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Effect of Essential and Nonessential Amino Acids in North Carolina State University (NCSU)-23 Medium on Development of Porcine In vitro Fertilized Embryos
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 Title & Authors
Effect of Essential and Nonessential Amino Acids in North Carolina State University (NCSU)-23 Medium on Development of Porcine In vitro Fertilized Embryos
Hashem, Md. Abul; Bhandari, Dilip P.; Hossein, Mohammad Shamim; Jeong, Yeon Woo; Kim, Sue; Kim, Ji-Hye; Koo, Ok-Jae; Park, Seon Mi; Lee, Eu Gine; Park, Sun Woo; Kang, Sung Keun; Lee, Byeong Chun;
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The present study was conducted to examine the effect of different levels of essential and nonessential amino acid in NCSU-23 medium on the in vitro-produced porcine embryo as it develops from the zygote to the blastocyst stage. Four experiments were performed, each with a completely randomized design involving 5 to 8 replications of treatments. In order to know the effect of nonessential amino acids in NCSU-23 medium, 0, 5, 10 and MEM were supplemented there to, (Exp. 1) and the medium was supplemented with same level of essential amino acids (Exp. 2). The combined effect of nonessential (0, 5, 10 and MEM) and essential amino acids (0, 5, 10 and MEM) in NCSU-23 medium (Exp. 3), first 72 h with non-essential amino acids (at 0, 5, 10 and MEM), and last 4 d with essential amino acids with the same level as NEAA (Exp. 4) were examined. The embryo development was monitored and the quality of blastocysts was evaluated by counting the number of total cells and determining the ratio of inner cell mass (ICM) to trophoectoderm (TE) cells. When Eagle's nonessential amino acids (MEM) added to NCSU-23 medium, it significantly increased the likelihood of development to the 2- to 4-cell stage and subsequent blastocyst development. Supplementation of different levels of essential amino acids in the NCSU-23 medium decreased cleavage rate, rate of morula and blastocyst development and the number of ICMs. In the case of the combined effect of essential and nonessential amino acids, better and significant results were found for blastocysts, hatching blastocysts and for ICM numbers which were also dose dependent. With respect to the biphasic effect of nonessential and essential amino acids, nonessential amino acids increased cleavage whereas essential amino acids increased the total cell number. Neither the nonessential nor the essential group of amino acids, on their own, affected blastocyst cell number or the differentiation of cells in the blastocyst. In conclusion, this study determined the role of nonessential and essential amino acids in the culture of the porcine embryo and showed that the embryo requires different levels of amino acids as it develops from the zygote to the blastocyst stage.
NCSU-23;Essential;Nonessential;Amino Acids;Porcine;IVF;
 Cited by
Alexiou, M. and H. J. Leese. 1992. Purine utilisation, de novo synthesis and degradation in mouse preimplantation embryos. Development. 114:185-192.

Bavister, B. D. and T. Arlotto. 1990. Influence of single amino acids on the development of hamster 1-cell embryos in vitro. Mol. Reprod. Dev. 25:45-51. crossref(new window)

Bavister, B. D. and S. H. McKiernan. 1993. Regulation of hamster embryo development in vitro by amino acids. In Preimplantation Embryo Development (Ed. B. D Bavister). New York: Springer-Verlag. pp. 57-72.

Biggers, J. D., L. McGinnis and M. Raffin. 2000. Amino acids and preimplantation development of the mouse in protein-free potassium simplex optimised medium. Biol. Reprod. 63:281-293. crossref(new window)

Carney, E. W. and B. D. Bavister. 1987. Stimulatory and inhibitory effects of amino acids on the development of hamster eightcell embryos in vitro. J. In vitro. Fert. Embryo. Transfer. 4:162-167. crossref(new window)

Dawson, K. M., J. L. Collins and J. M. Baltz. 1998. Osmolaritydependent glycine accumulation indicates a role for glycine as an organic osmolyte in early preimplantation mouse embryos. Biol. Reprod. 59:225-232. crossref(new window)

Dumoulin, J. C., L. C. Van Wissen, P. P. Menheere, A. H. Michiels, J. P. Geraedts and J. L. Evers. 1997. Taurine acts as an osmolyte in human and mouse oocytes and embryos. Biol. Reprod. 56:739-744. crossref(new window)

Edwards, L. J., D. A. Williams and D. K. Gardner. 1998. Intracellular pH of the mouse preimplantation embryo: amino acids act as buffers of intracellular pH. Hum. Reprod. 13:3441-3448. crossref(new window)

Epstein, C. J. and S. A. Smith. 1973. Amino acid uptake and protein synthesis in preimplantation mouse embryos. Develop. Biol. 33:171-184. crossref(new window)

Gardner, D. K. 1994. Mammalian embryo culture in the absence of serum or somatic cell support. Cell Biol. Int. 18:1163-1179. crossref(new window)

Gardner, D. K. 1998. Changes in requirements and utilisation of nutrients during mammalian preimplantation embryo development and their significance in embryo culture. Theriogenol. 49:83-102. crossref(new window)

Gardner, D. K. and M. Lane. 1993. Amino acids and ammonium regulate the development of preimplantation mouse embryos in culture. Biol. Reprod. 48:377-385. crossref(new window)

Gardner, D. K. and M. Lane. 1996. Alleviation of the '2-cell block' and development to the blastocyst of CF1 mouse embryos: role of amino acids, EDTA and physical parameters. Hum. Reprod. 11:2703-2712.

Houghton, F. D., J. A. Hawkhead, P. G. Humpherson, J. E. Hogg, A. H. Balen, A. J. Rutherford and H. J. Leese. 2002. Noninvasive amino acid turnover predicts human embryo developmental capacity. Hum. Reprod. 17:99-1005. crossref(new window)

Jang, H. Y., H. S. Kong, K. D. Choi, G. J. Jeon, B. K. Yang, C. K. Lee and H. K. Lee. 2005. Effects of melatonin on gene expression of IVM/IVF porcine embryos. Asian-Aust. J. Anim. Sci. 18:17-21.

Ka, H. and F. W. Bazer. 2005. Effects of keratinocyte growth factor on the uterine endometrial epithelial cells in pigs. Asian-Aust. J. Anim. Sci. 18:1708-1714.

Katchadourian, C., C. Joly and Y. Menezo. 1994. Interactions in glycine and methionine uptake, conversion and incorporation into proteins in the preimplantation mouse embryo. Zygote. 2:301-306.

Kim, J. H., K. Niwa, J. M. Lim and K. Okuda. 1993. Effects of phosphate, energy substrates, and amino acids on development of in vitro-matured, in vitro-fertilized bovine oocytes in a chemically defined, protein-free culture medium. Biol. Reprod. 48:1320-1325. crossref(new window)

Lamb, V. K. and H. J. Leese. 1994. Uptake of a mixture of amino acids by mouse blastocysts. J. Reprod. Fertil. 120:169-175. crossref(new window)

Lane, M. and D. K. Gardner. 1994. Increase in postimplantation development of cultured mouse embryos by amino acids and induction of fetal retardation and exencephaly by ammonium ions. J. Reprod. Fertil. 102:305-312. crossref(new window)

Lane, M. and D. K. Gardner. 1997. EDTA stimulates the development of cleavage stage mouse embryos by inhibiting the glycolytic enzyme phosphoglycerate kinase. Biol. Reprod. 57:193.

Lane, M. and D. K. Gardner. 1997. Non essential amino acids and glutamine decrease the time of the first three cleavage divisions and increase compaction of mouse zygotes in vitro. J. Assist. Reprod. Genet. 14:398-403. crossref(new window)

Lane, M., and D. K. Gardner. 1997a. Differential regulation of mouse embryo development and viability by amino acids. J. Reprod. Fertil. 109:153-164. crossref(new window)

Lane, M. and Gardner. 1998. Amino acids and vitamins prevent culture-induced metabolic perturbations and associated loss of viability of mouse blastocysts. Hum. Reprod. 13:991-997. crossref(new window)

Lane, M., K. Hooper and D. K. Gardner. 2001. Effect of essential amino acids on mouse embryo viability and ammonium production. J. Assist. Reprod. Genet. 18:519-525. crossref(new window)

Lindenbaum, A. 1973. A survey of naturally occurring chelating ligands. Advan. Experim. Med. Biol. 40:67-77.

Martin, P. M. and A. E. Sutherland. 2001. Exogenous amino acids regulate trophectoderm differentiation in the mouse blastocyst through an mTOR-dependent pathway. Develop. Biol. 240:182-193. crossref(new window)

McKiernan, S. H., K. Murray and B. D. Bavister. 1995. Analysis of stimulatory and inhibitory amino acids for development of hamster one-cell embryos in vitro. Mol. Reprod. Dev. 42:188-199. crossref(new window)

Mehta, T. S. and A. A. Kiessling. 1990. Development potential of mouse embryos conceived in vitro and cultured in ethylenediaminetetraacetic acid with or without amino acids or serum. Biol. Reprod. 43:600-606. crossref(new window)

Nasr-Esfahani, M. H., N. J. Winston and M. H. Johnson. 1992. Effects of glucose, glutamine, ethylenediaminetetraacetic acid and oxygen tension on the concentration of reactive oxygen species and on the development of the mouse preimplantation in vitro. J. Reprod. Fertil. 96:219-231. crossref(new window)

Niwa, K. and H. Funahashi. 1999. Recent Development in Embryo Technology in Pigs.-A review. Asian-Aust. J. Anim. Sci. 12:966.

Papaioannou, V. E. and K. M. Ebert. 1986. Development of fertilized embryos transferred to oviducts of immature mice. J. Repod. Fertil. 76:603-608. crossref(new window)

Rosenkrans, C. F. J. and N. L. First. 1994. Effect of free amino acids and vitamins on cleavage and developmental rate of bovine zygotes in vitro. J. Anim. Sci. 72:434-437.

Schini, S. A. and B. D. Bavister. 1990. Development of golden hamster embryos through the subsequent implantation in vivo. Biol. Reprod. 42:662-668.

Steeves, T. E. and D. K. Gardner. 1999. Temporal and differential effects of amino acids on bovine embryo development in culture. Biol. Reprod. 61:731-740. crossref(new window)

Takahashi, Y. and N. L. First. 1992. In vitro development of bovine one-cell embryos: influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenol. 37:963-978. crossref(new window)

Van Winkle, L. J. and A. L. Campione. 1996. Amino acid transport regulation in preimplantation mouse embryos: effect of amino acid content and pre- and peri-implantation development. Theriogenol. 45:69-80. crossref(new window)

Watson, A. J., P. De Sousa, A. Caveney, L. C. Barcroft, D. Natale, J. Urquhart and M. E. Westhusin. 2000. Impact of bovine oocyte maturation media on oocyte transcript levels, blastocyst development, cell number, and apoptosis. Biol. Reprod. 62:355-364. crossref(new window)

Wu, G. and S. M. Morris. 1998. Arginine metabolism: nitric oxide and beyond. Biochem. J. 336:1-17.

Zhang, X. and D. T. Armstrong. 1990. Presence of amino acids and insulin in a chemically defined medium improves development of 8-cell rat embryos in vitro and subsequent implantation in vivo. Biol. Reprod. 42:662-668. crossref(new window)