Advanced SearchSearch Tips
Effect of Oocyte Activation Regimens on Ploidy of Nuclear Transfer Embryos Reconstructed with Fetal Fibroblasts in Rabbit
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Oocyte Activation Regimens on Ploidy of Nuclear Transfer Embryos Reconstructed with Fetal Fibroblasts in Rabbit
Yoo, Jae-Gyu; Rho, Gyu-Jin;
  PDF(new window)
Considerable attention has been focused on the cloning of mammalian embryos, as a consequence of poor development, in order to enhance the application of genetic engineering. Experiments were conducted to compare the developmental competence of parthenotes and reconstructed (NT) rabbit eggs with fetal fibroblasts (FFs) following various activation regimens. Oocytes and NT eggs were exposed to: electric stimulation (EST, Group 1) and EST followed by 6-dimethylaminopurine (DMAP, Group 2), cycloheximide (CHX, Group 3) or DMAP/CHX (Group 4). Pronuclear (PN) status, cleavage, blastocyst development and the ploidy were assessed. In parthenote groups 1, 2, 3 and 4, the PN formation differed significantly. And, the cleavage and blastocyst rates were 41.7 and 5%, 75.6 and 53.7%, 68 and 36%, 82.1 and 52.6%, respectively, among treatments. Polyploidy was observed in 17.2% of EST plus DMAP and 44.9% of EST plus DMAP/CHX groups. In SCNT groups (Group 1, 2, 3 and 4), the cleavage and blastocyst rates were 28.6 and 7.1%, 58.3 and 29.2%, 56.8 and 24.1%, 64.5 and 27.8%, respectively. The chromosomal composition differed significantly (p<0.05) among treatments. In Group 2 and 3, 53.8% and 81.8% of embryos revealed diploid chromosomal sets, respectively. However, in Group 4, 53.3% of embryos showed abnormal ploidy (mixoploid). Although DMAP or combination with DMAP/CHX resulted in higher in vitro development of rabbit SCNT embryos, higher incidence of chromosomal abnormality may induce problems related to fetal loss of at late stage of development.
Activation;Nuclear Transfer;Development;Ploidy;Rabbit;
 Cited by
Effects of Donor Cell Passage, Size and Type on Development of Porcine Embryos Derived from Somatic Cell Nuclear Transfer,;;;;;;;;

아세아태평양축산학회지, 2009. vol.22. 2, pp.194-200 crossref(new window)
Bhak, J. S., S. L. Lee, S. A. Ock, B. Mohana Kumar, S. Y. Choe and G. J. Rho. 2006. Developmental rate and ploidy of embryos produced by nuclear transfer with different activation treatments in cattle. Anim. Reprod. Sci. 92:37-49. crossref(new window)

Bos-Mikich, A., K. Swann and D. G. Whittingham. 1995. Calcium oscillations and protein synthesis inhibition synergistically activate mouse oocytes. Mol. Reprod. Dev. 41:84-90. crossref(new window)

Bureau, W. S., V. Bordignon, C. Leveillee, L. C. Smith and W.A. King. 2003. Assessment of chromosomal abnormalities in bovine nuclear transfer embryos and in their donor cells. Cloning Stem Cells 5:123-132. crossref(new window)

Cervera, R. P. and F. Garcia-Ximenez. 2004. Effects of the time interval between fusion and activation on in vitro rabbit nuclear transfer efficiency when nuclear donor cells are derived from older adults. Zygote 12:133-141. crossref(new window)

Chesne, P., P. G. Adenot, C. Viglietta, M. Baratte, L. Boulanger and J. P. Renard. 2002. Cloned rabbits produced by nuclear transfer from adult somatic cells. Nat. Biotechnol. 20:366-369. crossref(new window)

Collas, P., T. Chang, C. Long and J. M. Robl. 1995. Inactivation of histone H1 kinase by Ca2+ in rabbit oocytes. Mol. Reprod. Dev. 40:253-258. crossref(new window)

Deng, M. Q. and S. S. Shen. 2000. A specific inhibitor of p34(cdc2)/cyclin B suppresses fertilization-induced calcium oscillations in mouse eggs. Biol. Reprod. 62:873-878. crossref(new window)

Dinnyes, A., Y. Dai, M. Barber, L. Liu, J. Xu, P. Zhou and X. Yang. 2001. Development of cloned embryos from adult rabbit fibroblasts: effect of activation treatment and donor cell preparation. Biol. Reprod. 64:257-263. crossref(new window)

Kanayama, K., T. Sankai, K. Nariai, T. Endo and Y. Sakuma. 1994. Simplification of superovulation induction by using polyvinylpyrrolidone as a solvent for FSH in rabbits. J. Vet. Med. Sci. 56:599-600. crossref(new window)

Keefer, C. L. 2004. Production of bioproducts through the use of transgenic animal models. Anim. Reprod. Sci. 82-83:5-12. crossref(new window)

Kim, Y. S., S. L. Lee, S. A. Ock, S. Balasubramanian, S. Y. Choe and G. J. Rho. 2005. Development of cloned pig embryos by nuclear transfer following different activation treatments. Mol. Reprod. Dev. 70:308-313. crossref(new window)

Li, S., X. Chen, Z. Fang, J. Shi and H. Z. Sheng. 2006. Rabbits generated from fibroblasts through nuclear transfer. Reprod. 131:1085-1090. crossref(new window)

Liu, J. L., L. Y. Sung, F. Du, M. Julian, S. Jiang, M. Barber, J. Xu, X. C. Tian and X. Yang. 2004. Differential development of rabbit embryos derived from parthenogenesis and nuclear transfer. Mol. Reprod. Dev. 68:58-64. crossref(new window)

Liu, L., J. C. Ju and X. Yang. 1998. Differential inactivation of maturation-promoting factor and mitogen-activated protein kinase following parthenogenetic activation of bovine oocytes. Biol. Reprod. 59:537-545. crossref(new window)

Liu, S. Z., M. X. Jiang, L. Y. Yan, Y. Jiang, Y. C. Ouyang, Q. Y. Sun and D. Y. Chen. 2005. Parthenogenetic and nuclear transfer rabbit embryo development and apoptosis after activation treatments. Mol. Reprod. Dev. 72:48-53. crossref(new window)

McCreath, K. J., J. Howcroft, K. H. Campbell, A. Colman, A. E. Schnieke and A. J. Kind. 2000. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature 405:1066-1069. crossref(new window)

Moos, J., P. E. Visconti, G. D. Moore, R. M. Schultz and G. S. Kopf. 1995. Potential role of mitogen-activated protein kinase in pronuclear envelope assembly and disassembly following fertilization of mouse eggs. Biol. Reprod. 53:692-699. crossref(new window)

Neant, I. and P. Guerrier. 1988. 6-Dimethylaminopurine blocks starfish oocyte maturation by inhibiting a relevant protein kinase activity. Exp. Cell. Res. 176:68-79. crossref(new window)

Onodera, M. and Y. Tsunoda. 1989. Parthenogenetic activation of mouse and rabbit eggs by electric stimulation in vitro. Gamete. Res. 22:277-283. crossref(new window)

Ozil, J. P. 1990. The parthenogenetic development of rabbit oocytes after repetitive pulsatile electrical stimulation. Development 109:117-127.

Rho, G. J., B. Wu, S. Kawarsky, S. P. Leibo and K. J. Betteridge. 1998. Activation regimens to prepare bovine oocytes for intracytoplasmic sperm injection. Mol. Reprod. Dev. 50:485-492. crossref(new window)

Roh, S., N. Malakooti, J. R. Morrison, A. O. Trounson and Z. T. Du. 2003. Parthenogenetic activation of rat oocytes and their development (in vitro). Reprod. Fertil. Dev. 15:135-140. crossref(new window)

Slimane-Bureau, W. C. and W. A. King. 2002. Chromosomal abnormalities: a potential quality issue for cloned cattle embryos. Cloning Stem Cells 4:319-329. crossref(new window)

Soloy, E., J. Kanka, D. Viuff, S. D. Smith, H. Callesen and T. Greve. 1997. Time course of pronuclear deoxyribonucleic acid synthesis in parthenogenetically activated bovine oocytes. Biol. Reprod. 57:27-35. crossref(new window)

Stice, S. L. and J. M. Robl. 1988. Nuclear reprogramming in nuclear transplant rabbit embryos. Biol. Reprod. 39:657-64. crossref(new window)

Susko-Parrish, J. L., M. L. Leibfried-Rutledge, D. L. Northey, V. Schutzkus and N. L. First. 1994. Inhibition of protein kinases after an induced calcium transient causes transition of bovine oocytes to embryonic cycles without meiotic completion. Dev. Biol. 166:729-39. crossref(new window)

Wakayama, T., A. C. Perry, M. Zuccotti, K. R. Johnson and R. Yanagimachi. 1998. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374. crossref(new window)

Wells, D. N., P. M. Misica and H. R. Tervit. 1999. Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol. Reprod. 60:996-1005. crossref(new window)

Willadsen, S. M. 1986. Nuclear transplantation in sheep embryos. Nature 320:63-65. crossref(new window)

Wilmut, I., A. E. Schnieke, J. McWhir, A. J. Kind and K. H. Campbell. 1997. Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813. crossref(new window)

Yang, X., G. A. Presicce, L. Moraghan, S. E. Jiang and R. H. Foote. 1994. Synergistic effect of ethanol and cycloheximide on activation of freshly matured bovine oocytes. Theriogenol. 41:395-403. crossref(new window)

Yin, X. J., T. Tani, Y. Kato and Y. Tsunoda. 2000. Development of rabbit parthenogenetic oocytes and nuclear-transferred oocytes receiving cultured cumulus cells. Theriogenol. 54:1469-1476. crossref(new window)

Yoo, J. G., S. Y. Choe and G. J. Rho. 2003. Efficient production of cloned bovine embryos using cdc2 kinase inhibitor. Reprod. Domest. Anim. 38:444-450. crossref(new window)

Zhou, Q., J. P. Renard, G. Le Friec, V. Brochard, N. Beaujean, Y. Cherifi, A. Fraichard and J. Cozzi. 2003. Generation of fertile cloned rats by regulating oocyte activation. Sci. 302:1179. crossref(new window)