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Somatic Cell Nuclear Transfer of Oocytes Aspirated from Postovulatory Ovarian Follicles of Superovulated Rabbits
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 Title & Authors
Somatic Cell Nuclear Transfer of Oocytes Aspirated from Postovulatory Ovarian Follicles of Superovulated Rabbits
Shang, Jiang-Hua; Xu, Ru-Xiang; Jiang, Xiao-Dan; Zou, Yu-Xi; Qin, Ling-Sha; Cai, Ying-Qian; Yang, Zhi-Jun; Zheng, Xing; Cui, Sheng;
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The aim of this study was to evaluate if oocytes, aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration, could be efficiently used as ooplasm recipients for somatic cell nuclear transfer (SCNT). Within a common SCNT protocol, a comparison between oocytes recovered by direct aspiration (aspirated) from available ovarian follicles and oocytes flushed out from oviducts (flushed) was carried out. The results showed that maturation and enucleation rates of aspirated oocytes were 70.7% and 69.2%, significantly lower than 95.3% (p<0.01) and 83.6% (p<0.05), respectively, from flushed oocytes. However, following enucleation of matured oocytes as ooplasm recipients for SCNT, no difference was recorded in fusion and cleavage rates, as well as blastocyst development from cleaved embryos or hatching of blastocysts between aspirated and flushed groups. Additionally, some matured aspirated and flushed oocytes were also used for immediate parthenogenetic activation and the resulting embryo development was not significantly different. Results from this study show the following: i) the majority of oocytes aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration are matured and can be used directly as ooplasm recipients for SCNT; ii) the reconstructed embryos derived from these oocytes have similar in vitro developmental ability to those flushed from the oviducts.
Nuclear Transfer;Oocyte;Rabbit;Follicle;Somatic Cell;
 Cited by
Retrieval of Porcine Ovarian Follicles by Different Methods,;;;

아세아태평양축산학회지, 2008. vol.21. 3, pp.353-357 crossref(new window)
Bomsel-Helmreich, O., L. N. Huyen and I. Durand-Gasselin. 1989. Effects of varying doses of hCG on the evolution of preovulatory rabbit follicles and oocytes. Hum. Reprod. 4:636-642

Brackett, B. G., J. A. Mills and G. G. Jeitles. 1972. In vitro fertilization of rabbit ova recovered from ovarian follicles. Fertil. Steril. 23:898-909

Brophy, B., G. Smolenski, T. Wheeler, D. Wells, P. L'Huillier and G. Laible. 2003. Cloned transgenic cattle produce milk with higher levels of beta-casein and kappa-casein. Nat. Biotechnol. 21:157-162 crossref(new window)

Campbell, K. H. S., R. Alberio, I. Choi, P. Fisher, R. D. W. Kelly, J. H. Lee and W. Maalouf. 2005. Cloning: eight years after Dolly. Reprod. Domest. Anim. 40:255-414 crossref(new window)

Chen, D. Y., D. C. Wen, Y. P. Zhang, Q. Y. Sun, Z. M. Han, Z. H. Liu, P. Shi, J. S. Li, J. G. Xiangyu, L. Lian, Z. H. Kou, Y. Q. Wu, Y. C. Chen, P. Y. Wang and H. M. Zhang. 2002. Interspecies implantation and mitochondria fate of pandarabbit cloned embryos. Biol. Reprod. 67:637-642 crossref(new window)

Chen, Y., Z. X. He, A. Liu, K. Wang, W. W. Mao, J. X. Chu, Y. Lu, Z. F. Fang, Y. T. Shi and Q. Z. Yang. 2003. Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes. Cell Res. 13:251-264 crossref(new window)

Dinnyes, A., Y. P. Dai, M. Barber, L. Liu, J. Xu, P. L. Zhou and X. Z. Yang. 2001. Development of cloned embryos from adult rabbit fibroblasts effect of activation treatment and donor cell preparation. Biol. Reprod. 64:257-263 crossref(new window)

Hardarson, T., K. Lundin and L. Hamberfer. 2000. The position of the metaphase II spindle cannot be predicted by the location of the first polar body in the human oocyte. Hum. Reprod. 15:1372-1376 crossref(new window)

Harper, M. J. K. 1963. Ovulation in rabbits: the time of follicular rupture and expulsion of the eggs, in relation to injection of luteinizing hormone. J. Endocrinol. 26:307-316 crossref(new window)

Hayashi, S., Y. Noda, H. Matsumoto and T. Mori. 1987. Fertilizability of unovulated mature eggs following indomethacin administration in mice. Gamete Res. 18:291-299 crossref(new window)

Hewitson, L., T. Dominko, D. Takahashi, C. Martinovich, J. Ramalho-Santos, P. Sutovsky, J. Fanton, D. Jacob, D. Monteith, M. Neuringer, D. Battaglia, C. Simerly and G. Schatten. 1999. Unique checkpoints during the first cell cycle of fertilization after intracytoplasmic sperm injection in rhesus monkeys. Nat. Med. 5:431-433 crossref(new window)

Hong, S. B., S. J. Uhm, H. Y. Lee, C. Y. Park, M. K. Gupta, B. H. Chung, K. S. Chung and H. T. Lee. 2005. Developmental ability of bovine embryos nuclear transferred with frozenthawed or cooled donor cells. Asian-Aust. J. Anim. Sci. 18:1242-1248

Jiang, Y., T. Chen, C. L. Nan, Y. C. Ouyang, Q. Y. Sun and D. Y. Chen. 2005. In vitro culture and mtDNA fate of ibex-rabbit nuclear transfer embryos. Zygote. 13:233-240 crossref(new window)

Kobayashi, Y., K. H. Wright, R. Santulli and E. E. Wallach. 1981. Ovulation and ovum maturation in the rabbit ovary perfused in vitro. Biol. Reprod. 24:483-490 crossref(new window)

Lanza, R. P., J. B. Cibelli, F. Diaz, C. T. Moraes, P. W. Farin, C. E. Farin, C. J. Hammer, M. D. West and P. Damiani. 2000. Cloning of an endangred species (Bos gaurus) using interspecies nuclear transfer. Cloning 2:79-90 crossref(new window)

Lorenzo, P. L., P. G. Rebollar, M. J. Illera, J.C. Illera, M. Illera and J. M. Alvarino. 1996. Stimulatory effect of insulin-like growth factor I and epidermal growth factor on the maturation of rabbit oocytes in vitro. J. Reprod. Fertil. 107:109-117 crossref(new window)

Mitalipov, S. M., K. L. White, V. R. Farrar, J. Morrey and W. A. Reed. 1999. Development of nuclear transfer and parthenogenetic rabbit embryos activated with inositol 1,4,5- trisphosphate. Biol. Reprod. 60:821-827 crossref(new window)

Moon, J. H., B. C. Jee, S. Y. Ku, C. S. Suh, S. H. Kim, Y. M. Choi, J. G. Kim and S. Y. Moon. 2005. Spindle positions and their distributions in in vivo and in vitro matured mouse oocytes. Hum. Reprod. 20:2207-2210 crossref(new window)

Oh, Y. K. and B. G. Brackett. 1975. Ultrastructure of rabbit ova recovered from ovarian follicles and inseminated in vitro. Fertil. Steril. 26:665-85

Rienzi, L., F. Martinez, F. Ubaldi, F. Martinea, M. Iacobelli, M. G. Minasi, S. Ferrero, J. Tesarik and E. Greco. 2003. Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI. Hum. Reprod. 18:1289-1293 crossref(new window)

Silva, C. P., K. Kommineni, R. Oldenbourg and D. L. Keefe. 1999. The first polar body does not predict accurately the location of the metaphase II meiotic spindle in mammalian oocytes. Fertil. Steril. 71:719-721 crossref(new window)

Sun, Q. Y., L. Lai, K. W. Park, B. Kuhholzer, R. S. Prather and H. Schatten. 2001. Dynamic events are differently mediated by microfilaments, microtubules, and mitogen-activated protein kinase during porcine oocyte maturation and fertilization in vitro. Biol. Reprod. 64:879-889 crossref(new window)

Varian, N. B., R. R. Maurer and R. H. Foote. 1967. Ovarian response and cleavage rate of ova in control and FSH-primed rabbits receiving varying levels of luteinizing hormone. J. Reprod. Fertil. 13:67-73 crossref(new window)

Wakayama, T., V. Tabar, I. Rodriguez, A. C. Perry, L. Studer and P. Mombaerts. 2001. Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer. Sci. 292:740-743 crossref(new window)

Yang, C. X., Z. M. Han, D. C. Wen, Q. Y. Sun, K. Y. Zhang, L. S. Zhang, Y. Q. Wu, Z. H. Kou and D. Y. Chen. 2003. In vitro development and mitochondrial fate of macaca-rabbit cloned embryos. Mol. Reprod. Dev. 65:396-401 crossref(new window)

Yin, X. J., Y. Kato and Y. Tsunoda. 2001. Effect of enucleation procedures and maturation conditions on the development of nuclear-transferred rabbit oocytes receiving male fibroblast cell. Reprod. 124:41-47