Advanced SearchSearch Tips
The Diversity of BoLA-DRB3 Gene in Iranian Native Cattle
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
The Diversity of BoLA-DRB3 Gene in Iranian Native Cattle
Nassiry, M.R.; Eftekhari Shahroudi, F.; Tahmoorespur, M.; Javadmanesh, A.;
  PDF(new window)
This study describes genetic variability in the BoLA-DRB3 gene in Iranian native cattle (Bos Indicus and Taurus) and relationships between these breeds. This is the first study of genetic polymorphism of the BoLA-DRB3 gene in Iranian native cattle. We examined exon 2 of the major histocompatibility complex (MHC) class II DRB3 gene from 203 individuals in four populations of Iranian native cattle (52 Sarabi, 52 Najdi, 49 Sistani, 50 Golpayegani cattle) using the hemi-nested PCR-RFLP method. We identified the 36 previously reported alleles and one novel pattern (*eac). Analysis of the frequencies of the various BoLA-DRB3.2 alleles in each breed indicated that DRB3.2*52 in Sarabi cattle (23%), DRB3.2 *14 and *24 alleles in Najdi cattle (13%), DRB3.2 *8 allele in Sistani cattle (22%) and DRB3.2*16 allele in Golpayegani cattle (14%), were the most frequent alleles. Allelic frequencies ranged from 1 to 23% among the 36 alleles and there were some alleles that were found only in Iranian cattle. Effective number of alleles in the four breeds was estimated to be 7.86, 11.68, 7.08 and 3.37 in Sarabi, Najdi, Sistani and Golpayegani, respectively. Observed heterozygosities were the highest in Sarabi (94%) and Najdi (94%). A population tree based on the frequency of BoLA-DRB3.2 alleles in each breed suggested that Najdi, Sarabi and Golpayegani cattle clustered together and Najdi and Sarabi were the closest breeds. Sistani cattle differed more from these three breeds. These new data suggest that allele frequencies differ between Iranian cattle breeds.
BoLA-DRB3;PCR-RFLP;Iranian Native Cattle;MHC;
 Cited by
Boom, R., C. J. A. Sol, M. M. M. Salimans, C. L. Jasen, P. M. E. Wertheim-Van Dillen and J. Van Der Noordaa. 1990. Rapid and simple method for purification of nucleic acids. J. Clini. Microb. 28(3):495-503.

Bradley, D. G., D. E. MacHugh, P. Cunningham and R. T. Loftus. 1996. Mitochondrial diversity and the origins of African and European cattle. Proceedings of the National Academy of Sciences USA, 93:5131-5135. crossref(new window)

Da Mota, A. F., J. E. Gabriel, M. L. Martinez and L. L. Coutinho. 2002. Distribution of bovine lymphocyte antigen (BoLADRB3) alleles in Brazilian dairy Gir cattle (Bos indicus). Europ. J. Immunog. 29:223-227. crossref(new window)

Dietz, A. B., N. D. Cohen, L. Timms and M. E. Kehril, Jr. 1977. Bovine lymphocyte Antigen class II alleles as risk factors for high somatic cell counts in milk of lactating dairy cows. J. Dairy Sci. 80:406-412. crossref(new window)

Gilliespie, B. E., B. M. Jayarao, H. H. Dowlen and S. P. Oliver. 1999. Analysis and frequency of bovine lymphocyte antigen DRB3.2 alleles in Jersey cows. J. Dairy Sci. 82:2049-2053. crossref(new window)

Giovambattista, G., C. D. Golijow, F. N. Dulout and M. M. Lojo. 1996. Gene frequencies of DRB3.2 locus of Argentine Creole cattle. Anim. Genet. 27:55-56. crossref(new window)

Hall, S. J. G. and D. G. Bradley. 1995. Conserving livestock breed biodiversity. Trends in Ecology and Evolution 10:267-270. crossref(new window)

Jeong, H. J., M. S. A. Bhuiyan, J. S. Lee, S. L. Yu, B. C. Sang, D. Yoon, J. T. Jeon and J. H. Lee. 2007. Characterization of BoLA-DRB3.2 Alleles in Hanwoo (Korean cattle) by Sequence Based Typing (SBT). Asian-Aust. J. Anim. Sci. 20(12):1791-1797.

MacHugh, D. E., R. T. Loftus, P. Cunningham and D. G. Bradley. 1998. Genetic structure of seven European cattle breeds assessed using 20 microsatellite markers. Anim. Genet. 29: 333-340. crossref(new window)

Mikko, S. and L. Andersson. 1995. Extensive MHC class II DRB3 diversity in African and European cattle. Immunogenetics 42 (5):408-413.

Nassiry, M. R., F. Eftekhari shahroudi, J. Mosafer, A. Mohammadi and E. Manshad. 2005. Analysis and frequency of bovine lymphocyte antigen (BoLA-DRB3) Alleles in Iranian Holstein cattle. J. Anim. Genet. 41(6):817-822.

Nassiry, M. R., F. Eftekhari shahroudi, J. Mosafer, A. Norouzy and A. Javadmanesh. 2004. Distribution of bovine lymphocyte antigen (BoLA-DRB3) alleles in Iranian Holstein cattle. 11th Asian-Australasian Animal Production Congress (AAAP) in Malaysia. 3:266-268.

Pashmi, M., S. A. Ghorashi, A. R. Salehi, M. Moini, A. Javanmard, S. Qanbari and S. Yadranji-Aghdam. 2006. Polymorphism of bovine lymphocyte antigen DRB3.2 alleles in Iranian native Sarabi cows. Asian-Aust. J. Anim. Sci. 19(5):775-783.

Sulimova, G. E., I. G. Udina and E. E. Karamysheva. 1997. BoLADRB3- associated resistance to persistant lymphocytosis in Russian dairy cattle. Proc. International conference on animal biotechnology 81-84.

Takeshima, S. N., M. Ikegami, M. Morita, Y. Nakai and Y. Aida. 2001. Identification of new cattle BoLA-DRB3 alleles by sequence-based typing. Immunogenetics 53:74-81. crossref(new window)

Takeshima, S., Y. Nakai, M. Ohta and Y. Aida. 2002. Short communication: Characterization of DRB3 alleles in the MHC of Japanese shorthorn cattle by polymerase chain reactionsequence- based typing. J. Dairy Sci. 85:1630-1632. crossref(new window)

Takeshima, S., N. Saitou, M. Morita, H. Inoko and Y. Aida. 2003. The diversity of bovine MHC class II DRB3 genes in Japanese Black, Japanese Shorthorn, Jersey and Holstein cattle in Japan. Gene. 316:111-118. crossref(new window)

Tavakkolian, J. 2000. An introduction to genetic resources of native farm animal in Iran. Dep of Ani Scie, Anim. Sci. Rese Ins, Karaj, Iran.

Udina, I. G., E. E. Karamysheva and G. E. Sulimova. 1998. Comparative analysis of Ayrshire and black pied cattle breeds by histocompatibility markers, Rus. J. Genetics. 34(12):1421- 1427.

Udina, I. G., E. E. Karamysheva, S. O. Turkova, A. R. Orlova and G. E. Sulimova. 2003. Genetic mechanisms of resistance and susceptibility to leukemia in ayrshire and black pied cattle breeds determined by allelic distribution of gene BoLA-DRB3. Russian J. Genet. 39(3):306-317. crossref(new window)

Van Eijk, M. J., J. A. Stewart-Haynes and H. A. Lewin. 1992. Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP. J. Anim. Genet. 23(6):483-496.