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Antioxidant Favors the Developmental Competence of Porcine Parthenogenotes by Reducing Reactive Oxygen Species
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 Title & Authors
Antioxidant Favors the Developmental Competence of Porcine Parthenogenotes by Reducing Reactive Oxygen Species
Hossein, Mohammad Shamim; Kim, Yeun Wook; Park, Seon Mi; Koo, Ok Jae; Hashem, Md Abul; Bhandari, Dilip P; Jeong, Yeon Woo; Kim, Sue; Kim, Ji Hye; Lee, Eu Gine; Park, Sun Woo; Kang, Sung Keun; Lee, Byeong Chun; Hwang, Woo Suk;
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Reactive oxygen species (ROS) generate during electrical activation of oocytes which has detrimental effects on embryo survival when overwhelmed. The present study was designed to investigate the ability of L-ascorbic acid, a novel water soluble antioxidant, to reduce the ROS level in developing embryos and their subsequent effects on embryo development in vitro. The compact cumulus oocyte complexes (COCs) were cultured in tissue culture medium (TCM)-199 supplemented with 10 ng/ml epidermal growth factor, 4 IU/ml pregnant mare serum gonadotropin (PMSG), and human chorionic gonadotropin (hCG) and 10% (v/v) porcine follicular fluid (pFF) for 44 h. After maturation culture, the denuded oocytes were activated with a single DC pulse of 2.0 kV/cm in 0.3 M mannitol solution containing 0.5 mM of HEPES, 0.1 mM of and 0.1 mM of for using a BTX Electro-cell Manipulator. The activated oocytes were cultured in modified North Carolina State University-23 (mNSCU-23) medium for 168 h. The level of in each embryo was measured by the dichlorohydrofluorescein diacetate (DCHFDA) method at 48 h after activation. The blastocyst formation rate was significantly higher when culture medium was supplemented with 50 and L-ascorbic acid (31.2 and 38.7%, respectively) compared to non-supplemented (16.1%) group. Accordingly, significantly more cells in blastocyst were found for 50 and L-ascorbic acid (50.0 and 56.4, respectively) compared to 0 and L-ascorbic acid (36.5 and 39.8, respectively). L-ascorbic acid reduces the level in developing embryos in a dose-dependant manner. The level (pixels/ embryos) was 191.5, 141.0, 124.0 and 163.3 for 0, 50, 100 and L-ascorbic acid, respectively. So, we recommend to supplement 50 or L-ascorbic acid in porcine in vitro culture medium.
Antioxidant;Parthenogenitic Activation;Porcine;Embryo;Reactive Oxygen Species;
 Cited by
Parthenogenetic Activation of Porcine Oocytes and Isolation of Embryonic Stem Cells-like Derived from Parthenogenetic Blastocysts,;;;;;;

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