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mtDNA Diversity and Origin of Chinese Mongolian Horses
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 Title & Authors
mtDNA Diversity and Origin of Chinese Mongolian Horses
Li, Jinlian; Shi, Youfei; Fan, Caiyun; Manglai, Dugarjaviin;
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 Abstract
In order to learn the origin of the Chinese Mongolian horse, we analyzed polymorphisms within the mtDNA D-loop variable region in 305 horses of 6 types of 3 different breeds, including one imported breed, one cultivated breed and 4 types of one local breed. We detected 13 different haplotypes, and subsequent sequence analysis showed that all 6 horse types were genetically diverse. By constructing a cladogram of mtDNA D-loop sequences from the 6 horse types along with homologous sequences from several other horse types obtained from GenBank, we showed that Chinese Mongolian horses have a close genetic relationship with other horse types from Mongolia. We also speculate that several Chinese Mongolian horses descended from Przewalskii horse. Additionally, the 13 haplotypes were dispersed throughout the cladogram, suggesting that Chinese Mongolian horses likely originated from multiple female ancestors. A phylogenetic map of the 6 horse types showed that the genetic relationship between the local Wuzhumuqin and Wushen types were the closest. The Xinihe and Baerhu were also closely related to each other, and slightly more distantly related to the cultivated Sanhe breed. All five of the local Chinese horse types had a much more distant relationship with the imported Thoroughbred breed.
 Keywords
Horses;mtDNA D-Loop Region;PCR-SSCP;Polymorphism;Origin Evolution;
 Language
English
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 References
1.
Cho, G. J. 2006. Genetic relationship among the Korean native and alien horses estimated by microsatellite polymorphism. Asian-Aust. J. Anim. Sci. 19:784-788.

2.
Cozzi, M. C., M. G. Strillacci, P. Valiati, B. Bighignoli, M. Cancedda and M. Zanotti. 2004. Mitochondrial D-loop sequence variation among Italian horse types. Genet. Sel. Evol. 36:663-672. crossref(new window)

3.
Hill, E. W., D. G. Bradley, M. Al-Barody, O. Ertugrul, R. K. Splan, I. Zakharov and E. P. Cunningham. 2002. History and integrity of thoroughbred dam lines revealed in equine mtDNA variation. Anim. Genet. 33:287-294. crossref(new window)

4.
Ishida, N., T. Hasegawa, K. Takeda, M. Sakagami, A. Onishi, S. Inumaru, M. Komatsu and H. Mukoyama. 1994. Polymorphic sequence in the D-loop region of equine mitochondrial DNA. Anim. Genet. 25:215-221. crossref(new window)

5.
Kavar, T., F. Habe, G. Brem and P. Dovc. 1999. Mitochondrial Dloop sequence variation among the 16 maternal lines of the Lipizzan horse breed. Anim. Genet. 30:423-430. crossref(new window)

6.
Kim, K. I., Y. H. Yang, S. S. Lee, C. Park, R. Ma, J. L. Bouzat and H. A. Lewin. 1999. Phylogenetic relationships of Cheju horses to other horse breeds as determined by mtDNA D-loop sequence polymorphism. Anim. Genet. 30:102-108. crossref(new window)

7.
Lopes, M. S., D. Mendonca, T. Cymbron, M. Valera, J. Costa-Ferreira and C. Machado Ada. 2005. The Lusitano horse maternal lineage based on mitochondrial D-loop sequence variation. Anim. Genet. 36:196-202. crossref(new window)

8.
Mang, L. 2002. Mongolian man and horse. Inner Mongolia Science and Technology Press. pp. 3-31.

9.
Mang, L. 2005. Wuzhumuqin horse-outstanding species on Xilingoluo grasslands. China-Collection Industry 1:77-80.

10.
Marklund, S., R. Chaudhary, L. Marklund, K. Sandberg and L. Andersson. 1995. Extensive mtDNA diversity in horses revealed by PCR-SSCP analysis. Anim. Genet. 26:193-196. crossref(new window)

11.
McGahern, A. M., C. J. Edwards, M. A. Bower, A. Heffernan, S. D. Park, P. O. Brophy, D. G. Bradley, D. E. MacHugh and E. W. Hill. 2006. Mitochondrial DNA sequence diversity in extant Irish horse populations and in ancient horses. Anim. Genet. 37:498-502. crossref(new window)

12.
McGahern, A. M., M. A. Bower, C. J. Edwards, P. O. Brophy, G. Sulimova, I. Zakharov, M. Vizuete-Forster, M. Levine, S. Li, D. E. MacHugh and E. W. Hill. 2006. Evidence for biogeographic patterning of mitochondrial DNA sequences in Eastern horse populations. Anim. Genet. 37:494-497. crossref(new window)

13.
Mirol, P. M., P. Peral Garcia, J. L. Vega-Pla and F. N. Dulout. 2002. Phylogenetic relationships of Argentinean Creole horses and other South American and Spanish breeds inferred from mitochondrial DNA sequences. Anim. Genet. 33:356-363. crossref(new window)

14.
Myka, J. L., T. L. Lear, M. L. Houck, O. A. Ryder and E. Bailey. 2003. FISH analysis comparing genome organization in the domestic horse (Equus caballus) to that of the Mongolian wild horse (E.przewalskii). Cytogenet Genome Res. 102:222-225. crossref(new window)

15.
Sambrook, J. 1992. Molecular cloning a laboratory manual (2nd ed). Cold Spring Harbor Laboratory Press. 463-481.

16.
Vila, C., J. A. Leonard, A. Gotherstrom, S. Marklund, K. Sandberg, K. Liden, R. K. Wayne and H. Ellegren. 2001. Widespread origins of domestic horse lineages. Sci. 291:474-477. crossref(new window)

17.
Wallner, B., G. Brem, M. Muller and R. Achmann. 2003. Fixed nucleotide differences on the Y chromosome indicate clear divergence between Equus przewalskii and Equus caballus. Anim. Genet. 34:453-456. crossref(new window)

18.
Wang, W., A. H. Liu, S. Y. Lin, H. Lan, B. Su, D. W. Xie and L. M. Shi. 1994. Multiple genotypes of mitochondrial DNA with a horse population from a small region in Yunnan province of China. Biochem. Genet. 32:371-378. crossref(new window)

19.
Xu, X. and U. Arnason. 1994. The complete mitochondrial DNA sequence of the horse, Equus caballus: extensive heteroplasmy of the control region. Gene. 148:357-362. crossref(new window)

20.
Zhang, Y. J. 2000 Molecular genetics. Cold Spring Harbor Laboratory Press. 442.