schaafsma, E. R. Boersma, and M. A. Crawford. 2004. Is decahexaenoic acid (DHA) essential?: Lessons from DHA status regulation, our ancient diet, edpidemiology and randomized controlled trials. J. Nutr. 134:183-186.

62.
Newsholme, P., L. Brennnan, B. Rubi and P. Maechler. 2005. New insights into amino acid metabolism, beta-cell function and diabetes. Clin. Sci. 108:185-194. crossref(new window)

63.
Olsen, S. F., J. D. Sorensen, N. J. Secher, M. Hedegaard, T. B. Hentiksen, H. S. Hansen and A. Grant. 1992. Randomised controlled trial of effect of fish-oil supplementation on pregnancy duration. Lancet 25:1003-1007.

64.
Owens, S. and S. M. Innis. 1998. Docosahexaenoic and arachidonic acid prevent a decrease in dopaminergic and serotoninergic neurotransmitter in frontal cortex caused by a linoleic and $\alpha$-linolenic acid deficient diet in formula fed piglets. J. Nutr. 129:2088-2093.

65.
Parrybillings, M., J. Evans, P. C. Calder and E. A. Newsholme. 1990. Does glutamine contribute to immunosuppression after major burns? Lancet 336:523-525. crossref(new window)

66.
Platten, M., P. P. Ho, S. Youssef, P. Fontoura, H. Garren, E. M. Hur, R. Gupta, L. Y. Lee, B. A. Kidd, W. H. Robinson, R. A. Sobel, M. L. Selley and L. Steinman. 2005. Treatment of autoimmune neuroinflammation with a synthetic tryptophan metabolite. Sci. 310:850-855. crossref(new window)

67.
Ramsay, T. G., J. Karousis, M. E. White and C. K. Wolverton. 1991. Fatty acid metabolism by the porcine placenta. J. Anim. Sci. 69:3645-3654.

68.
Rice, R. 1999. Focus on omega-3. Ingred. Health Nutr. 2:11-15.

69.
Robinson, D. R., L. L. Xu, S. Tateno, M. Guo and R. B. Colvin. 1993. Suppression of autoimmune disease by dietary n-3 fatty acids. J. Lipid Res. 34:1435-1444.

70.
Rooke, J. A., A. G. Sinclair and M. Ewen. 2001a. Changes in piglet composition at birth in response to increasing maternal intake of long chain n-3 polyunsaturated fatty acids are nonlinear. Br. J. Nutr. 86:461-470. crossref(new window)

71.
Rooke, J. A., A. G. Sinclair and S. A. Edwards. 2001b. Feeding tuna oil to the sow at different times during pregnancy has different effects on piglet long-chain polyunsaturated fatty acid composition at birth and subsequent growth. Br. J. Nutr. 86:21- 30. crossref(new window)

72.
Rooke, J. A., A. G. Sinclair, S. A. Edwards, R. Cordoba, S. Pkiyachi, P. C. Penny, P. Penny, A. M. Finch and G. W. Horgan. 2001c. The effect of feeding salmon oil to sows throughout pregnancy on pre-weaning mortality of piglets. Anim. Sci. 73:489-500.

73.
Rooke, J. A., I. M. Bland and S. A. Edwards. 1998. Effect of feeding tuna oil or soybean oil as supplements to sow in late pregnancy on piglet tissue composition and viability. Br. J. Nutr. 80:273-280. crossref(new window)

74.
Rooke, J. A., M. Shanks and S. A. Edwards. 2000. Effect of offering maize, linseed or tuna oils throughout pregnancy and lactation on sow and piglet tissue composition and piglet performance. Anim. Sci. 71:289-299.

75.
Sastry, P. S. 1985. Lipids of nervous tissue: composition and metabolism. Prog. Lipid Res. 24:69-176. crossref(new window)

76.
Self, J. T., T. E. Spencer, G. A. Johnson, J. Hu, F. W. Bazer and G. Wu. 2004. Glutamine synthesis in the developing porcine placenta. Biol. Reprod. 70:1444-1451. crossref(new window)

77.
Shi, W., C. J. Meininger, T. E. Haynes, K. Hatakeyama and G. Wu. 2004. Regulation of tetrahydrobiopterin synthesis and bioavailability in endothelial cells. Cell Biochem. Biophys. 41:415-433. crossref(new window)

78.
Simopoulos, A. P. 1991. Omega-3 fatty acids in health and disease and in growth and development. Am. J. Clin. Nutr. 54:438-463.

79.
Spencer, J. D., L. Wilson, S. K. Webel, R. L. Moser and D. M. Webel. 2004. Effect of feeding protected n-3 polyunsaturated fatty acids ($Fertilium^{TM}$) on litter size in gilts. J. Anim. Sci. 82 (Suppl. 1):211(Abstr.).

80.
Stead, L. M., J. T. Brosnan, M. E. Brosnan, D. E. Vance and R. L. Jacobs. 2006. Is it time to reevaluate methyl balance in humans? Am. J. Clin. Nutr. 83:5-10.

81.
Taugbol, O., T. Framstad and K. Saarem. 1993. Supplements of cod liver oil to lactating sows. Influence on milk fatty acid composition and growth performance of piglets. Zentralbl Veterinarmed A. 40:437-443.

82.
Thulin, A. J., G. L. Allee, D. L. Harmon and D. L. Davis. 1989. Utero-placental transfer of octanoic, palmitic and linoleic acids during late gestation in gilts. J. Anim. Sci. 67:738-745.

83.
Tischler, M. E., M. Desautels and A. L. Goldberg. 1982. Does leucine, leucyl-transfer RNA, or some metabolite of leucine regulate protein synthesis and degradation in skeletal and cardiac muscle? J. Biol. Chem. 257:1613-1621.

84.
Trottier, N. L. and L. J. Johnston. 2001. Feeding gilts during development and sows during gestation and lactation. Page 725-769 in Swine Nutrition. 2nd (Ed. A. J. Lewis and L. L. Southern), CRC Press, New York.

85.
Trottier, N. L., C. F. Shipley and R. A. Easter. 1997. Plasma amino acid uptake by the mammary gland of the lactating sow. J. Anim. Sci. 75:1266-1278.

86.
Turek, J. J., I. A. Schoenlein, B. A. Watkins, W. G. Van Alstine, L. K. Clark and K. Knox. 1996. Dietary polyunsaturated fatty acids modulate responses of pigs to mycoplasma hyopneumonia infection. J. Nutr. 126:1541-1548.

87.
Uauy, R. and C. Castillo. 2003. Lipid requirement of infants: Implications for nutrient composition of fortified complementary foods. J. Nutr. 133:2962S-2972S.

88.
Watford, M. and G. Wu. 2005. Glutamine metabolism in uricotelic species: variation in skeletal muscle glutamine synthetase, glutaminase, glutamine levels and rates of protein synthesis. Comp. Biochem. Physiol. B. 140:607-614. crossref(new window)

89.
Webel, S. K., E. R. Otto-Tice, R. L. Moster and D. E. Orr, Jr. 2004. Effect of feeding duration of protected n-3 polyunsaturated fatty acid ($Fertilium^{TM}$) on litter size and embryo survival in sows. J. Anim. Sci. 82(Suppl. 1):212(Abstr.).

90.
Webel, S. K., E. R. Otto, D. M. Webel, R. L. Moser, J. D. Spencer and D. E. Orr. 2003. Effect of protected n-3 polyunsaturated fatty acids ($Fertilium^{TM}$) on litter size in sows. J. Anim. Sci. 81 (Suppl. 1):18 (Abstr.).

91.
Wu, G. 1996. Effects of concanavalin A and phorbol myristate acetate on glutamine metabolism and proliferation of porcine intraepithelial lymphocytes. Comp. Biochem. Physiol. 114A:363-368.

92.
Wu, G. 1997. Synthesis of citrulline and arginine from proline in enterocytes of postnatal pigs. Am. J. Physiol. 272:G1382-G1390.

93.
Wu, G. and C. J. Meininger. 2002. Regulation of nitric oxide synthesis by dietary factors. Annu. Rev. Nutr. 22:61-86. crossref(new window)

94.
Wu, G. and D. A. Knabe. 1994. Free and protein-bound amino acids in sow's colostrums and milk. J. Nutr. 124:415-424.

95.
Wu, G. and J. R. Thompson. 1990. The effect of glutamine on protein turnover in chick skeletal muscle in vitro. Biochem. J. 265:593-598.

96.
Wu, G. and J. T. Self. 2005. Amino acids: metabolism and functions. In: Encyclopedia of Animal Science (Ed. W. G. Pond and A. W. Bell). Marcel Dekker, Inc., New York, pp. 9-12.

97.
Wu, G. and S. M. Morris, Jr. 1998. Arginine metabolism: nitric oxide and beyond. Biochem. J. 336:1-17.

98.
Wu, G., C. J. Field and E. B. Marliss. 1991. Glutamine and glucose metabolism in rat splenocytes and mesenteric lymph node lymphocytes. Am. J. Physiol. 260:E141-E147.

99.
Wu, G., D. A. Knabe and S. W. Kim. 2004c. Arginine nutrition in neonatal pigs. J. Nutr. 134:2783S-2390S.