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Insertional Variations of Two Porcine Endogenous Retroviruses (PERVs) in Korean Native Pigs and Asian Wild Boars
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 Title & Authors
Insertional Variations of Two Porcine Endogenous Retroviruses (PERVs) in Korean Native Pigs and Asian Wild Boars
Jung, K.C.; Yu, S.L.; Kim, T.H.; Jeon, J.T.; Rogel-Gaillard, C.; Park, C.S.; Jin, D.I.; Moran, C.; Lee, J.H.;
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Porcine Endogenous Retroviruses (PERVs) are a major concern in relation to xenotransplantation. Previous research indicated that PERVs are present at about 50 copies in the pig genome and their chromosomal insertion sites are different among pig breeds. We examined nine Korean native pigs and seven Asian Wild Boars for the presence of a PERV-A at SSC 1q2.4 and a PERV-B at SSC 7p1.1-2 previously reported in a Large White pig. The PERV-B at locus 7p1.1-2 displayed insertional variability in Korean native pigs and Asian Wild Boars. Using the primers for the PERV-A at 1q2.4 from Large White pig, we only can amplify an unclassified 798 bp sequence, which showed insertional variability only in Korean native pigs. This study indicates that there are differences within and between Asian and European pigs in PERV insertions and suggests that selection could generate PERV-free lines of pigs more suitable for xenotransplantation.
Pigs;Insertional Variations;Porcine Endogenous Retroviruses (PERVs);Xenotransplantation;
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Asian-Australasian Journal of Animal Sciences, 2008. vol.21. 11, pp.1572-1575 crossref(new window)
Akiyoshi, D. E., M. Denaro, H. Zhu, J. L. Greenstein, P. Banerjee and J. A. Fishman. 1998. Identification of a full-length cDNA for an endogenous retrovirus of miniature swine. J. Virol. 72:4503-4507.

Chari, R. S., B. H. Collins, J. C. Magee and 6 other authors. 1994. Brief report: treatment of hepatic failure with ex vivo pig-liver perfusion followed by liver transplantation. N. Engl. J. Med. 331:234-237. crossref(new window)

Czauderna, F., N. Fisher, K. Boller, R. Kurth and R. R. Tonjes. 2000. Establishment and characterization of molecular clones of porcine endogenous retroviruses replicating on human cells. J. Virol. 74:4028-4038. crossref(new window)

Deacon, T., J. Schumacher, J. Dinsmore, C. Thomas, P. Palmer, S. Kott, A. Edge, D. Penney, S. Kassissieh, P. Dempsey and O. Isacson. 1997. Histological evidence of fetal pig neural cell survival after transplantation into a patient with Parkinson's disease. Nat. Med. 3:350-353. crossref(new window)

Deng, Y. -M., J. H. Lee, C. Moran, B. Tuch and W. D. Rawlinson. 2000. Mapping dispersed repetitive loci using semi-specific PCR cloning and somatic cell hybrid mapping. Nucleic Acids Res. 28:E108. crossref(new window)

Gorbovitskaia, M., Z. Liu, N. Bourgeaux, N. Li, Z. Lian, P. Chardon and C. Rogel-Gaillard. 2003. Characterization of two porcine endogenous retrovirus integration loci and variability in pigs. Immunogenetics. 55:262-270. crossref(new window)

Groth, C. G., O. Korsgren, A. Tibell, J. Tollemar, E. Moller, J. Bolinder, J. Ostman, F. P. Reinholt, C. Hellerstrom and A. Andersson. 1994. Transplantation of porcine fetal pancreas to diabetic patients. Lancet. 344:1402-1404. crossref(new window)

Jeon, J. T., E. W. Park, H. J. Jeon, T. H. Kim, K. T. Lee and I. C. Cheong. 2003. A large-insert porcine library with sevenfold genome coverage: a tool for positional cloning of candidate genes for major quantitative traits. Mol. Cells. 16:113-116.

Le Tissier, P., J. P. Stoye, Y. Takeuchi, C. Patience and R. A. Weiss. 1997. Two sets of human-tropic pig retrovirus. Nature. 389:681-682. crossref(new window)

Lee, J. H. and C. Moran. 2001. Current status of xenotransplantation -A review. Asian-Aust. J. Anim. Sci. 14(10):1497-1504.

Lee, J. H., G. C. Webb, R. D. M. Allen and C. Moran. 2002. in Westran pigs. J. Virol. 76:5548-5556. crossref(new window)

Martin, U., V. Kiessig, J. H. Blusch, A. Haverich, K. von der Helm, T. Herden and G. Steinhoff. 1998a. Expression of pig endogenous retrovirus by primary porcine endothelial cells and infection of human cells. Lancet. 352:692-694. crossref(new window)

Martin, U., G. Steinhoff, V. Kiessig, M. Chikobava, M. Anssar, T. Morschheuser, B. Lapin and A. Haverich. 1998b. Porcine endogenous retrovirus (PERV) was not transmitted from transplanted porcine endothelial cells to baboons in vivo. Transpl. Int. 11:247-251. crossref(new window)

Niebert, M., C. Rogel-Gaillard, P. Chardon and R. R. Tonjes. 2002. Characterization of chromosomally assigned replicationcompetent gamma porcine endogenous retroviruses derived from a Large White pig and expression in human cells. J. Virol. 76:2714-2720. crossref(new window)

Paradis, K., G. Langford, Z. Long, W. Heneine, P. Sandstrom, W. M. Switzer, L. E. Chapman, C. Lockey, D. Onions and E. Otto. 1999. Search for cross-species transmission of porcine endogenous retrovirus in patients treated with living pig tissue. Sci. 285:1236-1241. crossref(new window)

Patience, C., Y. Takeuchi and R. A. Weiss. 1997. Infection of human cells by an endogenous retrovirus of pigs. Nat. Med. 3:282-286. crossref(new window)

Patience, C., G. S. Patton, Y. Takeuchi, R. A. Weiss, M. O. McClure, L. Rydberg and M. E. Breimer. 1998. No evidence of pig DNA or retroviral infection in patients with short-term extracorporeal connection to pig kidneys. Lancet. 352:699-701. crossref(new window)

Pitkin, Z. and C. Mullon. 1999. Evidence of absence of porcine endogenous retrovirus (PERV) infection in patients treated with a bioartificial liver support system. Artif. Organs. 23:829-833. crossref(new window)

Rogel-Gaillard, C., N. Bourgeaux, A. Billault, M. Vaiman and P. Chardon. 1999. Construction of a swine BAC library: application to the characterization and mapping of porcine type C endoviral elements. Cytogenet. Cell Genet. 85:205-211. crossref(new window)

Rogel-Gaillard, C., H. Hayes, N. Bourgeaux and P. Chardon. 2001. Assignment of two new loci for gamma 1 porcine endogenous retroviruses (gamma 1 PERV) to pig chromosome bands 2q21 and 11q12 by in situ hybridization. Cytogenet. Cell Genet. 95:112-113. crossref(new window)

Switzer, W. M., V. Shanmugam, L. Chapman and W. Heneine. 1999. Polymerase chain reaction assays for the diagnosis of infection with the porcine endogenous retrovirus and the detection of pig cells in human and nonhuman recipients of pig xenografts. Transplantation. 68:183-188. crossref(new window)

van der Laan, L. J., C. Lockey, B. C. Griffeth and 8 other authors. 2000. Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice. Nature. 407:90-94. crossref(new window)

Wilson, C. A., S. Wong, J. Muller, C. E. Davidson, T. M. Rose and P. Burd. 1998. Type C retrovirus released from porcine primary peripheral blood mononuclear cells infects human cells. J. Virol. 72:3082-3087.

Yerle, M., P. Pinton, A. Robic, A. Alfonso, Y. Palvadeau, C. Delcros, R. Hawken, L. Alexander, C. Beattie, L. Schook, D. Milan and J. Gellin. 1998. Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet Cell Genet. 82(3-4):182-188. crossref(new window)