JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Isolation and Cloning of Porcine SLC27A2 Gene and Detection of Its Polymorphism Associated with Growth and Carcass Traits
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Isolation and Cloning of Porcine SLC27A2 Gene and Detection of Its Polymorphism Associated with Growth and Carcass Traits
Wang, Tao; Liu, Chang; Xiong, Yuan-Zhu; Deng, Chang-Yan; Zuo, Bo; Xie, Hong-Tao; Xu, De-Quan;
  PDF(new window)
 Abstract
The protein encoded by SLC27A2 gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family, and it converts free long-chain fatty acids into fatty acyl-CoA esters, and thereby plays a key role in lipid biosynthesis and fatty acid degradation. In the present study, SLC27A2 located on human chromosome 15 was selected as candidate gene and we isolated and cloned partial fragments of mRNA sequence and genomic fragments of porcine SLC27A2 gene. The coding region of the gene as determined by alignments shared 90% and 82% identity with human and mouse cDNAs, respectively. Detection in LargeWhite and Meishan breeds showed that a single nucleotide polymorphism (SNP) () existed in exon 7, which caused corresponding amino acid changed for encoding. In LargeWhite pigs it encoded for Val while in Meishan pigs it encoded for Ile, so we developed the PCR-RFLP genotype method for detection of this polymorphism. Association study in 135 reference family indicated that significant correlation existed between the polymorphism and growth and carcass traits.
 Keywords
Solute Carrier Family 27;Member 2;Pig;Polymorphism;
 Language
English
 Cited by
1.
Associations of Polymorphisms in the Mx1 Gene with Immunity Traits in Large WhitexMeishan F2 Offspring,;;;;

Asian-Australasian Journal of Animal Sciences, 2007. vol.20. 11, pp.1651-1654 crossref(new window)
2.
Isolation, Expression Pattern, Polymorphism and Association Analysis of Porcine TIAF1 Gene,;;;;;;

Asian-Australasian Journal of Animal Sciences, 2009. vol.22. 3, pp.313-318 crossref(new window)
 References
1.
Andersson, L. 2003. Identification and characterization of AMPK gamma 3 mutations in the pig. Biochem. Soc. Trans. 31:232-235 crossref(new window)

2.
Berger, J., C. Truppe, H. Neumann and S. Forss-Petter. 1998. A novel relative of the very-long-chain acyl-CoA synthetase and fatty acid transporter protein genes with a distinct expression pattern. Biochem. Biophys. Res. Commun. 247:255-260. crossref(new window)

3.
Berger, J., C. Truppe, H. Neumann and S. Forss-Petter. 1998. cDNA cloning and mRNA distribution of a mouse very longchain acyl-CoA synthetase. FEBS Lett, 425:305-309. crossref(new window)

4.
Fuji, J., K. Otsu and F. Zorzto. 1991. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Sci. 253:448-451. crossref(new window)

5.
Hamilton, D. N., M. Ellis, K. D. Miller, F. K. McKeith and D. F. Parrett. 2000. The effect of the Halothane and Rendement Napole genes on carcass and meat quality characteristics of pigs. J. Anim. Sci. 78:2862-2867.

6.
Heinzer, A. K., S. Kemp, J. F. Lu, P. A. Watkins and K. D. Smith. 2002. Mouse very long-chain acyl-CoA synthetase in X-linked adrenoleukodystrophy. J. Biol. Chem. 277:28765-28773. crossref(new window)

7.
Hirsch, D., A. Stahl and H. F. Lodish. 1998. A family of fatty acid transporters conserved from mycobacterium to man. Proceedings of the National Academy of Sciences USA. 95:8625-8629.

8.
Krzecio, E., J. Kuryl, Kocwin, M. Podsiadls and G. Monin. 2005. Association of calpastatin (CAST/MspI) polymorphism with meat quality parameters of fatteners and its interaction with RYR1 genotypes. J. Anim. Breed Genet. 122:251-258. crossref(new window)

9.
Liu, B. H. 1998. Statistical Genomics: Linkage, Mapping, and QTL Analysis. CRC Press, LLC, 404-409.

10.
Mootha, V. K., J. Bunkenborg, J. V. Olsen, M. Hjerrild, J. R. Wisniewski, E. Stahl, M. S. Bolouri, H. N. Ray, S. Sihag, M. Kamal, N. Patterson, E. S. Lander and M. Mann. 2003. Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. Cell, 115:629-640. crossref(new window)

11.
Niu, B. Y., Y. Z. Xiong, S. W. Jiang, F. E. Li, C. Y. Deng, S. H. Ding, W. H. Guo, M. G. Lei, R. Zheng, B. Zuo, D. Q. Xu and J. L. Li. 2006. Oviduct-specific Glycoprotein 1 Locus is Associated with Litter Size and Weight of Ovaries in Pigs. Asian-Aust. J. Anim. Sci. 19:632-637.

12.
Schaffer, J. E. and H. F. Lodish. 1994. Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein. Cell, 79:427-436. crossref(new window)

13.
Uchiyama, A., T. Aoyama, K. Kamijo, Y. Uchida, N. Kondo, T. Orii and T. Hashimoto. 1996. Molecular cloning of cDNA encoding rat very long-chain acyl-CoA synthetase. J. Biol. Chem. 271:30360-30365. crossref(new window)

14.
Wakui, K., T. Aoyama, A. Uchiyama, T. Hashimoto and Y. Fukushima. 1998. Assignment of human fatty-acid-Coenzyme A ligase, very long-chain 1 gene (FACVL1) to human chromosome band 15q21.2 by fluorescence in situ hybridization. Cytogenetics and Cell Genetics, 81:292-293. crossref(new window)

15.
Xiong, Y. Z. and C. Y. Deng. 1999. Principle and Method of Swine Testing. Chinese Agriculture Press, Beijing (in Chinese).

16.
Zuo, B., Y. Z. Xiong, Y. H. Su, C. Y. Deng, R. Zheng and S. W. Jiang. 2003. Mapping quantitative trait loci meat quality on pig chrosomone 3, 4 and 7. Asian-Aust. J. Anim. Sci. 16:320-324.

17.
Zuo, B., Y. Z. Xiong, C. Y. Deng, Y. H. Su, J. Wang, M. G. Lei, F. E. Li, S. W. Jiang and R. Zheng. 2005. Polymorphism, linkage mapping and expression pattern of the porcine skeletal muscle glycogen synthase (GYS1) gene. Anim. Genet. 36:254-257. crossref(new window)