Advanced SearchSearch Tips
Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture
Jeong, Dong Kee; Griswold, Michael D.;
  PDF(new window)
The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be realized if stem cells could be cultured, transfected with unique genes, and then transplanted into recipient testes. We developed a culture system that supported long-term viability of SSCs. We used a retrovirus vector (pMSCV including -galactosidase) to stably transfect spermatogonia following long-term culture using the system developed. Expression of the reporter gene -galactosidase controlled by the retroviral vector was stable in long-term cultured SSCs. We confirmed the retroviral-mediated -galactsidase gene could be expressed in germ cells in recipient mice following SSCs transplantation.
Retrovirus;Gene Transfer;Electroporation;Stem Cell Medium;
 Cited by
Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection,;;

아세아태평양축산학회지, 2008. vol.21. 5, pp.648-656 crossref(new window)
Avarbock, M. R., C. J. Brinster and R. L. Brinster. 1996. Reconstitution of spermatogenesis from frozen testicular germ cells. Nat. Med. 2(6):693-696. crossref(new window)

Brinster, R. L. and J. W. Zimmermann. 1994. Spermatogenesis following male germ-cell transplantation. Proc. Natl. Acad. Sci. USA. 91(24):11298-11302.

Brinster, R. L. and M. R. Avarbock. 1994. Germline transmission of donor haplotype following spermatogonial transplantation. Proc. Natl. Acad. Sci. USA., 91(24):11303-11307.

Brinster, R. L. 2002. Germline stem cell transplantation and transgenesis. Sci. 296:2174-2176. crossref(new window)

Cooker, L. A., C. D. Brooke, M. Kumari, M. C. Hofmann, J. L. Millan and E. Goldberg. 1993. Genomic structure and promoter activity of the human testis lactate dehydrogenase gene. Biol. Reprod. 48(6):1309-1319. crossref(new window)

Danno, S., K. Itoh, C. Baum, W. Ostertag, N. Ohnishi, T. Kido, K. Tomiwa, T. Matsuda and J. Fujita. 1999. Efficient gene transfer by hybrid retroviral vectors to murine spermatogenic cells. Hum. Gene. Ther. 10(11):1819-1831. crossref(new window)

Donovan, P. J. 1998. The germ cells-the mother of all stem cells. Int. J. Dev. Biol. 42:1043-1050.

Feng, Li-X., Y. Chen, L. Dettin, R. R. Pera, J. C. Herr, E. Goldberg and M. Dym. 2002 Generation and in vitro differentiation of a spermatogonial cell line. Sci. 297:392-395. crossref(new window)

Grez, M., E. Akgun, F. Hilberg and W. Ostertag. 1990. Embryonic stem cell virus, a recombinant murine retrovirus with expression in embryonic stem cells. Proc. Natl. Acad. Sci. USA. 87(23):9202-9206.

Harrison, D. E. 1980. Lifespans of immunohemopoietic stem cell lines. Adv. Pathobiol. 7:187-199.

He, M. L., W. Z. Yang, H. Hidari and W. A. Rambeck. 2006. Effect of rare earth elements on proliferation and fatty acids accumulation of 3T3-L1 cells. Asian-Aust. J. Anim. Sci. 19(1):119-125.

Hong, Y. H., Y. K. Moon, D. K. Jeong and J. Y. Han. 1998. Improved transfection efficiency of chicken gonadal primordial germ cells for the production of transgenic poultry. Transgenic. Res. 7(4):247-252.

Jeong, D. K., D. J. McLean and M. D. Griswold. 2003. Long-term culture and transplantation of murine testicular germ cells. J. Androl. 24(5):661-669.

Jeong, D. K. 2005. Study on the gene expression in stem cells of retroviral vector using electroporation method. J. Subtropical Agri. Biotech. 21(2):13-21.

Kanatsu-Shinohara, M., A. Ogura, M. Ikegawa, K. Inoue, N. Ogonuki, K. Tashiro, S. Toyokuni, T. Honjo and T. Shinohara. 2002. Adenovims-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice. Proc. Natl. Acad. Sci. USA. 99(3):1383-1388.

Lewis, P. F. and M. Emerman. 1994. Passage through mitosis is required for oncoretroviruses but not for the human immunodeficiency virus. J. Virol. 68(1):510-516.

McLaren, A. and G. Durcova-Hills. 2001. Germ cells and pluripotent stem cells in the mouse. Reprod. Fertil. 13(7-8):661-664. crossref(new window)

McLean, D. J., L. D. Russell and M. D. Griswold. 2002. Biological activity and enrichment of testicular germ cells in vitamin a-deficient and hyperthermia-exposed testes from mice based on colonization following germ cell transplantation. Biol. Reprod. 66(5):1374-1379. crossref(new window)

Miguel, M. P. and P. J. Donovan. 2003. Determinants of Retroviral-Mediated Gene Delivery to Mouse Spermatogonia. Biol. Reprod. 68(3):860-866. crossref(new window)

Mulligan, R. C. 1993. The basic science of gene therapy. Sci. 260:926-932. crossref(new window)

Muramatsu, T., O. Shibata, S. Ryoki, Y. Ohmori and J. Okumura. 1997. Foreign gene expression in the mouse testis by localized in vivo gene transfer. Biochem. Biophys. Res. Commun. 233(1):45-49. crossref(new window)

Nagano, M., M. R. Avarbock, E. B. Leonida, C. J. Brinster and R. L. Brinster. 1998. Culture of mouse testicular germ cells. Tissue Cell. 30(4):389-397. crossref(new window)

Nagano, M., T. Shinohara, M. R. Avarbock and R. L. Brinster. 2000. Retrovims-mediated gene delivery into male germ line stem cells. FEBS Lett. 475(1):7-10. crossref(new window)

Nagano, M., B. Y. Ryu, C. J. Brinster, M. R. Avarbock and R. L. Brinster. 2003. Maintenance of Mouse Male Germ Line Stem Cells In vitro. Biol. Reprod. 68(6):2207-2214. crossref(new window)

O'Brien, D. A. 1993. Isolation, separation, and short-term culture of spermatogenic cells. Methods Tox. 3A:246-264.

Ory, D. S., B. A. Neugeboren and R. C. Mulligan. 1996. A stable human-derived packaging cell line for production of high liter retrovirus/vesicular stomatitis virus G ps&idotypes. Proc. Natl. Acad. Sci. USA. 93(21):11400-11406.

Rassoulzadegan, M., V. Paquis-Flucklinger, B. Bertino, J. Sage, M. Jasin, K. Miyagawa, V. van Heyningen, P. Besmer and F. Cuzin. 1993. Transmeiotic differentiation of male germ cells in culture. Cell. 75(5):997-1006. crossref(new window)

Risley, M. S. 1990. Support of Xenopus laevis spermatogenesis in vitro by different energy substrates. Biol. Reprod. 42(3):511-522. crossref(new window)

Roe, T., T. C. Reynolds, G. Yu and P. O. Brown. 1993. Integration of murine leukemia virus DNA depends on mitosis. EMBO J. 12(5):2099-2108.

Till, J. E. and McCulloch. 1980. Hemopoietic stem cell differentiation. Bio. Chem. Biophys. Acta. 605(4):431-459.

Yamazaki, Y., H. Fujimoto, H. Ando, T. Ohyama, Y. Hirota and T. Noce. 1998. In vivo gene transfer to mouse spermatogenic cells by deoxyribonucleic acid injection into seminiferous tubules and subsequent electroporation. Biol. Reprod. 59(6): 1439-1444. crossref(new window)

Yamazaki, Y., T. Yagi, T. Ozaki and K. Imoto. 2000. In vivo gene transfer to mouse spermatogenic cells using green fluorescent protein as a marker. J. Exp. Zool. 286(2):212-218. crossref(new window)