Advanced SearchSearch Tips
Steroid Effects on Cell Proliferation, Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Steroid Effects on Cell Proliferation, Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells
Lee, Eun Ju; Choi, Jinho; Hyun, Jin Hee; Cho, Kyung-Hyun; Hwang, Inho; Lee, Hyun-Jeong; Chang, Jongsoo; Choi, Inho;
  PDF(new window)
The present study was conducted to establish primary bovine muscle satellite cell (MSC) culture conditions and to investigate the effects of various steroid hormones on transcription of the genes involved in muscle cell proliferation and differentiation. Of three different types of proteases (type II collagenase, pronase and trypsin-EDTA) used to hydrolyze the myogenic satellite cells from muscle tissues, trypsin-EDTA treatment yielded the highest number of cells. The cells separated by hydrolysis with type II collagenase and incubated on gelatin-coated plates showed an enhanced cell attachment onto the culture plate and cell proliferation at an initial stage of cell growth. In this study, the bovine MSCs were maintained in vitro up to passage 16 without revealing any significant morphological change, and even to when the cells died at passage 21 with decreased or almost no cell growth or deformities. When the cells were incubated in a steroid-depleted environment (DMEM(-)/10% CDFBS (charcoal-dextran stripped FBS)), they grew slowly initially, and were widened and deformed. In addition, when the cells were transferred to an incubation medium containing steroid (DMEM(+)/10% FBS), the deformed cells resumed their growth and returned to a normal morphology, suggesting that steroid hormones are crucial in maintaining normal MSC morphology and growth. The results demonstrated that treatments with 19-nortestosterone and testosterone significantly increased AR gene expression (p<0.05), implying that both testosterone and 19-nortestosterone bind with AR and that the hormone bound-AR complex up-regulates the genes of its own receptor (AR) plus other genes involved in satellite cell growth and differentiation in bovine muscle.
Bovine Muscle Satellite Cell;Steroid;Primary Cell Culture;
 Cited by
Testicular Expression of Steroidogenic Enzyme Genes Is Related to a Transient Increase in Serum 19-nortestosterone during Neonatal Development in Pigs,;;;;;;;;;;

아세아태평양축산학회지, 2007. vol.20. 12, pp.1832-1842 crossref(new window)
Differential Proteome Expression of In vitro Proliferating Bovine Satellite Cells from Longissimus Dorsi, Deep Pectoral and Semitendinosus Muscle Depots in Response to Hormone Deprivation and Addition,;;;;;;;;;;

Journal of Animal Science and Technology, 2009. vol.51. 6, pp.459-470 crossref(new window)
돼지 고기의 아나볼릭 스테로이드가 Myogenic Satellite Cell의 증식과 분화에 미치는 영향,이동목;이기호;전용필;전태훈;최인호;

한국축산식품학회지, 2010. vol.30. 5, pp.842-850 crossref(new window)
한우(Bos taurus coreanae) 유래 myoblast에서 전사인자 과발현에 의한 지방세포로의 교차 분화 유도,문양수;

생명과학회지, 2012. vol.22. 10, pp.1316-1323 crossref(new window)
Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation, In Vitro Cellular & Developmental Biology - Animal, 2011, 47, 7, 438  crossref(new windwow)
Effects of Ectopic Expression of Transcription Factors on Adipogenic Transdifferentiation in Bovine Myoblasts, Journal of Life Science, 2012, 22, 10, 1316  crossref(new windwow)
Effect of Gender-Specific Adult Bovine Serum on Gene Expression During Myogenesis, Journal of Animal Science and Technology, 2012, 54, 3, 219  crossref(new windwow)
Meeting the meat: delineating the molecular machinery of muscle development, Journal of Animal Science and Technology, 2016, 58, 1  crossref(new windwow)
Allen, R. E. and L. L. Rankin. 1990. Regulation of satellite cell during skeletal muscle growth and development. PSEMB. 194:81-86.

Allen, R. E., R. A. Merkel and R. B. Young. 1979. Cellular aspects of muscle growth: Myogenic cell proliferation. J. Anim. Sci. 49:115-127. crossref(new window)

Apple, J. K., M. E. Dikeman, D. D. Simms and G. Kuhl. 1991. Effects of synthetic hormone implants, singularly or in combinations, on performance, carcass traits, and longissimus muscle palatability of Holstein steers. J. Anim. Sci. 69:4437-4448. crossref(new window)

Bischoff, R. 1974. Enzymatic liberation of myogenic cells from adult rat muscle. Anat. Rec. 180:645-662. crossref(new window)

Bonavaud, S., O. Agbulut, G. D'Honneur, R. Nizard, V. Mouly and G. Butler-Browne. 2002. Preparation of isolated human muscle fibers: a technical report. In vitro Cell. Dev. Biol. Anim. 38:66-72. crossref(new window)

Campion, D. R. 1984. The muscle satellite cell: Int. Rev. Cytol. 87:225-251. crossref(new window)

Chen, J. C. J. and D. J. Goldhamer. 2003. Skeletal muscle stem cells. Reproductive Biol. Endocrinol. 1:101-107. crossref(new window)

Choi, I., L. J. Gudas and B. S. Katzenellenbogen. 2000. Regulation of keratin 19 gene expression by estrogen in human breast cancer cells and identification of the estrogen responsive gene region. Mol. Cell Endocrinol. 164:225-237. crossref(new window)

Cornelison, D. D. and B. J. Wold. 1997. Single-cell analysis of regulatory gene expression in quiescent and activated mouse skeletal muscle satellite cells. Dev. Biol. 191:270-283. crossref(new window)

Dodson, M. V., B. A. Mathison and B. D. Mathison. 1990. Effects of medium and substratum on ovine satellite cell attachment, proliferation and differentiation in vitro. Cell. Diff. Dev. 29:59-66. crossref(new window)

Dodson, M. V., D. C. McFarland, A. L. Grant, M. E. Doumit and S. G. Velleman. 1996. Extrinsic regulation of domestic animalderived satellite cells. Dom. Anim. Endocrin. 13:107-126. crossref(new window)

Dodson, M. V., E. L. Martin, M. A. Brannon, B. A. Mathison and D. C. McFarland. 1987. Optimization of bovine satellite cellderived myotube formation in vitro. Tissue and Cell. 19:159-166. crossref(new window)

Dodson, M. V., D. C. McFarland, E. L. Martin and M. A. Brannon. 1986. Isolation of satellite cells from ovine skeletal muscle. J. Tiss. Cul. Meth. 10:233-237. crossref(new window)

Doumit, M. E. and R. A. Merkel. 1992. Conditions for the isolation and culture of porcine myogenic satellite cells. Tissue and Cell. 24:253-262. crossref(new window)

Doumit, M. E., D. R. Cook and R. A. Merkel. 1996. Testosterone up-regulates androgen receptors and decreases differentiation of porcine myogenic satellite cells in vitro. Endocrinol. 137:1385-94. crossref(new window)

Duclos, M. J., B. Chevalier, C. Goddard and J. Simon. 1993. Regulation of amino acid transport and protein metabolism in myotubes derived from chicken muscle satellite cells by IGF-I. J. Cell. Physiol. 157:650-657. crossref(new window)

Greene, E. A. and R. H. Raub. 1992. Procedures for harvesting satellite cells from equine skeletal muscle. Equine. Nut. Phys. Soc. 12:33-35.

Herschler, R. C., A. W. Olmsted, A. J. Edwards, R. L. Hale, T. Montgomery, R. L. Preston, S. J. Bartle and J. J. Sheldon. 1995. Production responses to various doses and ratios of estradiol benzoate and trenbolone acetate implants in steers and heifers. J. Anim. Sci. 73:2873-2881. crossref(new window)

Hunt, D. W., D. M. Henricks, G. C. Skelley and L. M. Grimes. 1991. Use of trenbolone acetate and estradiol in intact and castrate male cattle: Effects on growth, serum hormones, and carcass characteristics. J. Anim. Sci. 69:2452-2462. crossref(new window)

Johnson, B. J., P. T. Anderson, J. C. Meiske and W. R. Dayton. 1996. Effect of a combined trenbolone and estradiol implant on steroid hormone levels, feedlot performance, carcass characteristics and carcass composition of feedlot steers. J. Anim. Sci. 74:363-371. crossref(new window)

Johnson, B. J., N. Halstead, M. E. White, M. R. Hathaway and W. R. Dayton. 1998. Activation state of muscle satellite cells isolated from steers implanted with a combined trenbolone acetate and estradiol implant. J. Anim. Sci. 76:2779-2786. crossref(new window)

Kahlert, S., C. Grohe, R. H. Karas, K. Lobbert, L. Neyses and H. Vetter. 1997. Effects of estrogen on skeletal myoblast growth. Biochem. Biophys. Res. Commun. 232:373-378. crossref(new window)

Kamanga-Sollo, E., M. S. Pampusch, G. Xi, M. E. White, M. R. Hathaway and W. R. Dayton. 2004. IGF-I mRNA levels in bovine satellite cell cultures: effects of fusion and anabolic steroid treatment. J. Cell Physiol. 201:181-189. crossref(new window)

Katzenellenbogen, B. S., I. Choi, R. Delage-Mourroux, T. R. Ediger, P. G. Martini, M. Montano, J. Sun, K. Weis and J. A. Katzenellenbogen. 2000. Molecular mechanisms of estrogen action: selective ligands and receptor pharmacology. J. Steroid Biochem. Mol. Biol. 74:279-285. crossref(new window)

Lay, S. L., Lefrere, C. Trautwein, I. Dugail and S. Krief. 2002. Insulin and sterol-regulatory element-binding protein-1c (SREBP-1C) regulation of gene expression in 3T3-L1 adipocytes. J. Biol. Chem. 277:35625-35634. crossref(new window)

Lindquist, D. L. and P. A. de Alarcon. 1987. Charcoal-dextran treatment of fetal bovine serum removes an inhibitor of human CFU-megakaryocytes. Exp. Hematol. 15:234-238.

McFarland, D. C., M. E. Doumit and R. D. Minshell. 1988. The turkey myogenic satellite cell: Optimization of in vitro proliferation and differentiation. Tissue and Cell. 20:899-908. crossref(new window)

Oh, Y. S., S. B. Cho, K. H. Beak and C. B. Choi. 2005. Effects of Testosterone, $17{\beta}$-estradiol, and Progesterone on the Differentiation of Bovine Intramuscular Adipocytes. Asian-Aust. J. Anim. Sci. 18:1589-1593. crossref(new window)

Seale, P., L. A. Sabourin, A. Girgis-Gabardo, P. Gruss and M. A. Rudnicki. 2000. Pax7 is required for the specification of myogenic satellite cells. Cell. 102:777-786. crossref(new window)

Shen, H. C. and G. A. Coetzee. 2005. The androgen receptor: unlocking the secrets of its unique transactivation domain. Vitam Horm. 71:301-319. crossref(new window)

Sinha-Hikim, I., W. E. Taylor, N. F. Gonzalez-Cadavid, W. Zheng and S. Bhasin. 2004. Androgen receptor in human skeletal muscle and cultured muscle satellite cells: up-regulation by androgen treatment. J. Clin. Endocrinol. Metab. 89:5245-5255. crossref(new window)

Thompson, S. H., L. K. Boxhorn, W. Y. Kong and R. E. Allen. 1989. Trenbolone alters the responsiveness of skeletal muscle satellite cells to fibroblast growth factor and insulin-like growth factor I. Endocrinol. 124:2110-2117. crossref(new window)

Tricarico, C., P. Pinzani, S. Bianchi, M. Paglierani, V. Distante, M. Pazzagli, S. A. Bustin and C. Orlando. 2002. Quantitative realtime reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal. Biochem. 309:293-300. crossref(new window)