JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Intramuscular Administration of Zinc Metallothionein to Preslaughter Stressed Pigs Improves Anti-oxidative Status and Pork Quality
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Intramuscular Administration of Zinc Metallothionein to Preslaughter Stressed Pigs Improves Anti-oxidative Status and Pork Quality
Li, L.L.; Hou, Z.P.; Yin, Y.L.; Liu, Y.H.; Hou, D.X.; Zhang, B.; Wu, G.Y.; Kim, S.W.; Fan, M.Z.; Yang, C.B.; Kong, X.F.; Tang, Z.R.; Peng, H.Z.; Deng, D.; Deng, Z.Y.; Xie, M.Y.; Xiong, H.; Kang, P.; Wang, S.X.;
  PDF(new window)
 Abstract
This study was conducted to determine the effects of exogenous zinc-metallothionein (Zn-MT) on anti-oxidative function and pork quality. After feeding a corn-soybean meal-based diet for two weeks, 48 pigs () were assigned randomly to four groups. Pigs in Group 1 were maintained under non-stress conditions, whereas pigs in Groups 2, 3 and 4 were aggressively handled for 25 min to produce stress. Pigs in Groups 1, 2, 3, and 4 received intramuscular administration of saline (control group; CON), 0 (negative control group; NCON), 0.8 (low dose group; LOW), and 1.6 (high dose group; HIGH) mg rabbit liver Zn-MT per kg body weight, respectively. Pigs were slaughtered at 3 and 6 h post-injection. Zn-MT treatment increased (p<0.05) the activities of superoxide dismutase (SOD) and glutathione-peroxidase (GSH-PX) while decreasing the concentration of malondialdehyde (MDA) in liver. These responses were greater (p<0.05) at 6 h than at 3 h post Zn-MT injection. Zn-MT treatment increased (p<0.05) hepatic SOD mRNA levels in a time and dose-dependent manner and decreased (p<0.05) serum glutamate-pyruvate transaminase and lactate dehydrogenase activities (indicators of tissue integrity). Zn-MT administration decreased (p<0.05) lactate concentration and increased (p<0.05) pH and water-holding capacity in the longissimus thorasis meat. Collectively, our results indicate that intramuscular administration of Zn-MT to pre-slaughter stressed pigs improved tissue anti-oxidative ability and meat quality.
 Keywords
Stress;Metallothionein;Anti-oxidative Enzyme;Superoxide Dismutase Gene Expression;Pork Meat Quality;
 Language
English
 Cited by
1.
The Additivity of True or Apparent Phosphorus Digestibility Values in Some Feed Ingredients for Growing Pigs,;;;;;;;;;;;

아세아태평양축산학회지, 2007. vol.20. 7, pp.1092-1099 crossref(new window)
2.
Dietary Supplementation with Acanthopanax senticosus Extract Modulates Cellular and Humoral Immunity in Weaned Piglets,;;;;;;;;;;;;;

아세아태평양축산학회지, 2007. vol.20. 9, pp.1453-1461 crossref(new window)
3.
Effects of Nanocalcium Supplemented Milk on Bone Calcium Metabolism in Ovariectomized Rats,;;;;

아세아태평양축산학회지, 2007. vol.20. 8, pp.1266-1271 crossref(new window)
4.
True Digestibility of Phosphorus in Different Resources of Feed Ingredients in Growing Pigs,;;;;;;;;;;;

아세아태평양축산학회지, 2008. vol.21. 1, pp.107-119 crossref(new window)
5.
Beneficial Effects of Maternal Vitamin E Supplementation on the Antioxidant System of the Neonate Chick Brain,;;;;

아세아태평양축산학회지, 2008. vol.21. 2, pp.225-231 crossref(new window)
6.
Effect of Galacto-mannan-oligosaccharides or Chitosan Supplementation on Cytoimmunity and Humoral Immunity in Early-weaned Piglets,;;;;;;;;;;;;

아세아태평양축산학회지, 2008. vol.21. 5, pp.723-731 crossref(new window)
7.
Effects of Vitamin E Supplementation on Antioxidation and Lipid Profiles of Rats on Diets Supplemented with Cholesterol and Olive Oil,;;;

아세아태평양축산학회지, 2008. vol.21. 6, pp.883-889 crossref(new window)
8.
Meat Quality, Digestibility and Deposition of Fatty Acids in Growing-finishing Pigs Fed Restricted, Iso-energetic Amounts of Diets Containing either Beef Tallow or Sunflower Oil,;;;;;;;;;

아세아태평양축산학회지, 2008. vol.21. 7, pp.1015-1026 crossref(new window)
9.
Effects of Dietary Glycine Betaine on Growth and Pork Quality of Finishing Pigs,;;;;

아세아태평양축산학회지, 2009. vol.22. 5, pp.706-711 crossref(new window)
10.
Effects of Dietary Thiazolidinedione Supplementation on Growth Performance, Intramuscular Fat and Related Genes mRNA Abundance in the Longissimus Dorsi Muscle of Finishing Pigs,;;;;;;

아세아태평양축산학회지, 2013. vol.26. 7, pp.1012-1020 crossref(new window)
11.
Protected Organic Acid Blends as an Alternative to Antibiotics in Finishing Pigs,;;;

아세아태평양축산학회지, 2014. vol.27. 11, pp.1600-1607 crossref(new window)
1.
Co-dependence of genotype and dietary protein intake to affect expression on amino acid/peptide transporters in porcine skeletal muscle, Amino Acids, 2016, 48, 1, 75  crossref(new windwow)
2.
Effects of Dietary Isoflavone Supplementation on Meat Quality and Oxidative Stability During Storage in Lingnan Yellow Broilers, Journal of Integrative Agriculture, 2014, 13, 2, 387  crossref(new windwow)
3.
Dietary arginine supplementation enhances antioxidative capacity and improves meat quality of finishing pigs, Amino Acids, 2010, 38, 1, 95  crossref(new windwow)
4.
Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs, Molecular and Cellular Endocrinology, 2013, 365, 1, 44  crossref(new windwow)
5.
Regulation of soy isoflavones on weight gain and fat percentage: evaluation in a Chinese Guangxi minipig model, animal, 2011, 5, 12, 1903  crossref(new windwow)
6.
Effects of folic acid on the performance of suckling piglets and sows during lactation, Journal of the Science of Food and Agriculture, 2011, 91, 13, 2371  crossref(new windwow)
7.
Protected Organic Acid Blends as an Alternative to Antibiotics in Finishing Pigs, Asian-Australasian Journal of Animal Sciences, 2014, 27, 11, 1600  crossref(new windwow)
8.
Effects of Dietary Supplementation with Glutamate and Aspartate on Diquat-Induced Oxidative Stress in Piglets, PLOS ONE, 2015, 10, 4, e0122893  crossref(new windwow)
9.
Influence of nano-size reduction on absorption and bioavailability of calcium from fortified milk powder in rats, Food Research International, 2014, 66, 1  crossref(new windwow)
 References
1.
Adel, J. and N. de Ruiter. 1989. Inhibition of hydroxyl radicalgenerated DNA degradation by metallothionein. Toxicol. Lett. 47:191-196. crossref(new window)

2.
Bremner, I. 1987. Interactions between metallothiothionein and trace metals. Prog. Food Nutr. Sci. 11:1-37.

3.
Cai, L., J. B. Klein and Y. J. Kang. 2000. Metallothionein inhibits peroxynitrite-induced DNA and lipoprotei damage. J. Biol. Chem. 275:38957-38960. crossref(new window)

4.
Chen, Q. M. and L. X. Wang. 1997. Modern Pig Production. China Agricultural University Press.

5.
D'Souza, D. N., F. R.Dunshea, R. D. Warner and B. J. Leury. 1998. The effect of handingpre-slaughter and carcass processing rate post-slaughter on pork quality. Meat Sci. 50:429-437. crossref(new window)

6.
Fang, Y. Z., S. Yang and G. Wu. 2002. Free radicals, antioxidants, and nutrition. Nutrition 18:872-879. crossref(new window)

7.
Green, E. M. and H. R. Adams. 1992. New perspectives in circulatory shock: pathophysiologic mediator of the mammalian response to endotoxemia and sepsis. Am. J. Vet. Med. 200:1834-1841.

8.
Jin, H. Y., F. Q. Li and D. X. Fang. 2001. Study on the relationship of metallothionein and antioxidases in protective mechanism of cardiomyocytes. Chin. J. Appl. Physiol. 17:61-63.

9.
Kil, D. Y., S. N. Ryu, L. G. Piao, C. S. Kong, S. J. Han and Y. Y. Kim. 2006. Effect of feeding cyanidin 3-glucoside (C3G) high black rice bran on nutrient digestibility, blood measurements, growth performance and pork quality of pigs. Asian-Aust. J. Anim. Sci. 19:1790-1783.

10.
Kojima, Y. 1991. Definitions and nomenclature of metallothioneins. In: Methods in Enzymology (Ed. J. F. Riordan and B. L. Vallee). pp. 8-10.

11.
Li, Q. P., X. H. Qiao and X. D. Wang. 2003. Effect of dietary supplementation with vitamin E on pork meat quality. J. Chin. Anim. Sci. 39:34-35.

12.
NPPC. 1994. Pork chain quality audit. Progress report. National Pork Producers Council. Des Moines, IA.

13.
NRC. 1998. Nutrient Requirements of Swine, 10th Edition. National Academy Press, Washington, DC.

14.
Qi, K., X. Zhang, Q. Gao, C. Wang, X. Zhang and X. Shi. 1999. The study on metabolization of oxygen free radical about endotoxic microcycle in goat. Chin. Agric. Sci. 32:90-95.

15.
Rosenvold, K. and H. J. Andersen. 2003. Factors of significance for pork quality-a review. Meat Sci. 64:219-237. crossref(new window)

16.
SAS. 2000. SAS User's Guide. Cary, NC: SAS Institute, Inc

17.
Sato, M. and Z. Bremner. 1993. Oxygen free radicals and metallothionein. Free Radic. Biol. Med. 14:325-337. crossref(new window)

18.
Sato, M. and M. Sasaki. 1991. Enhanced lipid peroxidation is not necessary for induction of metallothionein-I by oxidative stress. Chem. Biol. Interact. 78:143-154. crossref(new window)

19.
Tamai, K. T., E. B. Gralla, L. M. Ellerby, J. S. Valentine and D. J. Thiele. 1993. Yeast and mammalian metallothioneins functionally substitute for yeast copper-zinc superoxide dismutase. Proc. Natl. Acad. Sci. USA. 90:8013-8017. crossref(new window)

20.
Tang, Z. R., Y. L. Yin, C. M. Nyachoti, R. L. Huang, T. J. Li, C. B. Yang, X. J. Yang, J. S. Gong, J. Peng, D. S. Qi, J. J. Xing, Z. H. Sun and M. Z. Fan. 2005. Effect of dietary supplementation of chitosan and galacto-mannan-oligosaccharide on serum parameters and the insulin-like growth factor-I mRNA expression in early-weaned piglets. 28:430-441. crossref(new window)

21.
Thornalley, P. J. and M. Vasak. 1985. Possible role for metallothionein in protection against radiation- induced oxidative stress kinetics and mechanism of its reaction with superoxide and hydroxyl radicals. Biochim. Biophys. Acta, 827:36-44. crossref(new window)

22.
Wu, G., Y. Z. Fang, S. Yang, J. R. Lupton and N. D. Turner. 2004. Glutathione metabolism and its implications for health. J. Nutr. 134:489-492.

23.
Yang, C. B., A. K. Li, Y. L. Yin, R. L. Huang, T. J. Li, L. L. Li, Y. P. Liao, Z. Y. Deng, J. Zhang, B. Wang, Y. G. Zhang, X. J. Yang, J. Peng and M. Z. Fan. 2005. Effects of dietary supplementation of cysteamine on growth performance, carcass quality, serum hormones and gastric ulcer in finishing pigs. J. Sci. Food Agric. 85:1947-1952. crossref(new window)

24.
Yang, Y. 1989. The mechanism and meaning of oxygen free radical produced by lick-up cells. Physiol. Sci. (Chinese). 9:10-13.

25.
Yang, J. Z., Y. Z. Xiong and H. Wang. 1990. Study on serum enzymes and halothan sensitivity in relation to porcine muscle quality. Scientia Agric. Sinica. 23:67-76.

26.
Yang, J. Z., B. P. Zhao, C. Y. Deng and Y. Z. Xiang. 1993. Pig plasma enzyme activities of glycolysis in relation to halothane genotype and meat quality. J. Huanzhong Agric. Univ. 12:478-481.

27.
Yin, Y. L., S. K. Baidoo, J. L. Boychuk and H. H. Simmins. 2001. Performance and carcass characteristics of growing pigs and broilers fed diets containing micronised barley, ground barley, wheat and maize. J. Sci. Food Agric. 81:1487-1497. crossref(new window)

28.
Zhang, J. X. and S. L. Fu. 2003. The relationship between metallothionein and oxidative stress induced by lead in liver of mice offspring. Chin. Occupational Med. 30:11-13.

29.
Zhang, J., H. Li and B. Ru. 1999. Studies on the function of metallothionein to scavenge hydroxyl radical. ACTA Scientiarum Naturalium Universitatis Pekinensis. 35:573-576.

30.
Zhou, Z. B., H. Q. Ding, F. J. Qin, L. Liu and S. Cheng. 2003. Effect of Zn-metallothionein on oxidative stress in liver of rats with severe thermal injury. Acta Pharmacologica Sinica. 24:764-770.

31.
Zhou, X. W. 2000. Animal Biochemistry. China Agricultural Press. Beijing, China.