Advanced SearchSearch Tips
In vitro Development of Interspecies Somatic Cell Nuclear Transfer Embryos Derived from Murine Embryonic Fibroblasts and Bovine Oocytes
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
In vitro Development of Interspecies Somatic Cell Nuclear Transfer Embryos Derived from Murine Embryonic Fibroblasts and Bovine Oocytes
Yun, J.I.; Koo, B.S.; Yun, S.W.; Lee, Chang-Kyu;
  PDF(new window)
Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studies of iSCNT using murine cells and bovine oocytes have been reported, the development of murine-bovine iSCNT embryos beyond the 8-cell stage has not been successful. In this paper, we examined the developmental potential of embryos reconstructed with a murine embryonic fibroblast as the nuclear donor and a bovine oocyte as the cytoplasm recipient. The reconstructed embryos were cultured in CZB (murine medium) or CR1aa (bovine medium). In addition, for the development of a murine-bovine iSCNT blastocyst, the antioxidant -mercaptoethanol () was supplemented to CR1aa medium. Furthermore, to verify the mouse genome activation in murine-bovine iSCNT embryos, RT-PCR analysis of murine Xist was performed. The development of the murine-bovine iSCNT embryos cultured in CR1aa was significantly higher than that in CZB (p<0.05). With respect to the effect of BME on the development of the murine-bovine iSCNT blastocyst, addition of BME produced a significant increase in blastocyst development (p<0.05). Karyotype analysis confirmed that the reconstructed embryos were derived from murine cells (40XX). The Xist gene was gradually increased from the 8-cell stage to the blastocyst stage. This is the first report of blastocyst development of iSCNT embryos derived from murine somatic cells and bovine oocytes. These results demonstrate that bovine cytoplasm can support the development of later stages of a preimplantation embryo from murine-bovine iSCNT.
Murine;Bovine;Interspecies Somatic Cell Nuclear Transfer;-Mercaptoethanol;Xist;
 Cited by
Establishment and Characterization of the Fibroblast Line from Silkie Bantam,;;;;;

아세아태평양축산학회지, 2009. vol.22. 4, pp.492-499 crossref(new window)
Establishment and characterization of a fibroblast cell line derived from Texel sheep, Biochemistry and Cell Biology, 2009, 87, 3, 485  crossref(new windwow)
Arat, S., S. J. Rzucidlo and S. L. Stice. 2003. Gene expression and in vitro development of inter-species nuclear transfer embryos. Mol. Reprod. Dev. 66:334-342. crossref(new window)

Bagis, H. and H. Odaman Mercan. 2004. Effect of chemically defined culture medium supplemented with ${\beta}$-mercaptoethanol and amino acids on implantation and development of different stage in vivo- or in vitro-derived mouse embryos. Mol. Reprod. Dev. 69:52-59. crossref(new window)

Baguisi, A., E. Behboodi, D. T. Melican, J. S. Pollock, M. M. Destrempes, C. Cammuso, J. L. Williams, S. D. Nims, C. A. Porter, P. Midura, M. J. Palacios, S. L. Ayres, R. S. Denniston, M. L. Hayes, C. A. Ziomek, H. M. Meade, R. A. Godke, W. G. Gavin, Y. Overstrom and Y. Echelard. 1999. Production of goats by somatic cell nuclear transfer. Nat. Biotechnol. 17:456-461. crossref(new window)

Biggers, J. D., L. K. McGinnis and M. Raffin. 2000. Aminoacids and preimplantation development of the mouse in protein-free potassium simplex optimized medium. Biol. Reprod. 63:281-293. crossref(new window)

Boiani, M., S. Eckardt, H. R. Scholer and K. J. McLaughlin. 2002. Oct4 distribution and level in mouse clones: Consequences for pluripotency. Genes Dev. 16:1209-1219. crossref(new window)

Caamano, J. N., Z. A. Ryoo, J. A. Thomas and C. R. Youngs. 1996. ${\beta}$-mercaptoethanol enhances blastocyst formation rate of bovine in vitro-matured/in vitro fertilized embryos. Bio. Reprod. 55:1179-1184. crossref(new window)

Chen, D. Y., Q. Y. Sun, G. P. Liu, G. P. Li, L. Lian, M. K. Wang, Z. M. Han, X. F. Song, J. S. Li, Q. Sun, Y. C. Chen, Y. P. Chang and B. Ding. 1999. The giant panda (Ailuroda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development the reconstructed egg. Sci. China (Series C) 29:324-330.

Chesne, P., P. G. Adenot, C. Viglietta, M. Baratte, L. Boulanger and J. P. Renard. 2002. Cloned rabbits produced by nuclear transfer from adult somatic cells. Nat. Biotechnol. 20:366-369. crossref(new window)

Constance, H. 2003. First cloned mule races to finish line. Sci. 300:1354.

Daniels, R., S. Lowell, V. Bolton and M. Monk. 1997. Transcription of tissue-specific genes in human preimplantation embryos. Human Reprod. 12:2251-2256. crossref(new window)

Daniels, R., V. Hall and A. O. Trounson. 2000. Analysis of gene transcription in bovine nuclear transfer embryos reconstructed with granulosa cell nuclei. Biol. Reprod. 63:1034-1040. crossref(new window)

De La Fuente, R., A. Hahnel, P. R. Rasrur and W. A. King. 1999. X inactive-specific transcript (Xist) expression and X chromosome inactivation in the preattachment bovine embryo. Biol. Reprod. 60:769-775. crossref(new window)

Dindot, S. V., P. W. Farin, C. E. Rarin, J. Romano, S. Walker, C. Long and J. A. Piedrahita. 2004. Epigenetic and genomic imprinting analysis in nuclear transfer derived Bos gaurus/Bos Taurus hybrid fetuses. Biol. Reprod. 71:470-478. crossref(new window)

Dominko, T., M. Mitalipova, B. Haley, Z. Beyhan, E. Memili, B. Mckusick and N. L. First. 1999. Bovine oocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species. Biol. Reprod. 60:1496-1502. crossref(new window)

Galli, C., I. Laguina, G. Crotti, S. Colleoni, P. Turini, N. Ponderato, R. Duchi and G. Lazzari. 2003. A cloned horse born to its dam twin. Nature 424:635.

Kato, Y., T. Tani, Y. Sotomaru, K. Kurokawa, J. Kato, H. Doguchi, H. Yasue and Y. Tsunoda. 1998. Eight calves cloned from somatic cells of a single adult. Sci. 282:2095-2098. crossref(new window)

Kay, G. F., G. D. Penny, D. Patel, A. Ashworth, N. Brockdorff and S. Rastan. 1993. Expression of Xist during mouse development suggests a role in the initiation of X Chromosome inactivation. Cell 72:171-182. crossref(new window)

Kay, G. F., S. C. Barton, M. A. Surani and S. Rastan. 1994. Imprinting and X chromosome counting mechanisms determine Xist expression in early mouse development. Cell 77:639-650. crossref(new window)

Kenneth, L. 2001. Can cloning wave endangered species?. Curr. Biol. 11:245-246. crossref(new window)

Kin, M. K., G. Jang, H. J. Oh, F. Yuda, H. J. Kim, W. S. Whang, M. S. Hossein, J. J. Kim, N. S. Shin, S. K. Kang and B. C. Lee. 2007. Endangered wolves cloned from adult somatic cells. Cloning Stem Cells 9:130-137. crossref(new window)

Kitiyanant, Y., J. Saikhun, B. Chaisalee, K. L. White and K. Pavasuthipaisit. 2001. Somatic cell cloning in buffalo (Bubalus bubalis): Effects of interspecies cytoplasmic recipients and activation procedures. Cloning Stem Cells 3:97-104. crossref(new window)

Laibrfied-Retledge, M. L., T. Dominko, E. S. Critser and J. K. Crister. 1999. Tissue maturation in vivo and in vitro: Gamete and early embryos ontogeny, In: Reproductive tissue banking (Ed. A. M. Karow and J. M. Crister). New York Press 22-137.

Lanza, R. P., J. B. Cibelli, F. Diaz, C. T. Moraes, P. W. Farin, C. E. Farin, C. J. Hammer, M. D. West and P. Damiani. 2000. Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer. Cloning 2:79-90. crossref(new window)

Loi, P., G. Ptak, B. Barboni, J. Fulka, P. Cappai and M. Clinton. 2001. Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. Nat. Biotechnol. 19:962-964. crossref(new window)

Maurizio, Z. and B. Michele. 2002. Mouse xist express begins at zygotic genome activation and timed by z zygotic clock. Mol. Rep. Dev. 61:14-20. crossref(new window)

Niemann, H., C. Wrenzycki, A. Lucas-Hahn, T. Brambrink, W. A. Kues and J. W. Carnwath. 2002. Gene expression patterns in bovine in vitro produced and nuclear transfer-derived embryos and their implications for early development. Clon. Stem Cell 4:29-38. crossref(new window)

Noda, Y., H. Matsumoto, Y. Umaoka, K. Tatsumi and T. Mori. 1991. Improvement of superoxide radicals in mouse two-cell block phenomenon. Mol. Reprod. Dev. 28:356-360. crossref(new window)

Oguara, A., K. Inoue, K. Takano, T. Wakayama and R. Yanagimachi. 2000. Birth of mice after nuclear transfer by electrofusion using tail tip cells. Mol. Reprod. Dev. 57:55-59. crossref(new window)

Pabon, J. E. J., W. E. Findley and W. E. Gibbons. 1989. The toxic effect of short exposure to the atmospheric oxygen concentration on early mouse embryonic development. Fertil. Steril. 51:896-900.

Park, S. H., M. R. Shin and N. H. Kim. 2004. Bovine oocyte cytoplasm supports nuclear remodeling but not reprogramming of murine fibroblast cells. Mol. Reprod. Dev. 68:25-34. crossref(new window)

Park, E. S., W. S. Hwang, S. K. Kang, B. C. Lee, J. Y. Han and J. M. Lim. 2004. Improved embryo development with decreased apoptosis in blastomeres after the treatment of cloned bovine embryos with ${\beta}$-mercaptoethanol and Hemoglobin. Mol. Reprod. Dev. 67:200-206. crossref(new window)

Polejaeva, I. A., S. H. Chen, T. D. Vaught, R. L. Page, J. Mullins, S. Ball, Y. Dai, J. Boone, S. Walker, D. L. Ayares, A. Colman and K. H. Campbell. 2000. Cloned pigs produced by nuclear transferring adult somatic cells. Nature 407:86-90. crossref(new window)

Quinn, P. and G. M. Harlow. 1978. The effect of oxygen on the development of preimplantation mouse embryos in vitro. J. Exp. Zool. 206:73-80. crossref(new window)

Rideout, W. M., K. Eggan and R. Jaenish. 2001. Nuclear cloning and epigenetic reprogramming of the genome. Sci. 293:1093-1098. crossref(new window)

Rosenkrans, C. F., G. Q. Zeng, G. T. McNamara, P. K. Schoff and N. L. First. 1993. Development of bovine embryos in vitro is affected by energy substrate. Biol. Reprod. 49:459-462. crossref(new window)

Sansinena, M. J., D. Hylan, K. Hebert, R. S. Denniston and R. A. Godke. 2005. Banteng (Bos javanicus) embryos and pregnancies produced by interspecies nuclear transfer. Theriogenol. 63:1081-1091. crossref(new window)

Shin, T., D. Kraemer, J. Pryor, L. Liu, J. Rugila, L. Howe, S. Buck, K. Murphy, L. Lyons and M. Westhusin. 2002. A cat cloned by nuclear transplantation. Nature 415:859. crossref(new window)

Relford, N.A., A. J. Watson and G. A. Schulz. 1990. Transition from the maternal to embryonic control in early mammalian development: a comparison of several species. Mol. Reprod. Dev. 26:90-100. crossref(new window)

Takahashi, M., T. Nagai, S. Hamano, M. Kuwayama, N. Okamura and A. Okano. 1993. Effect of thiol compounds on in vitro development and intracellular glutathione content of bovine embryos. Biol. Reprod. 49:228-232. crossref(new window)

Umaoka, Y., Y. Noda, K. Narimoto and T. Mori. 1992. Effects of oxygentoxicity on early development of mouse embryos. Mol. Reprod. Dev. 31:28-33. crossref(new window)

Wakayama, T., A. C. Perry, M. Zuccotti, K. R. Johnson and R. Yanagimachi. 1998. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374. crossref(new window)

White, K. L., T. D. Bunch, S. Mitalipov and W. A. Reed. 1999. Establishment of pregnancy after the transfer of nuclear transfer embryos produced from the fusion of Argali (Ovis ammon) nuclei into domestic sheep (Ovis aries) enucleated oocytes. Cloning 1:47-54. crossref(new window)

Wilmut, I., A. E. Schnieke, J. McWhir, A. J. Kind and K. H. S. Campbell. 1997. Viable offspring derived from fet al and adult mammalian cells. Nature 385:810-813. crossref(new window)

Winger, Q., J. A. Hill, T. Shin, A. J. Watson, D. C. Kraemer and M. E. Westhusin. 2000. Genetic reprogramming of lactate dehydrogenase, citrate synthase and phosphofructokinase mRNA in bovine nuclear transfer embryos produced using bovine fibroblast cell nuclei. Mol. Reprod.Dev. 56:458-464. crossref(new window)

Yanxin, L., D. Yunping, D. Weihua, Z. Chunjiang, W. Haiping, W. Lili, L. Rong, L. Ying, W. Rong and L. Ning. 2006. Cloned endangered species takin (Budorcas taxicolor) by interspecies nuclear transfer and comparison of the blastocyst development with yak (Bos grunnuens) and bovine. Mol. Reprod. Dev. 73:189-195. crossref(new window)