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Improved Expression of Muscle-derived Growth Hormone Releasing Hormone from -Skeletal-muscle Actin Enhancer/Cytomegalovirus Hybrid Enhancer/Promoter
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 Title & Authors
Improved Expression of Muscle-derived Growth Hormone Releasing Hormone from -Skeletal-muscle Actin Enhancer/Cytomegalovirus Hybrid Enhancer/Promoter
Gong, Xia; Meng, Qingyong; Jin, Weiwu; Li, Ning;
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 Abstract
Growth hormone-releasing hormone (GHRH), a hypothalamic neuropeptide can stimulate the growth hormone secretion from the anterior pituitary. In this study, a porcine GHRH expression plasmid pHC-GHRH was used to enhance growth performance through ectopic expressions in muscle tissues of rats. Rats injected with the plasmid of pHC-GHRH and pCMV-GHRH exhibited cumulative weight gains 6.4% and 1% greater than controls. During a 5-day period, significant weight gain differences were observed as follows compared with that of control: during 5-10 days post-injection (DPI) period, the group pHC-GHRH on average 14.5% heavier than controls, g vs. g (p = 0.0023); during 10-15 DPI period, the group pHC-GHRH on average 13.6% heavier than controls, g vs. g (p = 0.0146); during 15-20 DPI period, the group pHC-GHRH on average 17.8% heavier than controls, g vs. g (p<0.05). In addition, plasmids-treated rats maintained higher serum IGF-I than controls. Significant differences of IGF-I were observed on 13 DPI and on 40 DPI in pHC-GHRH group compared with that of controls. This was accomplished through the use of an improved expression cassette that included the cytomegalovirus (CMV) immediate early enhancer/promoter in combination with a 1.5-kilobase portion of porcine -skeletal muscle actin promoter.
 Keywords
GHRH;Plasmid DNA;Hybrid Enhancer/Promoter;Growth;Rat;CMV;
 Language
English
 Cited by
 References
1.
Akiyama, C., S. Kobayashi and I. Nonaka. 1992. Comparison of behavior in muscle fiber regeneration after bupivacaine hydrochloride- and acid anhydride-induced myonecrosis. Acta Neuropathol. 83:584-589. crossref(new window)

2.
Carrel, A. L. and D. B. Allen. 2000. Effects of growth hormone on body composition and bone metabolism. Endocrine 12:163-172. crossref(new window)

3.
Corpas, E., S. M. Harman, M. A. Pineyro, R. Roberson and M. R. Blackman. 1993. Continuous subcutaneous infusions of growth hormone (GH) releasing hormone 1-44 for 14 days increase GH and insulin-like growth factor-I levels in old men. J. Clin. Endocrinol. Metab. 76:134-138. crossref(new window)

4.
Dai, Y., M. Roman, R. K. Naviaux and I. M. Vema. 1992. Gene therapy via primary myoblasts: long-term expression of factor IX protein following transplantation in vivo. Proc Nat. Acad. Sci. 89:10892-10895. crossref(new window)

5.
Danko, L., J. D. Fritz, S. Jiao, K. Hogan, J. S. Laendresse and J. A. Wolf. 1994. Pharmacological enhancement of in vivo foreign gene expression in muscle. Gene. Ther. 1:114-121.

6.
Davey, H. W., J. K. Kelly and A. G. Wildeman. 1995. The nucleotide sequence, structure, and preliminary studies on the transcriptional regulation of the bovine alpha skeletal actin gene. DNA Cell Biol. 14:609-618. crossref(new window)

7.
Davis, H. L., R. G. Whalen and B. A. Demeneix. 1993. Direct gene transfer into skeletal muscle in vivo: factors affecting efficiency of transfer and stability of expression. 4:151-159. crossref(new window)

8.
Draghia-Akli, R., X. G. Li and R. J. Schwartz. 1997. Enhanced growth by ectopic expression of growth hormone-releasing hormone using an injectable myogenic vector. Nat. Biotechnol. 15:1285-1289. crossref(new window)

9.
Draghia-Akli, R., M. L. Fiorotto, L. A. Hill, P. B. Malone, D. R. Deaver and R. J. Schwartz. 1999. Myogenic expression of an injectable protease-resistant growth hormone-releasing hormone augments long-term growth in pigs. Nat. Biotechnol. 17:1179-1183. crossref(new window)

10.
Etherton, T. D. and D. E. Bauman. 1998. Biology of somatotropin in growth and lactation of domestic animals. Physiol. Rev. 78:745-761.

11.
Grounds, M. D. 1991. Towards understanding skeletal muscle regeneration. Pathol. Res. Pract. 187:1-22.

12.
Kojima, M., H. Hosoda, Y. Date, M. Nakazato, H. Matsuo and K. Kangawa. 1999. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 402:656-660. crossref(new window)

13.
Lapierre, H., G. Pelletier, D. Petitclerc, P. Dubreuil, J. Morisset, P. Gaudreau, Y. Couture and P. Brazeau. 1991. Effect of human growth hormone-releasing factor and (or) thyrotropinreleasing factor on growth, carcass composition, diet digestibility, nutrient balance, and plasma constituents in dairy calves. J. Anim. Sci. 69:587-598.

14.
Mar, J. H. and C. P. Ordhal. 1990. M-CAT binding factor, a novel trans-acting factor governing muscle specific transcription. Mol. Cell Biol. 10:4271-4283.

15.
Muscat, G. E. and L. Kedes. 1987. Mutiple 5'flanking region of the human $\alpha$-skeletal actin gene synergistically modulate muscle-specific expression. Mol. Cell Biol. 7:4089-4099.

16.
Raff, T., M. Van Der Giet, D. Endemann, T. Wiederholt and M. Paul. 1997. Design and testing of beta-actin primers for RTPCR that do not co-amplify processed pseudogenes. Biotechniques 23:456-460.

17.
Reecy, J. M., C. A. Bidwell, G. P. Briley and A. L. Grant. 1996. Structure and regulation of the porcine skeletal alpha-actin encoding gene. Gene. 180:23-28. crossref(new window)

18.
Scanlon, M. F., B.G. Issa and C. Dieguez. 1996. Regulation of growth hormone secretion. Horm. Res. 46:149-154. crossref(new window)

19.
Taylor, A., H. P. Erba, G. E. Muscat and L. Kedes. 1988. Nucleotide sequence and expression of the human skeletal $\alpha$- actin gene: evolution of functional regulatory domains. Genom. 3:323-336. crossref(new window)

20.
Thorner, M. O., L. A. Frohman, D. A. Leong, J. Thominet, T. Downs, P. Hellmann, J. Chitwood, J. M. Vaughan and W. Vale. 1984. Extrahypothalamic growth-hormone-releasing factor (GRF) secretion is a rare cause of acromegaly: plasma GRF levels in 177 acromegalic patients J. Clin. Endocrinol. Metab. 59:846-849. crossref(new window)

21.
Tripathy, S. K., E. C. Svensson, H. B. Black, E. Goldwasser, M. Margalith, P. M. Hobart and J. M. Leiden. 1996. Long-term expression of erythropoietin in the systemic circulation of mice after intramuscular injection of a plasmid DNA vector. Proc. Natl. Acad. Sci. USA. 93:10876-10880. crossref(new window)

22.
Wang, J. M., H. Zheng, Y. Sugahara, J. Tan, S. N. Yao, E. Olson and K. Kurachi. 1996. Construction of human factor IX expression vectors in retroviral frames optimized for muscle cells. Hum. Gene. Ther. 7:1743-1756. crossref(new window)