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Effects of Heart Fatty Acid-binding Protein Genotype on Intramuscular Fat Content in Duroc Pigs Selected for Meat Production and Meat Quality Traits
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 Title & Authors
Effects of Heart Fatty Acid-binding Protein Genotype on Intramuscular Fat Content in Duroc Pigs Selected for Meat Production and Meat Quality Traits
Uemoto, Yoshinobu; Suzuki, Keiichi; Kobayashi, Eiji; Sato, Syushi; Shibata, Tomoya; Kadowaki, Hiroshi; Nishida, Akira;
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 Abstract
Using multi-trait animal model BLUP, selection was conducted over seven generations for growth rate (DG), real-time ultrasound loin-eye muscle area (LEA), backfat thickness (BF), and intramuscular fat content (IMF) to develop a new line of purebred Duroc pigs with enhanced meat production and meat quality. This study was intended to investigate the relationship between restriction fragment length polymorphism (RFLP) of a heart fatty acid-binding protein (H-FABP) gene and intramuscular fat content (IMF) of this Duroc purebred population. The present experiment examined the RFLP of 499 slaughtered pigs. The DNA was separated from the blood or ear tissue of the pigs, which were slaughtered at 105 kg of body weight. Intramuscular fat content of the longissimus muscle was measured using chemical analysis. A significant difference was detected in the breeding value of IMF among the H-FABP PCR RFLP genotypes. The AA genotype has a significantly larger positive effect on the IMF breeding value than do the Aa and aa genotypes for the MspI RFLP. In addition, the DD genotype has a significantly greater positive effect on IMF breeding value than the Dd and dd genotypes for the HaeIII RFLP. For the HinfI RFLP, the hh genotype has a significantly larger positive effect on IMF breeding value than the HH genotype. Multiple regression analysis was performed using the IMF breeding values as the dependent variable and the three H-FABP genotypes as independent variables. Results revealed that the contribution of the genotypes to variation in IMF breeding values was approximately 40%. These results demonstrated that H-FABP RFLPs affect IMF in this Duroc population.
 Keywords
Duroc Pigs;H-FABP;Intramuscular Fat;RFLP;
 Language
English
 Cited by
1.
Gene Expression of Heart and Adipocyte Fatty Acid-binding Protein in Chickens by FQ-RT-PCR,;;;;;;

Asian-Australasian Journal of Animal Sciences, 2010. vol.23. 8, pp.987-992 crossref(new window)
 References
1.
Gerbens, F., G. Rettenberger, J. A. Lenstra, J. H. Veerkamp and M. F. W. Te Pas. 1997. Characterization, chromosomal localization, and genetic variation of the porcine heart fatty acid-binding protein gene. Mammalian Genome. 8:328-332. crossref(new window)

2.
Gerbens, F., A. J. M. van Erp, F. L. Harders, F. J. Verburg, T .H. E. Meuwissen, J. H. Veerkamp and M. F. W. te Pas. 1999. Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs. J. Anim. Sci. 77:846-852.

3.
Gerbens, F., D. J. de Koning, F. L. Harders, T. H. E. Meuwissen, L. L. G. Janss, M. A. M. Groenen, J. H. Veerkamp, J. A. M. Van Arendonk and M. F. W. te Pas. 2000. The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content on Meishan crossbred pigs. J. Anim. Sci. 78:552-559.

4.
Gerbens, F., F. J. Verburg, H. T. B. Van Moerkerk, B. Engel, W. Buist, J. H. Veerkamp and M. F. W. te Pas. 2001. Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs. J. Anim. Sci. 79:347-354.

5.
Grindflek, E., J. Szyda, M. F. Rothschild and S. Lien. 2000. A QTL analysis and candidate gene study for meat quality on swine chromosome 6. International Plant and Animal Genome VIII Conference, San Diego, CA, January 9-12 (http://www. intl-pag.com/8/).

6.
Groeneveld, E. 1990. PEST User's Manual. Institute of Animal Husbandry and Animal Behaviour, Federal Agricultural Research Center, Braunsweig, Germany.

7.
Nechtelberger, D., V. Pires, J. Solkner, I. Stur, G. Brem, M. Mueller and S. Muelle. 2001. Intramuscular fat content and genetic variants at fatty acid-binding protein loci in Austrian pigs. J. Anim. Sci. 79:2798-2804.

8.
Neumaier, A. and E. Groeneveld. 1998. Restricted maximum likelihood estimation of covariances in sparse linear models. Genet. Sel. Evol. 30:3-26. crossref(new window)

9.
Oikawa, T., K. Suzuki, H. Kadowaki, T. Shibata and H. Uchida. 2002. Possibility of major genes and association of RYR1 gene to meat quality of Duroc selected for meat production and meat quality. 7th World Congress on Genetics Applied to Livestock Production, August 19-23, Montpelier, France. Communication No. 11-25.

10.
Ovilo, C., A. Oliver, J. L. Noguera, A. Clop, C. Barragan, L. Varona, C. Rodriguez, M. Toro, A. Sanchez, M. Perez-Enciso, and L. Silio. 2002. Test for positional candidate genes for body composition on pig chromosome 6. Genet. Sel. Evol. 34:465-479. crossref(new window)

11.
Suzuki, K., H. Kadowaki, T. Shibata, H. Uchida and A. Nishida. 2005. Selection for daily gain, loin-eye area, backfat thickness and intramuscular fat based on desired gains over seven generations of Duroc pigs. Livest. Prod. Sci. 97:193-202. crossref(new window)

12.
Yamada, Y., K. Yokouchi and A. Nishida. 1975. Selection index when genetic gains of individual traits are of primary concern. Japan. J. Genet. 50: 33-41. crossref(new window)

13.
Zeng, Y. Q., G. L. Wang, C. F. Wang, S. D. Wei, Y. Wu, L. Y. Wang, H. Wang and H. L. Yang. 2005. Genetic variation of H-FABP gene and association with intramuscular fat content in Laiwu Black and four western pig breeds. Asian-Aust. J. Anim. Sci. 18:13-16.