Advanced SearchSearch Tips
Discrimination of Korean Cattle (Hanwoo) Using DNA Markers Derived from SNPs in Bovine Mitochondrial and SRY Genes
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Discrimination of Korean Cattle (Hanwoo) Using DNA Markers Derived from SNPs in Bovine Mitochondrial and SRY Genes
Yoon, D.; Kwon, Y.S.; Lee, K.Y.; Jung, W.Y.; Sasazaki, S.; Mannen, H.; Jeon, J.T.; Lee, J.H.;
  PDF(new window)
In order to distinguish Korean cattle (Hanwoo) beef from the imported beef from Australia in Korean markets, DNA markers based on PCR-RFLP from mitochondrial genes and SRY gene were applied. A total of 2,826 beef samples comprising 1,495 Hanwoo and 1,331 foreign cattle breeds were obtained in Korea. An 801 bp fragment of the SRY gene on the bovine Y chromosome, a 343 bp fragment of ND4 gene and a 528 bp fragment of ND5 gene in the bovine mtDNA were amplified by PCR and digested with three restriction enzymes, MseI, HpyCH4III and Tsp509I, respectively. The results showed that Bos taurus (T) type was the majority in Hanwoo by combining three markers (99.5%). However, 78.2% of Bos indicus (I) type was observed in the imported beef samples. These results indicated that three markers used in this study will be used as valuable markers for discriminating imported beef against Hanwoo.
Hanwoo;Bos taurus;Bos indicus;PCR-RFLP;SRY;ND4;ND5;
 Cited by
소 Y 염색체 특이 Microsatellite를 이용한 품종별 대립유전자 빈도 분석,윤두학;박응우;조용민;정일정;임석기;

Journal of Animal Science and Technology, 2007. vol.49. 4, pp.429-436 crossref(new window)
한국재래닭 COI 유전자의 단일염기다형 분석,진선덕;서동원;심정미;백운기;정기철;장병귀;최강덕;이준헌;

한국가금학회지, 2009. vol.36. 1, pp.85-88 crossref(new window)
A GHR Polymorphism and Its Associations with Carcass Traits in Hanwoo Cattle,;;;;;;;

Genes and Genomics, 2009. vol.31. 1, pp.35-41
Discrimination of Korean Cattle (Hanwoo) with Imported Beef from USA Based on the SNP Markers,;;;;;;;;

한국축산식품학회지, 2010. vol.30. 6, pp.918-922 crossref(new window)
Identification of Recently Selected Mutations Driven by Artificial Selection in Hanwoo (Korean Cattle),;;;;;;;;;;

아세아태평양축산학회지, 2013. vol.26. 5, pp.603-608 crossref(new window)
AGHR polymorphism and its associations with carcass traits in Harrwoo cattle, Genes & Genomics, 2009, 31, 1, 35  crossref(new windwow)
Single Nucleotide Polymorphism Analysis of the COI Gene in Korean Native Chicken, Korean Journal of Poultry Science, 2009, 36, 1, 85  crossref(new windwow)
Anderson, S., M. H. L. de Bruijn, A. R. Coulson, I. C. Eperon, F. Sanger and I. G. Young. 1982. Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome. J. Mol. Biol. 156(4):683-717. crossref(new window)

Hai-Guo, J., Z. Yu-min and Z. Gou-li. 2005. Analysis of microsatellite DNA polymorphisms in five china native cattle breeds and application to population genetic studies. Asian-Aust. J. Anim. Sci. 18(12):1696-1700. crossref(new window)

Kim, T. H., D. H. Yoon, E. W. Park, H. Y. Lee, S. J. Oh, I. C. Cheong, T. Y. Thak, K. N. Kim and J. Y. Han. 2000. A study on genotyping frequencies of the bovine melanocortin receptor 1 (MC1R) in cattle breeds. Kor. J. Anim. Sci. Technol. 42(6):735-744.

Lee, S. S., Y. H. Yang, S. Y. Kang, W. Y. Oh, B. S. Yang, S. B. Ko, S. J. Oh and K. I. Kim. 2000. Comparison of the genotypes and frequencies of MSH receptor (MC1R) gene in Korean cattle, Cheju native black cattle, Japanese black and Japanese brown cattle. Kor. J. Anim. Sci. Technol. 42(3):253-260.

Mannen, H., M. Kohno, Y. Nagata, S. Tsuji, D.G. Bradley, J. S. Yeo, D. Nyamsamba, Y. Zagdsuren, M. Yokohama, K. Nomura and T. Amano. 2004. Independent mitochondrial origin and historical genetic differentiation in North Eastern Asian cattle. Mol. Phylogenet. Evol. 32:539-544. crossref(new window)

Sasazaki, S., T. Imada, H. Mutoh, K. Yoshizawa and H. Mannen. 2006. Breed discrimination using DNA markers derived from AFLP in Japanese beef cattle. Asian-Aust. J. Anim. Sci. 19(8):1106-1110. crossref(new window)

Sasazaki, S., H. Mutoh, K. Tsurifune and H. Mannen. 2007. Development of DNA markers for discrimination between domestic and imported beef. Meat Sci. 77(2):161-166. crossref(new window)

Yoon, D., E. W. Park, S. H. Lee, H. K. Lee, S. J. Oh, I. C. Cheong and K. C. Hong. 2005. Assessment of genetic diversity and relationships between Korean cattle and other cattle breeds by microsatellite loci. Kor. J. Anim. Sci. Technol. 47(3):341-354. crossref(new window)

Zhang, Y., D. Sun, Y. Yu and Y. Zhang. 2006. A Y-linked SNP in SRY gene differentiates Chinese indigenous swamp buffalo and introduced river buffalo. Asian-Aust. J. Anim. Sci. 19(9): 1240-1244. crossref(new window)