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Nitrogen-15 Determination in Tissues of Laying Hens Fed on Different Levels of Chloride () Diets
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 Title & Authors
Nitrogen-15 Determination in Tissues of Laying Hens Fed on Different Levels of Chloride () Diets
Nurhayati, Nurhayati; Thinggaard, Grete; Chakeredza, S.; Reineking, A.; Langel, R.; ter Meulen, U.;
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An experiment was conducted to determine the distribution of nitrogen-15 in tissues of laying hens receiving different levels of -CCC in diets. Twenty brown laying hens were divided into four groups and randomly assigned into one of four dietary treatment groups consisting of 0, 5, 50 and 100 ppm -CCC inclusion. The hens were individually fed with the -CCC diets in battery cages for 11 days and then all hens restored to feeding on the control diet for 7 days. After eleven days, eight hens were slaughtered, and the others were slaughtered seven days after -CCC diets withdrawal. Samples of blood, liver, heart and meat were collected and their contents were determined. The excess (-ex) and atom percentage excess in were calculated. The -ex and atom percentage excess increased significantly (p<0.05) with increasing levels of -CCC in diets in all tissues after feeding -CCC diets for eleven days. The highest concentration of -ex and atom percentage excess were detected in blood, followed in order by liver, heart and thigh meat. The concentrations reduced significantly (p<0.05) after -CCC diets were withdrawn. Comparison between treatment groups showed that -ex and atom percentage excess were still higher in hens that had been fed diets with higher levels of -CCC. This study showed that nitrogen-15 was distributed in blood, liver, heart and meat of laying hens.
Atom Percentage Excess Nitrogen-15;Delta Nitrogen-15 Excess;Laying Hen Tissues;Nitrogen-15;
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