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The Porcine FoxO1, FoxO3a and FoxO4 Genes: Cloning, Mapping, Expression and Association Analysis with Meat Production Traits
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 Title & Authors
The Porcine FoxO1, FoxO3a and FoxO4 Genes: Cloning, Mapping, Expression and Association Analysis with Meat Production Traits
Yu, Jing; Zhou, Quan-Yong; Zhu, Meng-Jin; Li, Chang-Chun; Liu, Bang; Fan, Bin; Zhao, Shu-Hong;
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FoxO1, FoxO3a and FoxO4 belong to the FoxO gene family, which play important roles in the PI3K/PKB pathway. In this study, we cloned the porcine FoxO1, FoxO3a and FoxO4 sequences and assigned them to SSC11p11-15, SSC1p13 and SSC xq13 using somatic cell hybrid panel (SCHP) and radiation hybrid panel (IMpRH). RT-PCR results showed that these three genes are expressed in multiple tissues. Sequencing of PCR products from different breeds identified a synonymous T/C polymorphism in exon 2 of FoxO3a. This FoxO3a single nucleotide polymorphism (SNP) can be detected by AvaII restriction enzyme. The allele frequencies of this SNP were investigated in Dahuabai, Meishan, Tongcheng, Yushan, Large White, and Duroc pigs. Association of the genotypes with growth and carcass traits showed that different genotypes of FoxO3a were associated with carcass length and backfat thickness between 6th and 7th ribs (BTR) and drip loss (p<0.05).
Pig;FoxO1;FoxO3a;FoxO4;Mapping;Association Analysis;
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아세아태평양축산학회지, 2008. vol.21. 4, pp.499-509 crossref(new window)
Bodine, S. C., E. Latres, S. Baumhueter, V. K. M. Lai, L. Nunez, B. A. Clarke, W. T. Poueymirou, F. J. Panaro, E. Na, K. Dharmarajan, Z. Q. Pan, D. M. Valenzuela, T. M. DeChiara, T. N. Stitt, G. D. Yancopoulos and D. J. Glass. 2001. Identipcation of Ubiquitin Ligases Required for Skeletal Muscle Atrophy. Sci. 294:1704-1708. crossref(new window)

Eric, L. G. and B. Anne. 2005. FOXO transcription factors at the interface between longevity and tumor suppression. Oncogene. 24:7410-7425 crossref(new window)

Junger, M. A., F. Rintelen, H. Stocker, J. D. Wasserman, M. Vegh, T. Radimerski, M. E. Greenberg and E. Hafen. 2003. The Drosophila Forkhead transcription factor FOXO mediates the reduction in cell number associated with reduced insulin signaling. J. Biol. 2:20. crossref(new window)

Guan, H. P., B. Fan, K. Li, M. J. Zhu, M. Yerle and B. Liu. 2006. Sequence Characterization, Expression Profile, Chromosomal Localization and Polymorphism of the Porcine SMPX Gene. Asian-Aust. J. Anim. Sci. 19:937-941.

Gustavo, A. N. 2005. Molecular determinants of skeletal muscle mass: getting the "AKT" together. The Intern. J. Biochem. Cell Bio. 37:1985-1996. crossref(new window)

Jacobs, F. M., L. P. vander Heide, P. J. Wijchers, J. P. Burbach, M. F. Hoekman and M. P. Smidt. 2003. FoxO6, a novel member of the FoxO class of transcription factors with distinct shuttling dynamics. J. Biol. Chem. 278:35959-35967. crossref(new window)

Kaestner, K. H., W. Knochel and D. E. Martinez. 2000. Unified nomenclature for winged helix/forkhead transcription factors. Genes. Dev. 14:142-146.

Kamei, Y., S. Miura, M. Suzuki, Y. Kai, J. Mizukami, T. Taniguchi, K. Mochida, T. Hata, J. Matsuda, H. Aburatani, I. Nishino and O. Ezaki. 2004. Skeletal muscle FOXO1 (FKHR) transgenic mice have less skeletal muscle mass, down-regulated type I (Slow Twitch/Red Muscle) fiber genes, and impaired Glycemic Control. J. Biol. Chem. 279:41114-41123. crossref(new window)

Kramer, J. M., J. T. Davidge, J. M. Lockyer and B. E. Staveley. 2003. Expression of Drosophila FOXO regulates growth and can phenocopy starvation. BMC. Dev. Biol. 3:18

Masuko, K. and M. Katoh. 2004. Human FOX gene family. Int. J. Oncol. 25:1495-1500.

Milan, D., R. Hawken, C. Cabau, S. Leroux, C. Genet, Y. Lahbib, G. Tosser, A. Robic, F. Hatey, L. Alexander, C. Beattie, L. Schook, M. Yerle and J. Gellin. 2000. IMpRH server: an RH mapping server available on the Web. Bioinformatics. 16:558-559. crossref(new window)

Pan, P. W., S. H. Zhao, M. Yu, B. Liu, T. A. Xiong and K. Li. 2003. Identification of differentially expressed genes in the longissimus dorsi muscle tissue between duroc and Erhualian pigs by mRNA differential display. Asian-Aust. J. Anim. Sci. 16:1066-1070.

Paul, J. C. and M.T. Boudewijn Burgering. 2004. Forkhead-box transcription factors and their role in the immune system. Nature. 4:889-899.

Relling, D. P., L. B. Esberg, C. X. Fang, W. T. Johnson, E. J. Murphy, E. C. Carlson, J. T. Saari and J. Ren. 2006. High-fat diet-induced juvenile obesity leads to cardiomyocyte dysfunction and upregulation of Foxo3a transcription factor independent of lipotoxicity and apoptosis. J. Hypertens. 24:549-561. crossref(new window)

Sandri, M., C. Sandri, A. Gilbert, C. Skurk, E. Calabria, A. Picard, K. Walsh, S. Schiaffino, S. H. Lecker and A. L. Goldberg. 2004. Foxo Transcription Factors Induce the Atrophy-Related Ubiquitin Ligase Atrogin-1 and Cause Skeletal Muscle Atrophy. Cell. 117:399-412. crossref(new window)

Skurk, C., Y. Izumiya, H. Maatz, P. Rzaghi, I. Shiojima, M. Sandri, K. Sato, L. Zeng, S. Schiekofer, D. Pimentel, S. Lecker, H. Taegtmeyer, A. L. Goldberg and K. Walsh. 2005. The FOXO3a transcription factor regulates cardiac myocyte size downstream of AKT signaling. J. Biol. 280:20814-20823.

Stitt, T. N., D. Drujan, B. A. Clarke, F. Panaro, Y. Timofeyva, W. O. Kline, M. Gonzalez, G. D. Yancopoulos and D. J. Glass. 2004. The IGF-1/PI3K/AKT pathway prevents expression of muscle atrophy-induced ubiquitin ligases by inhibiting FOXO transcription factors. Mol. Cell. 14:359-403.

Tran, H., A. Brunet, E. C. Griffith and M. E. Greenberg. 2003. The many forks in FOXO's road. Science's STKE. 10:1126.

Trevor, N. S., D. Doreen, A. C Brian, P. Frank, T. Yekatarina, O. K. William, G. Michael, D. Y. George and J. G. David. 2004. The IGF-1/PI3K/Akt pathway prevents expression of muscle atrophy-Induced ubiquitin ligases by inhibiting FOXO transcription factors. Mol. Cell. 14:395-403. crossref(new window)

Wang, H. L., H. Wang, Z. M. Zhu, S. L. Yang, S. T. Feng and K. Li. 2006. Molecular Characterization and Expression Patterns of Porcine Eukaryotic Elongation Factor 1 A. Asian-Aust. J. Anim. Sci. 19:953-958.

Yang, J. E., M. Yu, B. Liu, B. Fan, M. Zhu, T. Xiong and K. Li. 2005. Cloning and initial analysis of porcine MPDU1 gene. Asian-Aust. J. Anim. Sci. 18:1237-1241.

Yerle, M., G. Echard, A. Robic, A. Mairal, C. Dubut-Fontana, J. Riquet, P. Pinton, D. Milan, Y. Lahbib-Mansais and J. Gellin. 1996. A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet. Cell. Genet. 73:194-202. crossref(new window)

Yerle, M., P. Pinton, A. Robic, A. Alfonso, Y. Palvadeau, C. Delcros, R. Hawken, L. Alexander, C. Beattie, L. Schook, D. Milan and J. Gellin. 1998. Construction of a whole-genome radiation hybrid panel for high resolution gene mapping in pigs. Cytogenet. Cell. Genet. 82:182-188. crossref(new window)