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Effect of rc Mutation on Semen Characteristics, Spermatogenic Tissues and Testosterone Profile in Blind Rhode Island Red Cockerels
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Effect of rc Mutation on Semen Characteristics, Spermatogenic Tissues and Testosterone Profile in Blind Rhode Island Red Cockerels
Arshami, J.; Cheng, K.;
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Seven rc mutant and seven normal male birds (Rhode Island Red suie, RIR) were used in this study to determine the effects of rc mutation on semen characteristics, testosterone profile and spermatogenic tissues. All birds were randomly selected at week 12 of age and housed in individual cages and were fed and watered ad libitum. The birds were exposed to a 14L:10D light cycle during experiment. Semen were collected at weeks 22 to 23 from each bird twice a week and evaluated for semen volume (SV), sperm concentration (SC), total sperm count (TSC), percent of sperm motility (%SM), dead sperm (%DS), and sperm metabolic activity (SMA). To determine the testosterone concentration (TC) in plasma, blood was collected at weeks 12, 16 and 18. Testicular tissue were collected, processed and evaluated for semineferous tubule diameter (STD), round spermatid number (RSN), percent elongated sperm (%ES) and semineferous tubules length (STL). Body weight (BW), comb weight (CW) and testes weight (TW) were weighted at the end of experiment (week 23). The SV, TSC and %SM were significantly higher in normal birds but the %DS was higher in blind birds (p<0.05). The SC did not differ significantly between the two groups but its value was higher in normal birds. The sperm metabolic activity in the first h of collection did not differ significantly between the two groups but after 24 h, the level of SMA in normal group was significantly higher (p<0.05). The level of TC did not differ significantly between the two genotype groups but normal birds had higher TC in all collections except the last one. The STD, RSN, %ES and STL in normal birds were higher when compared to blind birds but the differences were insignificant except for ES percent. The BW, CW and TW between the two groups did not differ significantly but the weights were higher in normal group compared to blind birds. Statistical analysis of semen characteristics, testosterone profile and histological factors were indicated detrimental effects of rc mutation in prepubertal RIR blind male birds due to lack of light.
rc Mutation;Semen Characteristics;Spermatogenic Tissues;Testosterone Hormone;Blind Cockerel;
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아세아태평양축산학회지, 2009. vol.22. 5, pp.618-625 crossref(new window)
Arshami, J. and J. L. Ruttle. 1998. Effects of diets containing gossypol on spermatogenic tissues of young bulls. Theriogenol. 30:507-516.

Ashizawa, K. and G. H. Wishart. 1987. Resolution of the sperm motility stimulating principle of fowl seminal plasma into $Ca^{+}^{+}$ and an unidentified low molecular weight factor. J. Reprod. Fertil. 81:495-499 crossref(new window)

Becker, W. M. and D. W. Deamer. 1991. The world of the cell (2nd. Ed) in: The Benjamin/Cummings Publishing Co., Inc. California, USA.

Blazak, W. F. and N. S. Fechheimer. 1981. Testicular sperm reserves in cockerels bearing Z-autosome translocations. J. Poult. Sci. 60:2001-2005. crossref(new window)

Brillard, J. P. and G. R. McDaniels. 1986. Influence of spermatozoa numbers and insemination frequency on fertility in dwarf broiler breeder hens. J. Poult. Sci. 65:2330-2334. crossref(new window)

Burrows, W. H. and J. P. Quinn. 1937. The collection of spermatozoa from the domestic fowl and turkey. J. Poult. Sci. 16:19-24. crossref(new window)

Cerruti-Sola, S., M. Castagnaro and K. M. Cheng. 1997. Histological changes caused by the rc mutation in chickens. J. Comp. Path. 116:329-338. crossref(new window)

Chaudhuri, D. and G. J. Wishart. 1988. Predicting the fertilizing ability of avian semen: Development of an objective colorimetric method for assessing the metabolic activity of fowl spermatozoa. J. Br. Poult. Sci. 29:837-845. crossref(new window)

Cheng, K. M. and S. F. Pang. 1989. The rc gene, photoreceptors, and melatonin in the chicken. Advanced in pineal research: R. J. Reiter and S.F. Pang Eds., John Libbey and Co Ltd. pp. 83-86.

Cheng, K. M., R. N. Shoffner, K. N. Gelatt and G. G. Gum. 1978. An induced retinal mutation (rc) in the chicken. J. Poult. Sci. 57:1127-1132.

Cheng, K. M., R. N. Shoffner, K. N. Gelatt, G. G. Gum, J. S. Otis and J. J. Bitgood. 1980. An autosomal recessive blind mutant in the chicken. J. Poult. Sci. 9:2179-2128.

Froman, D. P. and D. J. McLean. 1996. Objective measurement of sperm motility based upon penetration of Accudenz 1. J. Poult. Sci. 75:776-784. crossref(new window)

Garier, D. H. and J. Attal. 1970. Variations de la testosterone du plasma testicular et des cellules interstitiels chez le canard pekin au cours du cycle annuel. Compt. Rend. Acad. Sci. (Paris). 270:2472-2486.

Kerlan, J. T. and R. B. Jaffe. 1974. Plasma testosterone levels during the testiculer cycle of the red-winged blackird (Agelaius phoeniceus). Gen. Comp. Endocrinol. 22:428-432. crossref(new window)

Kumoran, J. D. S. and C. W. Turner. 1949. The normal development of the testes in the White Plymouth Rock. J. Poult. Sci. 28:511-520. crossref(new window)

Lake, P. E. and I. A. Furr. 1971. The endocrine testis in reproduction. In: Physiology and Biochemistry of the Domestic Fowl. Vol. 3. (Ed. D. J. Bell and B. M. Freeman) Academic Press, New York Chapter 62

Lehniger, A. L., D. L. Nelson and M. M. Cox. 1993. Principles of Biochemistry (2nd ed). Worth Publishers, New York.

Marini, P. J. and B. L. Goodman. 1969. Semen characteristics as influenced by selection of divergent growth rate in chickens. J. Poult. Sci. 48:85-89.

Mather, F. B. and W. O. Wilson. 1964. Post-natal testicular development in Japanese Quail (Coturnix japonica). J. Poult. Sci. 43:860-864. crossref(new window)

Mortimer, D. 1994. Practical Laboratory Andrology. Oxford University press. New York.

Pelech, S. L., E. Power and D. E. Vance. 1983. Activities of the phosphatidylcholine biosynthetic enzymes in rat liver during development. Can. J. Biotch. Cell Biol. 61:1147-1152. crossref(new window)

Poccia, D. 1994. Molecular Biology Intelligence Unit: Molecular Aspects of Spermatogenesis. R. G. Landes Co. Austin pp. 8-15.

Rabkin, S. W. and K. M. Cheng. 1992. A genetic abnormality of cardiac myocytes from the blind mutant (rc) chick heart: abnormalities of cardiac structure and choline transport. Basic Res. Cardiol. 87:610-617. crossref(new window)

Romanov, M. N. 2001. Genetics of broodiness in poultry - a review. Asian-Aust. J. Anim. Sci. 14:1647-1654. crossref(new window)

Ruckebusch, Y., L. Phaneuf and R. Dunlop. 1991. Physiology of small and large animals. B. C. Decker Inc. Philadelphia pp. 254-255.

SAS. 1988. SAS Procedure Guid, Release 6.03 ed. SAS Institute Inc., Cary, NC, USA.

Schanbacher, B. D., W. R. Gomes and N. L. Van Demark. 1974. Diurnal rhythm in serum testosterone levels and thymidine uptake by testes in the domestic fowl. J. Anim. Sci. 38:1245-1258. crossref(new window)

Semple-Rowland, S. L., W. A. Gorczyca, J. Buczylko, B. S. Helekar, C. C. Ruiz, I. Subbaraya, K. Palczewski and W. Baehr. 1996. Expression of CGAP1 and CGAP2 in the retinal degeneration (rc) mutant chicken retina. FEBS Letters. 385:47-52. crossref(new window)

Sexton, T. J. 1977. A new Poultry Extender: 1. Effect of extension on the fertility of chicken semen. J. Poult. Sci. 56:1443-1446. crossref(new window)

Steel, R. G. D. and J. H. Torrie. 1986. Principles and Procedures of Statistics. International Student Ed., McGraw Hill, Tokyo.

Temple, S. A. 1974. Plasma testosterone titers during the annual reproductive cycle of starlings (Sturnus vulgaris). Gen. Comp. Endocrinol. 22:470-282. crossref(new window)

Wilcox, F. H. 1959. Effect of the addition of carbohydrates after storage on the motility and fertilizing ability of chicken sperm. J. Poult. Sci. 38:1162-1168. crossref(new window)