JOURNAL BROWSE
Search
Advanced SearchSearch Tips
The Expression of Genes Related to Egg Production in the Liver of Taiwan Country Chickens
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
The Expression of Genes Related to Egg Production in the Liver of Taiwan Country Chickens
Ding, S.T.; Ko, Y.H.; Ou, B.R.; Wang, P.H.; Chen, C.L.; Huang, M.C.; Lee, Y.P.; Lin, E.C.; Chen, C.F.; Lin, H.W.; Cheng, Winston Teng Kuei;
  PDF(new window)
 Abstract
The purpose of this study was to detect expression of genes related to egg production in Taiwan Country chickens by suppression subtractive hybridization. Liver samples of mRNA extraction from two Taiwan Country chicken strains (L2 and B), originated from the same population but with very distinct egg production rates after long-term selection for egg and meat production respectively. Two-way subtraction was performed. The hepatic cDNA from the low egg production chickens (B) was subtracted from the hepatic cDNA from the high egg production strain (L2). The reversed subtraction (L2 from B) was also performed. The resulting differentially expressed gene fragments were cloned and sequenced. We sequenced 288 clones from the forward subtraction and 96 clones from the reverse subtraction. These genes were subjected to further screening to confirm the differential expression between the two genetic breeds of chickens. The apolipoprotein B (apoB) was expressed to a greater extent in the liver of the L2 than in the B line chickens. The 5-aminoimidazole- 4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (PURH) was expressed to a greater extent in the liver of the B than in the L2 strain chickens. We demonstrated that both apoB and PURH were more highly expressed in the liver than that in other tissues (muscle, ovary, and oviduct) in laying Taiwan Country chickens. Taken together, these data suggest that after the selection for egg production, expression of apoB and PURH genes were also changed. Whether the changed expression of these genes is directly related to egg production is not known, but these two genes may be useful markers for egg laying performance in Taiwan Country chickens.
 Keywords
apoB;Egg production;Liver;PURH;Taiwan Country Chicken;
 Language
English
 Cited by
1.
Identification of Differentially Expressed Proteins at Four Growing Stages in Chicken Liver,;;;;;;

Asian-Australasian Journal of Animal Sciences, 2008. vol.21. 10, pp.1383-1388 crossref(new window)
2.
Canonical Correlation Analysis for Estimation of Relationships between Sexual Maturity and Egg Production Traits upon Availability of Nutrients in Pullets,;;;

Asian-Australasian Journal of Animal Sciences, 2008. vol.21. 11, pp.1576-1584 crossref(new window)
 References
1.
Abdrakhmanov, I., D. Lodygin, P. Geroth, H. Arakawa, A. Law, J. Plachy, B. Korn and J. M. Buerstedde. 2000. A large database of chicken bursal ESTs as a resource for the analysis of vertebrate gene function. Genome Res. 10:2062-2069. crossref(new window)

2.
Aimi, J., H. Qiu, J. Williams, H. Zalkin and J.E. Dixon. 1990. De novo purine nucleotide synthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetaseglycinamide ribonucleotide transformylase by functional complementation in E. coli. Nucleic Acids Res. 18:6665-6672. crossref(new window)

3.
Baker, M. E. 1988. Is vitellogenin an ancestor of apolipoprotein B- 100 of human low-density lipoprotein and human lipoprotein lipase? Biochem. J. 255:1057-1060.

4.
Boren, J., I. Lee, W. Zhu, K. Arnold, S. Taylor and T. L. Innerarity. 1998. Identification of the low density lipoprotein receptorbinding site in apolipoprotein B100 and the modulation of its binding activity by the carboxyl terminus in familial defective apo-B100. J. Clin. Invest. 101:1084-1093. crossref(new window)

5.
Carre, W., X. Wang, T. E. Porter, Y. Nys, J. Tang, E. Bernberg, R. Morgan, J. Burnside, S. E. Aggrey, J. Simon and L. A. Cogburn. 2006. Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index. Physiol. Genomics 25:514-524. crossref(new window)

6.
Chang, W. J., C. H. Chen, W. T. K. Cheng and S. T. Ding. 2007. The effect of dietary docosahexaenoic acid enrichment on the expression of porcine hepatic genes. Asian-Aust. J. Anim. Sci. 20:768-774.

7.
Chen, K. L., T. Y. Hsieh and P. W. S. Chiou. 2006. Caponization effects on growth performance and lipid metabolism in Taiwan country chicken cockerels. Asian-Aust. J. Anim. Sci. 19:438- 444.

8.
Cheng, C. H., B. R. Ou, T. F. Shen and S. T. Ding. 2006. Effects of dietary algal docosahexaenoic acid oil supplementation on fatty acid deposition and gene expression in laying Tsaiya ducks. Asian-Aust. J. Anim. Sci. 19:1047-1053.

9.
Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate- phenol-chloroform extraction. Anal. Biochem. 162:156-159.

10.
Ding, S. T., R. L. McNeel and H. J. Mersmann. 1999. Expression of porcine adipocyte transcripts: tissue distribution and differentiation in vitro and in vivo. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 123:307-318. crossref(new window)

11.
Dixon, J. L., R. Chattapadhway, T. Huima, C. M. Redman and D. Banerjee. 1992. Biosynthesis of lipoprotein: location of nascent apoAI and apoB in the rough endoplasmic reticulum of chicken hepatocytes. J. Cell Biol. 117:1161-1169. crossref(new window)

12.
Hsu, J. M., P. H. Wang, B. H. Liu and S. T. Ding. 2004. The effect of dietary docosahexaenoic acid on the expression of porcine lipid metabolism-related genes. J. Anim. Sci. 82:683-689.

13.
Jolivet, P., C. Boulard, V. Beaumal, T. Chardot and M. Anton. 2006. Protein components of low-density lipoproteins purified from hen egg yolk. J. Agric. Food Chem. 54:4424-4429. crossref(new window)

14.
Lee, Y. P. 2006. Taiwan Country Chicken: a slow growth breed for eating quality. Proceedings Symposium 2006 Scientific Cooperation in Agriculture between Council of Agriculture (Taiwan, ROC) and Institut National de la Recherche Agronomique (France). pp. 121-132. Tainan, Taiwan.

15.
Lee, Y. P., L. T. Yeh and H. H. Huang. 1997. A study on the production system of Taiwan Country chicken: growth traits of three-strain cross Country chickens. J. Chin. Anim. Sci. 26:271-284.

16.
Nimpf, J., M. Radosavljevic and W. J. Schneider. 1989. Specific post-endocytic proteolysis of apolipoprotein-B in oocytes does not abolish receptor recognition. Proc. Natl. Acad. Sci. USA 86:906-910. crossref(new window)

17.
Rayl, E. A., B. A. Moroson and G. P. Beardsley. 1996. The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping. J. Biol. Chem. 271:2225-2233. crossref(new window)

18.
Ryan, K. J., N. Charlet-B and T. A. Cooper. 2000. Binding of PurH to a muscle-specific splicing enhancer functionally correlates with exon inclusion in vivo. J. Biol. Chem. 275:20618-20626. crossref(new window)

19.
SAS. 2001. SAS User's Guide: Statistics. SAS Institute, Inc., Raleigh. NC.

20.
Segrest, J. P., M. K. Jones, H. De Loof, C. G. Brouillette, Y. V. Venkatachalapathi and G. M. Anantharamaiah. 1992. The amphipathic helix in the exchangeable apolipoproteins: a review of secondary structure and function. J. Lipid. Res. 33:141-166.

21.
Schneider, W. J., R. Carroll, D. L. Sevenson and J. Nimpf. 1990. Apolipoprotein VLDL-II inhibits lipolysis of triglyceride-rich lipoproteins in the laying hen. J. Lipid. Res. 31:507-503.

22.
Spike, C. A., N. Bumstead, L. B. Crittenden and S. J. Lamont. 1996. RFLP mapping of expressed sequence tags in the chicken. J. Hered. 87:6-9.

23.
Sun, D. X., W. Dong, Y. Ying and Z. Yuan. 2005. Cloning and characterization of liver cDNAs that are differentially expressed between chicken hybrids and their Parents. Asian-Aust. J. Anim. Sci. 18:1684-1690.

24.
Tarugi, P., G. Ballarini, B. Pinotti, A. Franchini, E. Ottaviani and S. Calandra. 1998. Secretion of apoB- and apoA-I-containing lipoproteins by chick kidney. J. Lipid. Res. 39:731-743.

25.
Tirunagaru, V. G., L. Sofer, J. Cui and J. Burnside. 2000. An expressed sequence tag database of T-cell-enriched activated chicken splenocytes: sequence analysis of 5251 clones. Genomics 66:144-151. crossref(new window)

26.
Walzem, R. L., R. J. Hansen, D. L. Williams and R. L. Hamilton. 1999. Estrogen induction of VLDLy assembly in egg-laying hens. J. Nutr. 129:467S-472S.

27.
Wang, P. H., B. H. Liu, Y. H. Ko, Y. C. Li and S. T. Ding. 2004. The expression of porcine adiponectin and stearoyl coenzyme A desaturase genes in differentiating adipocytes. Asian-Aust. J. Anim. Sci. 17:588-593.

28.
Wang, H. C., Y. H. Ko, H. J. Mersmann, C. L. Chen and S. T. Ding. 2006. The expression of genes related to adipocytes in pigs. J. Anim. Sci. 84:1059-1066.

29.
Yen, C. F., Y. N. Jiang, T. F. Shen, I. M. Wong, C. C. Chen, K. C. Chen, W. C. Chang, Y. K. Tsao and S. T. Ding. 2005. Cloning and expression of the genes associated with lipid metabolism in Tsaiya ducks. Poult. Sci. 84:67-74.

30.
Yen, C. F., H. W. Lin, J. C. Hsu, N. C. Lin, T. F. Shen and S. T. Ding. 2006. The expression of pituitary gland genes in laying geese. Poult. Sci. 85:2265-2269.

31.
Yen, C. J. 2004. Comparison of follicle growth rate and oviposition interval among commercial Taiwan Country chicken, selected Taiwan Country chicken and Single-Comb White Leghorn. Master Thesis. National Chung-Hsing University, Taichung, Taiwan.