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Proteomic Comparison between Japanese Black and Holstein Cattle by Two-dimensional Gel Electrophoresis and Identification of Proteins
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 Title & Authors
Proteomic Comparison between Japanese Black and Holstein Cattle by Two-dimensional Gel Electrophoresis and Identification of Proteins
Ohsaki, H.; Okada, M.; Sasazaki, S.; Hinenoya, T.; Sawa, T.; Iwanaga, S.; Tsuruta, H.; Mukai, F.; Mannen, H.;
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Differences of meat qualities between Japanese Black and Holstein have been known in Japan, however, the causative proteins and/or the genetic background have been unclear. The aim of this study was to identify candidate proteins causing differences of the meat qualities between the two breeds. Using technique of two-dimensional gel electrophoresis, protein profiling was compared from samples of the longissimus dorsi muscle and subcutaneous adipose tissue. Five protein spots were observed with different expression levels between breeds. By using LC-MS/MS analysis and Mascot program, three of them were identified as ankyrin repeat protein 2, phosphoylated myosin light chain 2 and mimecan protein. Subsequently, we compared the DNA coding sequences of three proteins between breeds to find any nucleotide substitution. However, there was no notable mutation which could affect pI or molecular mass of the proteins. The identified proteins may be responsible for different characteristics of the meat qualities between Japanese Black and Holstein cattle.
Cattle;Meat Quality;Two-dimensional Gel Electrophoresis;LC-MS/MS;Proteome;
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Bouley, J., B. Meunier, C. Chambon, S. De Smet, J. F. Hocquette and B. Picard. 2005. Proteomic analysis of bovine skeletal muscle hypertrophy. Proteomics 5:490-500. crossref(new window)

Bozzo, C., L. Stevens, L. Toniolo, Y. Mounier and C. Reggiani. 2003. Increased phosphorylation of myosin light chain associated with slow-to-fast transition in rat soleus. Am. J. Physiol. Cell Physiol. 285:575-583. crossref(new window)

Edgar, P. F., J. E. Douglas, C. Knight, G. J. Cooper, R. L. Faull and R. Kydd. 1999. Proteome map of the human hippocampus. Hippocampus 9:644-650. crossref(new window)

Funderburgh, J. L., L. M. Corpuz, M. R. Roth, M. L. Funderburgh, E. S. Tasheva and G. W. Conrad. 1997. Mimecan, the 25-kDa corneal keratan sulfate proteoglycan, is a product of the gene producing osteoglycin. J. Biol. Chem. 272:28089-28095. crossref(new window)

Gygi, S. P., G. L. Corthals, Y. Zhang, Y. Rochon and R. Aebersold. 2000. Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology. Proc. Natl. Acad. Sci. USA 97:9390-9395. crossref(new window)

Hu, S. M., F. Li, H. M. Yu, R. Y. Li, Q. Y. Ma, T. J. Ye, Z. Y. Lu, J. L. Chen and H. D. Song. 2005. The mimecan gene expressed in human pituitary and regulated by pituitary transcription factor-1 as a marker for diagnosing pituitary tumors. J. Clin. Endocrinol. Metab. 90:6657-6664. crossref(new window)

Iwamoto, H., Y. Ono, T. Goto, S. Nishimura, Y. Nakanishi, R. Umezu and H. Takahara. 1991. Comparative studies on the composition of muscle fiber types in Japanese Black, Japanese Brown and Holstein steers. Anim. Sci. Technol. 62:674-682.

Jung, K. C., S. L. Yu, Y. J. Lee, K. D. Choi, J. S. Choi, Y. H. Kim, B. G. Jang, S. H. Kim, D. H. Hahm and J. H. Lee. 2005. Muscle Proteome Analysis for the Effect of Panax Ginseng Extracts in Chicken: Identification of Proteins Using Peptide Mass Fingerprinting. Asian-Aust. J. Anim. Sci. 18:922-926. crossref(new window)

Kemp, T. J., T. J. Sadusky, F. Saltisi, N. Carey, J. Moss, S. Y. Yang, D. A. Sassoon, G. Goldspink and G. R. Coulton. 2000. Identification of Ankrd2, a Novel Skeletal Muscle Gene Coding for a Stretch-Responsive Ankyrin-Repeat Protein. Genomics 66:229-241. crossref(new window)

Mckoy, G., Y. Hou, S. Y. Yang, D. Vega Avelaira, H. Degens, G. Goldspink and G. R. Coulton. 2005. Expression of Ankrd2 in fast and slow muscles and its response to stretch are consistent with a role in slow muscle function. J. Appl. Physiol. 98:2337-2343. crossref(new window)

Odahara, S., H. J. Chung, S. H. Choi, S. L. Yu, S. Sasazaki, H. Mannen, C. S. Park and J. H. Lee. 2006. Mitochondrial DNA Diversity of Korean Native Goats. Asian-Aust. J. Anim. Sci. 19:482-485. crossref(new window)

Pappin, D. J., P. Hojrup and A. J. Bleasby. 1993. Rapid identification of proteins by peptide-mass fingerprinting. Curr. Biol. 3:327-332. crossref(new window)

Pyo, J., S. I. Hwang, J. Oh, S. Lee, S. Kang, J. Kim and J. Lim. 2003. Characterization of a bovine pregnancy-associated protein using two-dimensional gel electrophoresis, N-terminal sequencing and mass spectrometry. Proteomics 3:2420-2427. crossref(new window)

Sasazaki, S., S. Odahara, C. Hiura, F. Mukai and H. Mannen. 2006. Mitochondrial DNA Variation and Genetic Relationships in Japanese and Korean Cattle. Asian-Aust. J. Anim. Sci. 19:1394-1398. crossref(new window)

Taniguchi, M., H. Mannen, K. Oyama, Y. Shimakura, A. Oka, H. Watanabe, T. Kojima, M. Komatsu, G. S. Harper and S. Tsuji. 2004. Differences in stearoyl-CoA desaturase mRNA levels between Japanese Black and Holstein cattle. Livest. Prod. Sci. 87:215-220. crossref(new window)

Tasheva, E. S., A. Koester, A. Q. Paulsen, A. S. Garrett, D. L. Boyle, H. J. Davidson, M. Song, N. Fox and G. W. Conrad. 2002. Mimecan/osteoglycin-deficient mice have collagen fibril abnormalities. Mol. Vis. 8:407-415.

Yoon, S. W., T. Y. Kim, M. H. Sung, C. J. Kim and H. Poo. 2005. Comparative proteomic analysis of peripheral blood eosinophils from healthy donors and atopic dermatitis patients with eosinophilia. Proteomics 5:1987-1995. crossref(new window)