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Identification of a Novel SNP Associated with Meat Quality in C/EBP Gene of Korean Cattle
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 Title & Authors
Identification of a Novel SNP Associated with Meat Quality in C/EBP Gene of Korean Cattle
Shin, S.C.; Kang, M.J.; Chung, E.R.;
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CCAAT/enhancer binding protein () plays an important role in lipid deposition and adipocyte differentiation. In order to find genetic markers to improve the meat quality of Korean cattle, the bovine gene was chosen as a candidate gene to investigate its association with carcass and meat quality traits in Korean cattle. A single nucleotide polymorphism (SNP) was identified at position 271 (A/C substitution) of coding region in the gene. A PCR-RFLP procedure with restriction enzyme SmaI was developed for determining the marker genotypes. The frequencies of alleles C and A and were 0.374 and 0.626, respectively. The genotype frequencies for CC, AC and AA were 12.9, 49.0 and 38.1%, respectively, in Korean cattle population. The frequencies of genotype were in agreement with Hardy-Weinberg equilibrium. Association analysis indicated that the gene-specific SNP marker of showed a significant association with marbling score (p<0.05). The animals with AA genotype had higher marbling score than those with the AC or CC genotype. Although further studies are needed to validate our results, the gene could be useful as a genetic marker for carcass and meat quality traits in Korean cattle.
Gene;SNP Marker;Meat Quality, Korean Cattle;
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아세아태평양축산학회지, 2007. vol.20. 11, pp.1662-1669 crossref(new window)
Association between polymorphisms of Myf5 and POU1F1 genes with growth and carcass traits in Hanwoo (Korean cattle),;;;;;;;;;

Genes and Genomics, 2011. vol.33. 4, pp.425-430 crossref(new window)
gene appears to be associated with intramuscular fat deposition in longissimus muscle in Australian cattle, Animal Genetics, 2009, 40, 5, 770  crossref(new windwow)
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Casas, E., J. W. Keele, S. D. Shackelford, M. Koohmaraie, T. S. Sonstegard, T. P. Smith, S. M. Kappes and R. T. Stone. 1998. Association of the muscle hypertrophy locus with carcass traits in beef cattle. J. Anim. Sci. 76:468-473. crossref(new window)

Casas, E., R. T. Stone, J. W. Keele, S. D. Shackelford, S. M. Kappes and M. Koohmaraie. 2001. A comprehensive search for quantitative traits loci affecting growth and carcass composition of cattle segregating alternative forms of myostatin. J. Anim. Sci. 79:854-860. crossref(new window)

Shin, S. C. and E. R. Chung. 2007a. Association of SNP marker in the leptin gene with carcass and meat quality traits in Korean cattle. Asian-Aust. J. Anim. Sci. 20:1-6.

Shin, S. C. and E. R. Chung. 2007b. Association of SNP marker in the thyroglobulin gene with carcass and meat quality traits in Korean cattle. Asian-Aust. J. Anim. Sci. 20:172-177.

Dekkers, J. C. M., M. F. Rothschild and M. M. Malek. 2001. Potential and application of marker assisted selection for meat quality. Second International Virtual Conference on Pork Quality. 240-263.

Fajas, L., J. C. Fruchart and J. Auwerx. 1998. Transcriptional control of adipogenesis. Curr. Opin. Cell Biol. 10:165-173. crossref(new window)

Falconer, D. S. and T. F. C. Mackay. 1996. Introduction to Quantitative Genetics. 4th ed. Addison Wesley Limited, Edinburg Gate, Harlow Essex, UK.

Gregoire, F. M., C. M. Smas and H. S. Sul. 1998. Understanding adipocyte differentiation. Physiol. Rev. 78:783-809. crossref(new window)

Hovenier, R., E. Kanis, T. Van Asseldink and N. G. Westerink. 1993. Breeding for pig meat quality in halothane negative populations-a review. Pig News Info. 14:17N-35N.

Ihara, N., H. Yamakuchi, T. Hirano, H. Takeda, Y. Taniguchi, Y. Sasaki, S. K. Davis, J. F. Taylor, W. Barendse, Y. Sugimoto. 1998. Physical and genetic mapping of bovine CEBP$\alpha$ and PPAR$\gamma$ genes. Anim. Genet. 29:398-400. crossref(new window)

Ihara, N., H. Yamakuchi, Y. Taniguchi, Y. Sasaki, G. L. Bennett, S. Kappes and Y. Sugimoto. 2003. Mapping of bovine CEBPD to BTA14q15-17. Anim. Genet. 34:465-476 crossref(new window)

Jeoung, Y. H., S. M. Lee, H. Y. Park, D. H. Yoon, S. J. Moon, E. R. Chung and M. J. Kang. 2004. Molecular cloning and mRNA expression of the Hanwoo CCAAT/enhancing-binding Protein $\alpha$(C/EBP$\alpha$) gene. J. Anim. Sci. Technol. (Kor.). 46(6):909-916. crossref(new window)

Macneil, M. D. and M. D. Grosz. 2002. Genome-wide scans for QTR affecting carcass traits in Hereford x composite double backcross populations. J. Anim. Sci. 80:2316-2324.

Meuwissen, T. H. E. and M. E. Goddard. 1996. The use of marker haplotypes in animal breeding schemes. Genet. Sel. Evol. 28(2):161-176. crossref(new window)

Miller, S. A., D. D. Kykes and H. F. Polesky. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16:1215. crossref(new window)

Morrison, R. F. and S. R. Farmer. 1999. Insight into the transcriptional control of adipocyte differentiation. J. Cell Biochem. 32-33 (Suppl.). 59-67.

Polineni, P., P. Aragonda, S. R. Xavier, R. Furuta and D. L. Adelson. 2006. The bovine QTL viewer: a web accessible database of bovine quantitative trait loci. BMC Bioinfomatics 7:283. crossref(new window)

Rosen, E. D., C. J. Walkey, P. Puiserver and B. M. Spiegelman. 2000. Transcriptional regulation of adipogenesis. Genes Dev. 14:1293-1307.

Rothschild, M. F. and G. S. Plastow. 1999. Advances in pig genomes and industry applications. AgBiotechNet 1, 1-8.

Salma, N., H. Xiao and A. N. Imbalzano. 2006. Temporal recruitment of CCAAT/enhancer-binding proteins to early and late adipogenic promoters in vivo. J. Mol. Endocrinol. 36:139-161. crossref(new window)

Taniguchi, Y. and Y. Sasaki. 1996. Rapid communication: Nucleotide sequence of bovine C/EBP$\alpha$ gene. J. Anim. Sci. 74:2554. crossref(new window)

Wheeler, T. L., L. V. Cundiff and R. M. Koch. 1994. Effect of marbling degree on beef palatability in Bos indicus cattle. J. Anim. Sci. 72:3145-3151. crossref(new window)

Yamamoto, H., S. Kurebayashi, T. Hirose, H. Kouhara and S. Kasayama. 2002. Reduced IRS-2 and GLUT4 expression in PPAR${\gamma}2$-induced adipocytes derived from C/EBP$\beta$ and C/EBP$\gamma$-deficient mouse embryonic fibroblasts. J. Cell Sci. 115:3601-3607 crossref(new window)