JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effects of Monensin and Fish Oil on Conjugated Linoleic Acid Production by Rumen Microbes in Holstein Cows Fed Diets Supplemented with Soybean Oil and Sodium Bicarbonate
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Monensin and Fish Oil on Conjugated Linoleic Acid Production by Rumen Microbes in Holstein Cows Fed Diets Supplemented with Soybean Oil and Sodium Bicarbonate
Jin, G.L.; Choi, S.H.; Lee, H.G.; Kim, Y.J.; Song, Man K.;
  PDF(new window)
 Abstract
The present study was conducted with four ruminally canulated Holstein cows to observe the effects of monensin or fish oil on diet fermentation and production of conjugated linoleic acids (CLAs) in the rumen when fed diets supplemented with soybean oil and sodium bicarbonate. Cows of the control treatment were fed a basal diet (CON) consisting of 60% commercial concentrate and 40% chopped rye grass hay. Cows of other treatments were fed the same diet as CON, but the concentrate was supplemented with 7% of soybean oil and 0.5% of sodium bicarbonate (SO-B), SO-B supplemented with monensin (30 ppm, SO-BM) or concentrate supplemented with 6.3% of soybean oil, 0.5% of sodium-bicarbonate, 30 ppm of monensin and 0.7% of fish oil (SO-BMF). Dry matter (DM) intake of the cows was significantly (p<0.011) reduced by feeding the SO-BMF diet compared to the other diets which did not differ in DM intake. Whole tract digestibility of major dietary components was significantly (p<0.004-0.027) higher for SO-BMF than the other supplement-containing diets. Dietary supplements did not clearly affect rumen pH and ammonia concentrations compared to the CON diet. Significantly reduced (p<0.05) total VFA concentration was obtained by the addition of fish oil to the diet (SO-BMF) compared to other diets. No differences, however, were obtained in major VFA proportions as well as in total VFA between the supplemented diets. The SO-BM diet increased (p<0.01-0.05) the concentrations of trans-11 and linoleic acid in rumen fluid. Total CLA concentration was also increased by the feeding of SO-B and SO-BM diets during early fermentation times (up to 3 h) post-feeding. Cis-9, trans-11 CLA concentration in rumen fluid was highest (p<0.05) for SO-B up to 1 h while the highest (p<0.01) value for SO-BM occurred at 3 h post-feeding. An increased trans-10, cis-12 CLA concentration was obtained from the SO-B and SO-BM diets at 1 and 3 h post feeding compared to the other diets. Supplementation of oils with monensin and sodium bicarbonate increased (p<0.05) the proportions of and CLA in the plasma of cows, but the effect of monensin and/or fish oil was limited to trans-10, cis-12 CLA.
 Keywords
Monensin;Fish Oil;Soybean Oil;Buffer;CLA;Fatty Acid Composition;Biohydrogenation;Rumen Microbes;Plasma;Holstein Cows;
 Language
English
 Cited by
1.
Conjugated Linoleic Acid in Rumen Fluid and Milk Fat, and Methane Emission of Lactating Goats Fed a Soybean Oil-based Diet Supplemented with Sodium Bicarbonate and Monensin,;;;;;;;;;

아세아태평양축산학회지, 2009. vol.22. 11, pp.1521-1530 crossref(new window)
2.
Linolenic Acid in Association with Malate or Fumarate Increased CLA Production and Reduced Methane Generation by Rumen Microbes,;;;;;;;

아세아태평양축산학회지, 2009. vol.22. 6, pp.819-826 crossref(new window)
3.
The Effect of Forage Level and Oil Supplement on Butyrivibrio fibrisolvens and Anaerovibrio lipolytica in Continuous Culture Fermenters,;;;

아세아태평양축산학회지, 2012. vol.25. 2, pp.234-239 crossref(new window)
 References
1.
Albright, C. D., E. Klem, A. A. Shah and P. Gallagher. 2005. Breast cancer cell targeted oxidative stress: Enhancement of cancer uptake of conjugated linoleic acid, activation of p53, and inhibition of proliferation. Exp. Molec. Pathol. 79:118-125. crossref(new window)

2.
AOAC. 1995. Official methods of analysis (15th ed.). Association of Official Analytical Chemists, Washington, DC.

3.
Ashes, J. R., B. D. Siebert, S. K. Gulati, A. Z. Cuthbertson and T. W. Scott. 1992. Incorporation of n-3 fatty acids of fish oil into tissue and serum lipids of ruminants. Lipids 27:629-631. crossref(new window)

4.
Bell, J. A. and J. J. Kennelly. 2000. Producing conjugated fatty acid enriched milk through practical dairy nutrition. Conference abstract:8th World Congress on Clinical Nutrition, Bankok, Thailand.

5.
Choi, N. J., J. Y. Imm, S. J. Oh, B. C. Kim, H. J. Hwang and Y. J. Kim. 2005. Effect of pH and oxygen on conjugated linoleic acid (CLA) production by mixed rumen bacteria from cows fed high concentrate and high forage diets. Anim. Feed Sci. Technol. 123-124:643-653. crossref(new window)

6.
Choi, S. H., K. W. Lim, H. G. Lee, Y. J. Kim and M. K. Song. 2007. Supplementation effects of $C_{18:2}\;or\;C_{18:3}$ rich oils on formations of CLA and TVA, and lipogenesis in adipose tissues of sheep. Asian-Aust. J. Anim. Sci. 20:1417-1423.

7.
Chouinard, P. Y., L. Corneau, M. L. Kelly, J. M. Griinari and D. E. Bauman. 1998. Effect of dietary manipulation on milk conjugated linoleic acid concentrations. J. Dairy Sci. 81(Suppl. 1):233(Abstr.).

8.
Chung, T. Y., M. K. Song, C. S. Moon, C. M. Kim, D. I. Kim and J. S. Sim. 1996. Effect of flaxseed supplementation on the nutrient utilizing efficiency, milk productivity and fatty acid composition of milk by lactating Holstein cows. Kor. J. Nutr. Feed. 20:443-452.

9.
Cook, M. E., C. C. Miller, Y. Park and M. Pariza. 1993. Immune modulation by altered nutrient metabolism: Nutritional control of immune induced growth depression. Poult. Sci. 72:1301-1305.

10.
Dhiman, T. R., G. R. Anand, L. D. Satter and M. W. Pariza. 1996. Conjugated linoleic acid content of milk from cows fed different diets. J. Dairy Sci. 79(Suppl. 1):137(Abstr.)

11.
Dhiman, T. R., L. D. Satter, M. W. Pariza, M. P. Galli, K. Albright and M. X. Tolasa. 2000. Conjugated linoleic acid (CLA) content of milk from cows offered diets rich in linoleic and linolenic acid. J. Dairy Sci. 83:1016-1027. crossref(new window)

12.
Fawcett, J. K. and J. E. Scott. 1960. A rapid and precise method for the determination of urea. J. Clin. Pathol. 13:156-163. crossref(new window)

13.
Fellner, V., F. D. Sauer and J. K. Kramer, 1997. Effect of nigericin, monensin, and tetranasin on biohydrogenation in continuous flow-through ruminal fermenters. J. Dairy Sci. 80:921-928. crossref(new window)

14.
Grummer, R. R. 1991. Effect of feed on the composition of milk fat. J. Dairy Sci. 74:3244-3251. crossref(new window)

15.
Kobayashi, Y., M. Wakita and S. Hoshino. 1992. Effects of the ionophore salinomycin on nitrogen and long-chain fatty acid profiles of digesta in the rumen and the duodenum of sheep. Anim. Feed Sci. Technol. 36:670 crossref(new window)

16.
Lee, K. N., D. Kritchevsky and M. W. Pariza. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108:19-25. crossref(new window)

17.
Lepage, G. and C. C. Roy. 1986. Direct transesterification of all classes of lipid in a one-step reation. J. Lipid Res. 27:114-121.

18.
Loor, J. J., K. Ueda, A. Ferlay, Y. Chilliard and M. Doreau. 2005. Intestinal flow and digestibility of trans fatty acids and conjugated linoleic acids (CLA) in dairy cows fed a highconcentrate diet supplemented with fish oil, linseed oil, or sunflower oil. Anim. Feed Sci. Technol. 119:203-225. crossref(new window)

19.
SAS. 1985. SAS User's Guide: Statistical Analysis Systems Institute, Inc., Cary, NC.

20.
Sauer, F. D., V. Fellner, R. Kinsman, J. K. G. Kramer, H. A. Jackson, A. J. Lee and S. Chen. 1998. Methane output and lactation response in Holstein cattle with monensin or unsaturated fat added to the diet. J. Anim. Sci. 76:906-914.

21.
Steel, R. G. D. and J. H. Torrie. 1980. Principles and procedures of statistics. Mcgraw Hill Book Co., NY.

22.
The British Nutrition Foundation 1992. Sources of unsaturated fatty acids in the diet. In: The report of the British Nutrition Foundation's task force. Unsaturated fatty acids: nutritional and physiological significance, London, UK: Chapman and Hall, pp. 6-11.

23.
Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fibre, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597. crossref(new window)

24.
Wang, J. H. and M. K. Song. 2001. Effect od sources and levels of carbohydrates on fermentation characteristics and hydrogenation of linoleic acid by rumen bacteria in vitro. Asian-Aust. J. Anim. Sci. 14:48-53.

25.
Wang, J. H. and M. K. Song. 2003. pH affects the in vitro formation of cis-9, trans-11 CLA and trans-11 Octadecenoic acid by ruminal bacteria when incubated with oilseeds. Asian-Aust. J. Anim. Sci. 16:1743-1748.

26.
Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002. Effect of concentrate level on the formation of conjugated linoleic acid and trans-octadecenoic acid by ruminal bacteria when incubated with oilseed in vitro. Asian-Aust. J. Anim. Sci. 15:1115-1120.

27.
Wang, J. H., S. H. Choi, C. G. Yan and M. K. Song. 2005. Effect of monensin and fish oil supplementation on biohydrogenation and CLA production by rumen bacteria in vitro when incubated with safflower oil. Asian-Aust. J. Anim. Sci. 18:221-225.

28.
Wang, J. H., S. H. Choi, K. W. Lim, K. H. Kim and M. K. Song. 2006. Effect of mixed oil and monensin supplementation, and feeding duration of supplements on c9,t11-CLA contents in plasma and fat tissues of Korean native (Hanwoo) steers. Asian-Aust. J. Anim. Sci. 19:1464-1469.

29.
Whigham, L. D., A. Higbee, D. E. Bjorling, Y. H. Park, M. W. Pariza and M. E. Cook. 2002. Decreased antigen-induced eicosanoid release in conjugated linoleic acid-fed guinea pigs. Am. J. Physiol. Intergrative Comp. Physiol. 282(4):R1104-R1112.