JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effects of Different Levels of Supplementary Alpha-amylase on Digestive Enzyme Activities and Pancreatic Amylase mRNA Expression of Young Broilers
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Different Levels of Supplementary Alpha-amylase on Digestive Enzyme Activities and Pancreatic Amylase mRNA Expression of Young Broilers
Jiang, Zhengyu; Zhou, Yanmin; Lu, Fuzeng; Han, Zhaoyu; Wang, Tian;
  PDF(new window)
 Abstract
Four hundred and forty 1-day-old Arbor Acre broilers were fed commercial starter diets with 0, 250, 750 and 2,250 mg/kg of an alpha-amylase preparation from 1 to 21 days of age to investigate the effects of an exogenous enzyme on growth performance, activities of digestive enzymes in the pancreas and anterior intestinal contents and pancreatic amylase mRNA expression. Body weight gain (BWG) and average daily gain (ADG) increased linearly (p<0.01) with increasing levels of supplementary amylase but feed conversion ratio (FCR) was not affected. There was a negative quadratic change of protease and amylase in the small intestinal contents with the increase of supplementary amylase level. The activity of intestinal trypsin was also increased (p<0.05). Lipase was unaffected by amylase supplementation (p>0.05). The pancreatic protease, trypsin, and lipase were not affected by exogenous amylase levels. Consistent with the tendency for a linear depression of amylase activity, pancreatic -amylase mRNA was down-regulated by dietary amylase supplementation. The present study suggested that oral administration of exogenous amylase affected activities of intestinal enzymes and the production of pancreatic digestive enzymes in a dose-dependent manner.
 Keywords
Amylase;Broilers;Growth Performance;Enzyme Activity;mRNA;
 Language
English
 Cited by
1.
Effects of Supplementing Different Levels of a Commercial Enzyme Complex on Performance, Nutrient Availability, Enzyme Activity and Gut Morphology of Broilers,;;;;;;;;;;;;

아세아태평양축산학회지, 2008. vol.21. 5, pp.692-700 crossref(new window)
1.
Effect of different amylases on the utilization of cornstarch in broiler chickens, Poultry Science, 2016, pew323  crossref(new windwow)
2.
Effect of enzyme preparation on egg production, nutrient retention, digestive enzyme activities and pancreatic enzyme messenger RNA expression of late-phase laying hens, Animal Feed Science and Technology, 2012, 172, 3-4, 180  crossref(new windwow)
3.
Functional patterns of exogenous enzymes in different feed ingredients, World's Poultry Science Journal, 2013, 69, 04, 759  crossref(new windwow)
4.
Starch digestion capacity of poultry, Poultry Science, 2014, 93, 9, 2394  crossref(new windwow)
5.
Arabinoxylan and Arabinogalactan Content in Different Spring Wheats, International Journal of Food Properties, 2014, 17, 4, 713  crossref(new windwow)
6.
Comparative effects of dietary carbohydrases without or with protease on the ileal digestibility of energy and amino acids and AMEn in young broilers, Animal Feed Science and Technology, 2013, 181, 1-4, 35  crossref(new windwow)
7.
Enhancing the nutritional value of soybeans for poultry through supplementation with new-generation feed enzymes, World's Poultry Science Journal, 2016, 72, 02, 307  crossref(new windwow)
8.
Energy and nutrient utilization of broiler chickens fed corn-soybean meal and corn-based diets supplemented with xylanase, Poultry Science, 2016, 95, 8, 1881  crossref(new windwow)
9.
Effect of exogenous xylanase, amylase, and protease as single or combined activities on nutrient digestibility and growth performance of broilers fed corn/soy diets, Poultry Science, 2016, pew297  crossref(new windwow)
10.
Effect of age and diet composition on activity of pancreatic enzymes in birds, Journal of Comparative Physiology B, 2013, 183, 5, 685  crossref(new windwow)
11.
Effects of natural and heat modified palygorskite supplementation on the laying performance, egg quality, intestinal morphology, digestive enzyme activity and pancreatic enzyme mRNA expression of laying hens, Applied Clay Science, 2015, 104, 303  crossref(new windwow)
12.
The effect of protease, amylase, and nonstarch polysaccharide-degrading enzyme supplementation on nutrient utilization and growth performance of broiler chickens fed corn-soybean meal-based diets, Poultry Science, 2014, 93, 7, 1745  crossref(new windwow)
 References
1.
Acamovic, T. 2001. Commercial application of enzyme technology for poultry production. World's Poult. Sci. J. 57:225-243. crossref(new window)

2.
Ashild, K. and L. S. Jerry. 1989. Influence of age on lipase, amylase, and protease activities in pancreatic tissue and intestinal contents of young turkeys. Poult. Sci. 68:1561-1568.

3.
Bedford, M. R. and K. Autio. 1996. Microscopic examination of feed and digesta from wheat-fed broiler chickens and its relation to bird performance. Poult. Sci. 75:1-14.

4.
Bedford, M. R. 2000. Exogenous enzymes in monogastric nutrition-their current value and future benefits. Anim. Feed Sci. Technol. 86:1-13. crossref(new window)

5.
Bird, F. H. 1971. Distribution of trypsin and ${\alpha}$-amylase activities in the duodenum of the domestic fowl. Br. Poult. Sci. 12:373- 378. crossref(new window)

6.
Brannon, P. M. 1990. Adaptation of the exocrine pancreas to diet. Annu. Rev. Nutr. 10:85-105. crossref(new window)

7.
Brock, F. M., C. W. Forsberg and J. C. Buchanan-Smith. 1982. Proteolytic activity of rumen microorganism and effects of proteinase inhibitors. Appl. Environ. Microbiol. 44:561-569.

8.
Cowieson, A. J. 2005. Factors that affect the nutritive value of maize for broilers. Anim. Feed Sci. Technol. 119:293-305. crossref(new window)

9.
Cowieson, A. J., T. Acamovic and M. R. Bedford. 2003. Supplementation of diets containing pea meal with exogenous enzymes: Effects on weight gain, feed conversion, nutrient digestibility and gross morphology of the gastrointestinal tract of growing broiler chicks. Br. Poult. Sci. 44:427-437. crossref(new window)

10.
Cowieson, A. J., T. Acamovic and M. R. Berford. 2006. Using the precision-feeding bioassay to determine the efficacy of exogenous enzymes- A new perspective. Anim. Feed Sci. Technol. 129:149-158. crossref(new window)

11.
Erlanger, B. F., N. Kokowski and W. Cohen. 1961. The preparation and properties of two new chromogenic substrates of trypsin. Arch. Biochem. Biophys. 95:271-278. crossref(new window)

12.
Glazer, B. and M. L. Steer. 1977. Requirements for activation of trypsinogen and chymotrypsinogen in rabbit pancreatic juice. Anal. Biochem. 77:130-140. crossref(new window)

13.
Gracia, M. I., M. J. Aranibar, R. Lazaro, P. Medel and G. G. Mateos. 2003. Alpha-amylase supplementation of broiler diets based on corn. Poult. Sci. 82:436-442.

14.
Iji, P. A., K. Khumalo, S. Slippers and R. M. Gous. 2003. Intestinal function and body growth of broiler chickens fed on diets based on maize dried at different temperatures and supplemented with a microbial enzyme. Reprod. Nutr. Dev. 43:77-90. crossref(new window)

15.
Inborr, J. 1990. Enzymes: catalysts for pig performance. Feed Manage. 41:22-30.

16.
Iwamori, M., Y. Iwamori and N. Ito. 1997. Sulfated lipids as inhibitors of pancreatic trypsin and chymotrypsin in epithelium of the mammalian digestive tract. Biochem. Biophys. Res. Commun. 237:262-265. crossref(new window)

17.
Kullka, R. and D. Duksin. 1964. Patterns growth and ${\alpha}$-amylase activity in the developing chick pancreas. Biochem. Biophys. Acta. 91:506-514.

18.
Lonergan, P., A. Rizos, A. P. Gutierrez, P. M. Moriera, B. Pintado and J. de la Fuente. 2003. Temperal divergence in pattern of messenger RNA expression in bovine embryos cultured from the zyogte to blastocyst stage in vitro or in vivo. Biol. Reprod. 69:1424-1431. crossref(new window)

19.
Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193:265-273.

20.
Mahagna, M., I. Nir, M. Larbier and Z. Nitsan. 1995. Effect of age and exogenous amylase and protease on development of the digestive tract, pancreatic enzyme activities and digestibility of nutrients in young meat-type chicks. Reprod. Nutr. Dev. 35:201-212. crossref(new window)

21.
Murai, A., S. Satoh, J. Okumura and M. Furuse. 2000. Factors regulating amylase secretion from chicken pancreatic acini in vitro. Life Sci. 66:585-591. crossref(new window)

22.
Nitsan, Z., G. Avraham, Z. Zoref and I. Nir. 1991. Growth and development of the digestive organs and some enzymes in broiler chicks after hatching. Br. Poult. Sci. 32:515-523. crossref(new window)

23.
Nitsan, Z. and Z. Madar. 1978. The level and origin of amylase (EC 3.2.1.1) in the digestive tract of chicks receving trypsin inhibitors in their diet. Br. J. Nutr. 40:235-241. crossref(new window)

24.
Noy, Y. and D. Sklan. 1995. Digestion and absorption in the young chick. Poult. Sci. 74:366-373.

25.
Osman, A. M. and N. I. Tanios. 1983. The effect of heat on the intestinal and pancreatic levels of amylase and maltase of laying hens and broilers. Biochem. Physio. 75A:563-565.

26.
Onderci, M., N. Sahin, K. Sahin, G. Gikim, A. Aydin, I. Ozercan and S. Aydin. 2006. Efficacy of supplementation of ${\alpha}$-amylaseproducing bacterial culture on the performance, nutrient use, and gut morphology of broiler chickens fed a corn-based diet. Poult. Sci. 85:505-510.

27.
Piao, X. S., I. K. Han, J. H. Kim, T. Cho, Y. H. Kim and C. Liang. 1999. Effects of kemzyme, phytase and yeast supplementation on the growth performance and pollution reduction of broiler chicks. Asian-Aust. J. Anim. Sci. 12:36-41.

28.
Rideau, N., Z. Nitzan and P. Mongin. 1983. Activities of amylase, trypsin and lipase in the pancreas and small intestine of the laying hen during egg formation. Br. Poult. Sci. 24:1-8.

29.
Ritz, C. W., R. M. Halet, B. B. Self and D. M. Denbow. 1995. Endogenous amylase levels and response to supplementation feed enzymes in male turkeys from hatch to eight weeks of age. Poult. Sci. 74:1317-1322.

30.
Rothman, S., C. Liebow and L. Isenman. 2002. Conservation of digestive enzymes. Physiol. Rev. 82:1-18.

31.
Sarıcicek, B. Z., U. Kılıç and A. V. Garipoglu. 2005. Replacing soybean meal (SBM) by canola meal (CM): The effects of multi-enzyme and phytase supplementation on the performance of growing and laying quails. Asian-Aust. J. Anim. Sci 18:1457-1463.

32.
SAS. 1991. SAS User's Guide Version 6.03. SAS Institute, Cary, NC.

33.
Selle, P. H., V. Ravindran, G. Ravindran and W. L. Bryden. 2007. Effects of dietary lysine and microbial phytase on growth performance and nutrient utilisation of broiler chickens. Asian-Aust. J. Anim. Sci. 20:1100-1107.

34.
Somogyi, M. 1960. Modification of two methods for the assay of amylase. Clin. Chem. 6:23-27.

35.
Swanson, K. C., J. C. Matthews, A. D. Matthews, J. A. Matthews, J. A. Howell, C. J. Richards and D. L. Harmon. 2000. Dietary carbohydrate source and energy intake influence the expression of pancreatic ${\alpha}$-amylase in lambs. J. Nutr. 130:2157-2165.

36.
Verduin, P. A., J. M. Punt and H. H. Kreutzer. 1973. Studies on the determination of lipase activity. Clinica. Chimica. Acta. 46:11- 19. crossref(new window)

37.
Xu, M., J. H. Yao, Y. H. Wang and F. N. Wang. 2006. Influence of rumen escape starch on ${\alpha}$-amylase activity in pancreatic tissue and small intestinal digesta of lambs. Asian-Aust. J. Anim. Sci. 19:1749-1754.

38.
Wang, Z. R., S. Y. Qiao, W. Q. Lu and D. F. Li. 2005. Effects of enzyme supplementation on performance, nutrient digestibility, gastrointestional morphology, and volatile fatty acid profiles in the hindgut of broilers fed wheat-based diets. Poult. Sci. 84:875-881.

39.
Zanella, I., N. K. Sakomura, F. G. Silversides, A. Fiqueirdo and M. Pack. 1999. Effect of enzyme supplementation of broiler diets based on maize and soybeans. Poult. Sci. 78:561-568.