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Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture
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 Title & Authors
Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture
Jeong, Dong Kee; Griswold, Michael D.;
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The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be realized if stem cells could be cultured, transfected with unique genes, and then transplanted into recipient testes. We developed a culture system that supported long-term viability of SSCs. We used a retrovirus vector (pMSCV including -galactosidase) to stably transfect spermatogonia following long-term culture using the system developed. Expression of the reporter gene -galactosidase controlled by the retroviral vector was stable in long-term cultured SSCs. We confirmed the retroviral-mediated -galactsidase gene could be expressed in germ cells in recipient mice following SSCs transplantation.
Retrovirus;Gene Transfer;Electroporation;Stem Cell Medium;
 Cited by
Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection,Kim, D.Y.;McElroy, S.L.;

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